Forensic Research Paper
Forensic Research Paper
Forensic Research Paper
Forensic Science j
Submitted To:
j Submitted By: j
PRN: 16010125210
j
Chapter 1 – PRELIMINARY
j j j jjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjjj j 4-5
1.1 INTRODUCTION
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1.2 KEYWORDS
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1.3 SCOPE
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2.3 HYPOTHESIS
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2.3 LIMITATIONS
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Chapter 3 – ANALYSIS
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3.1 REASONS FOR NON-DETECTION OF POISON IN THE VISCERA
J J J J J J J J
Chapter 4 – SUMMARY
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4.1 RECOMMENDATIONS AND SUGGESTIONS
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4.2 CONCLUSION
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Shashank Upadhyay* j
Abstract j
Thebconclusion of cause of deathbin a case wherebthe death is due to poisoningbbut viscera report
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j gives negative result, poses confusion to the autopsy doctor, law and public. The intricacy of failure
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j to find poisoniin viscera of the individual whose death is due to poisoning is a routine problem in
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j India, the cause of which has been analysed in this paper.iAnother issue is that only common
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j poisons are tested in a viscera report. The residual analysis of poisons is limited to 10-15 poisons
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j commonly available in the area. The other major killer poisons/chemicals like insulin, KCl,
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j Adrenaline can’t be detected in viscera. The salient points covering various aspects of viscera
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j analysis in various Forensic Science Laboratories have been discussed with special reference to
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j the false positive or false negative results and interpretation of viscera report when it is negative in
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j truly positive cases and vice-versa. Judgments have been referred to for a better understanding of
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j this issue and the question of whether a negative viscera report implies that the cause of death was
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______________________________________________________________________________
*4th Year Law Student, Symbiosis Law School, Pune, India.
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Email: shashank.upadhyay@symlaw.ac.in
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3
CHAPTER 1: PRELIMINARY J J
1.1 INTRODUCTIONJ J
• Clinical records2 j
• Postmortem examination3 j
• Chemical analysis4 j
When a poisoning death case is presented to an autopsy surgeon, the doctor is required to preserve
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j the viscera for chemical analysis to know the nature of poisoning. The facility for the same is being
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j provided by the authorities in the State or Central Forensic Science laboratories (hereinafter
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j referred to as “FSL”). The viscera boxes are submitted in FSLs on a priority basis depending
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j upon the gravity of the case. The report comes from FSL and the same is being read as such by
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5
j Section 293 CrPC. The role of the autopsy surgeon is generally limited to preservation of viscera
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j and the cause of death is commented upon only after the receipt of chemical analysis report. When
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j the report clearly detects a poison, the cause of death may be easily opined by the autopsy surgeon.
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j In many cases the viscera report comes negative, or detects the poison in the quantity not sufficient
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j to cause death. This puts the autopsy surgeon in a fix as the police expect him to give cause of death
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j so as to reach a logical ending to the investigation. When there is no allegation in the case, the
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j poisoning being either accidental or suicidal, and no charges under Indian Penal Code are being
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j made out after the investigation, the case is closed by the investigating agency/police even in the
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j presence of negative viscera. The problem starts when there is suspicion of foul play in the case.
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j This generally comes in the cases of murder,6 dowry deaths,7 rash and negligent act while handling
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j the poison,8 and abetment of suicide.9 In these types of cases after the negative viscera report, when
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1
K Matiharan & AK Patnaik, Modi’s Medical jurisprudence and Toxicology (5th ed. Lexis Nexis, 2010).
2
CK Parikh, Textbook of Medical Forensic Medicine and Toxicology (6th ed. CBS Publishers, 1999).
3
KSN Reddy, The essentials of Forensic Medicine and Toxicology (29th ed. Sugana Devi Publishers, 2018).
4
K Vij., Textbook of Forensic Medicine and Toxicology: Principles and Practice (5th ed. Elsevier Publishers, 2011).
5
The Code of Criminal Procedure, 1973 (Act No. 2 of 1974).
