The Nutritional Requirements of Infants. Towards EU Alignment of Reference Values: The EURRECA Network

DOI: 10.1111/j.1740-8709.2010.00262.
Original Article
The nutritional requirements of infants. Towards EU
alignment of reference values: the EURRECA network mcn_262 55..83
Maria Hermoso*, Garden Tabacchi†, Iris Iglesia-Altaba‡, Silvia Bel-Serrat‡,

Luis A. Moreno-Aznar‡, Yurena García-Santos§, Ma del Rosario García-Luzardo§,
Beatriz Santana-Salguero§, Luis Peña-Quintana§¶, Lluis Serra-Majem¶,
Victoria Hall Moran**, Fiona Dykes**, Tamás Decsi††, Vassiliki Benetou‡‡, Maria Plada‡‡,
Antonia Trichopoulou‡‡, Monique M. Raats§§, Esmée L. Doets¶¶, Cristiana Berti***,
Irene Cetin*** and Berthold Koletzko*
*Division of Metabolic Diseases and Nutritional Medicine. Dr von Hauner Children’s Hospital, Ludwig-Maximilians-University of Munich, Munich,
Germany, †Institute of Physiology and Human Nutrition, University of Palermo, Palermo, Italy, ‡‘Growth, Exercise, Nutrition and Development’ (GENUD)
Research Group, Escuela Universitaria de Ciencias de la Salud, Universidad de Zaragoza, Zaragoza, Spain, §Unidad de Gastroenterología, Hepatología y
Nutrición Pediátrica. Hospital Universitario Materno-Infantil de Canarias. Las Palmas de Gran Canaria, Spain, ¶Departamento de Ciencias Clínicas.
Universidad de Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, Spain, **Maternal and Infant Nutrition and Nurture Unit (MAINN),
University of Central Lancashire, Preston, UK, ††University of Pécs, Pécs, Hungary, ‡‡Department of Hygiene, Epidemiology and Medical Statistics, School of
Medicine, National and Kapodistrian University of Athens, Athens, Greece, §§Department of Food, Consumer Behaviour and Health, University of Surrey,
Guilford, UK, ¶¶Division of Human Nutrition, Wageningen University and Research Centre, Wageningen, the Netherlands, and ***Unit of Obstetrics and
Gynecology, Department of Clinical Sciences Hospital ‘L. Sacco’, and Center for Fetal Research Giorgio Pardi, University of Milan, Milano, Italy
Abstract
This paper presents a review of the current knowledge regarding the macro- and micronutrient requirements of
infants and discusses issues related to these requirements during the first year of life. The paper also reviews the
current reference values used in European countries and the methodological approaches used to derive them by
a sample of seven European and international authoritative committees from which background scientific
reports are available. Throughout the paper, the main issues contributing to disparities in micronutrient refer-
ence values for infants are highlighted. The identification of these issues in relation to the specific physiological
aspects of infants is important for informing future initiatives aimed at providing standardized approaches to
overcome variability of micronutrient reference values across Europe for this age group.
Keywords: nutrient requirements, nutrient recommendations, recommended intakes, nutrient intake values,
infants, EURRECA.
Correspondence: Maria Hermoso, Division of Metabolic Diseases and Nutritional Medicine, Dr von Hauner Children’s Hospital.
Ludwig-Maximilians-University of Munich, Lindwurmstr. 4, 80337 Munich, Germany. E-mail: maria.hermoso@med.uni-muenchen.de
Background reference values for intake (Doets et al. 2008). Infants

are considered a vulnerable group because they have
Different countries and committees have set micro- a relatively high requirement of nutrients per unit
nutrient reference values for infancy, i.e. up to 1 year body weight during a sensitive period of rapid growth
of age. These values vary considerably across Europe. and development. The aim of the present paper is to
The variability is caused by various factors, e.g. review specific aspects of healthy term infants in rela-
disparities exist between underlying concepts, the ter- tion to micronutrient requirements in early and late
minologies used and the methodologies and assump- infancy, and to critically evaluate the approaches used
tions that have been made in the definition to determine those requirements and derived refer-
of micronutrient requirements and derivation of ence values for nutrient intake.
© 2010 Blackwell Publishing Ltd Maternal and Child Nutrition (2010), 6 (Suppl. 2), pp. 55–83 55
