Tilapia BioFloc PDF

TILAPIA PRODUCTION IN
BIOFLOC SYSTEMS
YORAM AVNIMELECH
TECNION, ISRAEL INST. Of TECHNOLOGY
agyoram@technion.ac.il
Tilapia production, a growing industry
Tilapia is becoming a very popular fish

and its production an important
aquaculture
industry
Tilapia, an ideal fish for Biofloc Technology
Grow well in dense

cultures
Resistant •
FILTER FEEDER •
Very few studies as •
compared with
shrimp
Fish Biomass
Normally, 20-30 kg/m2
10 times higher than shrimp BFT ponds!
Implications:
 High Biomass 20-30 kg/m3
 High feeding (ca 500 g feed/m3 per day!)
 Very high microbial activity
 High floc volume (20-50 ml/l).
 Very high natural feed storage.
 High levels of feed residues
 Need to drain out daily (or twice daily) excessive
sludge.
 Pond constructed to facilitate sludge draining
and perfect mixing.
 High and effective aeration: 10-20 hp/1000 m2
pond
Drain bottom sludge twice daily till you
get clear water
Bioflocs and Floc volume
 Bio flocs are made of bacteria, protozoa,

feed residues etc.
 Bioflocs size may reach
A few mm diameter
 Effectively harvested
by tilapia
200 µm x10 x20
200 µm 200 µm
x10 x10
Bio flocs are made of bacteria,

F
l
protozoa, etc. Typicaly their
o
diameter is 0.1-2 mm.

Protein Recycling
 Normally, fish or shrimp recover just ~25% of

feed protein.
 In bacterial controlled ponds, they eat the
protein twice; Once in the feed and then they
consume microbial protein. The protein
recovery reaches almost 50%.
 Protein is the most expensive part of the feed.

Expt. # 1
control BFT
51 days
FEED C/N 11.1 16.6
Daily Gain (%) 1.59a 2.0b

FCR 2.62 2.17
PCR 4.38 2.42
FEED COST (US$/Kg fish) 0.848 0.583
Exp. # 2 (30 days)
C/N 11.1 16.6

Daily gain (%) 1.63a 2.22b
FCR 2.62 2.02
PCR 4.35 2.18
Feed cost 0.848 0.543
(US$/Kg fish)
Preparing an 15N tagged bioflocs:
 1. Prepare large enough batch of biofloc

suspension (water + feed, mix & aerate).
 2. Add 15N salt.
 3. Add starch, at a C/N ratio of > 15
 4. After a few hours, practically all 15N is in
the bioflocs.
Results 1: % 15N in fish
% 15 N in fish
0.5500
0.5000
0.4500
% 15N
0.4000
0.3500
0.3000
1 3 5 7 9 11 13 15
Days
Residence time of bioflocs
 Bioflocs were taken up by fish and degraded

biologically. Yet, the amount of bioflocs
stayed almost constant. This implies that new
flocs are constantly produced (using the
excreted N).
 The residence time of bioflocs was
calculated to be around 8 hours. The flocs
seem to be a very dynamic system.
 most cells in the flocs are young and active.
Data on feed protein utilization
 Conventional fish, shrimp ponds 20-25%

 BFT Tilapia ponds (Avnimelech) 45%
 BFT Shrimp ponds (McIntosh) 45%
 Closed shrimp tanks (Velasco) 63%
 BFT shrimp ponds, 15N study 18-29% of total N

consumption (Michele Burford et al.)
* Tilapia, 15N Study, flocs supplied about 50% of
fish protein requirement. (Avnimelech).
Feed Utilization
 Fish or shrimp growing in BFT systems

eat the pellets when applied, but eat
bioflocs all the time.
 Confirmed in a work done by Albert Tacon
with shrimp.
 Observations with tilapia.
 Effects on lowering of FCR in tilapia
production
Fresh results from the field:
 Tilapia fingerlings (120 g) stocked to over-

wintering facility on January 4, 2011.
 Feeding ~ 1% BW
 On February 18, they weighed 171 g
 Daily growth of 1.16 g/fish
 FCR = 0.5
OVER-WINTERING
Results
Temperature and oxygen control 

Dissolved oxygen: 9 – 10 mg O2/L –
Average temperature: 18 2 C –
EFFECT OF WATER EXCHANGE RATE ON PRIMARY
AND SECONDARY INFECTION OF FISH BY STREPTOCOCCUS
Treatment Dead fish Sick fish Total infected
Control 9 (9) 2 (2) 11

Control
a)Injected
fish BFT BFT 12 (3) 4 (2) 16
High 7 (5) 4 (1.5) 11 (4.7)

a)Non- Control
exchange
injected
fish ASP 1.8 1.3 (1.0)
BFT 3 (1.4)
(1.7)
High water exchange = 0.5l/min per kg fish (700% per day)
BFT< Limited water exchange = 10% per day
Conclusions
 Biofloc technology is especially adapted to raise

tilapia production up to 20-30 kg/m2.
 This can be done using not too expensive system.
 BFT enables feed recycling, high feed quality and
reduced expenses.
 BFT reduces disease.
 The system is friendly and forgiving.
 More research is needed
Thank You
Yoram

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TILAPIA PRODUCTION IN

TECNION, ISRAEL INST. Of TECHNOLOGY

Tilapia is becoming a very popular fish

Grow well in dense

 Bio flocs are made of bacteria, protozoa,

Bio flocs are made of bacteria,

diameter is 0.1-2 mm.

 Normally, fish or shrimp recover just ~25% of

 Protein is the most expensive part of the feed.

FEED C/N 11.1 16.6

Daily Gain (%) 1.59a 2.0b

C/N 11.1 16.6

 1. Prepare large enough batch of biofloc

 Bioflocs were taken up by fish and degraded

 Conventional fish, shrimp ponds 20-25%

 BFT shrimp ponds, 15N study 18-29% of total N

 Fish or shrimp growing in BFT systems

 Tilapia fingerlings (120 g) stocked to over-

Temperature and oxygen control 

Control 9 (9) 2 (2) 11

High 7 (5) 4 (1.5) 11 (4.7)

 Biofloc technology is especially adapted to raise

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