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K01813 - 20190318082703 - Lab Manual - Hanging Drop

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Asri Jefry

This document describes the hanging-drop procedure for observing bacterial motility under a microscope. The key steps are: 1) placing a drop of bacterial culture on a coverslip sealed over a depression slide, 2) inverting the slide to suspend the drop for viewing, and 3) examining the drop under low power to observe the sizes, shapes, and types of movement of bacteria in the hanging drop. The goal is to differentiate between true bacterial motility and Brownian movement.

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K01813 - 20190318082703 - Lab Manual - Hanging Drop

Uploaded by

Asri Jefry
72 views1 page
This document describes the hanging-drop procedure for observing bacterial motility under a microscope. The key steps are: 1) placing a drop of bacterial culture on a coverslip sealed over a depression slide, 2) inverting the slide to suspend the drop for viewing, and 3) examining the drop under low power to observe the sizes, shapes, and types of movement of bacteria in the hanging drop. The goal is to differentiate between true bacterial motility and Brownian movement.

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K01813_20190318082703_Lab manual_hanging drop

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This document describes the hanging-drop procedure for observing bacterial motility under a microscope. The key steps are: 1) placing a drop of bacterial culture on a coverslip sealed over a depression slide, 2) inverting the slide to suspend the drop for viewing, and 3) examining the drop under low power to observe the sizes, shapes, and types of movement of bacteria in the hanging drop. The goal is to differentiate between true bacterial motility and Brownian movement.

Copyright:

© All Rights Reserved
Download as DOCX, PDF, TXT or read online from Scribd
Download as docx, pdf, or txt
72 views1 page

K01813 - 20190318082703 - Lab Manual - Hanging Drop

Uploaded by

Asri Jefry
This document describes the hanging-drop procedure for observing bacterial motility under a microscope. The key steps are: 1) placing a drop of bacterial culture on a coverslip sealed over a depression slide, 2) inverting the slide to suspend the drop for viewing, and 3) examining the drop under low power to observe the sizes, shapes, and types of movement of bacteria in the hanging drop. The goal is to differentiate between true bacterial motility and Brownian movement.

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D) Hanging-Drop Procedure

Materials:
Glass slides (glass slide with depression)
Plasticine
Coverslip
Micropipette and tips
Microscope
Bunsen burner
Young broth cultures of bacteria

Methods:
1. Obtain a clean coverslip by its edges and carefully put some plasticine on its
corners.
2. Place the cover slip on a paper towel, with the plasticine side up.
3. ransfer 30-40µL of the bacteria broth culture in the center of the prepared
coverslip.
4. Place a depression slide over the drop on the coverslip so that the plasticine
seals the coverslip to the slide.
5. Quickly invert it so the drop is suspended and the drop can be observed hanging
from the coverslip over the concavity.
6. Examine the drop under low power by locating the edge of the drop, reduce the
light with the iris diaphragm and focus.
7. Look through the eyepiece and raise the objective slowly using the coarse
adjustment knob until the edge of the drop is observed as an irregular line
crossing the field.
8. Observe the different sizes, shapes and typpes of movement.
*Please make sure differentiate between true motility and Brownian movement
9. When finished, discard the coversipls in the disinfectant jar and clean the slides.

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