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6
Indian Penal Code, 1860 § 302 (Act No. 45 of 1860).
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7
Indian Penal Code, 1860 § 304B (Act No. 45 of 1860).
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8
Indian Penal Code, 1860 § 304A (Act No. 45 of 1860).
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9
Indian Penal Code, 1860 §306, (Act No. 45 of 1860).
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4
j the autopsy surgeon terms the death as unnatural and due to poisoning, his opinion is frequently
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1.2 KEYWORDS J
j Report. j
1.3 SCOPE J
This paper reviews the current knowledge of the post-mortem toxicology when the death has been
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j caused due to poisoning but the same does not show in the viscera report. There have been various
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j false negative cases in India wherein the cause of death is unable to be identified because a viscera
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j report is only able to determine common poisons and not other major killer poisons and chemicals.
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j Because of this, the court does not have enough evidence to convict the accused and justice is
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j unable to be served in a fair manner. The poisons detected by the various FSLs in India were
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• CFSL (Kolkata): Metallic poisons, ethyl alcohol, methyl alcohol, cyanide, phosphides,
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• FSL (Delhi): Metallic poisons, ethyl alcohol, methyl alcohol, cyanide, phosphides, alkaloids,
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j alcohol, phosphides. j j
It can thus be clearly seen that generally the viscera reports were found to be positive only for ethyl
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j alcohol, methyl alcohol, phosphides, zinc Phosphide, and aluminium phosphide. This research
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j paper discusses in detail the problem of False Negative cases emphasizing on the methods used by
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j FSLs, present problems in detecting specific poisons, past studies and Court Judgments. It also
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j recommends and suggests some changes, which could perhaps be the apt solution for curbing the
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10
Dr. Abhishekh Yadav, Death due to Poisoning: Intricacies Thereof, 62 The Indian Police Journal 1 (2016).
5
j false negative cases in courts and assisting the medical professionals in accurately determining the
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The authors have adopted a secondary methodology of research using articles, journals and books.
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j This has been a doctrinal and analytical research, mostly based on publications of renowned
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j authors. It is the author’s assurance that no part of this research paper is plagiarised.
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This paper aims to analyse the current scenario of post mortem toxicology with respect to a negative
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j viscera report. The issue that shall be dealt with by way of this research paper:
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2.2.1 Whether a negative Viscera report can be used to completely rule out poisoning as the
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j cause of death. j j
2.3 HYPOTHESIS
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It is hypothesised that when a viscera report shows a negative result, ‘poison’ is ruled out as the
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2.4 LIMITATIONS
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The limitation of this research paper is that it is based only on secondary sources, which is not
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j considered as reliable as primary research. Another limitation is that this research paper is confined
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j to the Indian jurisdiction and therefore unable to cover all aspects of toxicological analysis in case
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6
CHAPTER 3: ANALYSIS J J
After a thorough analysis from renowned journals, articles and books, it was discovered that there
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j are some instances in which poison is not detected in the viscera report; the reasons thereof have
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j been discussed in this paper. It was also found out that there are some poisons, which cannot be
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j detected by a viscera report at all. Lastly, Supreme Court judgments and recent decisions are
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The procedure followed in Indian laboratories is of residual analysis of the poisons meaning the
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j actual poisons in their original chemical formre detected and not the metabolites.11
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It is well known that usual routine toxicological screening procedures may not detect hemoglobin
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If the specific tests are not performed, some poison may be missed in conventional screening
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j procedures by FSL experts, like Insulin, vegetable poisons, organic poison especially alkaloid and
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The poison has been eliminated from the body due to vomiting, purging. Gaseous or volatile
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11
AK Jaiswal & Millo, Handbook of Forensic Analytical Toxicology (3rd ed. Jaypee Brothers 2015).
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12
Giroud Mangin, Forensic Medicine: Clinical and Pathological Aspects. London, Greenwich Medical Media
j
(2003); V.K. Sharma, Poisons, Viscera Analysis, Report and its Interrelation, 6 International Journal of Medical
Toxicology and Legal Medicine 2 (2004).
