NIH Project Narrative Template: Content
NIH Project Narrative Template: Content
NIH Project Narrative Template: Content
1
NIH Research Instructions. (2018). pp. R-38. Retrieved from https://grants.nih.gov/grants/how-to-apply-application-
guide.html
2
Norins, L., & Matheson, S. (2014). NIH R15 Grant Application Mentor: An Educational How-to Manual (2nd edition).
Bonita Springs, FL: Principal Investigators Association.
structural characterization of… The structural data from this project will therefore be used to
guide…potentially providing new therapeutic agents for treating mycobacterial infections, as
well as chemical genetic probes to increase understanding of this enzyme system.”
Example Project Narratives from Norins and Matheson 2 (1, 2, and 4):
Example 1
Project Narrative: Despite the clear linkage between postmenopause and cardiovascular disease,
hormone replacement therapy has not proven to be cardioprotective. This application will identify the
novel G protein-coupled estrogen receptor 1 (GPER) as a novel calmodulin-binding protein and test the
overall hypothesis that GPER activation triggers in vascular endothelial cells and smooth muscle cells
feed-forward mechanisms that involve CaM both at the receptor level and downstream effectors, linking
signaling pathways mediated by different estrogen receptors. Effects of GPER activation will also be
tested on endothelial Ca2+ signaling and cell-cell interaction via modulation of calmodulindependent
activities. It is expected that the proposed studies will provide timely information that can help form the
basis for targeting estrogen receptor subtypes for preventive and therapeutic purposes.
Example 2 (This proposal has multiple PIs, and the Narrative emphasizes goals of the R15 program.)
Project Title: Chemoenzymatic synthesis of pradimicin analogues for novel antifungal and antiviral agents
Project Narrative: This project aims to generate a library of novel pradimicin analogs using a combination
of enzymatic and chemical methods, and evaluate the antifungal and antiviral activities of these
synthesized compounds. The objectives of this proposal are consistent with the goals of the NIH
Academic Research Enhancement Award (AREA) R15 program, and will have a significant impact on
public health by providing promising molecules for new anti-infective drug discovery. This multi-
departmental project will be conducted by a team of researchers consisting of three faculty members, a
technician, two graduate students, and four undergraduate students.
Example 3
Project Title: Mechanisms of cold storage lesion resistance in hibernating ground squirrel platelets
Project Narrative: The proposed research is clinically relevant as it could lead to the generation of cold
storage lesions in platelets, extending their availability for transfusions. By studying hibernating 13 -lined
ground squirrels, the project supports objective 1 of the strategic vision of the NHLBI to “understand
normal biological function and resilience” in exploring natural resilience to cold storage lesions in
platelets. By involving undergraduates in this project, both in and out of the classroom, the project
supports objective 8 to “further develop, diversify, and sustain a scientific workforce capable of
accomplishing the NHLBI’s mission.”
Example 4
Project Narrative: This study will yield a comprehensive structural characterization of the thioredoxin
enzyme system from Mycobacterium tuberculosis, the causative agent of tuberculosis. Since the
Mycobacterium tuberculosis thioredoxin system protects it from the oxidative attacks of human immune
cells, it is currently being pursued as a promising new drug target. The structural data from this project will
therefore be used to guide structure-based identification of inhibitors, potentially providing new
therapeutic agents for treating mycobacterial infections, as well as chemical genetic probes t o increase
understanding of this enzyme system.