Biorad PW40 PW41 Manual ENG

I W PINILAB WASHERS PW40 : 85499
PW41 : 85508
K/V40 / PW41
Manuel d'Utilisation
User Manual
Y!
|]Code du manuel - Manual code : 87050
fe-N lot - Lot number: 9807 - 0003
Revision du manuel: Juillet 1998 - Logiciel versions 2.xx.2Y pour PW40 et PW41
Francais
Manual Revision : July 1998 - Software versions 2.XX.2Y for PW40 and PW41 -
English
'i'1
'Impression : 03/00
Rnnted 03/00 BIO-RAD
Sanofi Diagnostics Pasteur Plate Washers: PW40 & PW41
User Manual Codes 85499 & 8S508
TABLE OF CONTENTS
About this manual 4 3.7.3 Programming Synopsis 3/14
SAFETY INFORMATION 5 3.7.4 ADD 3/15
SAFETY SYMBOLS 6 3.7.4.1 ADD : KIT 3/15
3.7.4.2 ERRORS on KIT programming 3/16
FEATURES AND 3.7.4.3 ADD: METHOD 3/17
SPECIFICATIONS 1/1 3.7.4.4 ERROR on
METHOD programming 3/18
1.1 PW40 Basic features 1/1 3/19
3.7.4.5 ADD : PLATE
1.2 PW40 Outside dimensions [mrrV 1/2 3.7.5 INSERT 3/23
1.3 PW40 Hydraulic circuit 1/3 3.7.5.1 INSERT: KIT 3/23
1.4 PW41 Basic features 1/4 3.7.5.2 TEMPORARY KIT 3/23
1.5 PW41 Outside dimensions [mm] 1/5 3.7.5.3 INSERT: METHOD 3/23
1.6 PW41 Hydraulic circuit 1/6 3.7.5.4 INSERT: PLATE 3/24
1.7 Technical features 1/7 3.7.6 DELETE 3/25
1.7.1 Electrical Data 1/7 3.7.6.1 DELETE : KIT 3/25
1.7.2 Physical data 1/7 3.7.6.2 DELETE : METHOD 3/25
1.7.3 Hardware specifications 1/7 3.7.6.3 DELETE: PLATE 3/26
1.7.4 Software specifications 1/8 3.7.7 EDIT 3/27
1.8 Software parameters for Wash 3.7.7.1 EDIT: KIT 3/27
protocols 1/9 3.7.7.2 EDIT: PLATE 3/29
1.8.1 WASH protocol definition : 1/9 3.7.8 COPY 3/30
1.8.2 Methods 1/10 3.7.8.1 COPY: KIT 3/30
1.8.3 Parameter's definitions 1/12 3.7.8.2 ERROR on KIT copy 3/30
3.7.8.3 COPY: PLATE 3/31
2 INSTALLATION 2/1 3.7.8.4 ERROR on PLATE copy 3/31
2.1 Unpacking 2/1 3.7.9 Plate Test 3/32
3.7.10 RAM initialization 3/33
3 OPERATING
INSTRUCTIONS 3/1 4 MAINTENANCE &
3.1 Run Mode 3/1 TROUBLESHOOTING 4/1
3.1.1 How to use the Keyboard? 3/1 4.1 Routine maintenance
3.2 Microplate loading 3/2 Procedures 4/1
3.3 Select one KIT 3/3 4.1.1 Daily Maintenance 4/1
3.4 WASH interruption or 4.1.2 Washer storage conditions 4/1
Power Failure 3/5 4.2 Cleaning and disinfection 4/2
3.4.1 WASH interruption 3/5 4.2.1 Cleaning the manifold 4/2
3.4.2 POWER Failure 3/5 4.2.2 Disinfection 4/3
3.5 RINSE 3/6 4.3 Troubleshooting 4/4
3.5.1 RINSE (with PW40) 3/6 4.3.1 Hardware and software version 4/4
3.5.2 Please RINSE (with PW40) 3/6
3.5.3 RINSE (with PW41) 3/7 Appendix A/1
3.5.4 Automatic Rinsing (with PW41) 3/7 Accessories and
3.6 Configuration 3/8 spare part list A/1
3.6.1 Main menu 3/8 PW40 Wash protocols A/2
3.6.2 Washer's configuration 3/9 PW41 Wash protocols A/6
3.7 PROGRAMMING 3/12 Recommendations for
3.7.1 Kit Structure 3/12 optimized washing conditions A/10
3.7.2 Access to Revision Upgrade Description A/11
PROGRAMMING mode 3/13 Index A/12
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Version: July 1998
User Manual code : 87050
Sanofi Diagnostics Pasteur Plate Washers : PW40 & PW41
User Manual Codes 85499 & 85508
IMPORTANT
Please read carefully before using the PLATE WASHERS PW40 or PW41
This document is the copyright of SANOFI DIAGNOSTICS PASTEUR and must not be
copied or reproduced in any form without prior consent.
SANOFI DIAGNOSTICS PASTEUR reserves the right to make technical improvements
to this equipment and documentation without prior notice as part of a continuous pro-
gram or product development. This manual supersedes all previous editions.
Version: July 1998
User Manual code: 87050
L Sanofi Diagnostics Pasteur Plate Washers: PW40 & PW41
About this manual
This manual has been written for the laboratory technician and describes how to use
the PW40 and PW41 plate washers.
This manual is designed to give you the information you need to :
install the PW40 or the PW41
operate with the PW40 or the PW41 to suit your particular needs
perform the PW40 or the PW41 basic maintenance procedures, including disinfection.
This manual also describes all the features and specifications of the PW40 and PW41
hardware and configuration software.
You will find explanations of all the error messages and a problem solving guide in the
PW troubleshooting guide.
Version: July 1998
Sanofl Diagnostics Pasteur Plate Washers : PW40 & PW41
SAFETY INFORMATION
WARNING : To operate, this instrument must be mandatory connected to a
ELECTRIC wall socket equipped with earth.
SHOCK Furthermore the electrical line must be protected by an adapted
HAZARD fuse and a differential circuit breaker.
The access to the mains supply input plug, located on instru-
ment rear panel, must be kept free, in order to use this plug, if
necessary, as cut-off device.
Although this instrument is fully insulated and grounded, it is impor-
tant for all users to be aware of the potential hazards of using liquids
in close proximity to an electrical supply. In the event of any spillage,
the instrument must be immediately isolated from the electrical sup-
ply and drained. Spilt liquid should be removed as soon as possible.
Serious damage to the instrument may occur if fluid is allowed to
penetrate the case. The electrical supply MUST NOT be reconnected
until the instrument has been fully inspected by a qualified technician
or service engineer.
SERVICING MUST ONLY BE CARRIED OUT BY A QUALIFIED
TECHNICIAN OR SERVICE ENGINEER.
WARNING : In some operating modes (e.g. dispensing fluids or purging the sys-
SPILLAGES tem, particularly under automatic control) it is possible to overfill the
wells of a microplate. This can result in spillage of liquids and/or con-
tamination with hazardous substances. Care should be taken to en-
sure that overfilling does not occur.
If environmentally hazardous fluid is spilled, the instrument must first
be isolated from the electrical supply and physically isolated from the
working environment by placing it in a fume cupboard or similar loca-
tion. Contact the nearest service centre for assistance, advising them
of the health hazards associated with the spilled materials.
WARNING : OPERATING THIS EQUIMENT IN WAYS OTHER THAN DETAILED
INCORRECT IN THIS MANUAL MAY IMPAIR THE PROTECTION PROVIDED BY
OPERATION THE INSTRUMENT.
DO NOT operate this equipment with the covers removed - poten-
tially dangerous voltages are contained within.
DO NOT operate this equipment with the safety earth (ground) dis-
connected.
CAUTION DO NOT poke fingers, pencils or other implements into plate carrier
entry slot.
Version: July 1998
SAFETY INFORMATION
BE PREPARED for unexpected movement of the plate carrier and
noise from the Washer pump when the Washer is controlled by exter-
nal computer.
ENSURE that the main power cable is correctly wired. Colour codes
are as follows:
Europe United States
BROWN LIVE BLACK LIVE
BLUE NEUTRAL WHITE NEUTRAL
GREEN/YELLOW EARTH (Ground) GREEN GROUND
SAFETY The following alarm and information symbols can be found in several
SYMBOLS places in the washer. Only a qualified personal aware of the security
procedures is allowed to repair this instrument.
Please read this manual carefully before using this instrument.
Direct current
'">^ Alternating current
'"^s Direct and alternating current
Earth (ground) terminal
L
Protective earth (ground) terminal
On
O Off
|\ Caution (see enclosed documents)
Version: July 1998
ENSURE that all connections are tight and all blocking elements (foams,...) have been
removed before switching on the Washer.
If there is any doubt or concern about the safety of the instrument contact the nearest
approved service centre.
Version: July 1998
User Manual Codes 8S499 & 85508
FEATURES AND SPECIFICATIONS
PW40 1.1 PW40 Basic features
2x20 characters LCD Display
and 5 "soft" Keys
Aerosol Protection Cover
Wash bottle
Rinse bottle
Trap vial
Waste bottle
Manifold
8-Channel or
12-Channel
Removable
8-Mode/12-Mode Plate Carrier
Removable
Fig. 1 Autoclavable at 120C/250F
Version: July 1998
User Manual code : 87050 1/1
User Manual Codes 85499 &8B508
1 FEATURES AND SPECIFICATIONS
PW40 1.2 PW40 Outside dimensions [mm]
Washer's height: 243 mm (without bottles)
Fig. 2
Version : July 1998
User Manual code: 87050 1/2
PW40 1.3 PW40 Hydraulic circuit
Fig. 3
I
L
Version: July 1998
User Manual Codes 85499 & 8SS08
PW41 1.4 PW41 Basic features
Waste bottle
2x20 characters LCD Display
and 5 "soft" Keys
Aerosol Protection Cover
RS232 serial port for
connection to PC
4 liquid inputs
3 Wash bottles x
1 Rinse bottle
Manifold
2*8-Channel
Removable 8-Mode/12-Mode Plate Carrier
Removable
Autoclavable at 120C/250F
Fig. 4
Version: July 1998
User Manual Codes 85499 &8SS08
PW41 1.5 PW41 Outside dimensions [mm]
-530
368
I
822
Fig. 5
Version: July 1998
PW41 1.6 PW41 Hydraulic circuit
I
O
u
Fig. 6 s
"O
>
Version : July 1998
I
1.7 Technical features
1.7.1 Electrical Data
Voltage 240/100 VAC, 5 0 / 6 0 Hz
Consumption 100 VA max
Fuses 2,5 AT (2 fuses, 5x20 mm)
Power Cord on CEI socket
1.7.2 Physical data
Dimensions, including bottles
Width 551 mm,PW40
822 mm, PW41
Length 530 mm, Plate carrier out,
PW40 & PW41.
Height 300 mm, PW40
395 mm, PW41.
Weight 11,0 Kg, PW40
(without accessories) 11,6 Kg, PW41
Operating conditions 15-30 C /15 - 85 %R.H.
