Exp 1

Biochemistry: Experiment 1
Experiment 1: Dilution And Molarity

Dilution problems:
Molarity (M) = mol / L
i.
How would you prepare:
a. 10 mL of a 1:10 dilution of a 1 M NaCl solution and what would be the final

concentration of NaCl?
Solution:
By using the equation M1V1 = M2V2
Then, the given information is been put into the equation.
M1 = 1 M
V1 = x
M2 = 1:10 x 1 M
M2 = 1:10 x 1 M
V2 = 10 mL
M 2V 2
M1
V1 =
= 0. 1 M
(0. 1 M )(10 mL)

1M
= 1mL
Hence, 1mL of the stock solution must be added up with 9 mL of solvent.

b. 80 mL of a 1:20 dilution of a 1 M NaCl solution?
Solution:
M1 = 1 M
V1 = x
M2 = 1:20 x 1 M
= 0. 05 M
mL
M2 = 1:20 x 1 M
V2 = 80 mL
V1 =
M 2V 2
M1
=
(0. 05 M )(80 mL)

1M
=4
Hence, 4 mL of the stock solution must be added up with 76 mL of solvent.

c. 50 mL of a 1:25 dilution of a 1 M NaCl solution?
Solution:
M1 = 1 M
V1 = x
M2 = 1:25 x 1 M
M2 = 1:25 x 1 M
V2 = 50 mL
V1 =
M 2V 2
M1
= 0.04 M
(0. 04 M )(50 mL )
1M
=2
mL
Hence, 2 mL of the stock solution must be added up with 48 mL of solvent.
ii.
How would you prepare exactly 6 mL of a 1/20 dilution (assume the concentration of
your starting solution is 1)?
Solution:
M1 = 1 M
V1 = x
M2 = 1:20 x 1 M
= 0.05 M
M2 = 1:20 x 1 M
V2 = 6 mL
V1 =
M 2V 2
M1
=
(0. 05 M )(6 mL)

1M
= 0.3
mL
Hence, 0.3 mL of the stock solution must be taken up and added up with 5.7 mL of
solvent.
iii.
You are provided with an antibody solution (Ab) that has a concentration of 600
microgram (g) / micriolitre (L). For lab, it is necessary to make the following
dilutions:
a. 10 L of 600 g/L Ab + 190 L of buffer to make a 1:20 dilution at _30__ g/L
Solution:
M1 = 600 g/L
V1 = 10 L
M2 =
=
M2 = x
V2 = 200 L
M 1V 1
V2
(600 g/ L)(10 uL)

200 L
= 30 g/L
V2 = 10 L + 190 L (stock solution + solvent)

b. 20 L of 1:20 Ab + 40 L of buffer to make 1:60 dilution at ___10___g/L
Solution:
M1
= 1:20 x 600g/L
V1 = 20 uL
M2
= 1:60 dilution at
______g/L
V2 = 60 L
M1 = 1:20 x 600g/ L
= 30 g/L
g/L
M2 =
=
M 1V 1
V2
(30 g/ L)(20 L)
60 L
= 10
OR
M2 = 1:60 x 600g/ L = 10 g/L
c. 5 L of 1:60 Ab + 5 L of buffer to make a _
1
_ dilution at _ 5 g/L
120
Solution:
M1
= 1:60 x 600g/L
M2
=x
V1 = 5 L
V2 = 10 L
M1 = 1:60 x 600g/ L
M2 =
= 10 g/L
M 1V 1
V2
(1 0 g/ L)(5 L)
10 L
g/L
Dilution factor (M2)
=
5
600
1
120
d. 10 L of 1:60 Ab + 90 L of buffer to make a _
1
____ dilution at _1_g/L
600
Solution:
=5
M1
= 1:60 x 600g/L
M2
=x
V1 = 10 L
V2 = 100 L
M1 = 1:60 x 600g/ L
M 1V 1
V2
M2 =
= 10 g/L
(1 0 g/ L)(10uL)
1 00 L
=1
g/L
Dilution factor (M2) =
1
600

e. 10 uL of 1:60 Ab + 40 L of buffer to make a _
1
300
_ dilution at _2_g/L
Solution:
M1
= 1:60 x 600g/L
M2
=x
V1 = 10L
V2 = 50 L
M1 = 1:60 x 600g/ L
M 1V 1
V2
M2 =
= 10 g/L
(1 0 g/ L)(10uL)
50 L
=2
g/L
=
2
600
1
300

