PRODUCTION OF

STARCH-BASED
BIOPLASTIC USING
VIGNA RADIATA
(MUNG BEANS)
 A. RATIONALE C. EXPECTED RESULTS

In the Philippines, most Filipinos are fond of The mung bean starch will make an effective
utilizing single-use plastics and buying products in bioplastic. According to the studies gathered, starch
sachets instead of the ones in containers. Also, the from banana, potato and corn starch was a potent
practice of using plastics in most of the grocery stores variable in making bioplastic. The product might not
and wet markets contributes to the upheaval of plastic be as chemically-complex as the normal, synthesized
waste. Using plastics is preferred by people since plastic the people use today be less harmful and more
they contribute to our health, safety, and peace of environmental-friendly.
mind. The phenomenal rise in the usage of plastics is
due to their low cost and better properties which D. MATERIALS AND METHODS
include flexibility, rigidness, brittleness, and ability to i. Experimental Design
be moulded into a variety of shapes and lightness
(Stevens, 2002). To minimize the harmful effects of
ordinary plastics, the production of bioplastics took Milling the Obtaining the
Preparation of
place. Its decomposition takes months, faster than the Mung beans beans pure starch
known regular plastics. They are made from
renewable substances that are environment-friendly
such as starches, food leftovers and will later be
synthesized into organic wastes. Production of
Diluting the mung Adding the
starch-based
bean glycerol
plastic
Vigna radiata, which is also known as mung
beans, can be found almost everywhere in the
community. It is one of the most cultivated crops in
ii. Data collections and Proecdures
tropical and subtropical regions. Mung beans also
- Preparation of mung beans
contain carbohydrates which can be easily digested
Seeds should be gathered
compared to other bean crops. Its starch content has
and softened by soaking them
a great potential in making bioplastics.
in distilled water overnight. The
As stated in a study, crops had to be diverted seeds will then be husked.
for the production of bioplastics, but in the last decade
or so, there has been a focus on using waste - Milling the beans
materials (such as banana peels, potato peelings, etc) Seeds should pass under
to produce bioplastics instead. By utilizing waste a food mill to make sure they
products, use of scarce resources can be maximized. are well ground.
(Creative Mechanisms Blog, 2017). According to a
study, starch contained in Vigna radiata was 31.1% - Obtaining the pure starch
on a whole-seed basis. The shape of the starch The starch will be collected
granule was oval to round to bean-shaped, with after milling the solution.
granules 7–26 μm in diameter. The total amylose
- Preparation of starch-based
content was 45.3%, of which 12.1% was complexed bioplastic
by native lipids (Hoover et al., 1997, p. 401-408). Dilute 15g of mung bean
starch with 150ml distilled
B. OBJECTIVES water in a 500ml beaker.

