Electrochemical Microbial Biosensor: Presented By-Ruchi M.SC (F)
Electrochemical Microbial Biosensor: Presented By-Ruchi M.SC (F)
Electrochemical Microbial Biosensor: Presented By-Ruchi M.SC (F)
Biosensor
Presented by-
Ruchi
M.Sc (F)
Biosensor
• Biosensor basically consists of transducer in
conjunction with a biologically active molecule
that converts a biochemical signal into a
quantifiable electric response.
• Some of the major attributes of a good biosensing
system are its repeatability, reproducibility,
specificity, sensitivity, reliability, portability, ability
to function even in opaque solution, good
response time and simplicity in operation.
Microbial biosensor
• A microbial biosensor consists of a transducer in
conjunction with immobilized viable or non-viable
microbial cells.
• Viable cells make use of respiratory and metabolic
functions of the cell, analyte can be a substrate or
inhibitor of these processes. Ex. BOD biosensor.
• Non viable cells obtained after permeabilization
or whole cells containing periplasmic enzymes
have mostly been used as an economical
substitute for enzymes.
ELECTROCHEMICAL MICROBIAL
BIOSENSOR
• It measures electrical signal, which is generated during
the biochemical interaction between the biologically
active part and substrate.
• It generally consists of a working electrode, a
transducer layer for detection (microorganism) and
recording equipment.
• Signal from the transducer, produced by the
electrochemical reaction is recorded and correlated
with the concentration and correlation with the
concentration and composition of the compound
present and displayed as an electrical expression.
Types of electrochemical biosensor
• Amperometric biosensor- involves measuring
the current generated by electrochemical
oxidation or reduction of electroactive species
at a constant applied potential.
• Potentiometric biosensor- measures the
electric potential difference between a
working and reference electrode.
Types of Electrochemical biosensor
• Conductometric biosensor- measures changes
in the conductance of the system due to
presence of the analyte. Ex - urea biosensors,
with immobilized Brevibacterium
ammoniagenes very successfully used during
dialysis and renal surgery.
• Voltametric biosensor- they record and
correlate each electric signal (electric current
and potential difference) with a corresponding
sample.biosensor using Circinella sp. for
detection of Copper ions.
Amperometric biosensor for the detection of
glucose
• Biosensor was constructed using
Aspergillus niger cells.
• These microbes contain glucose
oxidase enzyme (GOx).
• GOx catalyzes the oxidation of
glucose to gluconic acid
β-D-Glucose + FAD - Gluconic
acid + FADH₂
• FAD is known as the redox center of
GOx. For reoxidation of FADH₂
various electron mediator are used
FADH₂ + Mₒₓ - FAD + Mred
• Oxygen is a natural electron GLUCOSE BIOSENSOR
acceptor for GOx has been
commonly used as mediator
FADH₂ + O₂ - FAD + H₂O₂
Amount of gluocse can be measyred by measuring
the consumption of hydrogen peroxide.
hydrogen peroxide is oxidized at platinum anode.
the electrode easily recognizes the number of
electron transfers, and this electron flow is
proportional to the number of glucose molecules
present in blood.
H2O2 2H O2 2e
used for measurement of blood glucose levels
fpr screening, diagnosis
and management of
diabetes.
Glucose biosensor
• The working potential for this type of
microbial biosensor is around 0.7V. However
many substance are able to be reduced at this
potential, resulting in interference.
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