13
supra note 2.
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7
poisoning may be excreted thorough lungs by evaporation. Organic solvent poison gets evaporated
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The poison is metabolized, detoxified, altered in the body and converts to non-toxic form giving the
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j negative analysis. There are many drugs, particularly anesthetic agents containing an ester bond,
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j which are unstable in biological tissues and susceptible to chemical or enzymatic hydrolysis. The
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j treatment given to patient may also alter the nature of poisonous substance.15
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It leads to chemical changes in certain poisons. Those poisons are then rendered identifiable by
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j chemical tests e.g. chloral hydrate, sodium nitrite, cocaine, aconite, atropine etc.16
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The detection of a highly potent poison with a low lethal dose is difficult. 17
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The proteinous poisons are rather impossible to extract after absorption in tissues. Similarly, the
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j extraction of water soluble compounds is very difficult. So, they are not detected from tissues by
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18
j chemical methods of analysis. j j j j
Leaking Jars, wrong preservative, insufficient quantity of samples, and wrong material of jars are
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14
Anil Agarwal, Textbook of Forensic Medicine and Toxicology (1st ed. Avichal Publishing Company 2017).
15
Frank T. Peters & Hans H. Maurer, Bioanalytical method validation and its implications for forensic and clinical
j
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3.1.10 Tampering of the Viscera j j j j j
It may be done during preservation and in preserved bottles with vested interests or wrong motive.
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j Addition of strong chemicals like soap or bleach powder alters the results in immunoassay.
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Cyanide, ethyl alcohol, ketones and sulphides may be formed from normal tissue components.
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j Although the cyanide so formed is always in trace amounts, the alcohol produced by advanced
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j decomposition may be upto 30 mg per cent. Some substances other than the poison may come j j j j j j j j j j j j j j j
21
j positive which was taken in the hospital for therapeutic reason like promethazine etc.
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3.2.1 Thallium j
It is difficult to isolate thallium in bodily fluids. The monovalent thalliumion, Tl+1, has properties
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j similar to that of the commonly present sodium and potassium ions, Na+1 and K+1 respectively,
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3.2.2 Polonium-210 j j
It is a rare and highly radioactive isotope. It is hard to detect because all the radiation remains in the
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j body. A lethal dose could be as little as a few milligrams, which could be administered as a powder
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j or dissolved in liquid.23
j j j
It contains oleandrine glycosides which cross-reacts during the positive results of digoxin
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j immunoassay.24
20
AS Curry & Sunshine, The liver: blood ratio in cases of barbiturate poisoning, 3 Toxicol Appl Pharmacol (1960).
j
21
Suzuki Watanabe, Drugs and poisons in humans: a handbook of practical analysis, Basel Journal (2005).
22
supra note 15.
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23
Harding Sample, Polonium-210: the hard-to-detect poison that killed Alexander Litvinenko, The Guardian (Aug.
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3.2.4 Snake Bites j j
It is not possible to detect the venom by chemical analysis as the venom is destroyed very fast.
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j Snake venom is a Protein and cannot be separated from body tissues. Immunoassay method may
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j detect these poisons but this facility is not easily available in all FSLs.25
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3.2.6 Heroin26 j j
The heroin (Diacetyl Morphine) is very difficult to detect as it is rapidly hydrolyzed to monoacetyl
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j morphine and morphine. Therefore, the samples should be tested for morphine.
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It was found that there had been multiple instances in the past wherein the medical evidence in the
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j viscera report has been insufficient to prove the guilt of the accused, however the Supreme Court
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j has held that a mere negative result of the viscera report does not rule out poison as the cause of
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The accused was convicted under Section 302.28 The autopsy surgeon had found no pathological
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j lesion in the pancreas, the kidney, the liver and any other internal organ. He gave the opinion after
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j the receipt of the negative Chemical Analyser’s report and hospital records that death could have
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j occurred due to diabetic coma. He later admitted in the court that he may have expressed incorrect
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j opinion. The court observed that if circumstantial evidence is so decisive that the Court can
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j unhesitatingly hold that death was a result of administration of poison (though not detected) and j j j j j j j j j j j j j j
j that the poison must have been administered by the accused person, then the conviction can be
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j rested on it. j j
“What assistance a man of science can give he gives, but it is too much to say that the guilt of
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j the accused must, in all cases, should be demonstrated by the isolation of the poison”.