1.7.3 Hardware specifications
Manifold PW40: 8 or 12 channel (Default: 8 channel)
PW41: 8, 2*8, 3*8, 12 or 2*12 channel
(Default: 2*8)
Vacuum power PW40 1 integrated vacuum pump (maxi 8 L/min)
PW41 2 integrated vacuum pumps
(maxi 16 L/min).
Volume of WASTE bottle
PW40 : 2 litres
PW41: 8 litres
User interface: Flat keyboard with 5 diaphragm keys
2x20 characters LCD screen , backlighted
onPW41.
Version: July 1998
1.7 Technical features (continued)
1.7.4 Software specifications
Kits Up to 75 washing kits programmable.
Each kit is saved under its own name (up
to 15 characters) and is a combination of
methods repeated or not, with or without
soaking times, in strip mode or in plate
mode.
Method 6 single-cycle washing methods :
Wash, Aspiration, Dispensing, Bottom
Washing, Bottom Aspiration, Agitation.
4 two-cycle washing methods :
Wash + Aspiration, Wash + Bottom Aspi-
ration, Bottom Wash + Aspiration, Bottom
Wash + Bottom Aspiration.
Soak time 0 to 9,9 sec Strip mode
0 to 59 min 59 sec in Plate mode.
Repetition Every method can be repeated from 1 up
to 9 times.
Wash mode Strip mode or Plate mode.
Omit strip Strips can be omitted at kit programmation.
Plates Up to 10 plate parameter sets program-
mable.
Accepts flat or curved bottom microplates.
Vertical and horizontal speeds, vertical and
horizontal positions of the aspirating nee-
dle in relation with the wells are program-
mable.
Other features -Priming sequence of the hydraulic system
at change of Wash solution.
- Disinfection program of the hydraulic cir-
cuit.
- User programming can be inhibited.
Version : July 1998
1.8 Software parameters for Wash protocols
This section 1.8.1 WASH protocol definition :
describes the A WASH protocol, also called a KIT, is made of a succession of
Wash protocols, Wash Methods.
their methods and
their software There are 10 different wash methods available and every method is
parameters. programmed with its own batch of specific parameters.
It is useful to be Also, the Wash protocol is associated to a batch of plate param-
read prior starting eters -Positions- that are set in respect to plate geometry (flat, U or
to programme
V shaped well,...) as well as Speeds needed for the wash process
new wash
protocols or new
kits.
Wash protocol 75 Plate 10
or Positions
Kit 75 and
Speeds
Plate 1
Positions
Wash protocol 1 and
Speeds
Method 1
nW
The available methods are :
Method 2 Method Method Name
nAg Single-cycle Method
nW Wash
nA Aspiration
Method 3 nD Dispensing
nA nw Bottom Washing
na Bottom aspiration
nAg Plate Agitation
Two-cycle Method
Fig. 7 nW + A Wash + Aspiration
nW + a Wash + Bottom Aspiration
nw + A Bottom Wash + Aspiration
nw + a Bottom Washing + Bottom Aspiration
Version: July 1998
User Manual Codes 85499 8. 85508
1.8 Software parameters for Wash protocols (continued)
1.8.2 Methods
A method is a completely defined module made of one or two ele-
mentary cycles. Every kit is made of a succession of methods.
In a method, elementary cycles can be applied n times (n =1 to 9).
For method using two elementary cycles, only the first cycle is ap-
plied "n" time.
When repeated several times, the waiting time between elementary
cycles is called Soaking Time.
The waiting time between two methods is called Method Interval.
Whatever the selected mode, STRIP or PLATE (see definition be-
low), a method is always completely finished on the whole plate prior
starting the next method.
Available methods :
Method Abbreviated Name Method Name
on LCD Display
Single-cycle Method
nW WASH Wash
nA ASPIRATION Aspiration
nD DISPENSING Dispensing
nw BOTTOM WASH. Bottom Washing
na BOTTOM ASP. Bottom aspiration
nAg AGITATION Plate Agitation
Two-cycle Method
nW + A WASH + ASP Wash + Aspiration
nW + a WASH+BOT.ASP Wash + Bottom Aspiration
nw + A BOT.WASH+ASP Bottom Wash + Aspiration
nw + a B.WASH+B.ASP Bottom Washing + Bottom Aspiration
Kit parameters associated to method
MODE
STRIP or PLATE
IS"
STRIP mode: PLATE mode :
the total method is applied 'A the elementary cycle is
on the strip prior proces- applied successively on all
sing the next strip. strips prior applying next
elementary cycle.
Example: STRIP Mode PLATE Mode
Method 3W + Aon 1st strip W on the whole plate
3W+A 3W + A on 2nd strip W on the whole plate
etc... W on the whole plate
3W + A on last strip A on the whole plate
Version : July 1998
> FEATURES AND SPECIFICATIONS
1.8.2 Methods (continued)
Nr OF CYCLES Number of Cycles = n
1 to 9, step 1
number of elementary cycles that will be applied within the method.
SOAKING Soak time
Omn Osec to 59mn Osec, step 1 sec in PLATE mode.
Omn Osec to 9,9sec, step 0,1 sec in STRIP mode.
Waiting time between two consecutive elementary cycle within one
method.
MET. INTER Method Interval
Omn Osec to 59mn Osec, step 1 sec
Waiting time between two consecutive methods.
i ;
Version: July 1998
User Manual code: 87050 1 /11
1.8.3 Parameter's definitions
Parameters directly depending on KIT or Wash protocol (such as dis-
pensing, aspirating times, liquids, etc... are called "KIT Parameters",
Parameters depending on the microplate dimensions (mainly POSI-
TION and SPEED parameters) are called "PLATE parameters".
In the washer, the elementary cycles are as follows :
ASPIRATION The aspirating needle goes down and aspirates until reaching the
well bottom. The needle is shifted close to wall (Aspiration Horizontal
Position) for flat bottom well;
it remains centered for curved bottom well.
Programmed parameters :
Kit parameters :
CROSW. ASP. Crosswise aspiration (CWA)
(for flat bottom only)
YES or NO
the aspiration needle is shifted close to the wall (Aspiration Horizon-
tal Position) while going down to well bottom and aspirates; once the
aspiration time has elapsed, the aspiration needle is going up to ap-
prox middle of well height and is shifted to opposite end of wall (no
scratch on the bottom); the needle goes down to bottom and a se-
cond aspiration sequence takes place.
ASP. TIME Aspiration time
0,1 to 9,9 sec, step 0,1 sec
Time during which the aspirating needie is kept at the well bottom
and is aspirating.
Plate parameters :
ASP. HOR. POS. Aspiration Horizontal Position
0,0 (centered) to 2,0 mm, step 0,1 mm
(see also fig. 8 at the end of 1.8)
ASP.VERT.POS. Aspiration Vertical Position
0,1 to 15,0 mm, step 0,1 mm (see also fig. 8 at the end of 1.8)
0,1 is the highest needle position
15,0 is the lowest needle position
ASP. DOWNW. SPEED Aspiration Downward Speed
0 to 9, step 1. (speed mini = 0; speed max=9)
Version: July 1998
1.8.3 Parameter's definitions (continued)
D
DISPENSING The dispensing needle is placed over the well and dispenses a wash
solution in the well.
When the dispensed volume is higher than well capacity, the aspira-
tion needle aspirates the overflow to prevent liquid spilling on next
wells.
Programmed parameters ;
Kit parameters :
VOLUME (for one well)
50 to 3000 |iL, step 50 |il_
(the well volume is approx. 370 \iL)
OVERFLOW
1,0 to 12,9 mm, step 0,1 mm (see also fig. 8 at the end of 1.8).
This parameter sets the height of the aspirating needle and thus the
height of the liquid in the well.
12,9 is the lowest needle position (for the overflow).
It is recommended to dispense a volume slightly higher than wished
and have an overflow phase in order to obtain an equal distribution of
volume in each well; the slight flow rate variations among dispensing
needles are compensated by the overflow phase.
LIQUID
Wash W1 to Wash W9
When launching a kit in the RUN mode, the display will remind you to
connect correct Wash bottle prior starting.
FLOW flow rate compensation
-5 to +5, step 1.
The flow rate of the dispensing pump is adapted to the operating
manifold. However the flow rate can be slightly modified with this pa-
rameter (lower flow rate with negative value, higher flow rate with
positive value).
Plate parameters :
DISP. UPW. SPEED Dispensing Upward Speed
Version: July 1998
User Manual Codes 8S499 & 8SS0B
W
WASH Aspiration sequence followed by Dispensing sequence.
Programmed parameters:
Kit parameters:
CROSW. ASP. Crosswise aspiration
YES or NO (for flat bottom only)
See details page 1/10.
0,1 to 9,9 sec, step 0,1 sec. See details page 1/10.
50 to 3000 nl_, step 50 |iL
(the well volume is approx. 370 |xL)
OVERFLOW
1,0 to 12,9 mm, step 0,1 mm
LIQUID
Wash W1 to Wash W9 See details page 1/11.
-5 to +5, step 1. See details page 1/11.
Plate parameters
0,0 to 2,0 mm, step 0,1 mm
(see also fig. 8, at the end of 1.8)
0,1 to 15,0 mm, step 0,1 mm
(see also fig. 8, at the end of 1.8)
Version : July 1998
w Bottom Washing
BOTTOM the bottom of the well is thoroughly washed, followed by a normal
WASH. Wash (W) sequence.
Following sequences are applied :
-Downward aspirating movement of the needle into the well down to
Aspiration Vertical Position.
-Aspiration at Aspiration Vertical Position during Aspiration Time.
-One or two upward dispensing movements up to Bottom Wash Ver-
tical Position, dispensing at this position during a Bottom Wash Time
-...followed by downward aspirating movement down to Aspiration
Vertical Position.
-Dispensing up to Overflow Position.
