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Andat 4

The document describes procedures to analyze the composition of milk through various tests. It details calculations to determine the density of pure and diluted milk. Various chemical reactions are described to identify key milk components like casein, proteins, fats, lactose, calcium, and phosphates. Casein is shown to precipitate out of solution through reactions with calcium and acetic acid. Reactions also detect proteins through color changes indicating amino acid residues and peptide bonds.

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32 views

Andat 4

The document describes procedures to analyze the composition of milk through various tests. It details calculations to determine the density of pure and diluted milk. Various chemical reactions are described to identify key milk components like casein, proteins, fats, lactose, calcium, and phosphates. Casein is shown to precipitate out of solution through reactions with calcium and acetic acid. Reactions also detect proteins through color changes indicating amino acid residues and peptide bonds.

Uploaded by

putri
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOC, PDF, TXT or read online on Scribd
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A.

ANALISIS DATA
1. Perhitungan
a. Penetapan Berat Jenis
 Berat Jenis Air Susu Murni (Bear Brand)
Diketahui : Volume air susu murni = 10 mL
Massa erlenmeyer kosong = 61,13 gram
Massa erlenmeyer + susu murni = 71, 28 gram
Ditanya : Massa jenis susu murni = ..?
Penyelesaian :

Massa air susu murni = (massa erlenmeyer + susumurni) – massa erlemeyer


kosong

= 71,28 – 61,23 gram


= 10,15 gram
Massa jenis susu murni =

= 1,015 gram/mL
 Berat Jenis Air Susu Murni (Bear Brand) yang Diencerkan
Diketahui : Volume air susu encer = 10 mL

Massa erlenmeyer kosong = 76,22 gram

Massa erlenmeyer + susu encer = 86,23 gram

Ditanya : Massa jenis susu encer = ..?


Penyelesaian :
Massa air susu encer = (massa erlenmeyer + susu encer) - massa erlemeyer kosong
= 86,23 – 76,22 gram
= 10,01 gram

Massa jenis susu encer =

= 1,001 gram/mL
 Berat Jenis Filrat Air Susu dari Pengendapan Kasein
Diketahui : Volum filtrat air susu = 10 mL

Massa erlenmeyer kosong = 61,13 gram

Massa erlenmeyer + filtrat air susu = 70,229 gram

Ditanya : Massa jenis filtrat air susu = ...?

Penyelesaian :

Massa filtrat air susu = (massa erlenmeyer + filtrateair susu )-massa erlemeyer
kosong

= 70,229 – 61,13 gram

= 9,099 gram

Massa jenis filtrat air susu =

= 0,9099 gram/mL
b. Reaksi Air Susu
 Susu Bear Brand
pH awal =7
pH setelah 1 jam =6

2. Persamaan Reaksi
a. Pengendapan Kasein
Susu(l) + H2O(l) → kasein(s)
[Ca2+] [kaseinat2-](s) + 2CH3COOHCa(CH3COO)2 + kasein(s)
b. Reaksi-Reaksi Warna Protein
 Reaksi Biuret (Untuk Ikatan Peptida)
 Reaksi Millon-Nasse (untuk Tirosin)

 Reaksi Hopkins-Cole (Untuk Triptofan)

 Reaksi Xanthoprotein (Untuk Asam Amino dengan Inti Benzena)


 Reaksi Uji Sulfur

 Reaksi Molisch
c. Reaksi Newman untuk Kasein (P-organik dari Kasein)

d. Grease Spot Test (Test Noda Lemak)


1. Endapan kasein + Eter Kasein tidak larut dalam eter
2. Terdapat tetesan bening, kertas menjadi transparan yang menandakan ada lemak
e. Menunjukkan Adanya Laktalbumin
 Susu Bear Brand
Filtrat kasein (pH 5) (+) terdapat koagulan putih

f. Menunjukkan Adanya Laktosa


 Reaksi Benedict

 Reaksi Fehling

g. Menunjukkan adanya Ion Ca dan P-anorganik


Fitrat (no 7) + NH4OH endapan putih gelartinous dari Ca dan Mg fosfat
Endapan + K-oksalat larutan berwarna putih keruh

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