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25
supra note 18.
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26
id.
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27
Anant Chintaman Lagu v. The State of Bombay, AIR 1960 SC 500.
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28
Indian Penal Code, 1860 § 302 (Act No. 45 of 1860).
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3.3.2 Palinoswamy v. State of Bombay29
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If the medical evidence is unable to detect/determine the poison even then the conviction can be
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The accused were convicted under Section 302. 31 The doctor who performed the post mortem
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j examination on the dead body gave evidence that the death of the deceased might have been a
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j natural death. He was declared hostile by prosecution in a case of alleged murder by poisoning. The
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j viscera report was negative. But still the court observed that “the pathologist’s role is secondary
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j and several poisons particularly synthetic hypnotics and vegetable alkaloid groups do not leave any
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j characteristic signs which can be noted on PM.” The court decided that mere non detection of the j j j j j j j j j j j j j j j j
The court observed that “The chemical examiner does not, as a rule, give an opinion as to the cause
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j of death but merely gives report of the chemical examination. The report itself is not crucial. The
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j report should normally be forwarded to the doctor who conducted the autopsy.
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In poison murder cases33, the accused are not acquitted solely on the ground that the prosecution has
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j failed to prove that the accused had the poison in his possession, and are to be acquitted by the
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In a case of 304B IPC, it was contended by the accused that the viscera report would have shown
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j whether the dowry death of the appellant’s wife occurred on account of consumption of poison.
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j Since the chemical examination report of the viscera was not received, it could not be said to be a
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j case of death by poisoning. The Supreme Court rejected the contention and decided that,
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29
Palinoswamy v. State of Bombay, AIR 1968 Bom 127.
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30
Mahabir v. State of Bihar, 1972 SCR (3) 639.
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31
Indian Penal Code, 1860 § 301 (Act No. 45 of 1860).
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32
Bhupender Singh v. State of Punjab, 1988 SCR (3) 409.
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Indian Penal Code, 1860 § 302 (Act No. 45 of 1860).
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Taiyab Khan and Others v. State of Bihar, 2005 13 SCC 455.
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“Since Section 304-B of the IPC refers to death which occurs otherwise than under normal
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j circumstances, the absence of a viscera report would not make any difference to the fate of the j j j j j j j j j j j j j j j j
j case”. j
The autopsy surgeon stated that the unnatural death was due to the effect of poisoning but he would
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j be able to conclusively state the cause of death by poisoning only, if he could detect poison in the
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j viscera report. This Court noted that it was not in dispute that the death was an unnatural death and
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j held that the deposition of the witness indicated that the death was due to poisoning. It is only the
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j nature of the poison that could not be identified. In view of this, the conviction of the appellant
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The autopsy surgeon had given the cause of death as suspected poisoning. The accused contended
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j that in absence of viscera report the death could not be attributed to poisoning. The Supreme Court
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j observed that “In a case of an unnatural death inviting Section 304-B of the IPC or Section 306 of
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j the IPC as long as there is evidence of poisoning, identification of the poison may not be absolutely
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j necessary. Even when a viscera report is sought for, its absence is not necessarily fatal to the case”
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The Calcutta High Court recently appointed an amicus curiae or ‘friend of the court’ to assist it in
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j getting answers related to Viscera preservation and examination. “What comprises viscera, how
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j long can it be preserved and what is the law governing its examination at forensic science
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j laboratories?” The amicus curiae was told by Experts that a viscera can be preserved only if j j j j j j j j j j j j j j j
j properly refrigerated. If preserved in common salt water, as it is normally done, the sample will
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j decompose in six months. The amicus curiae has also cited a Supreme Court order which directsj j j j j j j j j j j j j j j
35
j Ananda Mohan Sen and Another v. State of West Bengal, 2007 10 SCC 774.