56 M. Hermoso et al.
1. Nutritional requirements of and experienced less morbidity from gastrointestinal

infants, methodological approaches infection. Recent expert committee reports in Europe
used for establishing requirements and in the USA concluded that complementary
and derived reference values feeding, in addition to continued breastfeeding,
should be introduced not before 17 weeks and not
Infancy is characterized by rapid growth and devel- later than 26 weeks of age (Agostoni et al. 2008; Greer
opment. Both are determined by genetic and environ- et al. 2008). The comparative benefits of exclusive
mental factors. An important environmental factor is breastfeeding for six months compared with exclusive
nutrition, because an inadequate diet can compro- breastfeeding for between 4 and 6 months, particu-
mise growth and the full utilization of an individual’s larly in infants living in industrialized countries,
genetic potential (Reyes-Posso 2008). Nutrition is however, has been challenged (Fewtrell et al. 2007). In
important throughout childhood, but especially Kramer & Kakuma’s paper (2002), only two of 20 the
during the first 2 years of life, as the growth rate studies included in the review were randomized inter-
during this period is high and less dependent on vention trials of different exclusive breastfeeding
growth hormones than in later periods of childhood. recommendations, and both were conducted in non-
The rapid rates of growth and development of infants industrialized countries (Cohen et al. 1994; Dewey
impose unique nutritional needs upon their already et al. 1999). Due, in no small part, to clear ethical and
high maintenance needs (Heird 1996). The quantity feasibility issues of conducting such trials, the overrid-
and quality of nutrient supply during early life modu- ing majority of studies in the review were observa-
lates the differentiation of tissues and organs and has tional and therefore vulnerable to considerable
short- and long-term consequences for health residual confounding and bias. Indeed, other system-
(Koletzko 2008). atic reviews have suggested that breast milk may not
The preferred form of nutrition for healthy infants meet the full requirements for energy and certain
is breastfeeding (Agostoni et al. 2009). Current global micronutrients of 6-month-old infants (Butte 2002;
recommendations, derived from a technical expert Reilly et al. 2005). Clearly, there is an urgent need for
committee report commissioned by the World Health more work in this area.
Organization (WHO) (Kramer & Kakuma 2002), are Despite the multiple benefits of breastfeeding, a
that infants should be exclusively breastfed for the significant proportion of mothers of healthy term
first 6 months of life with the introduction of comple- infants in many industrialized countries chose to
mentary foods thereafter and continued breastfeed- formula feed their infants. There is a lack of guidance
ing for the first 2 years of the infant’s life (WHO regarding the appropriate timing of the introduction
2003b). This report concluded that infants who were of complementary foods to formula fed infants
exclusively breastfed for 6 months did not differ in because there is virtually no data available to form
growth from those exclusively breastfed for 4 months evidence-based recommendations. It has been argued
Key messages
• Infancy is characterized by growth and development, which impose unique nutritional needs upon the already
high maintenance needs of infants.
• Primary data from quality studies linked to functionally relevant outcomes are needed in infants in order to
improve the current knowledge on nutritional requirements in this period of life.
• Great disparities exist in established nutrient intake values for infants in Europe. This is mainly due to the
different methodological approaches used to define the nutritional requirements of infants. A lack of trans-
parency in the documentation of the decision making by the committees in charge hampers understanding
these differences.
• Solid, up-to-date, transparent scientific basis will help committees tasked with setting reference values for
nutrients, defining requirements of infant formula, and defining feeding practice.
© 2010 Blackwell Publishing Ltd Maternal and Child Nutrition (2010), 6 (Suppl. 2), pp. 55–83
Nutritional requirements of infants 57
that, as formula-fed infants receive higher amounts of to a number of aspects of food and nutrition policy
energy, protein, iron and zinc than breastfed infants, such as regulatory issues and trade, labelling, planning
they may not require solid foods until a later age programmes for alleviating public health nutrition
(Fewtrell et al. 2007). Despite this, across Europe, problems, food fortification and dietary guidance
complementary feeding is introduced earlier in (Aggett et al. 1997). NIVs are based on physiological
formula fed than in breastfed infants (Schiess et al. requirements, which are defined by the European
2010). It has been hypothesized that an early intro- Society of Paediatric Gastroenterology, Hepatology
duction of complementary foods to formula fed and Nutrition (ESPGHAN) as the amounts and
infants might increase the risk of later overweight and chemical forms of nutrients needed systematically to
obesity in children and adults. There is evidence to maintain normal health and development, without
suggest that formula feeding is associated with a more disturbance of the metabolism of any other nutrient
rapid weight gain in early infancy (Dewey 2001; and without extreme homeostatic processes and
Baker et al. 2004) and with an increased risk for excessive depletion or surplus of bodily reports
obesity in childhood and adolescence (Gillman et al. (Aggett et al. 1997).The nutrient requirements of indi-
2001; von Kries et al. 2001; Arenz et al. 2004; Koletzko viduals vary markedly, and NIVs do not refer to indi-
et al. 2009b). Higher intakes of protein and energy viduals, but to populations that are defined by age,
with infant formula compared with breast milk may gender and in some cases additional characteristics
favour rapid weight gain, adipogenic development of relevant for nutrient needs. Existing NIVs for infants
adipocytes and the accumulation of fat (Koletzko are shown and compared in section 2.
