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    100-36E Lot# 0613410

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    fluffy4202011
    This document provides a certificate of analysis for recombinant human TGF-β3. It describes the product as a 25kDa protein composed of two identical polypeptide chains that regulates cell proliferation, growth, differentiation and motility. Storage and handling instructions are provided, noting the product should be reconstituted in citric acid and stored at -20 to -80°C for extended periods. Specifications confirm the product is sterile filtered and >98% pure as verified through various analyses.

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    100-36E Lot# 0613410

    Uploaded by

    fluffy4202011
    23 views1 page
    This document provides a certificate of analysis for recombinant human TGF-β3. It describes the product as a 25kDa protein composed of two identical polypeptide chains that regulates cell proliferation, growth, differentiation and motility. Storage and handling instructions are provided, noting the product should be reconstituted in citric acid and stored at -20 to -80°C for extended periods. Specifications confirm the product is sterile filtered and >98% pure as verified through various analyses.

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    This document provides a certificate of analysis for recombinant human TGF-β3. It describes the product as a 25kDa protein composed of two identical polypeptide chains that regulates cell proliferation, growth, differentiation and motility. Storage and handling instructions are provided, noting the product should be reconstituted in citric acid and stored at -20 to -80°C for extended periods. Specifications confirm the product is sterile filtered and >98% pure as verified through various analyses.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    23 views1 page

    100-36E Lot# 0613410

    Uploaded by

    fluffy4202011
    This document provides a certificate of analysis for recombinant human TGF-β3. It describes the product as a 25kDa protein composed of two identical polypeptide chains that regulates cell proliferation, growth, differentiation and motility. Storage and handling instructions are provided, noting the product should be reconstituted in citric acid and stored at -20 to -80°C for extended periods. Specifications confirm the product is sterile filtered and >98% pure as verified through various analyses.

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    © All Rights Reserved
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    Princeton Business Park, 5 Crescent Ave., P.O.

    Box 275
    Rocky Hill, NJ 08553 USA
    Tele: 609.497.0253 Fax: 609.497.0321
    www.peprotech.com Email: info@peprotech.com
    Certificate of Analysis (CoA)
    Recombinant Human TGF-β3
    Catalog# 100-36E Expiration Date: December 2020
    Lot# 0613410 Source: E.coli
    Synonyms: Transforming Growth Factor-β3
    Description: The three mammalian isoforms of TGF-β, TGF-β1, β2, and β3, signal through the same receptor and elicit similar
    biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well
    as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes, including
    embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the
    cell surface and in the extracellular matrix. The release of the biologically active TGF-β isoform from a latent complex involves
    proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. The
    physiological role of TGF-β3 is still unknown, but its expression pattern suggests a role in the regulation of certain development
    processes.--Recombinant Human TGF-β3 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked
    by a single disulfide bond.
    Sequence-(monomer):-ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP
    QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

    Storage & Handling:


    Reconstitution: Centrifuge vial prior to opening. Reconstitute in 5-10mM Citric Acid to 0.1-1.0 mg/ml. Do not
    vortex. Store at 2°C to 8°C for 1 week, or prepare for extended storage.
    Extended Storage: Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA).
    Store working aliquots at -20˚C to -80˚C. Avoid repeated freeze-thaw cycles.
    Storage/Stability: Product Form Temperature Storage Time
    Lyophilized -20˚ to -80˚C December 2020
    Lyophilized 4°C 6 months
    Lyophilized Room Temperature 1 month
    Reconstituted 2°C to 8°C 1 week
    Extended Storage -20˚ to -80˚C 12 months
    Specifications:
    Formulation: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.1% TFA.
    Authenticity: Verified by N-terminal and Mass Spectrometry analyses (when applicable).
    Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
    Endotoxin: Endotoxin level is < 0.1 ng/μg of protein (< 1 EU/μg).
    Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel.
    Biological Activity: Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The
    expected-ED50-is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107-unit/mg.
     

    Usage: Not for human use.


    Country of Origin: USA

    Quality Assurance Representative


     

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