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j Bhupendra v. State of Madhya Pradesh, Cri Appeal No. 1774 of 2008. j j j j j j j j j j j
12
j that a viscera should be sent to a forensic science laboratory immediately if poisoning is
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j suspected.37
This study disproves the hypothesis that poison as the cause of death is ruled out merely because of
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j a negative viscera report. It is the personal opinion of the author that currently, the investing police
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j officers are still under the impression that a negative viscera report means that poison could not
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j have been the cause of death. There are several poisons which cannot be detected by a common test
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j and it is not practical to conduct separate tests for each and every poison in the world. The several
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j judgments discussed in this paper also prove that the judges agree that just because a poison has not
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j been identified in the report, does not mean that there was a natural cause of death. The totality of
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j the circumstances are considered, which is why this erroneous assumption should not exist in the
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j minds of officers conducting the inquiry. Further suggestions to amend this current scenario have
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CHAPTER 4: SUMMARY J J
It is thus very clear that the hypothesis has been disproven and a negative viscera report does not
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j rule out the possibility of a cause of death by poison. In order for there to be a clearer understanding
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j regarding this conclusion amongst the investigating officers, some recommendations have been put
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• It is practically not possible to undertake test for each and every poison, so the police
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j investigators should be meticulous in their approach. They should also give a brief description j j j j j j j j j j j j j
j of the case highlighting the aspects which may point to the nature of poison consumed. It may
j j j j j j j j j j j j j j j j
37
J. Gupta, HC appoints ‘amicus curiae’ to help it determine what ‘viscera’ is and how long it can be preserved, The
Times of India. (Dec 5th 2013, 5:05 pm), http://timesofindia.indiatimes.com/topic/ Hc-Appoints-‘Amicus-Curiae’-
To-Help-It-Determine-What-‘Viscera’.
13
j include crime scene report, statement of the relatives/friends, recent purchases of
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• There should be a designated time frame for the police to deposit the viscera in FSL after
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j Postmortem.
• There should be a separate storage facility for the preservation of toxicological specimens at a
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• Strict operating guidelines should be enforced to preserve vomitus and gastric lavage samples,
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j in the casualty of the hospital/ any medical center where the patient first presents.
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• In an admitted case, the blood and urine samples should be collected during the first hours of
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j hospital stay. j
• A detailed history, including treatment summary should be provided to the FSL’s so as to guide
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j them to run tests for those poisons which are not detected in routine examination.
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4.2 CONCLUSIONJ J
The investigating police officers and the supervising senior police officers must have a very clear
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j concept that mere a negative viscera report does not rule out death due to poisoning, as the viscera
j j j j j j j j j j j j j j j j j
j tests has a lot of limitations and restricted to very few common poisons. It should not lead to the end
j j j j j j j j j j j j j j j j j j j
j of the investigation into a death due to poisoning. Moreover, FSLs should clearly specify in their
j j j j j j j j j j j j j j j j
j reports the exact name of poisons for which the tests have been conducted.
j j j j j j j j j j j j j
The laboratory should mention the fallacies and limitations of the tests in their reports. This will
j j j j j j j j j j j j j j j
j prevent the misinformation to the investigators/ relatives who are generally in the impression that a
j j j j j j j j j j j j j j
j negative viscera report rules out death by poisoning. The FSL must have accreditation, its
j j j j j j j j j j j j j
j equipments and methods of analysis should be incorporated in report with the credential ofj j j j j j j j j j j j j
j scientist. As per judgments of the apex court and the facts described above, the autopsy surgeon
j j j j j j j j j j j j j j j
j should not be deterred by mere non-detection of poison in the chemical analysis of viscera. The
j j j j j j j j j j j j j j j j
j doctor who has conducted the postmortem examination depending upon PM findings, the medical
j j j j j j j j j j j j
j records, circumstantial evidences and after ruling out any other cause of death can very well give
j j j j j j j j j j j j j j j
14
REFERENCES
BOOKS
• AK Jaiswal & Millo, Handbook of Forensic Analytical Toxicology, (3rd ed. Jaypee
Brothers 2015).