et al. 2009a). This could lead to increased likelihood of The UNU Working Group has defined the following
later overweight, but it is as yet unclear whether an NIVs: average nutrient requirement (ANR), indi-
earlier introduction of complementary foods plays a vidual nutrient level (INL) and upper nutrient level
relevant role. (UNL). ANR is the estimated average or median
requirement of a specific nutrient in a population,
derived from a statistical distribution of requirement
1.1 Concepts and use of nutrient intake values
criterion and for a particular age and sex specific group
The European Micronutrient Recommendations based on a specific biological endpoint or biochemical
Aligned (EURRECA) Network of Excellence works measure (King & Garza 2007). It is assumed that
similarly towards a general framework including har- individual requirements follow a statistical distribu-
monized approaches, methods and key terms to be tion (symmetrical bell-shaped curve). INL is the indi-
used for the development of micronutrient reference vidual nutrient level and INL97 is the nutrient intake
values (Doets et al. 2008).The United Nations Univer- considered adequate to meet the known nutrient
sity (UNU), in collaboration with the Food and needs of practically all healthy individuals in a particu-
Agricultural Organisation (FAO), World Health lar age and sex-specific group (King & Garza 2007).
Organisation (WHO) and the United Nations Chil- Equivalent terms are population reference intakes
dren’s Emergency Fund (UNICEF), has previously and recommended dietary allowance.This value, set at
proposed an international harmonization of nutrient a level of intake that meets the needs of the majority of
based dietary standards (King & Garza 2007). The the population (mean + 2SD), is generally used as the
term Nutrient Intake Values (NIVs), conceived by target for provision of essential nutrients to popula-
UNU, FAO, WHO and UNICEF, encompass all tions and as the reference point for nutrient labelling
nutrient-based dietary standards derived from of foods.The exception is energy, for which the ANR is
primary data and provide estimates on appropriate used because the use of INL97 or equivalents would
dietary substrate supply for populations of healthy lead to overfeeding. UNL is the highest level of daily
people (King & Garza 2007). NIVs are used for assess- nutrient intake that is likely to pose no risk of adverse
ing the adequacy of nutrient intakes and for planning health effects for almost all individuals of a particular
diets of groups and individuals. They are also applied life-stage group (King & Garza 2007).
1.2 Methods used to estimate the nutritional the total body content of that nutrient from one age
requirements of infants and derived NIVs to another) can be calculated, taking into account the
metabolic cost of accretion. However, very limited
Indicators of function provide information to target direct analytical information on body composition is
requirements for health protection and not just to available, which cannot be reliably extrapolated
prevent clinical deficiencies. However, there are a between age groups. Maintenance needs are derived
limited number of functionally relevant outcome from estimates of losses related to cellular turnover
measures for infants that reflect the response to and unavoidable metabolic inefficiency. Data on
dietary intake (IOM 2000b). The first months of life inevitable losses in newborns, toddlers and infants are
pose particular challenges with regards to clinical scant. Given that in adults such information is best
research, and few quality studies have been per- gained under circumstances of negligible intakes of
formed in this age group. The paucity of available the nutrient of interest when homeostatic conserva-
data for estimating nutrient requirements of infants tion is maximal, it is unlikely that such data could be
hampers the establishment of adequate NIVs (Pombo acquired ethically in infants (Aggett et al. 1997).
et al. 2001). Usual intakes of presumably healthy Balance studies at known intakes provide informa-
populations, factorial approaches and balance tech- tion about net whole retention and net intestinal
niques are the methods used most often to estimate absorption or secretion and whole-body retention of
nutrient needs of infants. None are fully satisfactory nutrients. These studies are difficult to perform in
because they seldom adequately address the issue of infants, although some studies have been performed
nutrient intake supporting long-term health and in term and pre-term infants. One limitation is that
optimal functional capacities rather than just avoiding they tend to overestimate net retention and thus
acute deficiency states (Butte 2002). underestimate requirements due to technical difficul-
NIVs for infants during the first 6 months of life are ties in sampling. To extrapolate nutrient requirements
usually derived from estimated intakes of fully breast- from balance studies, subjects should be in equilib-
fed infants. For older infants (7–12 months), estimates rium at the intake of the nutrient in question, which is
of intakes from both human milk and complementary difficult to determine in periods of fast growth. The
foods are used as a reference. However, the actual intake has to be manipulated so that it balances losses.
intakes of breastfed infants are difficult to determine The length of the study period also depends on the
due to the variability of milk volume and composition size of the body stores of the nutrient and the rate at
between women, as well as the changing composition which the stores are mobilized (Prentice et al. 2004).
of the milk during the course of lactation, during the The interpretation of balance studies often relies
day and even during a feeding. Moreover, the bio- heavily on estimates derived by factorial approaches,
availability of substrates and their metabolism differ that is, the appropriateness of retained quantities of
between infants fed human milk, those fed infant target nutrients is determined by comparison with
formula and infants given complementary feeds. expected retention based on estimates derived by fac-
Therefore, the composition of human milk and the torial methods. Thus, estimates of growth velocity and
nutrient intake of breastfed infants do not always tissue composition are key to interpreting balance
provide useful guidance for infants who are not exclu- results (Butte 2002). The use of isotopic labels of
sively breastfed (Aggett et al. 1997). endogenous or exogenous (dietary) pools of nutrients
Factorial approaches are generally based on esti- can enable better characterization of the flux and pool
mates of maintenance needs, nutrient accretion that sizes underlying homeostasis. Thus, the balance-study
accompanies growth, measures of digestibility and/or approach, with these refinements, is likely to remain a
absorption (bioavailability), and utilization efficiency. key method in investigating requirements until new
The requirements for growth are derived from esti- methods become established (Aggett et al. 1997).