In case of crosswise aspiration (for flat bottom only):
every aspiration sequence (close to the wall) is immediately followed
by an other aspiration sequence to the opposite end of wall as descri-
bed on page 1/10.
Kit parameters :
YES or NO for flat bottom only
L
50 to 3000 \iL, step 50 uL
(the well volume is approx. 370 |il_)
Volume is applied at the last Dispensing sequence.
OVERFLOW
1,0 to 12,9 mm, step 0,1 mm
Overflow is applied at the last Dispensing sequence.
LIQUID Wash W1 to Wash W9
-5 to +5, step 1. See details page 1/11.
Version: July 1998
User Manual Codes 8S499 & 85S08
1< FEATURES AND SPECIFICATIONS
w Bottom Washing (continued)
BOTTOM BOT. WASH NUMBER Bottom Wash Number
WASH. ! or 2
(continued) Number of Bottom Wash Occurence.
BOTTOM TIME Bottom Wash Time
0,1 to 9,9 sec, step 0,1 sec.
Time when dispensing at the Bottom Wash Vertical Position.
Plate parameters :
Aspiration Horizontal Position is applied for all Aspiration, Bottom Wash
and Bottom Aspiration sequences.
Aspiration Vertical Position is applied for all Aspiration sequences.
B.W.VERT.POS. Bottom Wash Vertical Position
0,1 to 15,0mm, step 0,1 mm (see also fig. 8 at the end of 1.8)
Bottom Wash Vertical Position is the height of the aspirating needle
during the dispensing sequence of the bottom wash.
This Speed is applied only at the first Aspiration sequence.
Dispensing Upward Speed is applied at the last Dispensing sequen-
ce.
BOT. DOWNW. SPEED Bottom Downward Speed
Speed of all downward movements for all bottom sequences (wash
and aspiration).
BOT. UPWARD SPEED Bottom Upward Speed
Speed of all upward movements for all bottom sequences (wash and
aspiration).
Version: July 1998
Bottom aspiration
BOTTOM ASP. following sequences are applied :
-Downward aspirating movement of the needle into the well down to
Aspiration Vertical Position.
-One or two upward aspirating movements up to Bottom (aspiration)
Position followed by downward aspirating movement down to Aspira-
tion Vertical Position.
In case of crosswise aspiration (for flat bottom only):
every aspiration sequence (close to the wall) is immediately followed
by an other aspiration sequence to the opposite end of wall as descri-
bed on page 1/10.
Programmed parameters
Kit parameters :
YES or NO for flat bottom only
See details page 1/10
BOT. ASP. NUMBER Bottom Aspiration Number
1 or 2
Number of Bottom Aspiration Occurence.
Plate parameters :
Aspiration Horizontal Position is applied for all Aspiration, Bottom Wash
and Bottom Aspiration sequences.
Aspiration Vertical Position is applied for all Aspiration sequences.
L
Version : July 1998
a Bottom aspiration (continued)
BOTTOM ASP. Plate parameters (continued):
(continued)
BOT.VERT.POS. Bottom (Aspiration) Vertical Position
0,1 to 15,0mm, step 0,1mm (see also fig. 8 at the end of 1.8)
15,0 is the lowest needle position.
Bottom (Aspiration) Vertical Position is the height of the aspirating
needle during the aspiration sequence of the bottom aspiration.
Aspiration Downward Speed is applied at the first Aspiration sequen-
ce.
BOT. DOWNW. SPEED Bottom Downward Speed
Speed of all downward movements for all bottom sequences
(wash and aspiration).
BOT. UPWARD SPEED Bottom Upward Speed
Speed of all upward movements for all bottom sequences
(wash and aspiration).
Version : July 1998
Ag Plate Agitation
The microplate is shaken horizontally during the Agitation T/mewith
AGITATION
an Agitation Amplitude and an Agitation Speed.
Kit parameters :
SHAKE TIME Agitation time
0,1 to 59,9 sec, step 0,1 sec.
Plate parameters :
SHAKING AMPLITUDE Agitation Amplitude
0 to 9, step 1
SHAKING SPEED Agitation speed
0 to 9, step 1
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Version: July 1998
User Manual Codes 8S499 & 85S08
Dispensing Needle
0 = HOME POSITION
Identification and range of para-
meters related to the location of
the needle in relation to the mi-
croplate well. r
Overflow Position
***** X*Xfl&UM^
1,0 to 12,9 mm
(Kit parameter)
Graduations in this sketch are Microplate
only for better understanding. Well
Set these parameters in real
conditions with real plate; well
dimension may vary from one
plate type to an other one.
Aspirating
Needle
Aspiration 10
Vertical Position
Bottom 11
(Aspiration)
Vertical Position
Bottom Wash 12
Vertical Position 4-
0,1 to 15,0 mm Aspiration Horizontal Position
(Plate parameters) 0,1 to 2,0 mm 13
(Plate parameter)
14
Fig. 8
2l 31 [mm
Version : July 1998
INSTALLATION
2.1 Unpacking
Carefully unpack accessories and washer.
IMPORTANT:
Do not lift up the washer
L by pulling on the foam frames!
First remove accessories at the top and beside the
washer.
Then, hold the washer (not the foams) and lift up.
Remove the foams, remove plastic bag and place
washer on a stable, horizontal surface.
Install bottles beside the washer as shown in user
manual on 1.1 for PW40 or 1.4 for PW41.
Fig.9
Fig.1O
Version: July 98
Sanof! Diagnostics Pasteur Plate Washers : PW40 & PW41
2 INSTALLATION
2.1 Unpacking (continued) In washer working area (Fig. 11,12 & 13):
-Remove adhesive tape blocking aerosol cover.
-Remove adhesive tape blocking plate carrier
to bottom plate (fig. 11).
Pull and push plate carrier to check that it
moves freely.
Manifold
-Remove the tie blocking the manifold on the
left side (fig. 12): push the tie to the left.
Fig.11 Remove the tie from the vertical shaft.
-Turn blocking device as shown on fig. 12 and
ensure to not hinder the manifold Up/Down
motion.
On rear panel :
Connect tubes on rear panel (match colours).
blue = Wash 1
gray Wash 2
green = Wash 3
Fig. 12 transparent = Rinse
red = to Waste bottle (liquid)
Yellow Vacuum to Waste bottle (air)
Vacuum pump protection :
Cut yellow tube at halfway between WASTE
bottle and washer and place hydrophobic filter
(supplied in maintenance kit).
Connect power cord.
Fuse installed : 2,5 A Slow Blow.
Fig.13
Plug Power Cord to mains socket; washer is
equipped with universal input switch mode
power supply (85 VAC to 264 VAC; 47 Hz to
440 Hz).
Turn the washer ON (ON/OFF switch on rear panel)
Whereas plate carrier and manifold are moving to their home posi-
tion, following message appears on display :
After initialization :
VERSION 2 . x x x . 2Y S E L E C T RUN
PW41 IN YES OUT
Fill one RINSE bottle with demineralized water and prime hy-
draulic circuit with at least 5 RINSE operations
(see RINSE mode, 3.5).
The washer is ready to use.
Version : July 98
OPERATING INSTRUCTIONS
3.1 Run Mode
3.1.1 How to use the Keyboard?
VERSION: 2 .x x x . 2 Y
PW41
S E L E C T : RUN
T i IN Y E S O U T
w
I Press "OUT" Key to set
Press"T"or"i"Keysto or remove microplate :
scroll main menu plate carrier moves out
See para 3.6.1 Main and is locked for 10 se-
Menu, page 3/8. conds to allow micropla-
te to be placed in carrier.
Press "YES" Key to
enter selected mode
Press "IN" key to move
Plate Carrier In
RUN: K I T 75
WASH + ASP. ESC
1
The function of the key is displayed
on the screen.
Depending on displayed menu,
- some keys are activated,
- some others have no function and
are therefore deactivated
Version: July 98
3/1
3 OPERATING INSTRUCTIONS
3.2 Microplate loading
Place the 96-well microplate on the plate carrier with "A1" corner resting
against metal spring on the right side (fig. 13).
A1 corner
Fig. 13
Version : July 98
3.3 Select one KIT
SE L E C T : R U N
t I IN YESOUT
RUN: KIT 01 Use "T" and ai" keys to select kit
T I YESESC
RUN : KI T
4-
75
t I Y S ESC
J
LAST STRIP 8 Use "T" and "4" keys to select last
t i YESESC strip to be processed
CONNECT THE WASH W9 for PW40 only : This message* is
displayed when the requested
ON YESESC wash buffer is not connected.
Once you have connected correct
WASH bottle, press "YES" and the
PUMP PRIMING pump will start to prime in order to
ESC operate with correct WASH liquid.
for PW41 : See ERR 21 on next
page.
R U N : K I T 75 Summary of the selected items :
STRIP 8 YESESC Kit name
Number of strip.
R U N : K I T 75 Once the kit is launched, the on-
WASH + ASP . ESC going method is displayed on
lower display line.
Press "ESC" to stop running kit.
L
R U N : K I T 75 Kit is terminated.
t 4- YESESC Display is back to previous kit
selection
B) : See 3.4.1
* : may appear as
"CONNECT THE WASH R9"
Version: July 98
3.3 Select one KIT (continued)
ERR:21 with PW41 :
SELECT:RUN
t I IN YESOUT
RUN: KIT 01
T i Y E S E S C
y
RUN : KI T 75
t i Y ES ESC
LAST STRIP 12
T i YESESC
ERR:21 WASH W9 NOT Error 21 indicates that Wash buffer
CONNECTED YES W9 (or R9) requested in "KIT 75"
is not connected.
Connect Wash buffer W9 (or R9)
at input 1, 2 or 3.
SELECT:RUN Then enter washer configuration
T i IN YESOUT to modify input assignment ac-
cordingly (see 3.6.2).
* : may appear as
"ERR:21 WASH R9 NOT
CONNECTED".
Version : July 98
3.4 WASH interruption or Power Failure
3.4.1 WASH interruption
When the "ESC" key is pressed whereas a kit is running, the follo-
wing message is displayed :
STOP THE TEST The elapsed time since the kit has
0H 3mnl7s RES ESC been stopped is displayed.
Press the "RES" key to resume the
kit at the point where it has been
stopped or
R U N : K I T 75
press the "ESC" key to go back to
WASH + A S P . ESC
main menu.

