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    SOP For OPERATION and Calibration of Incubator

    Uploaded by

    Tayyab Tahir
    The document outlines the standard operating procedure for operating and maintaining an incubator to ensure accurate temperature control for microbial growth. It details cleaning and calibration procedures to be done weekly, monthly, and annually by the microbiologist and accountability to the department head. Temperature should be recorded twice daily and any issues reported to quality control for troubleshooting or maintenance.

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    © All Rights Reserved
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    SOP For OPERATION and Calibration of Incubator

    Uploaded by

    Tayyab Tahir
    3K views3 pages
    The document outlines the standard operating procedure for operating and maintaining an incubator to ensure accurate temperature control for microbial growth. It details cleaning and calibration procedures to be done weekly, monthly, and annually by the microbiologist and accountability to the department head. Temperature should be recorded twice daily and any issues reported to quality control for troubleshooting or maintenance.

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    SOP for OPERATION and Calibration of Incubator

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    The document outlines the standard operating procedure for operating and maintaining an incubator to ensure accurate temperature control for microbial growth. It details cleaning and calibration procedures to be done weekly, monthly, and annually by the microbiologist and accountability to the department head. Temperature should be recorded twice daily and any issues reported to quality control for troubleshooting or maintenance.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    3K views3 pages

    SOP For OPERATION and Calibration of Incubator

    Uploaded by

    Tayyab Tahir
    The document outlines the standard operating procedure for operating and maintaining an incubator to ensure accurate temperature control for microbial growth. It details cleaning and calibration procedures to be done weekly, monthly, and annually by the microbiologist and accountability to the department head. Temperature should be recorded twice daily and any issues reported to quality control for troubleshooting or maintenance.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    SOP FOR OPERATION AND MAINTENANCE

    1.0 OBJECTIVE

    To ensure that the incubator performs satisfactorily and maintain accurate temperature for
    Microbial growth.

    2.0 SCOPE

    The SOP covers procedure for operation and calibration of incubator and is applicable to
    Quality Control Department formulation Units

    3.0 RESPONSIBILITY

    Microbiologist.

    4.0 ACCOUNTABILITY

    Department Head.

    5.0 PROCEDURE

    5.1 Ensure that the power supply to the incubator is switched OFF.

    5.2 Dedust the incubator daily externally with a clean dry cloth.

    5.3 Once a week remove adhered dust by wet mopping using detergent solution. Afterwards
    wipe the surface with a clean dry cloth to

    remove traces of detergent.


    5.4 Once in a month clean the interior surfaces with 2.5 % savlon solution using a clean cloth.

    6.0 OPERATING PROCEDURE

    6.1 Ensure that the incubator is properly connected to the power supply.

    6.2 Switch on the main .

    6.3 Turn on the red colour power knob towards 0-1.

    6.4 Turn on the cooling knob towards 0-1.

    6.5 To set the incubator at 22C , set the lower temperature 21 OC by pressing the SET POINT -
    1 and simultaneously adjust the

    temperature with the help of screw of SET and RST by screw driver.

    6.6 .Set the higher temperature 23 OC by pressing the SET POINT -2 and simultaneously adjust
    the temperature with the help of screw of

    SET and RST by screw driver.

    6.7 In the same manner the incubator can be set to 37, 44 and 55C whenever required by
    setting the lower temperature to 36,43 and 54 C respectively and by setting the higher
    temperature to 38,45 and 56 C respectively.

    6.8 Record the temperature twice daily. i.e. in the morning and in the evening. The temperature
    should not differ 2 C from the set

    temperature.

    7.0 Trouble shooting problems and remedial action :

    7.1 The temperature display is not glowing. Check for power supply and proper electrical
    connections of instruments with power point.

    7.2 Temperature is not even in the incubator. The air circulating fan may not be functioning.
    Check for it.

    7.3 Report any discrepancy observed during operation or temperature monitoring to Q.C.
    Executive and notify the defect to Maintenance Department.
    8.0 CALIBRATION

    8.1 Set the incubator temperature to 22C. Wait till the set temperature is reached.

    8.2 Take a calibrated thermometer and dip it in a 500 ml beaker filled to 3/4 of the volume with
    Glycerol AR grade.

    8.3 Keep the beaker inside, at the center of the incubator. close the incubator door. Allow the
    temperature to equilibrate for 30 minutes.

    8.4 Observe the temperature shown by the thermometer. The temperature shown by the

    display and by thermometer shall not differ by more than 0.5C.

    8.5 Record the temperature at two time intervals ( with a gap of 6 hours) in the temperature
    record.

    8.6 By following the same procedure as above carry out calibration by setting the incubator
    temperature to 37C, 44C and 55C.

    8.7 Record any discrepancy observed during operation or during temperature monitoring to
    Quality Control Executive and notify the defect to technical assistant for rectification. Affix
    BREAK DOWN label on the instrument.

    9.0 FREQUENCY OF CALIBRATION :

    9.1 Once a month and after each maintenance job.

    9.2 Record the calibration record in the format prescribed

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