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Sbi4u Molecgenetics lp3 Appendix1

DNA replication is the process by which a cell makes an identical copy of its DNA when it divides. It involves three main steps: (1) unwinding of the DNA double helix by helicase, (2) addition of nucleotides to each strand by DNA polymerase, and (3) ligation of the nucleotides together by DNA ligase to form the phosphodiester backbone. Errors in DNA replication can lead to mutations being introduced into new cells.

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165 views2 pages

Sbi4u Molecgenetics lp3 Appendix1

DNA replication is the process by which a cell makes an identical copy of its DNA when it divides. It involves three main steps: (1) unwinding of the DNA double helix by helicase, (2) addition of nucleotides to each strand by DNA polymerase, and (3) ligation of the nucleotides together by DNA ligase to form the phosphodiester backbone. Errors in DNA replication can lead to mutations being introduced into new cells.

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SBI4U: Molecular Genetics Name:__________________________________

DNA REPLICATION WORKSHEET

1. Why does DNA need to replicate?

2. In relation to the pictures below: Explain three main steps in the process of DNA replication.
Name the enzymes that go with each step.

a.

b.

c.

3. In which direction are new nucleotides added during replication?

4. What is the difference between and leading and lagging strand?

5. Which enzyme is responsible for unzipping the DNA double helix?


6. Which enzyme is responsible for facilitating the hydrogen bonding between nucleotides in a
new DNA molecule?

7. Which enzyme is responsible for creating the covalent bonds (specifically phosphodiester
bonds) that connect the sugar-phosphate backbone of the new DNA molecules?

8. Below is a single strand of DNA. Below each letter write the complementary strand of DNA.
ATGCGGCGA TTTA A GC

9. a. In the box A below, fill in the complementary strand of DNA to create a double strand.
b. Next to the box, using two different colored pens/pencil, create two new strands from the
original strand in the box A. Label which color represents the original strand and which color
represents the new strand.

Box A: Part b:
A
T
G
G
C
T
A
G

10. Describe the origin of each strand of the new double helices created after DNA replication.

11. Why do you think DNA replication important to the growth and development of a multi-cellular
organism?

12. What do you think would happen if the process occurred incorrectly?

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