Syllabus: "Quality Control in Microbiology"

Syllabus
For
Certificate Course
In
“Quality Control in Microbiology”
Coordinated by
Department Of Microbiology
Dhanaji Nana Mahavidyalaya, Faizpur
2013-14
Title of the Course: Certificate course in Quality Control in Microbiology.
Objective:
 To inculcate concepts in quality control in Microbiology
 To understand techniques for maintaining quality
 To increase employability skills of the students
 To comprehend laboratory safety and aseptic techniques
Duration of course : six months

Course structure :
1) Paper I - Quality Control and Management
2) Paper II- Laboratory course in Microbiology
Eligibility for admission: H.S.C. or equivalent qualification from recognized institution

Skeleton of the course:
Sr. Paper Name of Theory Teaching Maximum Marks Allotted Passing
No Subject /Practica Hours
l
External Internal Total External Internal Total
Paper I Quality Control Theory 45 80 20 100 32 08 40

and
Management
Paper Laboratory Practical 60 80 20 100 32 08 40

II Course In
Microbiology.
Coordinator External Expert Principal

Paper I: Quality Control And Management
Unit: I Concept of Quality Management (05 hr)
 Good manufacturing Practices

 Good laboratory Practices
 Quality Control
 Quality Assurance
 Audit and record
.Unit II General aspects of spoilage and Contaminants (10hr)

 Concept of contamination
 (Atmosphere, water, operator, raw material, packaging, buildings, equipments)
 General Characteristics of contaminates viz. Bacteria, fungi, virus etc
 Source of contamination, Air/ water micro flora
 Observation of contaminates - microscopy and staining
 Control / prevention of contamination
 Raw material, in process and final product
 Concept of Microbial spoilage
 Types & factors affecting on spoilage
 Preservation
Unit-III Laboratory preparations and cleaning strategies (07 hr)
 Normality, Molarity, Molality, Percent solution, ppm, ppb, Conversion of weight

 size, volume, Preparation of buffers and solution, Weighing and dissolving
 Making stock solution and its dilution, serial dilution
 Cleanliness in laboratory
 Cleaning of glassware’s
 Concept of Disinfection, Disinfectant, Sanitizer, Germicides, Bactericide
 Ideal Characteristics of Disinfectant
 Examples :- Phenolic compounds, Halogens, Heavy Metals etc
Unit- IV Sterilization (07 hr)
 Survivor curve, D, Z, F value

 Methods: Heat, Gaseous, Radiation, Filtration
 Sterilization control- Physical, chemical and biological indicators
 Disposal of microbial waste
 Classes of Manufacturing Area
 Instruments involved in aseptic handling
 Inoculations
 In process sampling
Unit-V Cultivations of Microbes (08 hr)
 Concept of growth
 Media- Liquid, Solid
 Isolation methods- Streak plate, Pour plate, Spread plate
 Biochemical Characteristics
 Cultivation of Fungi
 Maintenance of pure culture
Unit: VI Standard guidelines and Statistical reports (08 hr)
 WHO, FDA, BIS, ISO, NABL

 Pharmacopeia’s: IP, BP, USP
 Measures of central tendency and dispersion
 Probability and its distribution
 Concepts of hypothesis testing, correlation and regression
 Graphical representation of data
References:
1. Mansi E. and Miryce C., Fermentation Microbiology and Biotechnology, Taylor and Francis, London
(2002)
2. Casida, L. E. Industrial Microbiology, Wiley castern, New Delhi
3.Quality assurance of pharmaccuticals Vol I and 2, WHO, Geneva (2005)
4. Davleshina, T. A. Industrial fire safety guidebook, Willim Andrew Publishing (1998)
5. Koren H. and Bisesi, M. Handbook of Environemntal health and Saftey, Jaico publishing house, New
Delhi (1999)
6. Vyas S. P. and Dixit, V. K., Pharnaceutical Biotechnology, CBS public. New Delhi (1998)
7. Jogdon S. N. Industrial Biotechnology, Himalaya Publications, New Delhi (2006)
8. Patel A. H. Industrial Microbiology, McMillan Publication, New Delhi (2012)
9. Gupta A. Analytical Chemistry, PragatiPrakashan, Meerut (2006)
10. Sukumar De, Outline of Dairy Technology, Oxford Univ. press, New Delhi. (2005)
11. Frazier, W. C. and Westhoff D. C., Food Microbiology, Tata McGraw Hill, New Delhi(1995)
12. Dubey, R. C. and Maheshwari, A textibook of Microbiology, S. Chand, New Delhi (2004)
13. Quality Control Methods for Medicinal Plant material, WHO, Geneva (2002)
14. Hugo, W. and Russell, A. Pharmaceutical Microbiology 6th ed-Oxford, UK (2003)
15. Khan and Khanum, Fundamentals of Biostatistics, Ukaaz Publications (1994)
Paper II: Laboratory course in Microbiology
1. Demonstration of safety measures in laboratory
2. Precision and accuracy of pipette
3. Calibration of Balance and pH meter
4. Preparation of normal, molar, percentage solutions, ppm solution and labelling
5. Cleaning of Laboratory and Use and care of Microscope
6. Gram staining technique
7. Media preparation
8. Sterilization methods - dry and moist heat
9. Sterilization indicator for autoclave : Chemical and biological
10. Aseptic technique -Using personal hygiene, Bunsen burner, LAF, Biosafety cabinet
11. Growth Promotion Test for media
12. Isolation of bacteria-Using streak plate, Spread plate methods
13. Slide culture technique for fungal cultivation
14. Determination of Total Viable Count by Pour plate technique
15. Determination of Microbial limit test of finish product
16. Determination of Most probable number of water
17. Environmental monitoring by settling plate technique/ air sampler
18. Sterility test by membrane filtration method
19. Fumigation of laboratory
20. Use of MS office and MS excel for record maintenance
21. Industry visit
References:
1. Parija S, C., Textbook of Practical Microbiology, Ahuja Publishing House, Delhi (2007)
2. Adams, D. S., Lab Math, I. K. International, New Delhi (2004)
3. Dubey R. C. and Maheshwari, D. K., Practical Microbiology, S. Chand, New Delhi (2002)
4. Aneja, K. R., Experiments in Microbiology, plant pathology, Tissue culture, New Age
International publcationi, New Delhi (2
5. Swahney, S. K. and Singh R., Introduction to Biochcmistry, Narosa, New Delhi (2002)
6. Thimmaiah, S, R., Standard Mcthods of biochemical analysis, Kalyani Publication., Ludhiyan
(2006)
7. Jayaraman, J. Laboratory Manual in Biochemistry, New Age Int. New Delhi (2008)

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Syllabus

Duration of course : six months

Eligibility for admission: H.S.C. or equivalent qualification from recognized institution

External Internal Total External Internal Total

Paper I Quality Control Theory 45 80 20 100 32 08 40

Paper Laboratory Practical 60 80 20 100 32 08 40

Coordinator External Expert Principal

 Good manufacturing Practices

.Unit II General aspects of spoilage and Contaminants (10hr)

Unit-III Laboratory preparations and cleaning strategies (07 hr)

 Normality, Molarity, Molality, Percent solution, ppm, ppb, Conversion of weight

Unit- IV Sterilization (07 hr)

 Survivor curve, D, Z, F value

Unit: VI Standard guidelines and Statistical reports (08 hr)

 WHO, FDA, BIS, ISO, NABL

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