R U N : K I T 75
T 4- YESESC
A1 : Back to main menu
(See 3.3).
3.4.2 POWER Failure
When a power failure occurs whereas a kit is running, the following
message will be displayed when power is back :
POWER FAILURE The elapsed time since the kit has
0H43mn52s RES ESC been stopped is displayed.
Press the "RES" key to resume the
kit at the point where it has been
RUN: K I T 75 stopped or
ESC press the "ESC" key to go back to
WASH + ASP.
main menu.
RUN: KIT 75
t i YESESC
A) : Back to main menu
(See 3.3).
Version: July 98
3.5 RINSE
3.5.1 RINSE (with PW40)
SELECT: PRIME/RINSE
t 4- IN YESOUT
CONNECT THE RINSE Connect blue tube on RINSE
ON A YESESC bottle, ensure the plate carrier is
not removed,
then press "YES".
PRIMING/RINSING
E SC
SELECT:RUN
t i IN YESOUT
A) : Back to main menu
(See 3.3).
3.5.2 Please RINSE (with PW40)
The message
PLEASE RINSE
YES
is displayed to incite user to perform a RINSE operation when the
washer has not been used during 10 minutes after a WASH opera-
tion.
Press "YES" Key to go back to main menu and launch a RINSE.
IMPORTANT
The washer should never be switched off with WASH solution inside
the tubing and manifold.
Solid crystals from dried WASH solution could clog the dispense need-
les and clamp electrovalve tube.
Version : July 98
3.5 RINSE (continued)
3.5.3 RINSE (with PW41)
SELECT: PRIME/RINSE
T i IN YESOUT
J
SELECT WAY:A Way is mandatory "A" on PW41.
T I YESESC Press YES to continue.
INPUT SELECT. : 4
Select washer input according to
t 4- YESESC rinsing or priming that you want to
do.
INPUT 4 SOL.:RINSE Indication of the liquid connected
t i YESESC to selected input. If you change liq-
uid, use "T" or "I" keys to modify
input assignment (same as in
washer configuration, page 3/9).
Note:
-Input 4 is mandatory RINSE.
PRIMING/RINSING
ESC
SELECT:RUN
t I IN YESOUT
A ) : Back to main menu
(See 3.3).
3.5.4 Automatic Rinsing (with PW41)
PRIMING/RINSING
ESC
One automatic rinsing sequence will take place as soon as the time
set in "RINSE PARAM." (see 3.6.2. Washer's configuration) has
elapsed after the last wash.
Note :
When the washer won't be used for several days, it is recommended
to connect all inputs to rinse bottle and rinse every input as described
above in order to clean the pinch valve tube of every input. See also
4.1.2 Storage condition.
IMPORTANT
The washer should never be switched off with WASH solution inside
tubing and manifold.
Solid crystals from dried WASH solution could clog the dispense need-
les and clamp electrovalve tube.
Version: July 98 3/7
3.6 Configuration
3.6.1 Main menu
E L E CT : R UN RUN
T N YE 0 UT See 3.1 Run Mode
L/1
i
M
s E L E CT : P RI ME/ RI N s E PRIME/RINSE
T i I N Y ES 0 UT See 3.5 RINSE
s E L Ec T : D I SI NF ECT I 0 N DISINFECTION
t i I N Y ES 0 uT See 4.2.2 DISINFECTION
s ELEc T : C 0 N F I GURAT I 0 N CONFIGURATION
T 4 I N Y E S 0 UT See 3.6.2 Washer's configuration
s ELE c T : S E RV I C E/ V E R SERVICE/VER
t i N YES 0 uT See 4.3.1 Hardware and Software
version
Version : July 98
3.6 Configuration (continued)
3.6.2 Washer's configuration
Enter "CONFIGURATION" menu
SELECT: CONFIGURATION
T l I N YES OUT
GROUP : MANIFOLD Selection of Manifold
T 4 YESESC
MANIFOLD A: 2*8 Use "T" and "4" keys to set
type according to installed mani-
t 4 YESESC fold :
PW40:8-channel manifold
PW41 : 2*8-channel manifold.
PW 41 only
G R O U P '.VALVE PARAM. Assignment of Wash & Rinse
t 4 YESESC inputs on the rear panel
J
INPUT SELECT. : 1 Use "T" and "4" keys to select the
T -I YESESC input number
INPUT 1 S O L . : WASH Wl Use "T" and "4" keys to assign
T 4- YESESC wash (W1 to W9 or R1 to R9) or
rinse solution to selected input.
Note : input #4 is mandatory as-
signed to Rinse.
G R O U P : RIN SE PARAM. Setting of countdown prior to
T 4 YESESC launching automatic rinsing
A U T O R I N S E I N : 1 0 m n Use "T" and " I " keys to set count-
t 4 YESESC down (minutes),
from 0 to 240 minutes
Automatic Rinse is deactivated
when Rinse Time is set at "0".
GROUP : VALVE TYPE Selection of the multiwash
T 4- YESESC selector type on rear panel.
VAL TYP: MOTOR Default setting is "MOTOR".
T 4 YESESC Use "T" and "4" keys to select
L "PINCH" if the washer is equipped
with a 4 pinch valve system (old
device).
* : may appear as
(jGo to page 3/10 "INPUT 1 SOL.:WASHR1".
Version : July 98 3/9
3.6.2 Washer's configuration (continued)
(continuation from page 3/9 j
PW41 only
GROUP :VACUUM DET. Activation of the vacuum
T 4 YESESC detection
VAC. TRSH: 20pts Use "T" et "4" keys to set the
T 4 YESESC vacuum sensing threshold.
(Default value : 20 points.)
VACUUM DET ON A YES Use "T" and "4" keys to activate
t 4 YESESC (YES) or deactivate (NO) vacuum
detection.
GROUP : R S 2 3 2 PARAM Setting of RS232 serial port for
t 4 YESESC connection to PC.
BAUDS:9600 WAKE U P : Y Baud rate can be reduced in case
T 4 -> YESESC of transmission problem between
PC and washer.
Use "T" and "4" keys to set Baud
rate.
(Setting of Wake up function is ig-
nored)
GROUP :LIQUID DET. Activation of the liquid detec-
T 4- YESESC tion in wash and waste bottles
WASH LEVEL DET:YES Use "T" and "4" keys to activate
t 4 YESESC (YES) or deactivate (NO) liquid
level detector in Wash bottle, then
in Waste bottle.
WASTE LEVEL DET:YES
T J. YESESC
G R O U P : GE N E R A L Setting of dialogue interface
t 4 YESESC parameters.
J
LANG:ENGLISH BEEP:Y Use "T" and "4" keys to set
t 4 -> YESESC language, then "-" key and
"T" and "4" keys to activate (Y) or
deactivate (N) the Beep.
(j3otopage3/iO
Version : July 98
3.6.2 Washer's configuration (continued)
(continuation from page 3/10)
GROUP :Mini Prime Activation of a mini priming se-
t J, YESESC quence before every wash pro-
L tocol.
MiniPrime:Y V0L:13ml Use "T" and "I" keys to activate
t i -> YESESC (Y) or deactivate (N), then "->" key
and "T" and "I" to set the volume
from 1 to 30ml.
GROUP : S e cured Stop Activation of the secured stop
t . J. YESESC after every wash protocol to re-
move air from manifold.
SecurStop:N V0L:13ml Use "T" and "I" keys to activate
t i -> YESESC (Y) or deactivate (N), then "->" key
and "T" and "I" to set the volume
from 1 to 30ml.
L
(See 3.3).
Version : July 98
3.7 PROGRAMMING
3.7.1 Kit Structure
KIT PROGRAMME PLATE PROGRAMME
Parameters depending Parameters depending
on Kit (reagents) on Plate dimensions
KIT 75 PLATE 10
ni ATC *)
KIT 2
PLATE 1
KIT1 Name
(Kit) NAME Bottom Form
Plate Name Aspiration Horizontal Position
MANIFOLD
STRIP (omitted) Centering tion
METHOD
MODE IE Aspiration Vertical Position in
Crosswise Aspiration Bottom Aspiration vertical position
Aspiration Time Bottom Wash Vertical Position
(Dispensed) VOLUME >n)
OVERFLOW ER
Horizontal speed
LIQUID ME Vertical Speed
FLOW (compensation) \
Aspiration Speed In
BOT. WASH NUMBER Dispensing Speed Out
BOTTOM (Wash) TIME Bottom Wash Speed In
BOT. ASP. NUMBER Bottom Wash Speed Out
SHAKE TIME 7T
Nr OF CYCLES al)
Agitation amplitude
SOAK (time) Agitation Speed
END OF KIT?: NO I
MET(hod) INTER(val)
Fig. 15
n
METHOD -
MODE As seen on 1.8.3 Parameter's definitions
Crosswise Aspiration (KIT and PLATE), parameters directly de-
Etc... pending on KIT (such as dispensing, as-
END OF KIT?: YES pirating times, liquids, etc... are called "KIT
Parameters".
NR OF KIT? :
KIT INTER(val) Parameters depending on microplate
dimensions (mainly POSITION and
SPEED parameters) are called "PLATE parameters".
At programming, each kit is mandatory linked to one plate with the
"Plate number" parameter (Fig. 15).
This implies that the PLATE must be already existing or must be pro-
grammed prior the associated KIT is programmed.
Up to 10 PLATEs can be programmed.
Up to 75 KITs can be programmed.
Version : July 98
L Sanofi Diagnostics Pasteur Plate Washers : PW40 & PW41
3.7 PROGRAMMING (continued)
3.7.2 Access to PROGRAMMING mode
SELECT: RUN
t i IN YES OUT
Enter programming mode : press simultaneously
-"IN" and "OUT" keys.
P R G : A D D
T i YES
Version: July 98
3/13
SanofI Diagnostics Pasteur Plate Washers : PW40 & PW41
3.7.3 Programming Synopsis
Programming PRG : ADD ADD : KIT
Mode
See 3.7.4
ADD : METHOD
ADD : PLATE
PRG : INSERT INSERT: KIT
See 3.7.5
INSERT: METHOD
INSERT: PLATE
PRG : DELETE DELETE : KIT
See 3.7.6
DELETE : METHOD
DELETE : PLATE
PRG : EDIT EDIT : KIT
See 3.7.7
EDIT : PLATE
PRG : COPY COPY : KIT
See 3.7.8
COPY : PLATE
Fig.16
PRG:PLATE TEST RUN: KIT 75
See 3.7.9
Version : July 98
3.7.4ADD 3.7.4.1 ADD: KIT The kit is added at the end of the existing kit list
ADD KIT Use "T" and "I" keys to scroll the
YES ESC available menu.
t i Press 'YES" key to initiate the dis-
played selection.
Press "ESC" key to escape and
go back to former prompt.
N A M E : K I T N r2 Enter kit name : select character
t 4- -> YE S E with "T" and "4" keys, then press
"-" key to move to next charac-
ter, (up to 15 characters).
PLATE :PLATE01 1 Select plate to be associated to kit.
t i YESESC
J L
5
MANIFOLD: 8 Select manifold type:
PW40 : 8- or 12-channels
T I YESESC PW41 : 8-, 2*8-, 3*8- or 12-, 2*12-
J channels.
STRIP:1 X 3 4 5 6 Omit strip : place cursor on strip
T 4- -> YESESC with "->" key, then press "T" or "I"
key to have strip omitted ("X").
In RUN mode, the omitted strips
will never be processed.
STRIP:7 8 9 10 X 12
t i -> YESESC
METHOD :WASH + ASP. Select first method of kit.
T 4 YES ESC See method parameter selection
on 3.7.4.3.
t i
T ^ *1
E N D OF K I T : NO Answer "NO" in order to add an
t I YESESC other method.
MET . INTER : 0MN45 S Set "Method interval" with "T" or
YES ESC " i " and"-" keys.
(from Omn 0s up to 59mn 0s).
METHOD : DISPENSING
T i YESES
t
END OF
i
KIT:
YES
YES
ESCI
!i Answer "YES" when no more
T I YESESC methods have to be added.
p to page 3/16)
Version : July 98
3.7.4 ADD (continued)
3.7.4.1 ADD : KIT (continued)
Nr OF K I T S : 2 Select the Nr of times the kit has
T i YESESC to be run with "T" or "J/keys.
(up to 9 times).
KIT INTER: 0MN 0S When run more than once, set "Kit
t i -> YESESC interval" with "T" or " I " "-/keys,
(from Omn 0s up to 59mn59s).
SELECT:RUN The new kit is added at the end of
T i IN YES OUT the existing kit list.
Display is back to main menu.
3.7.4.2 ERRORS on KIT programming