mates of body composition at different ages, from Extrapolation and interpolation methods are often
which the nutrient accretion over time (increase in employed to derive NIVs for infants, especially from 7
months of age. Values for nutrient needs are extrapo- Elango 2008). The average protein content of human
lated from one life stage to another using weighting milk is 11.7 g/L-1 (Pencharz & Elango 2008). Exclusive
for body size, energy requirement and other meta- breastfeeding meets the protein and amino acid
bolic differences. These approaches have several limi- requirements during the first 4–6 months of life.
tations, as outlined elsewhere (Atkinson & Koletzko During the second 6 months of life, solid foods contrib-
2007). The rationale for the method chosen for ute a significant amount of protein to the infant diet.
extrapolating NIVs should be completely transparent The biological value of a protein refers to its ability,
and described in detail for each nutrient (Atkinson & when it is the sole dietary source of protein, to support
Koletzko 2007). protein synthesis and therefore body maintenance and
growth. On this scale, breast milk proteins and egg
1.3 Nutrient requirements of infants for energy, have the highest value (1.0). All animal proteins (with
macro- and micronutrients the exception of gelatin) are complete, that is they
contain all the essential amino acids and are of high
1.3.1 Energy and macronutrients requirements
biological value. Most vegetable proteins, except soya,
Energy are incomplete because they offer an unbalanced
assortment of amino acids that cannot alone satisfy the
During infancy, energy requirements are defined as
body’s needs (Michaelsen et al. 2000).
the amount of energy needed to balance total energy
expenditure at a desirable level of physical activity,
and to support optimal growth and development con- Lipids
sistent with long-term health (FAO/WHO/UNU
Fats are the main source of energy for infants, and n-6
2004). Energy requirements during growth and devel-
and n-3 long-chain polyunsaturated fatty acids
opment can be partitioned into components of basal
(LCPUFAs) are essential for normal growth and
metabolism, thermogenesis, physical activity and
development and maturation of numerous organ
energy cost of growth (Butte 2005). The energy
systems, most importantly the brain and eye. More-
needed for growth is estimated to be around 35% of
over, lipid-soluble vitamins (A, D, E, K) require
the total energy requirement in the first month of life,
dietary lipids for absorption (Mena & Uauy 2008).
and this requirement declines continuously to about
Exclusively breastfed infants receive a dietary fat
3% at 12 months, remaining low until the onset of the
supply usually in the range of 40–55% of total dietary
pubertal growth spurt (Butte 2005).
energy intake (Michaelsen et al. 1990). The introduc-
tion of complementary feeding that is rich in carbohy-
Protein
drates may reduce fat intake to 30–40% of energy
The protein requirement of infants can be defined as intake. For infant formula a fat content in the range of
the minimum intake that will allow nitrogen equilib- 40–60% of total energy content has been recom-
rium at an appropriate body composition during mended (Scientific Committee on Food 2003). This
energy balance at moderate physical activity, plus the corresponds to a fat content of 4 to 6 g/100 kcal as
needs associated with the deposition of tissues consis- established by the European Commission Directive
tent with good health (WHO/FAO/UNU 2007). The on Infant Formulae and Follow on Formulae (Euro-
nine essential amino acids (leucine, isoleucine, valine, pean Commission 2006). Human milk provides
tryptophan, phenylalanine, methionine, threonine and linoleic acid (LA), alpha-linoleic acid (ALA), docosa-
histidine) need to be obtained from the diet. The con- hexaenoic acid (DHA), arachidonic acid (AA), and
ditionally essential amino acids (arginine, cysteine, other LCPUFAs to breastfed infants. The level of AA
glutamine, glycine, proline and tyrosine) are those that is relatively constant on a worldwide basis whereas the
the infant in unable to produce in sufficient amounts level of DHA is more variable and depends on mater-
and hence all or part of the daily needs for those amino nal diet and lifestyle (Koletzko et al. 2008). Recent
acids must be provided through the diet (Pencharz & consensus recommendations support that pregnant
and lactating women should achieve a DHA intake enteral formulas and breast milk substitutes consumed
of at least 200 mg/d (Koletzko et al. 2007). The by infants. Additionally, some resistant starches and
ESPGHAN recommends the following content of non digestible carbohydrates are formed during pro-
essential fatty acids in infant formula: 0.3–1.2 g/ cessing (Aggett et al. 2003). The overall benefits to
100 kcal (2.7–10.8% total energy) of LA and 0.5–2.4 g/ health of supplementing infant formula or infant solid
100 kcal (0.54–2.59% total energy) of ALA, with a foods with fibre, inulin (probiotics) or prebiotic oli-
minimum LA/ALA ratio of 5:1 and a maximum of gosaccharides remain unclear (Agostoni et al. 2004;
15:1 (Koletzko et al. 2005). The authors of a recent Kien 2008).
