Scribd
Upload
41 views

Model Questions For The Sessional Exam I - 2019

The document contains 12 multiple choice questions related to techniques used for nucleic acid extraction and analysis. Specifically, it asks about reagents used for concentrating nucleic acids, staining of RNA separated by gel electrophoresis, fractionation of bacterial RNA on a density gradient, alternatives when the preferred staining dye is unavailable, definitions related to cell wall disruption, identification of the correct reaction for RNA denaturation using a reagent, parameters to monitor during phenol equilibration, molecules that form complexes with CTAB, best methods for bacterial protein extraction, effect of salt concentration on nucleic acid solubility, and impact of adding VRC as an RNase inhibitor during phenol extraction of RNA.

Uploaded by

Sayan Mallick
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
41 views

Model Questions For The Sessional Exam I - 2019

The document contains 12 multiple choice questions related to techniques used for nucleic acid extraction and analysis. Specifically, it asks about reagents used for concentrating nucleic acids, staining of RNA separated by gel electrophoresis, fractionation of bacterial RNA on a density gradient, alternatives when the preferred staining dye is unavailable, definitions related to cell wall disruption, identification of the correct reaction for RNA denaturation using a reagent, parameters to monitor during phenol equilibration, molecules that form complexes with CTAB, best methods for bacterial protein extraction, effect of salt concentration on nucleic acid solubility, and impact of adding VRC as an RNase inhibitor during phenol extraction of RNA.

Uploaded by

Sayan Mallick
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 2

Model Questions for the Sessional Exam I - 2019

1. Which one of the following reagents can be used for the concentration of DNA/RNA
solution?
A. 2-butanol B. 1-butanol

C. water-saturated 2-butanol D. water-saturated 1-butanol

2. Why acridine orange (AO), but not ethidium bromide (EtBr), needs to be used for staining
RNA fractionated using glyoxal as the denaturant?

A. Glyoxal destroys spectral properties of EtBr

B. EtBr cannot bind to glyoxal-RNA adduct

C. AO can bind RNA even in the presence of glyoxal

D. AO fluoresces much better than EtBr when bound to glyoxal-RNA adduct

3. You have layered total RNA preparation (mixture of mRNA, rRNA, and tRNA) from
bacterial cells on a CsCl equilibrium density gradient of density range of 1.2 – 1.7 gm/cc. What
will be the order of fractionation from the lowest density to the highest density?

A. mRNAs > > 5S rRNA > 16S rRNA > 23S rRNA > tRNAs

B. tRNAs > 5S rRNA > 16S rRNA > 23S rRNA > mRNAs

C. 5S rRNA > 16S rRNA > 23S rRNA > tRNAs > mRNAs

D. All the mRNAs, rRNAs, and tRNAs will be at the bottom of the gradient

5. You carried out electrophoretic fractionation of total RNA using glyoxal as the denaturant.
Since glyoxal destroys spectral properties of ethidium bromide (EtBr), you need to use acridine
orange (AO) to stain the RNA after electrophoresis! Before starting the electrophoresis, you
forgot to check whether AO is available for staining! Unfortunately, you could not get AO!
Which one of the following options is the sensible way out?

A. Reverse glyoxal adduct and stain with EtBr B. Stain with methylene blue

C. Use higher quantity of EtBr and stain the RNA

D. Discard the gel and do the experiment fresh with formaldehyde agarose gel

6. The terms, 'spheroplast' and 'protoplast', are applicable to:

A. Gram-negative bacterial cells B. plant cells

C. yeast cells D. Gram-positive bacterial cells

1
7. From the equations given below, identify the correct equation for the denaturation of RNA
using methyl mercuric hydroxide. In the formula, B-NH or B-NH2 or B-N, B stands for the
base portion and the -NH, -NH2, or -N stands for reactive group on the base.

A. CH3-Hg-OH + HN-B CH3-Hg-N-B + HO-H

B. CH3-Hg-OH + HN-B CH3-Hg-H-HN-B + HO-

C. CH3-Hg-OH + HN-B CH3-Hg-HN-B + HO-

D. CH3-Hg-OH + HN-B CH3-Hg-HN-B + HO-H

8. When you equilibrate phenol with a buffer, what parameter you should monitor as the
indication for the completion of the equilibration process?

A. phenol should no more be able to remain as solid at room temperature

B. pH of the phenol phase should become same as that of the buffer phase

C. phenol phase should not be able to take aqueous phase any more

D. pH of the buffer phase should become same as that of the phenol phase

9. With which of the following molecules does CTAB form the CTA salt of the molecule?

A. Nucleic acids only B. DNA and polysaccharides

C. Nucleic acids and negatively charged polysaccharides D. Polysaccharides only

10. The best method to prepare protein from large quantity of bacterial cells is:

A. Sonication B. Grinding with glass powder

C. Freezing/thawing D. French Press

11. Which one of the following nucleic acid molecules have maximum solubility in a salt
solution of high molarity?

A. 3 kbp CCC plasmid DNA B. single-stranded DNA with stems & loops

C. 23S ribosomal RNA D. mRNA with secondary structure

12. What will happen if VRC is used in the buffer during phenol extraction of total RNA?

A. VRC is soluble in phenol and hence will not be available as RNase inhibitor

B. VRC forms bonds with the nucleosides in the RNA in the presence of phenol

C. VRC gets inactivated by phenol and hence not useful as an RNase inhibitor

D. VRC binding to RNA will block deproteinisation action of phenol on mRNPs

You might also like

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy