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Chemistry Project

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AIM

Effect of heat on vitamin C in tomatoes

Abstract

The Objective : Vitamin C is an important vitamin,


and tomatoes are a good source of Vitamin C. Most
tomato products we consume have been processed
by heat, and I wanted to discover if cooking altered
the level of Vitamin C.

My goal was to determine if heating the tomatoes to


three different temperatures affects the level of
Vitamin C. My hypothesis is that the Vitamin C
content of tomatoes will decrease when heated
because Vitamin C is water soluble and is affected
by heat.
Methods/Materials
I used titration to test my hypothesis. The Vitamin
C in the tomatoes is the titrant, and iodine is the
titrating solution. I made a tomato solution by
blending store-bought red tomatoes with 200mL of
distilled water.

I filtered the solution to remove seeds. A 10mL


sample was removed and set aside as the control.

The Vitamin C from this sample is the dependent


variable. The remaining tomato solution was heated
on a gas stove, and three 10mL samples were taken
at three different temperatures, 50C, 75C, and 98C
(independent variable).
While the samples cooled to a standard temperature
of 17C, I prepared a starch solution (1T cornstarch
and 200mL distilled water).

S
Ten drops of the starch solution were added to each
of the four samples.

I then added the titrating solution, iodine (Iodine


Tincture USP) one drop at a time to the samples and
recorded the number of drops necessary to change
the pink colored tomato/starch solution to a
blue/black color.

The results were charted, and compared on a graph.


I repeated this procedure a total of three times.
TRAIL TEMPERATURE AMT OF
NO IODINE
REQUIRED
1
2
3

Results
The presence of Vitamin C in a fresh tomato
solution declined after it was heated.
In the first trial, my uncooked sample needed five
drops of iodine to change color, while the other
three samples needed 20% less solution (four
drops).
Trial Two started with six drops for the Control and
concluded with four drops for the 98C sample.
The final trial gave the clearest results--the Control
required seven iodine drops to change to blue/black
and the 98C sample only required four drops (43%
less).

Conclusions/Discussion
This experiment showed that Vitamin C in a tomato
can decrease due to heat. Heat causes the Vitamin
C content in tomatoes to decrease by decomposing
the water-soluble vitamin. Tomatoes that are
cooked will have less Vitamin C than raw tomatoes.
If you want the most Vitamin C from a tomato--
EAT FRESH!
This project will be investigating that Vitamin C in
raw red tomatoes is reduced by heat.

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