review conclude that the available evidence supports
the addition of DHA to infant formula: the addition of 1.3.2 Micronutrient requirements
at least 0.2% of fatty acids as DHA appears necessary
Iron
for achieving a benefit on functional endpoints, but
DHA levels should not exceed 0.5% of fatty acids During the first year of life, the body iron content
because systematic evaluation of higher levels of increases markedly. In healthy term infants, iron stores
intake have not been published (Koletzko et al. 2008). at birth comprise most of the iron requirements during
Based on current knowledge, infant formula contents the first 4–6 months. From the 4th month, the require-
of AA should be at least those of added DHA, and ment for dietary iron increases to an estimated
eicosapentaenoic acid (EPA) should not exceed 0.78 mg/day due to the stepwise depletion of endog-
levels of DHA (Koletzko et al. 2008). enous stores and rapid growth with an expansion of
Examples of reference values used in European blood volume and increased tissue and storage iron
countries for average daily energy, protein, fat (AAP 1999; Fisher et al. 2000; IOM 2000b). Iron defi-
and essential fatty acids intakes in populations of ciency in humans is most prevalent in the late infancy
healthy infants set by different European and inter- period, which is characterized by peak hippocampal
national committees is presented for comparison in and cortical regional development, as well as myelo-
Table 1. genesis, dendritogenesis and synaptogenesis in the
brain where iron availability may play a role (Lozoff &
Georgieff 2006). Although the iron content of breast
Digestible and indigestible carbohydrates
milk is low, its bioavailability is high and needs of
Lactose is the main digestible carbohydrate in human dietary iron are limited due to the large iron stores of
milk, providing about 40% of the energy content newborn infants born at term, therefore iron defi-
(Koletzko et al. 2005). In addition to lactose, mature ciency at 6 months is uncommon in exclusively breast-
breast milk contains a large variety of oligosaccharides fed, term infants in industrialized countries (Yang et al.
in concentrations of approximately 5–10 g/L-1 (Kunz 2009). It has been shown that the same is true for
et al. 2000). Human milk oligosaccharides may prevent healthy full-term born infants fed exclusively an infant
bacterial adhesion by interfering with the docking of formula with only 1.6 mg iron/L-1 (Hernell & Lönner-
bacteria on the intestinal cell surface and with the dal 2002); despite this most modern infant formulae
expression of certain enzymes in the intestine required are fortified with iron. A recent study has shown that
for bacterial adhesion (Bode 2006). The total digest- while iron supplementation of breastfed infants
ible carbohydrate content of infant formula and caused some preservation of the iron endowment, the
follow-on formula is set between 9 and 14 g/kcal,which effect was modest and did not extend beyond the
is based on the calculated glucose consumption of the period of supplementation (Ziegler et al. 2009). The
central nervous system (Scientific Committee on Food study was underpowered to investigate any adverse
2003; European Commission 2006). Non-digestible affects of supplementation. However, other studies
carbohydrates such as fructooligosaccharides, galac- have reported adverse effects of iron supplementation
tooligosaccharides, inulin, soy polysaccharide, resis- in iron replete infants, for example on length growth
tant starch, and gums are added to dietary products, (Domellof 2007). Due to the low prevalence of iron
Table 1. Comparison of reference values for average daily energy, protein, fat and essential fatty acids intakes by different countries and committees
in populations of healthy infants
Age Energy Protein Fat Essential fatty acids
Male/female Male/female Male/female n-6 n-3
DACH[1] Month kcal/d g/d % of energy % of energy

0–<4 0–1 500 / 450 12 45–50 4.0 0.5
1–2 700 / 700 10
2–4 10
4–<12 4–6 95 10 35–45 3.5 0.5
6–12 10
NNR[2] Month MJ/d % of energy % of energy % of energy
<6 0–1 1.3 / 1.4 – – – –
3 2.1 / 2.2
6–11 6 2.6 / 2.7 7–15 30–45 4 1
12 3.4 / 3.7
UK[3] Month kcal/d g/d
0–<3 545 / 515 12.5 – –
4–<6 690 / 645 12.7 – –
7–<9 825 / 765 13.7 – –
10–<12 920 / 865 14.9 – –
USA/Canada[4] Month kcal/d g/d g/d g/d
0-<6 1 472 / 438 9.1 31 4.4 0.5
2 567 / 500
3 572 / 521
4 548 / 508
5 596 / 553
6 645 / 593
7-<12 7 668 / 608 11 30 4.6 0.5
8 710 / 643
9 746 / 678
10 793 / 717
11 817 / 742
WHO/FAO/UNU[5a, 5b] Month kcal/d g/d % of energy
3–6 700 13
6–9 810 14 30–40
9–12 950 14
The Netherlands[6] Month MJ/[kg.d] g/d % of energy g/day
0–2 0.39 9/8 45–50 0.64 –
3–5 0.35 10/9 45–50 0.64 –
6–11 0.35 10/10 40 0.15–0.2
[1]
German Nutrition Society (DGE),Austrian Nutrition Society (OGE), Swiss Society for Nutrition Research (SGE), Swiss Nutrition Association
(SVE) (2000) Referenzwerte für die Nahrstoffzufuhr/Reference Values for Nutrient Intake, 1st edition, 3 vollständig durchgesehener und
korrigierter Nachdruck 2008. Frankfurt am Main: Umschau/Braus.
