5/21/2017 AntiStreptolysinO(ASO)Test:Principle,ProcedureandInterpretationmicrobeonline

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AntiStreptolysinO(ASO)Test:Principle,Procedure
andInterpretation 3.75/5 (4) Followmeon:

AUGUST8,2013BYTANKESHWARACHARYAINIMMUNOLOGY,LABORATORYDIAGNOSISOFBACTERIALDISEASE,SEROLOGY

5COMMENTS SUBSCRIBETOMICROBEONLINEVIAEMAIL

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Thetestprinciple,procedureandinterpretationpresentedherearebasedonLatexAntiStreptolysinO(ASO) andreceivenotificationsofnewpostsbyemail.
test.ThepresenceofAntistreptolysinOantibodiesinserumcanbeindicatedbyseeingtheabilityofserumto Join1,093othersubscribers
neutralizethehemolyticactivityofstreptolysinOreagent(antigen). EmailAddress

ItisarapidlatexagglutinationtestforthequalitativeandsemiquantitativedeterminationofantistreptolysinO Subscribe
antibodies(ASO)inserum.Ininfectionscausedbyhaemolyticstreptococci,streptolysinOisoneofthetwo
hemolyticexotoxinsliberatedfromthebacteriathatstimulatesproductionofASOantibodiesinthehuman
serum.Thepresenceandtheleveloftheseantibodiesinaserummayreflectthenatureandseverityof
infection.

TripleSugarIronAgar(TSI):
ASOlatexreagentisastabilizedbufferedsuspensionofpolystyrenelatexparticlesthathavebeencoatedwith Principle,Procedureand
StreptolysinO. Interpretation
july16,2013
WhenthelatexreagentismixedwithaserumcontainingASO,
agglutinationoccurs.Thesensitivityofthelatexreagenthas GramStaining:Principle,Procedure
beenadjustedtoyieldagglutinationwhenthelevelofASOis andResults
greaterthan200IU/ml,aleveldeterminedtobeindicativeof february2,2015
diseasebyepidemiologicalandclinicalstudies.Serahaving
titersofbetween200IU/mlto3500IU/mlwillbereactive.
BloodAgar:Composition,
Preparation,UsesandTypesof
SampleCollectionandHandling:Onlyfreshserum
Hemolysis
specimensshouldbeused.Plasmamustnotbeusedsince
august22,2013
fibrinogenmaycausenonspecificagglutinationofthelatex.It
ispreferabletotestsamplesonthesamedayascollected.
Catalasetest:principle,uses,
Serumsamplesmaybestoredat28Cforupto48hoursprior
ASOLatextest procedureandresults
totesting.Iflongerstorageisnecessary,serashouldbe
october7,2013
storedfrozenat20C.

MaterialsusedintheASOTest API20ETestSystem:Introduction,
ProcedureResultsandInterpretations
1.ASOAntigen:AstabilizedbufferedsuspensionofpolystyrenelatexparticlescoatedwithStreptolysinOand0.1%sodium may6,2015
azideaspreservative.Shakewellpriortouse.
2.ASOPositiveControl:Humanserumcontainingmorethan200IU/mlASOand0.1%sodiumazideaspreservative.
3.ASONegativeControl:Humanserumcontaining0.1%sodiumazideaspreservative.
4.Sufficientdisposablepipettes. CATEGORIES
5.Glasstestslide.
AntibioticResistance
Bacteriology
ProcedureforAntistreptolysinOTest: AnaerobicBacteriology
BacteriologyNote
1.Bringalltestreagentsandsamplestoroomtemperature. BiochemicaltestsinMicrobiology
2.Useadisposablepipettetodrawupandplaceonefreefallingdropofeachundilutedsampleintoitsidentifiedcircleofthe laboratorydiagnosisofBacterialDisease
slide.Retaineachpipetteformixinginstep5. MCQBacteriology
3.Deliveronefreefallingdropofpositiveandnegativecontrolintoitsidentifiedcircle. StructureofBacterialCells
4.MixtheASOlatexreagentbygentlyshaking.Addonefreefallingdropofreagenttoeachcontrolandsample. virulencefactorsofpathogenicbacteria
5.Usingtheflattenedendoftheappropriateplasticpipetteasastirrer(step2),thoroughlymixeachsamplewithreagent DifferenceBetween
withinthefullareaofthecircle. EmergingInfectiousDiseases
6.Discardthedisposablepipette. Immunology
7.Slowlyrocktheslideforexactlytwo(2)minutesandobserveforagglutinationunderahighintensitylight. MCQimmunology
8.Recordresults. serology
9.Rewashglassslideforfutureuse LifeofBidur
Microbialgenetics
TestResult:AtestsampleisconsideredtocontainASOantibodiesinexcessof200IU/mlwhenagglutination Microbiology
(clumping)isobservedwhencomparedtotheresultofthenegativecontrol. CultureMediausedinMicrobiology

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TankeshwarAcharya
Hello,thankyouforvisitingmyblog.IamTankeshwarAcharya.Bloggingismypassion,Iamworkingasa
lecturerandMicrobiologistatDepartmentofMicrobiologyandImmunology,PatanAcademyofHealth
Sciences,Lalitpur.Ifyouwantmetowriteaboutanypoststhatyoufoundconfusing/difficult,leaveinthe
commentsbelow.

LaboratorydiagnosisofMeningitisKeypoints MCQsinMicrobiology:HistoryofMicrobiology
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5thoughtsonAntiStreptolysinO(ASO)Test:Principle,Procedureand
Interpretation

abbas REPLY
April27,2015at8:45amEdit

informative

gulnawab REPLY
May7,2015at12:24pmEdit

informative

Boniface REPLY
February27,2017at4:17pmEdit

couldyoubehavingsoftcopybooksinthissitetodownload

Karo REPLY
April17,2017at6:59pmEdit

Anypossibilitytogetareliablesourceforthoseinformationtousetheminaseminarpaper?Verygoodinfolike
anoverviewbutsourcesneededthanks.

TankeshwarAcharya REPLY
April23,2017at12:37amEdit

DearKaro
Thankyouforyourinspiringcomment.Thisblogisonlyfortheeducation/academicpurposeto
facilitateundergraduatestudents,sonotwritteninthescientificways.Youcangetsimilarinformation
inpublishedpapersindexedinPUBMEDamongothers.

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