ERR:24 TOO MANY KIT 75 kits are already programmed!
PROGRAMMED YES You must first delete an existing
kit prior programming a new one.
SELECT:RUN
t i IN YES OUT
ERR:32 KIT NAME You have entered no name or the
IMPOSSIBLE YES kit name you have entered is alrea-
dy existing. You are prompted to
J enter a new name to continue kit
programming.
NAME :
T 4, YES ESC
ERR:25 NOT ENOUGH Memory is full because one or se-
MEMORY YES veral kits are containing a large
number of methods.
You must first delete an existing
kit prior programming a new one.
S E L E C T : RUN
t I IN YESOUT
Version : July 98
3.7.4ADD (continued)
3.7.4.3 ADD : METHOD The method is added after the last method of the selected kit
ADD METHOD Use "T" and "I" keys to scroll the
available menu.
T YES ESC
Press "YES" key to initiate the dis-
played selection.
KIT: KIT Nr23 Select kit to which you want to add
t 4, YESESC a method with "t" and " i " keys
MET. INTER: 0MN 0S Set "Method interval" between last
T i -' YESESC existing method and added me-
thod with "T" or "4" and"-" keys,
(from Omn O&up to 59mn Os).
METHOD :WASH + ASP. Select method to be added
T i YESESC
MODE: PLATE Set kit parameters associated to
t I YESESC selected method.
See table next page and kit para-
CROSW. ASP.:NO meter definition 1.8.3.
t I YESESC
ASP. TIME : 0.5S
t I YES ESC
VOLUME: 750ul
t 4 YES ESC
OVERFLOW: 3.0mm
t i YES ESC
LIQUID : WASH W9
T i YESESC
FLOW : -3
T I YES ESC
Nr OF CYCLES: 2 Only the first cycle is played "n"
T i YESESC times (here twice) for a "two-
cycle" method such as Wash +
Asp (nW + A).
SOAKING 1MN 0S
T i YES ESC
L
S E L E C T :RUN
T i IN YESOUT
Version : July 98
3/17
3.7.4.3 ADD : METHOD (continued)
Summary of kit parameters associated to method.
CM
PC H
m
METHOD I
H
Cu
W
a
Method Code : c
Value See
Kit parameter def.
MODE : STRIP or PLATE p. 1/10
CROS. ASP. YES or p. 1/12
NO
ASP. TIME : 0,1 to p. 1/12
9.9 sec
VOLUME : 50 to p. 1/13
3000 |IL
OVERFLOW 1,0 to p. 1/13
12,9 ram
LIQUID : WASH Wl to p. 1/13
WASH W9
FLOW : -5 to p. 1/13
+5
BOT. WASH NUMBER: 1 or p. 1/16
2
BOTTOM TIME : 0,1 to p. 1/16
9.9 sec
BOT. ASP. NUMBER: 1 or p. 1/17
2
SHAKE TIME 0,1 to p. 1/19
59,9 sec
Nr OF CYCLES: 1 to p. 1/11
9
SOAKING: 0 to 59 mn in p. 1/11
PLATE mode
0 to 9,9 sec i
STRIP mode
3.7.4.4 ERROR on METHOD programming
ERR:25 NOT ENOUGH Memory is full because one or se-
MEMORY YES veral kits are containing a large
number of methods.
You must first delete an existing
S E L ECT : RUN kit or existing method prior
programming a new one.
t i IN YES OUT
Version : July 98
3.7.4.5 ADD : PLATE
The plate is added at the end of the existing plate list
ADD : PLATE Use "T" and "I" keys to scroll the
t I YES ESC available menu.
Press "YES" key to initiate the dis-
played selection.
NAME:PLATE 10 Enter plate name : select charac-
T -I - YESESC ter with "T" and "4" keys, then
press "-" key to move to next cha-
racter,
(up to 8 characters).
BOT. SHAPE: FLAT II Indicate well bottom shape :
t 4- YESESC || FLAT or CURVED
ASP.HOR. P O S . : 1 , 4 mm Aspiration horizontal position
t I YESESC (for FLAT bottom only)
See definition on para 1.8.3.
N
CENTERING : 0.3mm Centering of the opiate wells rela-
T I YESESC tively to manifold needle in the In/
Out direction
-2,0mm (IN) to +2,0 mm (OUT),
step 0,1 mm
Important parameter when a
crosswise aspiration must be
Bad Good Bad fc OUT made close to the wall of wells.
-2,0mrrr ^ +2,0mm
A S P . V E R T . POS. : 1 3 . 5 m m Aspiration vertical position.
T 4, YESESC See definition on para 1.8.3.
( j 3 o to page 3/2cP)
Version : July 98
3.7.4.5 ADD : PLATE (continued)
BOT.VERT.POS. :11.8mm Bottom (aspiration) vertical posi-
t I YESESC tion.
See definition on para 1.8.3.
.W.VERT.POS. :11.8mm Bottom Wash Vertical Position.
t I YESESC See definition on para 1.8.3.
HORIZONTAL SPEED : 6 Horizontal speed :
t i YESESC In / Out plate carrier speed during
non-work movements
(movement to home position, re-
turn movement from last to first
strip, e t c . ) .
0 to 9, step 1.
(speed mini = 0; speed max=9)
VERTICAL SPEED : 6 Vertical speed :
t 4, YESESC Up / Down manifold speed during
non-work movements
(movement to home position,
etc.).
0 to 9, step 1.
(speed mini = 0; speed max=9)
ASP. DOWNW. SPEED: 6 Aspiration downward speed.
T I YESESC See definition on para 1.8.3.
(J3o to page 3/2"P)
Version : July 98
3.7.4.5 ADD : PLATE (continued)
("continuation from page 3/20)
D I S P . U P W . SPEED : 6 Dispensing upward speed.
t i YESESC See definition on para 1.8.3.
It J r
BOT . DOWNW . SPEED: 3 Bottom downward speed
T I YESESC See definition on para 1.8.3.
BOT . UPWARD SPEED: 6 Bottom upward speed
T 4- YESESC See definition on para 1.8.3.
SHAKING AMPLITUDE: 2 Shaking amplitude
t i YESESC See definition on para 1.8.3.
SHAKING SPEED : 9 Shaking speed
t I YESESC See definition on para 1.8.3.
SEL.ECT:RUN
t 4- IN YESOUT
Version: July 98 3/21
3.7.4.6 ERRORS on PLATE programming
ERR:26 ALREADY 10 plates are already program-
10 PLATES!! YES med! You must first delete an ex-
isting plate prior programming a
j new one.
SELECT:RUN
t 4- I N YES OUT
ERR:33 PLATE NAME You have entered no name or the
IMPOSSIBLE YES plate name you have entered is
already existing. You are promp-
ted to enter a new name to conti-
nue plate programming.
NAME :
t i YES ESC
Version : July 98
3.7.5 INSERT
3.7.5.1 INSERT: KIT The new kit is inserted before another kit
INSERT KIT
t i YESESC
BEF.:KIT Nr23 Select kit before which you want
to insert new kit.
t i YESESC
NAME:KIT Nr24 Enter new kit name.
Then, same procedure as in
t i -> YESESC 3.7.4.1 add kit.
3.7.5.2 TEMPORARY KIT
RUN : T E M P O R A R Y KIT
T 4- YESESC
Power failure or erroneous OFF switching during KIT insertion will
cause the kit to be lost unless the first method has been completely
programmed. In this case the completely programmed methods can
be found in a kit named "TEMPORARY KIT". This kit is always inser-
ted at the first place of the kit list. It is then possible to COPY this
Temporary Kit and write its correct name, then continue with program-
ming of next methods and kit repetition parameters.
Once saved under its correct name, it is highly recommended to DELE-
TE the TEMPORARY KIT.
3.7.5.3 INSERT: METHOD
The new method is inserted before another method
INSERT METHOD
t i YES ESC
5
KIT: KIT N r23 Select kit where a method has to
t I YESESC be inserted.
B E F . : 1:WASH + ASP. Select method before which the
T I YESESC new method has to be inserted.
METHOD : DISPENSING Enter new method name.
t i YESESC
"1 Then, same procedure as in
3.7.4.3 add method.
Version: July 98
3.7.5 INSERT (continued)
3.7.5.4 INSERT : PLATE The new plate is inserted before another plate
IN S E RT : PLATE
t i YES E 5;c
1
B EF. * P L A T E 01 Select plate before which the new
YES E S>C plate has to be inserted.
t I
1
NA M E PI ATE 00 Enter new method name.
t i YES E 5>C
"I Then, same procedure as in para
3.7.4.5 add plate.
Version : July 98
3* OPERATING INSTRUCTIONS
3.7.6 DELETE
3.7.6.1 DELETE: KIT
DELETE : KIT
t 4- YESESC
KIT: KIT Nr24 Select kit to be deleted.
t I YESESC
DEL.: KIT Nr24 Press "YES" key to confirm kit is
T 1 YESESC deleted or press "ESC" key to es-
cape.
SE LE C T : R U N
T i IN YES OUT
3.7.6.2 DELETE : METHOD
DELETE : METHOD
T 4- YESESC
J
KIT: KIT Nr23 Select kit to which you want to de-
t
DEL.
4. IN YESESC
3:ASPIRATI0N
!i lete a method.
Select method to be deleted.
T 4- YESESC
METHOD : ASPIRATION Press "YES" key to confirm
t 4- YESESC method is deleted or
press "ESC" key to escape.
SELECT:RUN Note:
t 4- IN YESOUT deleting the method of a "one-
method" kit is equivalent to delete
this kit.
Version: July 98
3/25
3.7.6 DELETE (continued)
3.7.6.3 DELETE : PLATE
DELETE PLATE
T i YES ESC
PLATE :PLATE 10 Select plate to be deleted.
t J, IN YESESC
Press "YES" key to confirm plate
DEL.: PLATE 10 is deleted or
t 4- YESESC press "ESC" key to escape.
SELECT:RUN
t I IN YESOUT
3.7.6.4 ERROR on PLATE deletion
ERR:29 PLATE ASSOC. the plate is still associated with one
TO A KIT YES or several kits.
You must first associate an other
plate to affected kits or delete tho-
se kits prior deleting this plate.
SELECT:RUN
t J, IN YESOUT
Version : July 98
3.7.7 EDIT
3.7.7.1 EDIT: KIT
EDIT KIT
t 4- Y E S E S Cl
KIT: KIT Nr24 Select kit to be edited.
t -I YESESC
Select "MAIN PARAMETERS" or
"METHOD" with "T" and " 1 " keys.
EDIT:MAIN PARAMETERS EDIT:METHOD
T 4- YESESC t 4- YESESC
(jGo to page 3/28)
PLATE : P LATE 10 Edit main parameters:
t 4- YESESC Every displayed parameter can be
changed by using "T" or " I " keys.
J Example : the plate associated to
MANIFOLD : 8 this kit can be changed.
T 4- YESESC
J
STRIP:1 X 3 4 5 6
t I - YESESC
Nr OF K I T S : 2
t 4- YESESC
KIT INTER 0 M N 0S
t 4, YES ESC
J
VALID MODIFICATION Press "YES" to validate main pa-
T i YESESC rameters modification.
EDIT:MAIN PARAMETERS Press "T" or " I " keys until
t 4- YESESC "end of edit" message.
EDIT:END OF EDIT Press "YES" to quit EDIT mode.
t 4- YESESC; Press"T" or"I" keys to go backto
"Main Parameters" or "Method".
SELECT:RUN
T 4, IN YESOUT A ] : Back to main menu
(See 3.3).
L Version: July 98
3/27
3.7.7 EDIT (continued)
3.7.7.1 EDIT : KIT (continued)
^continuation from page 3/27)
EDIT:METHOD
t 4- Y E S E SC
M E T . : 4 : AS PI R A T I O N Select method to be edited.
t i Y E S E SC Then, every parameter of the
method can be modified by using
T Y ES ESC "T" or "4" keys.
Note that the "liquid : Wash Wx"
cannot be modified.
r
V A L I D M O D I FI Ci\T: C O N Press "YES" to validate method
Y ES ESC modification.
T i
E D IT : M E T H O D
-> Press "T" or "I" keys until
t I YESESC "end of edit" message.
EDIT:END OF EDIT Press "YES" to quit EDIT mode.
t i YESESC Press"T" or"I" keys to go back to
"Main Parameters" or "Method".
J
SELECT:RUN
T I IN YES OUT A ) : Back to main menu
(See 3.3).
C ) : Back to kit edition
(See 3.7.7.1).
Version : July 9B
3.7.7 EDIT (continued)
3.7.7.2 EDIT PLATE
ED I T : P LAT E
t 1 YES E5;c
1
4-
P L A TE : P LAT E 1 0 Select plate to be edited.
T i YE E <;c
i/i
1
B0 T 4-
HA P E : FL A T Every parameter of the selected
1/1
t Y E S E J>c plate can be modified by using