This study generally aims to scrutinize the Stir at 2 r.p.m. using a

potential of producing bioplastic using Vigna radiata magnetic stirrer. Pipette 18ml
(mung bean) starch. of 0.1M HCl in the mixture and
add the same amount of 0.1M
Specifically, it aims to: (1) produce a NaOH for neutralization.
bioplastic based on Vigna radiata starch; (2) compare
Add 12ml of 1% glycerol
the properties of the said bioplastic with the traditional and switch the heater to
plastics; (3) investigate the feasibility of using mung 100oC. Allow the mixture to
beans as bioplastic; (4) check the morphological heat for about 15 mins.
changes of the bioplastic.
 The mixture will form an Potential Health Effects
opaque gel after an hour. Eye: May cause eye irritation. Skin:
Spread the gel on a mold of 2 May cause skin irritation. Low
mm thickness. Allow the hazard for usual industrial handling.
sample to dry.
Ingestion: Ingestion of large
iii. Data analysis amounts may cause gastrointestinal
irritation. Low hazard for usual
- Tensile test industrial handling. May cause
The plastic will be placed headache.
between two “grips” which will
clamp the material. This grip Inhalation: Low hazard for usual
will pull the plastic at both end. industrial handling. Inhalation of a
One end is fixed, and one end mist of this material may cause
will be adding weight. The respiratory tract irritation.
weight will be increased while
at the same time, the measure First Aid Measures
of the change in length will be Eyes: Flush eyes with plenty of
recorded. water for at least 15 minutes,
occasionally lifting the upper and
- Degradation test lower eyelids. If irritation develops,
Two 40 mL beakers and 1.1 get medical aid.
grams of preweighed piece of
bioplastics were taken, the Skin: Flush skin with plenty of water
preweighed material was for at least 15 minutes while
placed under the beaker removing contaminated clothing
containing soil with a depth of 5 and shoes. Get medical aid if
cm. A small amount of water irritation develops or persists. Wash
will be sprinked on to the soil clothing before reuse.
so that the bacterial activities
would be enriched. The Ingestion: Never give anything by
samples will be kept in the mouth to an unconscious person.
beaker for at least 15 days and Do NOT induce vomiting. If
will be checked regularly. conscious and alert, rinse mouth
and drink 2-4 cupfuls of milk or
- Swelling test water. Get medical aid if irritation or
A preweighed piece of samples symptoms occur.
were prepared were taken in
the test tube to check the Inhalation: Remove from exposure
protuberance and other and move to fresh air immediately.
morphological changes, it was If not breathing, give artificial
carried out on the medium respiration. If breathing is difficult,
containing various solvents give oxygen. Get medical aid if
such as water, ethanol and cough or other symptoms appear.
methanol medium. Where the
II. ETHANOL
deliberated samples were kept
in the medium for about 2
Dangers
hours and the results were
- Harmful if swallowed
recorded accordingly.
- Acute Toxic if inhaled
- Data Analysis - Causes skin irritation
- Causes serious eye irritation
The results of the tensile
- Suspected of causing cancer
experimentation, the degradation
- Causes damage to organs
testing, and the tensile test will be
(liver, kidneys) through
analysed and will be compared
prolonged or repeated
with a normal, synthesized plastic.
exposure (Inhalation, oral)
E. RISK AND SAFETY First Aid Measures
- Check the vital functions.
I. GLYCEROL Unconscious: maintain
adequate airway and
respiration.
 Respiratory arrest: artificial - Causes damage to organs
respiration or oxygen. (liver, kidneys, central
nervous system, optic
Cardiac arrest: perform nerve) (Dermal, oral)
resuscitation.
Precautionary statements
Victim conscious with labored - Keep away from heat,
breathing: half-seated. Victim in sparks, open flames, hot
shock: on his back with legs surfaces.
slightly raised. - No smoking.
- Keep container tightly
Vomiting: prevent closed.
asphyxia/aspiration - Ground/bond container
pneumonia. Prevent cooling by and receiving equipment.
covering the victim (no - Use explosion-proof
warming up). Keep watching electrical, ventilating,
the victim. Give psychological lighting equipment
aid. Keep the victim calm, - Use only non-sparking
avoid physical strain. tools.
Depending on the victim's - Take precautionary
condition: doctor/hospital. measures against static