f. 10 uL of 1:60 Ab + 10 uL of buffer to make a _5 g/L_ dilution at _
Solution:
1
__g/L
120
M1 = 1:60 x600g/L
M2 = x
V1 = 10L
V2 = 20 L
M1 = 1:60 x 600g/ L
M2 =
= 10 g/L
M 1V 1
V2
(1 0 g/ L)(10uL)
20 L
=5
g/ L
=
5
600
1
120
iv.
How much 2.0 M NaCl solution would you need to make 250 mL of 0.15 M NaCl
solution?
Solution:
M1 = 0.15 M
V1 = 250 mL
V2 =
=
v.
M2 = 2.0 M
V2 = x
M 1V 1
M2
(0.15 M )(250 mL)

2.0 M
= 18.75 mL
What would be the concentration of a solution made by diluting 45.0 mL of 4.2 M

KOH to 250 mL?
Solution:
M1 = 4.2 M
V1 = 45.0 mL
M2 = x
V2 = 250 mL
M2 =
=
vi.
M 1V 1
V2
(4.2 M )( 45.0 mL)

250 mL
= 0.756 mL
What would be the concentration of a solution made by adding 45.0 mL of 4.2 M

KOH to 250 mL?
Solution:
M1 = 4.2 M
V1 = 45.0 mL
M2 =
vii.
M 1V 1
V2
M2 = x
V2 = 45.0 + 250 mL
(4.2 M )(45.0 mL)

295 mL
= 0.641 mL
How much 0.20 M glucose solution can be made from 50.0 mL of 0.50 M glucose
solution?
Solution:
M1 = 0.50 M
V1 = 50.0 mL
V2 =
=
viii.
M2 = 0.20 M
V2 = x
M 1V 1
M2
(0.50 M )(50.0 mL)

0.20 M
= 125mL
What is the molarity of a solution that has 4.5 mol of solute dissolved in 300 mL of
solution?
Solution:
n = MV
n = 4.5 mol
V = 0.3 L
M=
=
M= x
n
V
4.5 mol
0.3 L
= 15 M
ix.
What is the molarity of a solution of NaOH that has 0.491 g dissolved in 400 mL of
solution?
Solution:
n=
mass
molar mass

n=x
Mass = 0.491 g
n=
M=
0.491 g
39.99
V = 400 mL
Molar mass (NaOH)
= 39.99
= 0.0123 mol
n
V
=
0.0123mol
400 mL
= 3.075 x 10 -5
x.
What is the molarity of a solution prepared by diluting 10.00ml of a 4.281 M solution

to 50.00 mL?
Solution:
M1 = 4.281 M
M2 = x
V1
V2 = 50.00 mL
= 10.00ml
M2 =
=
M 1V 1
V2
(4.281 M )(10.00 ml)

50.00 mL
= 0.856mL

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Biochemistry: Experiment 1

Experiment 1: Dilution And Molarity

How would you prepare:

a. 10 mL of a 1:10 dilution of a 1 M NaCl solution and what would be the final

(0. 1 M )(10 mL)

Hence, 1mL of the stock solution must be added up with 9 mL of solvent.

(0. 05 M )(80 mL)

Hence, 4 mL of the stock solution must be added up with 76 mL of solvent.

(0. 05 M )(6 mL)

(600 g/ L)(10 uL)

V2 = 10 L + 190 L (stock solution + solvent)

c. 5 L of 1:60 Ab + 5 L of buffer to make a _

V2 = 20 L + 40 L (stock solution + solvent)

d. 10 L of 1:60 Ab + 90 L of buffer to make a _

V2 = 10 L + 90 L (stock solution + solvent)

V2 = 10 L + 40 L (stock solution + solvent)

V2 = 10 L + 10 L (stock solution + solvent)

(0.15 M )(250 mL)

What would be the concentration of a solution made by diluting 45.0 mL of 4.2 M

(4.2 M )( 45.0 mL)

What would be the concentration of a solution made by adding 45.0 mL of 4.2 M

(4.2 M )(45.0 mL)

(0.50 M )(50.0 mL)

Then, the given information is been put into the equation.

What is the molarity of a solution prepared by diluting 10.00ml of a 4.281 M solution

(4.281 M )(10.00 ml)

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