-
"T" or "4" keys.

|t 1 YES
r
E<>C
^r
1
VALI D
T I
M O D I F I C A T I o^J
Y E S E S>c
|
1 Press "YES" to validate main pa-
rameters modification.
S E L EC T : R UN
+
t 4. IN Y E s 01)T
(See 3.3).
Version: July 98
3.7.8 COPY
3.7.8.1 COPY : KIT

COPY : KIT
t i YESESC
KIT: KIT Nr24 Select kit to be copied.
T i YESESC
BEF.:KIT Nr26 Select kit before which you want
T i YESESC to place copy.
NAME:KIT Nr25 Enter copy name : select charac-
t 4- - YESESC ter with "T" and " I " keys, then
press "->" key to move to next cha-
racter,
(up to 15 characters)
SELECT:RUN
T i IN YES OUT
3.7.8.2 ERROR on KIT copy
E R R : 2 7 NO KIT No kit programmed!
PROGRAMMED YES You must first "add" a kit.
See also ERR 24
ERR 25
ERR 32
SELECT:RUN on 3.7.4.2.
t I IN YESOUT
Version : July 98
3.7.8 COPY (continued)
3.7.8.3 COPY: PLATE
COPY : PLATE
t i YESESC
J
PLATE :PLATE 05 Select plate to be copied.
t I YESESC
BE F . : P L A T E 05 Select plate before which you
t I YESESC want to place copy.
NAME:PLATE 0 4 Enter copy name : select charac-
t 4- -> YESESC ter with "T*1and " I " keys, then
press "-4" key to move to next cha-
racter,
(up to 8 characters)
S E L E C T : RUN
t I IN YESOUT
3.7.8.4 ERROR on PLATE copy
ERR:28 NO PLATE No plate programmed!
PROGRAMME D YES You must first "add" a plate.
See also ERR : 26
ERR:33
3.7.4.6.
S ELE C T:R U N
T i IN YES OUT
L Version: July 98
3.7.9 Plate Test
Step-by-Step RUN
The programmed kit can be used in a step-by-step mode i.e. manifold
and plate carrier are stopped at each programmed positions (Aspira-
tion horizontal position, Aspiration vertical position, Overflow position,
etc..) in order to check if these positions are correct. If not correct,
user must edit (see 3.7.7) the tested kit or its assigned plate in order
to modify position parameter(s).
PRG: PLATE TEST
t 4- YESESC
RUN:KIT 08 Use "T" or "I" keys to select kit to
Y E SE S C be tested.
T 4,
RUN:KIT 08 Press "YES" key to move to next
W A SH + A S P . YES ESC programmed position.
Keep the "YES" key pressed for a
I continuous motion (preferably
[WASH + ASP. YES ES deactive the beep).
The kit is processed on the whole
plate.
RUN:KIT 08
t 4. Y E SE S C
Version : July 98
3.7.10 RAM initialization
Every programmed kit and programmed plate are saved on a RAM
(Random Access Memory).
The contents of this MEMORY can be erased by a "RAM initializa-
tion" thus deleting the whole set of kits and plates that have been
programmed by the user.
Instrument software and DEMO kits and plates are not deleted as
they are saved in an EPROM not affected by RAM initialization.
How to perform the RAM initialization?
Turn the unit ON while keeping those keys pressed :
S E LE C T:R U N
t i IN Y E SO U T
1 1
Following message is displayed :
RAM INITIALIZATION Press "YES" to have the RAM
YES reset.
S E L E C T : RUN
t i IN YESOUT
L
L
Version : July 98
4* MAINTENANCE & TROUBLESHOOTING
4.1 Routine maintenance Procedures
4.1.1 Daily Maintenance
Prior starting to wash : manifold are not clogged.
It is recommended to pour an Otherwise, change pinch valve
appropriate disinfection solution tube, clean manifold (see 4.2.1
in the bottom of the WASTE bot- hereafter).
tle prior connecting it to the
washer. Thus, the infected liquid When washing is terminated :
coming from the washer and go- Rinse the washer . Use only
ing into the WASTE bottle will be deionized water for rinsing. Turn
deactivated. the washer off. Tubes and mani-
IMPORTANT: Avoid bleach fold will be kept wet with water
Fill the rinse bottle with deionized that will prevent them from clog-
water. Connect it to the washer. ging.
Launch a Rinsing sequence and Empty WASH bottle, rinse it thor-
check that pinch valve tube and oughly with deionized water.
4.1.2 Washer storage conditions
The washer should never be further dispensing of liquid.
stored with Wash solution inside In this case priming must be
dispensing tubes and manifold made with the help of a syringe
otherwise the liquid pump, the filled with deionized water con-
pinch valve tube and the mani- nected at the Rinse input of the
fold might be clamped if com- washer.
pletely dry, thus preventing any
Version: July 1998
4- MAINTENANCE & TROUBLESHOOTING
4.2 Cleaning and disinfection
4.2.1 Cleaning the manifold
The manifold is infectious. Dispense needle and chamber
Disinfect it prior any further Push the silicon cap from the
cleaning. chamber of the manifold using a
See disinfection procedure on stem or a screwdriver entered
next 4.2.2. from the opposite end of the cy-
lindrical chamber.
Aspirate needles and chamber Using one of the small pin sup-
Remove the manifold. plied in maintenance kit, clean
Using a duster or a non scratch- the small dispense needle.
ing utensil, thoroughly clean the Using the cylindrical brush sup-
outside of the dispense and as- plied in maintenance kit, thor-
pirate needles. oughly clean the small dispense
Remove the silicon top seal of chamber. Thoroughly rinse dis-
the manifold. pense chamber with deionized
Using the big pin supplied in water. Ensure that all impurities
maintenance kit, clean the big as- are removed from the manifold.
pirating needles. Plug dispense chamber of the
Rinse out the aspirate chamber manifold with the silicon plug.
with deionized or distilled water. Place it as close as possible to
Thoroughly clean the walls of the the first needle however without
aspirating cavity. blocking it. Extra silicon caps are
Look through the aspirate nee- supplied in the maintenance kit.
dles to ensure that cleaning is ad- Remount the manifold, replace
equate. the tubing and reprime.
Put the silicon top seal back in
its place.
Version : July 1998
MAINTENANCE & TROUBLESHOOTING
4.2 Cleaning and disinfection (continued)
4.2.2 Disinfection
The disinfection program applies to internal surfaces of tubing, connec-
tors, manifold and manifold needles.
SELECT : DISIN FECTION
T i IN YESOUT
CONNECT THE DISINF. Use disinfection solution approved
ON A YESESC by your laboratory. Connect the
tube(s) on rinse bottle cap. Fill
RINSE bottle with the disinfection
solution.
Ensure the plate carrier is not
PUMP PRIMING removed,
ESC Then press "YES".
DISINFECTION Pumps are stopped whereas
0H30mn00s ESC dispensing circuits are filled with
disinfection solution.
A countdown of 30 mn is started
after which you are prompted to
perform a RINSE operation.
IMPORTANT
The final RINSING sequence
must be performed in all cases
after the disinfection procedure
has been started (even for dem-
onstration purpose).
The software uses this final rins-
ing sequence to set the "Rinse" liq-
uid at all the 4 inputs.
The automatic rinsing will not
be possible (ERR 18) if rinsing
is not done after disinfection.
C O N N E C T THE RINSE Remove disinfection solution and
ON A YES ESC fill RINSE bottle with deionized
water (DiH2O)
Then press "YES"
PUMP PRIMING Then, prior launching a wash pro-
ESC tocol, assign the correct Wash so-
lution to the wash input(s) in the
CONFIGURATION menu or by
priming the circuit with the PRIM-
SELECT RUN ING/RINSING menu.
T i IN YES OUT
: Back to main menu
IMPORTANT:
Avoid bleach for disinfecting the washer as a thorough rinsing of the
tubes will not prevent traces of bleach to remain inside tubing and
manifold. This can be enough to corrupt reagents of next washing.
Disinfect the washer when it has not been used for more than one
day. When used daily, disinfect it at least once a week.
Disinfect the washer prior to any servicing or handling action, ship-
ment or change of site.
Version: July 1998 " tin
User Manual code: 87050 4/d
MAINTENANCE & TROUBLESHOOTING
4.3 Troubleshooting
4.3.1 Hardware and software version
SELECT: SERVICE/VER
T i IN YESOUT
GROUP :VERSIONS "T" and " I " keys are deactivated
t i YESESC on this prompt.
PW41 Hardware version.
T 4. Y E SE S C
VERSION 2.xxx.2Y Software version of the washer.
t I YESESC
SDPxxx Default User Data version
T 4- YESESC Kit list and
Plate list
programmed in factory and saved
on EPROM.
(Default user data are not deleted
by a RAM initialization).
: Back to main menu
(See 3.3).
Version : July 1998
Appendix
Accessories and spare part list
Part
Number Designation
82386 PW4X/LP35 WASTE BOTTLE 2L W/O CAP
82387 PW4X/LP35 COMPLETE WASTE BOTTLE 2L
82388 PW4X/LP35 WASTE 2L RUBBER CAP+TUBE
87042 PW4x COMPLETE WASH BOTTLE
87049 PW4x RINSE 2L SCREW CAP+TUBE
87053 PW4x WASH BOTTLE WITHOUT CAP
87054 PW40 WASH 2L SCREW CAP+TUBE
87058 PW4x RINSE BOTTLE WITHOUT CAP
87059 PW4x COMPLETE RINSE BOTTLE
87063 PW41 COMPLETE WASTE BOTTLE+DETECTOR
87086 PW41 SCREW CAP WASH BOTTLE+LEVEL DETECTION
87087 PW41 COMPLETE WASH BOTTLE+TUBE+LEVEL DETECTION
87088 PW41 COMPLETE WASTE BOTTLE 8L+LEVEL DETECTION
87067 PW41 WASTE 2L RUBBER CAP+LONG TUBE
87040 PW4x 12 CHANNEL MANIFOLD
87041 PW4x 8 CHANNEL MANIFOLD
87045 PW4x MANIFOLD CONNECTOR
87046 PW4x MANIFOLD CONNECTOR RINGS
87051 PW4x RUBBER SEAL FOR MANIFOLD
87055 PW41 2x12 CHANNEL MANIFOLD
87085 PW41 MANIFOLD CONNECTOR VACUUM SENSING
85509 PW40/LP35 COMPLETE TRAP VIAL
87082 PW40/LP35 TRAP VIAL CAP+TUBE
87083 PW40/LP35 TRAP VIAL WITHOUT CAP
87782 PW41 MOTOR SELECTOR TUBING SET
87094 PW4x/LP35 WASTE TUBING RED & YELLOW
87043 PW4x DISPENSING TUBE BLUE
87044 PW4x EXTERNAL TUBING SET
87047 PW4x PINCH VALVE TUBE
87060 PW4x DISPENSING TUBE RINSE TRANSPARENT
87061 PW41 DISPENSING TUBE WASH2 WHITE
87062 PW41 DISPENSING TUBE WASH3 GREEN
87064 PW41 4 WAY VALVE TUBE
87090 PW41 LINK TUBE 4WAY VALVE/UNIT
87091 PW41 SET TUBE MULTI WASH SELECTOR
87048 PW4x REMOVABLE uPLATE CARRIER
87081 PW4x HOLDER BOTTLE ON TOP WASHER
87089 PW41 HOLDER 2L.BOTTLES BESIDE WASHER
87004 PW41 FUSE 2-5AT 5X20 250V
85274 PW4X/LP35 HYDROPHOBIC FILTER 1|iM
87092 PW41 RS232 CORD
87052 PW40 STANDARD MAINTENANCE KIT
87093 PW MAINTENANCE KIT
87050 PW USER MANUAL F/E
Version: July 1998
User Manual code: 87050 A71
090Z.8 : opoo iBnueiAi jesn
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Kit name NAME
NAME
o o
o
PLATE
MANIFOLD
strip omtted
STRIP
METHOD
MODE
Crosswise Aspiration
CROSW. ASP
Aspiration time
CO CO
8? ASP. TIME
Dispensed volume
8
VOLUME
Overflow position
OVERFLOW
Selected liquid
LIQUID
Flow compensation
FLOW
Bottom Wash Number
BOT.WASH NUMBER
Bottom Wash Time
BOTTOM TIME
Bottom Aspiration Nbr
BOT. ASP. NUMBER
SHAKE TIME
Nr OF CYCLES
SOAKING
Method Interval
MET. INTER.
Nr OF KITS
Kit Interval
KIT INTER
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Kit name NAME
NAME
PLATE
MANIFOLD
strip omtted
STRIP
METHOD
5" MODE
CROSW. ASP
Aspiration time
ASP. TIME
Dispensed volume
VOLUME
Overflow position
OVERFLOW
Selected liquid
LIQUID
Flow compensation
FLOW
Bottom Wash Number
BOT.WASH NUMBER
Bottom Wash Time
8? BOTTOM TIME
BOT. ASP. NUMBER
SHAKE TIME
Nr OF CYCLES
SOAKING
Method Interval
MET. INTER.
Nr OF KITS
Kit Interval
KIT INTER
SlOdQS :N0ISIA3U
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QCKit
T21 N1 S1mn 800
Kit name NAME