Never give alcohol to drink. discharge.
- Do not breathe mist,
Inhalation: Remove the victim vapors, spray.
into fresh air. Respiratory - Wash exposed skin
problems: consult a thoroughly after handling.
doctor/medical service. - Do not eat, drink or smoke
when using this product.
Skin: Wash immediately with - Use only outdoors or in a
lots of water. Soap may be well-ventilated area.
used. Do not apply (chemical)
neutralizing agents. Take victim F. SUCCESS CALENDAR
to a doctor if irritation persists.
ACTIVITY PLANNED EXPECTED DATE
Eyes: Rinse immediately with OF COMPLETION
DATE
plenty of water. Do not apply
Choosing Topic Completed
neutralizing agents. Take victim
to an ophthalmologist if Collecting Completed
irritation persists. background
information
Ingestion: Rinse mouth with Problem and Completed
water. Immediately after Hypothesis
ingestion: give lots of water to Design for Completed
drink. Do not give milk/oil to experiment
drink. Do not induce vomiting. Getting First week of First week of
Call Poison Information Centre materials ready August August
(www.big.be/antigif.htm). Making data Second week of Second week of
Consult a doctor/medical table August August
service if you feel unwell. Recording the Second week of Third week of
Ingestion of large quantities: data table August August
immediately to hospital. Take Stating results Third week of Third – fourth
the container/vomit to the August week of August
doctor/hospital. Drawing Third week of Third – fourth
conclusions August week of August
III. METHANOL Compiling Fourth week of Fourth week of
bibliography August – First August – First
Dangers week of week of
- Highly flammable liquid September September
and vapour Making the Fourth week of Fourth week of
- Toxic if swallowed, in research paper August – First August – First
contact with skin or if week of week of
inhaled September September
G. REFERENCES Rahman,A. & Miller,C.D. (2017). Microalgae
as a source of bioplastics. Algal Green
Creative Mechanisms Blog (2017, January Chemistry, 121-138. Retrieved from
22). Everything you need to know about PHA https://doi.org/10.1016/B978-0-444-63784-
[Blog post]. Retrieved from 0.00006-0
https://www.creativemechanisms.com/blog/e
verything-you-need-to-know-about-pha- Soomaree, K. (2016). Production of Potato
polyhydroxyalkanoates Starch based Bioplastic. Retrieved
fromhttps://www.researchgate.net/publication
DeLorenzo, S. (2011, May 06). How to /306263110_Production_of_potato_starch_b
Extract Starch From Mung Bean. Retrieved ased_bioplastic
from
https://www.ehow.com/how_8369059_extrac Wikihow. (2019, April 09). How to Make
t-starch-mung-bean.html Bioplastic. Retrieved from
https://www.wikihow.com/Make-Bioplastic
Extracting Starch from Wheat [Video file].
Retrieved Yaradoddi, J., Patil, V., Ganachari, S.,
from https://www.youtube.com/watch?v=Ne Banapurmath, N., Hunashyal,A., Shettar, A.
DgBuNoOAg (2016). Biodegradable Plastic Production
from Fruit Waste Material and its Sustainable
Hoover R., Li Y.X., Hynes G., Senanayake Use for Green Applications. International
N.(1977). Physicochemical characterization Journal of Pharmaceutical Research & Allied
of mung bean starch. Food Sciences. 5(4), 56-66.
Hydrocolloids.11(4), 401-408. Retrieved from
https://doi.org/10.1016/S0268-
005X(97)80037-9 H. PROJECT COST AND RESOURCES
EQUIPMEN SUPPLIER RESOURCE COST
Keshav, S.(2015-2016). Production of T S S
INSTITUION
bioplastics. DOI: S
10.13140/RG.2.2.24339.27682 Mung - Local P160 (2 kg)
beans market
Keshavarz, T. and Roy, I. 2010. Distilled - Local P80
Polyhydroxyalkanoates: bioplastics with a water market
green agenda. Current Opinion in Glycerol Philippine - P35 (1
Microbiology. 13 (3), pp. 321-326. Medical bottle)
doi:10.1016/j.mib.2010.02.006 Supplies
Ethanol - Alysons’ Approximate
Chemical ly P1,000 –
Keziah, S., Gayathri, R., and Priya, V.
Enterprises 10,000
(2018). Biodegradable plastic production , Inc.
from Cornstarch. Retrieved from Methanol - Alysons’ Approximate
http://jprsolutions.info/files/final-file- Chemical ly P1,000 –
5b482d6142ebe4.80504835.pdf Enterprises P10,000
, Inc.
Manimaran, D.S., Nadaraja, K.J., Vellu, J.P., TOTAL Approximate
Francisco, V., Kanesen, K., Bin Yusoff, Z. ly P2,275 –
(2016). Production of Biodegradable Plastic P22, 275
from Banana Peel. Journal of Petrochemical
Engineering Department., 1(1), 2.

Michigan Technological University (n.d.)

Tensile test experiment. Retrieved from
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