[2]
Nordic Council of Ministers (2004) Nordic Nutrition Recommendations 2004, 4th edition: Integrating nutrition and physical activity.
Copenhagen.
[3]
Department of Health (1991) Dietary Reference Values for Food Energy and Nutrients for the United Kingdom. Report on Health and Social
Subjects 41. Report of the Panel on Dietary Reference Values of the Committee on Medical Aspects of Food Policy. London: HMSO.
[4]
Food and Nutrition Board, Institute of Medicine (2005) Dietary Reference Intakes for Energy, Carbohydrate, Fibre, Fat, Fatty Acids,
Cholesterol, Protein, and Amino Acids (Macronutrients). National Academy Press: Washington DC.
[5a]
Joint FAO/WHO/UNU Expert Consultation on Human Energy Requirements (2004) Human energy requirements: report of a Joint
FAO/WHO/UNU Expert Consultation. (FAO Food and Nutrition Technical Report Series, no. 1) Rome.
[5b]
Joint WHO/FAO/UNU Expert Consultation on Protein and Amino Acid Requirements in Human Nutrition (2007) Protein and amino acid
requirements in human nutrition: report of a joint FAO/WHO/UNU expert consultation. (WHO technical report series; no. 935) Geneva.
[6]
Health Council of the Netherlands (2001) Dietary Reference Intakes: energy, proteins, fats and digestible carbohydrates. The Hague: Health
Council of the Netherlands; publication no. 2001/19R (corrected edition: June 2002).
deficiency in infants in affluent populations, universal requirements between 6 and 24 months of life
iron supplementation appears unjustified. The (Dewey 2001). Animal products are the best source of
ESPGHAN Committee on Nutrition recommends an zinc, both in their content and bioavailability.
iron content ranging from 0.3 to 1.3 mg/100 kcal in
infant formulae (Koletzko et al. 2005). Substantial evi- Calcium
dence indicates that some indictors of iron status, par- Calcium is the most abundant mineral in the human
ticularly ferritin and haemoglobin concentrations, are body (Perez-Lopez 2007). Over 99% of total body
lower in boys than in girls (Domellof et al. 2002). It has calcium is found in teeth and bones. The remainder is
been suggested that boys may have lower body iron present in blood, extracellular fluid, muscle and other
stores at birth or higher intestinal iron losses than girls tissues, where it plays a role in mediating vascular
(Yang et al. 2009). Other possible explanations for the contraction and vasodilatation, muscle contraction,
reported gender difference could be due to genetic or nerve transmission and glandular secretion. The
hormonal factors, but further research is warranted calcium content of infant formulae is often greater
(Domellof et al. 2002). than breast milk to account for a lower fractional
calcium absorption in order to achieve a comparable
Zinc calcium retention (IOM 1997).
Both iron and zinc are critical for normal growth, Iodine
haematopoiesis, immune function and neurologic
development during infancy. The young infant has a Iodine is a trace element required for the production
relatively high zinc requirement to support his very of the thyroid hormones, triiodothyronine and thy-
rapid growth. Zinc and protein intakes have been roxine. These hormones play a vital role in the early
shown to be predictors of head growth (Krebs 2000, growth and development of most organs, especially
2007). Some studies show that zinc intakes are lower the brain (Perez-Lopez 2007). Monitoring of iodine
than recommendations established for this age group, status during infancy is difficult as there are no estab-
but it has been proposed that some current recom- lished reference criteria for urinary iodine concentra-
mendations are higher than actual needs (García- tion for this age group (Zimmermann 2007). The
Ramos Estarriol et al. 2000). iodine content of breast milk and the infant’s small
About 40% of the breast milk zinc content is iodine pool in the thyroid turns over very rapidly and
absorbed, which is considered to be sufficient to meet is highly sensitive to variations in dietary iodine
the requirements of zinc in the first 6 months. To date intake. Studies from France, Germany, Belgium,
there is no marker of zinc deficiency that is sensitive Sweden, Spain, Italy, Denmark, Thailand and Zaire
and specific. This explains why there is considerable have shown that breast milk concentrations of iodine
variability in the requirements and recommendations are lower than recommended levels, suggesting a less
of various international agencies. A committee of than optimal maternal and infant iodine nutrition in
experts convened by the WHO proposed that the many countries (Azizi & Smyth 2009).