NAME
i1
Flat 01
Flat 01

PLATE
00 00 MANIFOLD
strip omtted
I
>
STRIP
> to METHOD
Plate
Plate
Plate
Plate
j MODE
Yes
Yes
Yes
i o
CROSW. ASP
Aspiration time
0,5s
0,5s
0,3s
0,3s
I 01
ASP. TIME
Dispensed volume
i i
i
f VOLUME
Overflow position
1.0
i I
I OVERFLOW
Selected liquid
I i fg l
LIQUID
Flow compensation
i I * ! o
FLOW
Bottom Wash Number
i I i i I
BOT.WASH NUMBER
Bottom Wash Time
I j
i I
BOTTOM TIME
| j j I
BOT. ASP. NUMBER
i i i I I SHAKE TIME
Nr OF CYCLES
S? S S? j i SOAKING
Method Interval
I 3
MET. INTER.
- - Nr OF KITS
Kit Interval
i KIT INTER
:NOISIA3H SLOdaS
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_^
o to 00 en 01 CO ro
Flat 01
Flat 04
i Plate name
NAME
Bottom shape
Curved
Flat
Flat
BOT. SHAPE
Aspiration horizontal
i position
Vi
ASP. HOR. POS.
Plate centering
0,3
0,5
0,3
CENTERING
Aspiration vertical
14,5
12,0
CO position
"oi
ASP. VERT. POS.
Bottom aspiration
9,5
9,5
9,5
vertical position
BOT. VERT. POS.
Bottom Wash
to
9,5
9,5
vertical position
"oi
B.W. VERT. POS.
a> O) o> HORIZONTAL SPEED
CO CO CO VERTICAL SPEED
Aspiration downward
en speed
ASP. DOWNW. SPEED
Dispensing upward
to to to speed
DISP. UPW. SPEED
Bottom Wash downward
en en speed
BOT. DOWNW. SPEED
Bottom Wash upward
CO to to speed
BOT. UPWARD SPEED
SHAKING AMPLITUDE
to to to SHAKING SPEED
St-OddS ' NOISIA3U eiodcis
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0 S 0 8
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Kit name NAME
NAME
O o a
Cu
o
1 PLATE
MANIFOLD
strip omtted
STRIP
METHOD
MODE
CR0SW. ASP
Aspiration time
CO CO CO CO CO "CO
s? ASP. TIME
Dispensed volume
o
VOLUME
Overflow position
N
OVERFLOW
Selected liquid
LIQUID
Flow compensation
FLOW
Bottom Wash Number
BOT.WASH NUMBER
Bottom Wash Time
BOTTOM TIME
BOT. ASP. NUMBER
SHAKE TIME
Nr OF CYCLES
OAKING
Method Interval
MET. INTER.
Nr OF KITS
Kit Interval
KIT INTER
96/60/81.
: NOISIA3U
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Kit name NAME
NAME
PLATE
MANIFOLD
strip omtted
STRIP
METHOD
CO
MODE
CROSW. ASP
Aspiration time
en 01
ASP. TIME
Dispensed volume
VOLUME
Overflow position
! OVERFLOW
Selected liquid
LIQUID
Flow compensation
FLOW
Bottom Wash Number
BOT.WASH NUMBER
Bottom Wash Time
BOTTOM TIME
BOT. ASP. NUMBER
SHAKE TIME
Nr OF CYCLES
SOAKING
Method Interval
s MET. INTER.
Nr OF KITS
Kit Interval
KIT INTER
f lOdQS : NOISIA3H
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H
QCKit
T21 N1 Simn800
T18N1 S1mn800
T19N1 S1mn800
T17 N1 S1mn 800
T16 N1 S1mn 800
T15 N1 S1mn 800
CO
Kit name NAME
NAME
Flat 01
Flat 01
Flat 01
Flat 01
Flat 01
Flat 01
Flat 01
Flat 01
PLATE
CO en 0) o> o> 0) MANIFOLD
strip omtted
] j i i i i i i
STRIP
> S o> S > SI s > s > * CO METHOD
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
i MODE
z
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
i
o
o o 6= o 3 CROSW. ASP
p Aspiration time
0,5s
0,3s
0,3s
0,3s
0,3s
0,5s
0,5s
0,3s
0,3s
0,3s
0,3s
0,3s
0,5s
0,5s
0,3s
i Ol
ASP. TIME
00 00 Dispensed volume
!
I
i I i i i |
f r~ r-
|
r-
i g
f f VOLUME
Overflow position
; |
2,5
2,5
2,5
2,5
2,5
2,5
2,5
1,0
i i i I i
OVERFLOW
Selected liquid
j
i S i s ! s | s i s i I s s
LIQUID
Flow compensation
i i -pk | Ol
i Ol
i Ol i en | Ol i Ol ! Ol
FLOW
Bottom Wash Number
j I I i ] ] ro I | | - i i | j
BOT.WASH NUMBER
Bottom Wash Time
0,4s
! !
0,4s
0,4s
|
0,4s
| |
I i | i i |
! BOTTOM TIME
I i ! i | I i i I i i I
BOT. ASP. NUMBER
| !
10s
i i i i I i i i i SHAKE TIME
'
CO Nr OF CYCLES
8 o
8 ! i i i i !
I i I i | i 8 SOAKING
0) I
Method Interval
I
1mn
1mn
1mn
1mn
!
1mn
i 8 8 i 3 i i i i O
CO
MET. INTER.
- - - - - Nr OF KITS
Kit interval
I ! I i ! i
i KIT INTER
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o <o CO o> CJl *. CO M
o
Flat 01
Flat 04
i Plate name
NAME
Bottom shape
Curved
Flat
Flat
BOT. SHAPE
Aspiration horizontal
I position
Vi
*
ASP. HOR. POS.
Plate centering
e'o
0,5
0,3
CENTERING
Aspiration vertical
12,0
14,5
13,5
position
ASP. VERT. POS.
Bottom aspiration
9,5
9,5
9,5
vertical position
BOT. VERT. POS.
Bottom Wash
9,5
9,5
9,5
vertical position
B.W. VERT. POS.
o> cn cn HORIZONTAL SPEED
CO CD 00 VERTICAL SPEED
Aspiration downward
u cn cn speed
ASP. DOWNW. SPEED
Dispensing upward
to to to speed
DISP. UPW. SPEED
Bottom Wash downward
O) speed
BOT. DOWNW. SPEED
Bottom Wash upward
to to speed
BOT. UPWARD SPEED
SHAKING AMPUTUDE
to to to SHAKING SPEED
"I
HOddS :NOISIA3U
96/60/81.
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DATE: 31/05/96
REVISION: 03
Bad washing condition Factor generating risks How to restore good washing conditions? Comments
Inadequate aspiration Adjust Aspiration Parameters CO)
Too much residual volume in the wells Aspiration Vertical Position not set correctly ASPiration VERTical Position must be touching bottom of well to
Hornn Position JS3 CD (0 01
avoid aspirating needles blocked by foam (*)
'late parameter CO O
u zro
n
- Aspiration Horizontal Position not set cor- ASPiration HORizontal POSition must be as close as possible to 'late parameter
rectly well's wall.
Use CROSswise ASPiration Kit parameter Aspiration
Vertical Position o
Compensate possible horizontal offset with CENTERING parame- Plate parameter
ter
Aspiration Downward Speed not set correctly Reduce ASPiration DOWNward SPEED 'late parameter Aspiration Horizontal Position
O CD
One needle "forgets" temporarily to aspirate - High concentration of wetting agent in wash Respect dilution factor. Never use higher wetting agent
O CLZ5
buffer: needle might be blocked by foam concentration than required. Hydrophobic
Filter
5
Q . JD a
- Waste bottle is full Check waste bottle, ensure no foam goes on the yellow tube.
IS
Empty Waste Bottle prior starting to Wash
- Hydrophobic Filter is clogged Change Hydrophobic Filter Trap vial
Possibly install a trap vial
Waste bottle -
Contact your application specialist for advice CO CO
Inadequate dispensing
too strong. too weak - Flow Rate is not set correctly
Adjust Dispensing Parameters
Adjust FLOW rate compensation between -5 (weak) and +5
(strong)
Kit parameter s?
3-8
not enough quantity - Volume is not set correctly Adjust VOLUME between 50 to 3000 uL by step of 50 (iL Kit parameter
Always dispense slightly more than the awaited volume in the well
rrrrrrrr and aspirate the volume in excess by setting the OVERFLOW po-
sition (see below)
rJHUtft/tfh/W
not the same volume in every well - One or several dispensing needles must be Always ensure dispensing needles are clean
cleaned Use metallic cleaning pin supplied in maintenance kit
TTTUTTT
i" r"" ir r r r
onnonnnn - Overflow position not set correctly Adjust OVERFLOW position of the aspirating needle in order to
aspirate the excess of dispensed VOLUME
Kit parameter
03
CO
Often less volume in 1st strip than in the next High concentration of wetting agent in wash Respect dilution factor. Never use higher wetting agent
ones. buffer concentration than required. IT
(Manifold dripping in rinsing tank when on 0
standby before wash process) CO
- Rubber stopper in manifold dispensing "A" dimension for Rubber Stopper [mm]