• ANIL AGARWAL, Textbook of Forensic Medicine and Toxicology, (1st ed. Avichal
Publishing Company 2017).
• CK Parikh, Textbook of Medical Forensic Medicine and Toxicology (6th ed. CBS
Publishers 1999).
• K Matiharan & AK Patnaik, Modi’s Medical jurisprudence and Toxicology (5th ed. Lexis
Nexis 2010).
• K Vij., Textbook of Forensic Medicine and Toxicology: Principles and Practice (5th ed.
Elsevier Publishers 2011).
• KSN Reddy, The essentials of Forensic Medicine and Toxicology (29th ed. Sugana Devi
Publishers 2018).
JOURNAL ARTICLES
• AS Curry & Sunshine, The liver: blood ratio in cases of barbiturate poisoning, 3 Toxicol
Appl Pharmacol (1960).
• Danielle M. Butzbach, The influence of putrefaction and sample storage on post-mortem
toxicology results, 45 Forensic Science Medical Pathology Journal (2010).
• Dr. Abhishekh Yadav, Death due to Poisoning: Intricacies Thereof, 62 The Indian Police
Journal 1 (2016).
• Frank T. Peters & Hans H. Maurer, Bioanalytical method validation and its implications
for forensic and clinical toxicology – A review, 68 Society for Forensic and Toxicologic
Chemistry (2002).
• Giroud Mangin, Forensic Medicine: Clinical and Pathological Aspects. London,
Greenwich Medical Media (2003).
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• HM Stevens, The stability of some drugs and poisons in putrefying human liver tissues,
24 Journal of Forensic Science (1984).
• K Cheung K & P Duffy, Detection of poisoning by plant-origin cardiac glycoside with
the Abbott TDx analyzer, 35 Clinical Chemistry Journal of Forensic Science 2 (1989).
• Lokan James & Dymock, Apparent post-mortem production of high levels of cyanide in
blood, 9 Journal of Forensic Science (1987).
• OH Drummer, Post-mortem toxicology, 165 Forensic Science International Journal
(2007).
• Suzuki Watanabe, Drugs and poisons in humans: a handbook of practical analysis, Basel
Journal (2005).
• V.K. Sharma, Poisons, Viscera Analysis, Report and its Interrelation, 6 International
Journal of Medical Toxicology and Legal Medicine 2 (2004).
• VA Boumba & KS Ziavrou, Biochemical pathways generating post-mortem volatile
compounds co-detected during forensic ethanol analyses, 52 Forensic Science
International Journal (2008).
NEWSPAPER ARTICLES
• Harding Sample, Polonium-210: The hard-to-detect poison that killed Alexander
Litvinenko, The Guardian, (Aug 8th 2014, 1:06 pm).
• J. Gupta, HC appoints ‘amicus curiae’ to help it determine what ‘viscera’ is and how long
it can be preserved, The Times of India, (December 5th 2013, 5:05 pm).
CASES
• Ananda Mohan Sen and Another v. State of West Bengal, 2007 10 SCC 774.
• Anant Chintaman Lagu v. The State of Bombay AIR 1960 SC 500.
• Bhupender Singh v. State of Punjab, 1988 SCR (3) 409.
• Bhupendra v. State of Madhya Pradesh, Cri Appeal No. 1774 of 2008.
• Mahabir v. State of Bihar, 1972 SCR (3) 639.
• Palinoswamy v. State of Bombay, AIR 1968 Bom 127.
• Taiyab Khan and Others v. State of Bihar, 2005 13 SCC 455.
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STATUTES
• Indian Penal Code, 1860 (Act No. 45 of 1860).
• The Code of Criminal Procedure, 1973 (Act No. 2 of 1974).
LEGAL DATABASES
• Manupatra.
• SCC Online.
• Jstor.
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