recommendations should be based on the metabolic
Sodium
requirements of each age, including a factor that rep-
resents the interference of phytates in the absorption. Infants are less efficient in excreting excess dietary
These recommendations (lower limits of consump- sodium than adults (Michaelsen et al. 2000). High
tion of zinc) were adjusted to diets with low bioavail- sodium intakes during infancy carry both short- and
ability of zinc (phytate content >15 mg/day), median long-term risks. Short-term effects include hyper-
bioavailability (10–15 mg phytate/day) and high bio- natraemia, tissue dehydration, renal dysfunction and
availability (<15 mg phytate/day) (Gil Hernandez increased blood pressure. A possible life threatening
et al. 2006). It has been concluded that complemen- acute complication is severe hypernatriaemic dehy-
tary feeding should provide 84–89% of the zinc dration, which occurs at high sodium intakes without
sufficiently rehydrating a child to compensate for past with 2 mg of copper per litre from 3 months onwards
and ongoing losses, and can lead to renal insufficiency did not show biochemical or clinical evidence of health
and failure to excrete sodium (Fomon 1993). The role problems (Olivares et al. 1998), but the intervention
of salt intake during complementary feeding has not was only started after the age considered most sensi-
been extensively explored. Investigations demon- tive for copper toxicity. An epidemiological survey in
strating that an excess of dietary sodium may raise Germany did not demonstrate a link between high
blood pressure in newborns and young infants have copper exposure from water and evidence of liver
suggested that infancy may be a period of greater salt abnormalities in infants, but there was no systematic
sensitivity than later in life (Agostoni et al. 2008). health assessment for markers of copper toxicity in the
There is evidence that early life factors are important infant population studied (Zietz et al. 2003).
determinants of adult blood pressure (Lawlor & Vitamin A is a group of compounds, the precursor
Smith 2005). The long-term effects of high salt intake of which is trans-retinol. Vitamin A is essential for
in infancy on blood pressure are possible, but so far growth and differentiation of a number of cells and
not proven (Brion et al. 2007). tissues. It is one of the most critical vitamins during
the breastfeeding period because it has an important
role in the healthy development of the newborn, with
Copper
lung development and maturation being particularly
Copper concentrations in human milk decrease during important. Insufficient intake of vitamin A by infants
the course of lactation and appear to be unrelated to may have serious consequences, especially regarding
maternal serum levels (Dorea 2000). Based on the susceptibility to infections, the development and func-
copper concentration of human milk, the Institute of tion of respiratory organs and the integrity of mucous
Medicine (IOM) estimated that intakes of 200 mg/day membranes (Strobel et al. 2007). Vitamin A deficien-
were adequate for the first 6 months of life increasing cies are uncommon in breastfed infants in Europe,
to 220 mg/day for the second 6 months (IOM 2000b). although retinol and retinyl esters content in breast
For formula fed infants, the United States Food and milk varies widely depending on maternal intake.
Drugs Administration (FDA), Codex Alimentarius b-carotene is found in plants and is considered a pro-
and the American Academy of Pediatrics (AAP) rec- vitamin of vitamin A because humans are able to
ommend a minimum level of an artificial formula for convert it into retinol. However, its bioequivalence to
copper of 0.6 mg/kcal, whereas the ESPGHAN Com- retinol in infants is not known. For this reason, the
mittee on Nutrition recommends 35 to 80 mg/100 kcal Scientific Committee on Food of the European Com-
(Koletzko et al. 2005). There has been concern about mission advised that vitamin A activity in infant
the potential risks of excess copper supply in infants formula should be provided by retinol or retinyl
fed powered infant formula mixed with tap water with esters, while any carotene content should not be
a high copper content. Copper in drinking water con- included in the calculation and declaration of vitamin
tributes 0.1–1 mg/day in most situations, but water A activity (SCF 2003).
obtained from unprotected copper pipes or fittings can Vitamin D is required to maintain adequate calcium
considerably increase total daily copper exposure metabolism and bone health at all ages. Many coun-
(WHO 2003a). The WHO has proposed that a copper tries recommend vitamin D supplementation during
content of drinking water not exceeding 2 mg/L-1 infancy to avoid rickets resulting from its low content
would provide an adequate margin of safety in popu- in human milk (Molgaard & Michaelsen 2003). For
lations with normal copper homeostasis (WHO prevention of rickets, the Committee on Nutrition of
2003a); whereas it has been proposed that infants fed the AAP recommends supplementing breastfed
powdered formula prepared with tap water containing infants, whether consuming formula or not, with
>2 mg/L-1 copper may pose a risk for liver damage 400 IU (10 mg) of vitamin D per day from birth
particularly in young infants (Uauy et al. 2008).A con- (Wagner & Greer 2008). In addition to dietary supply,
trolled study of 100 healthy infants consuming water the infant’s vitamin D status is also modified by sun-
light exposure and hence the season of the year and the 2008). Reports of severe neurodevelopmental
geographical latitude. Dark-skinned children or chil- damage and long-term neurologic sequelae due to
dren whose clothing obscures the skin may be at great- infantile cobalamin deficiency demonstrate the
est risk of vitamin D deficiency. Ziegler et al. (2006) importance of adequate cobalamin status during the
reported that 10% of breastfed infants living in Iowa first months of life (von Schenck et al. 1997; Bjorke-
(41 degrees N) were deficient in vitamin D as deter- Monsen et al. 2008). However, reference values for
mined by low serum 25-OH-D levels; most were dark- indices of cobalamin and folate status in infants are
skinned (Ziegler et al. 2006). Sunlight exposure is lacking (Hay et al. 2008).