Rubber Sti
chamber not set correctly. Shape of rubber stopper square round
Inzeoo This might create an excessive air bubble inside
Manifold channel 8
2*8
23<AS24
0
26<A<27
0
the dispensing chamber of manifold leading to 3*8 0 0
dripping 12 5<A<6 8<A<9 dispensing needles 2
2*12 0 0
- Manifold is dirty Clean manifold, rinse and dry it completely Cleaning Set supplied in Maintenance Kit
Spray silicone grease onto dispensing needles -Protect aspirating needles with adhesive tape
-Spray silicone grease on dispensing needle; ensure all the
outside surface of needle is treated.
-Clean inside of dispensing needles with metallic pin supplied
in maintenance kit. T3
-If still dripping: Activate "MiniPrime" and 'Secured Stop" to compensate and In Main menu, select "CONFIGURATION", available
minimize dripping in firmware equal or more recent than 1.50.2Y for PW40 CD
and 4.43.2Y for PW41 13
Manifold dripping during Wash process - Pinch Valve Tube is leaking Change pinch Valve tube spare supplied in Maintenance kit Q.
- Manifold connector with O-rings is leaking Ensure O-rings are correctly seated in manifold connector o 5
If still leaking, change O-rings. spare supplied in Maintenance kit
X"
Contact your application specialist for advice
User Manual , Codes 85499 & 85508
Appendix
Revision Upgrade Description
October 1996 First official release of the user manual compatible for both PW40 and PW41
washers.
July 1997 Implementation of the new motorized multiwash selector
Modified pages
u - page 1/6 1.6 LP41 Hydraulic circuit
- page 3/9 3.6.2 Washer's Configuration
Implementation of the selection between the new multiwash selector (MOTOR)
and the old one (PINCH valve).
Page 3/10 modified accordingly and page 3/11 created.
September 1997 Implementation of the new, internal design with integrated blocking device of
the manifold.
Modified pages
- page 2/2 2. Installation
April 1998 Modified warning message for electrical shock hazard.
Modified pages
- page 5
Bottles are removed from top and placed beside the washer.
Modified pages
- page 1/1,1/2,1/4,1/5,1/7 1 Features and Specifications.
- page 2/1 2.1 Unpacking.
Tie blocking the manifold on the left side is replaced by adhesive tape
(reason : the vertical guide is shorter.)
Implemented in serial product in May 98.
Modified pages
- page 2/2 2. Installation.
July 1998 "WASH Wx" may appear as" WASH Rx" on display in several firmware versions.
In the manual, the affected displays are marked with an "*" and the alternative
message is displayed on a foot note. The text associated to the displays mention
both "Wx" or "Rx" possibilities.
Modified pages
- page 3/3, 3/4 & 3/9
- page 3/11 (transfer of foot note "A: back to main menu" from page 3/9)
3* Operating Instructions.
Upgrade according to the configuration of the vacuum sensing (adjustable
vacuum threshold).
Modified page
- page 3/10 3.6.2. Washer's configuration.
Tie blocking the manifold on the left side is still used.
Modified page
- page 2/2 2. Installation.
Upgrade according to the new PW40 trap vial (Yellow tube is now transparent
and orange tube is now yellow).
Modified page
-page 1/3 1 * Features and Specifications.
Update of the accessories and spare part list.
Modified page
-page A/1 Appendix.
Version: July 1998
User Manual code: 87050 A/11
Sanofi Diagnostics Pasteur Plate Washers t PW40 & PW41
Appendix
Index
Symbols
2*8-Channel 1/4 Daily Maintenance 4/1
4 liquid inputs 1/4 DELETE 3/25
8-Channel 1/1 DELETE : KIT 3/25
DELETE: METHOD 3/25
DELETE : PLATE 3/26
Disinfection 4/3
Access to PROGRAMMING 3/13
DISP. UPW. SPEED 1/13
ADD : KIT 3/15
DISPENSING 1/13
ADD: METHOD 3/17
Dispensing Upward Speed 1/13
ADD : PLATE 3/19
Aerosol Protection Cover 1/1, 1/4
AGITATION 1/19
Agitation Amplitude 1/19 EDIT: KIT 3/27
Agitation speed 1/19 EDIT : METHOD 3/28
Agitation time 1/19 EDIT: PLATE 3/29
ASP. DOWNW. SPEED 1/12 Electrical Data 1/7
ASP. HOR. POS. 1/12 Electric Shock Hazard 5
ASP. TIME 1/12 End of Edit 3/27
ASP. VERT. POS. 1/12 ERR:24 3/16
ASPIRATION 1/12 ERR:25 3/16, 3/18
Aspiration Downward Speed 1/12 ERR:26 3/22
Aspiration Horizontal Position 1/12 ERR:27 3/30
Aspiration time 1/12 ERR:28 3/31
Aspiration Vertical Position 1/12 ERR:29 3/26
Autoclavable 1/1, 1/4 ERR:32 3/76
ERR:33 3/22
B ERROR on METHOD programming 3/18
B.W.VERT.POS. 1/16 ERROR on PLATE deletion 3/26
B.WASH+B.ASP 1/9, 1/10 ERRORS on KIT programming 3/16
Beep 3/10 ERRORS on PLATE programming 3/22
BOT.ASP. NUMBER 1/17
BOT.DOWNW. SPEED 1/16
BOT.SHAPE 3/19 FLOW 1/13
BOT.UPWARD SPEED 1/16 Flow rate compensation 1/13
BOT.VERT.POS. 1/18
BOT.WASH NUMBER 1/16 H
BOT.WASH+ASP 1/10 Hardware specifications 1/7
Bottom (Aspiration) Vertical Position 1/18 Hardware version 4/4
BOTTOM ASP. 1/17, 1/18 HORIZONTAL SPEED 3/20
Bottom Downward Speed 1/16 Hydrophobic filter 1/3
Bottom shape 3/19
BOTTOM TIME 1/16 I
Bottom Upward Speed 1/16
Bottom Wash + Aspiration 1/10 IN 3/1
Bottom Wash Vertical Position 1/16 INSERT 3/23
BOTTOM WASH. 1/15 INSERT: KIT 3/23
Bottom Washing + Bottom Aspiration 1/9, 1/10 INSERT: METHOD 3/23
INSERT: PLATE 3/24
K
Centering 3/19
Clean manifold 4/2 Keyboard 3/1
Compensation 1/13 KIT Parameter 1/12
Contents 2 Kit Structure 3/12
COPY : KIT 3/30
Crosswise aspiration 1/12
CROSW.ASP. 1/12 LIQUID 1/13
CWA 1/12 Liquid level detection in bottle 1/6
Version : July 1998
User Manual code : 87050 A/12
Appendix
Index (continued)
M
MAIN PARAMETERS 3/27 SAFETY INFORMATION 5
Maintenance 4/1 Secured Stop 3/11
Manifold 1/1 SHAKE TIME 1/19
MET. INTER 1/11 SHAKING AMPLITUDE 1/19
Method 1/10, 1/11 SHAKING SPEED 1/19
Method Interval 1/11 Soak time 1/11
Microplate loading 3/2 SOAKING 1/11
Mini Priming 3/11 Software parameters for Wash protocols 1/9
Software specifications 1/8
Software version 4/4
Omit strip 3/15 STRIP mode 1/10
OUT 3/1
OVERFLOW 1/13
Overflow Position 1/20 Technical features 1/7
Temporary Kit 3/23
Trap Vial 1/3
Physical data 1/7 Troubleshooting 4/4
Plate Agitation 1/19
Plate Carrier 1/1, 1/4
u
PLATE mode 1/10 Unpacking 2/1
PLATE parameter 1/12
PLATE TEST 3/32 V
Please RINSE 3/6
Vacuum sensing 1/6
Prime 3/3
Vertical speed 3/20
Programing Synopsis 3/14 VOLUME 1/13
PUMP PRIMING 3/3
PW40 Basic features 1/1 w
PW40 Hydraulic circuit 1/3
PW40 Outside dimensions 1/2 WARNING 5
PW41 Basic features 1/4 WASH 1/14
PW41 Hydraulic circuit 1/6 WASH + ASP 1/9, 1/10
PW41 Outside dimensions 1/5 Wash + Aspiration 1/10
Wash + Bottom Aspiration 1/10
WASH+BOT.ASP 1/9, 1/10
RAM initialization 3/33
Routine maintenance 4/1
RS232 serial port for connection to PC 1/4
Version: July 1998
User Manual code: 87050 A/13

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What are the main features of the PW40 and PW41 plate washers?

What are the safety considerations when using the plate washers?

I W PINILAB WASHERS PW40 : 85499

t Y E S E J>c plate can be modified by using

"T" or "4" keys.

T21 N1 S1mn 800

Kit name NAME

T17 N1 S1mn 800

T16 N1 S1mn 800

T15 N1 S1mn 800

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