critical for vitamin D synthesis because cutaneous Several factors may affect dietary intake of micro-
biosynthesis upon exposure of skin to ultraviolet B nutrients such as the composition of foods (e.g. iron
light is the major source of vitamin D for most people, and calcium absorption depends on vitamin C and
which influences vitamin D status and calcium absorp- vitamin D intakes, respectively) (Sandstrom 2001;
tion. Thus, there is an increased reliance on dietary Cashman 2007) and the iodine concentration in the
sources during winter months to help maintain soil that is determined by geographical location
adequate vitamin D status (Cashman 2007; Kimlin (Aston & Brazier 1979).
2008). Nutritional status of infants depends on micronu-
trient supply with human milk that is modified by
Vitamin C maternal micronutrient intake and lifestyle, such us
tobacco smoking, alcohol intake or drug consump-
The biological functions of vitamin C are based on its
tion. Moreover, micronutrient content and bioavail-
ability to provide reducing equivalents for a variety of
ability of infant formula and complementary foods
biochemical reactions (IOM 2000a). Ascorbic acid is
and variability of nutrient absorption are of impor-
important in facilitating iron absorption and a good
tance (IOM 2000a).
ascorbic acid status in the newborn is important for
collagen synthesis and may contribute to protecting
2. Current European nutrient intake
cells against oxidative insults (Jain et al. 2008). Breast-
values for infants
fed infants of well nourished mothers do not require
vitamin C supplementation. Previous EURRECA research activities collated and
compared current micronutrient reference values
Vitamin E from 35 countries and committees, by means of a
questionnaire and background documents (Doets
This vitamin serves as a non-specific chain-breaking
et al. 2008). There follows a summary of the specific
antioxidant and protects cell membranes in the retina
characteristics that concern the infant population
and lungs against oxidant-induced injury (IOM
group, together with a comparison of reference values
2000a). Breast milk concentrations of tocopherol
used in European countries and discussion of the
(~3 IU/100 kcal) appear adequate for meeting infant
methodological approaches to set them.
requirements and particularly high levels are found in
colostrum. A study in the USA observed that infants
2.1 Characteristics of the infant population
and children had low intakes of vitamin E compared
group in currently used micronutrient
with the requirements established by the IOM (IOM
intake values
2000a), but there is some concern that those reference
values may be too high (Briefel et al. 2006). The definition of age groups within infancy, each con-
Folate and cobalamin are necessary cofactors in the sidered as relatively homogenous with regard to
synthesis of RNA and DNA, and cobalamin is nutrient requirement, differ between countries and
required for maintaining the nervous system. Both committees that have set reference values for intake.
vitamins are therefore critical to the rapid growth and This may be due to differences in reasoning in defin-
development during the early years of life (Hay et al. ing population groups, but in most publications these
arguments are not clearly described (Doets et al. between reference values used in Europe that have
2008). Most European countries have set reference been set by the different European countries and
values for one or two age groups during infancy: 13 of European and international committees for each
the 30 countries investigated by Doets and colleagues micronutrient at 3 and 9 months of age (lowest and
and the WHO/FAO defined two age groups, while 12 highest values for each micronutrient, mean value,
countries and the European Commission (EC) 25th and 75th percentile). As reference values are
defined only one age group. Four countries defined expressed as single values, multiple values, ranges and
four different age groups, and one country, the so on, Doets and colleagues defined standardization
Russian Federation, set three age groups. Serbia did procedures to enable comparison (Doets et al. 2008).
not set reference values for infants under the age of 1 Table 4a–d give an overview of the level of refer-
year. The five Nordic countries (Denmark, Finland, ence values set by each country/committee for vita-
Iceland, Norway and Sweden), Croatia, Estonia, Italy mins, minerals and trace elements at two points of
and the EC, do not give values for infants under 6 time (3 and 9 months), defined as ‘high level’ when
months. France did not set reference values for min- they exceed the 75th percentile or ‘low level’ when
erals under 1 year of age, but they did for vitamins. they fall below the 25th percentile.
None of the countries or committees set different Twenty-two countries,WHO/FAO and the EC have
values for male and female infants. their own background reports on the setting of nutri-
Specific subpopulation groups within the infant ent reference values; 13 countries based their refer-
group are specified for particular micronutrients by ence values on those of other countries or
some countries. Groups recognized are bottle and organizations; Germany, Austria and Switzerland
formula fed infants, respectively (the Netherlands: (DACH) and the Nordic countries cooperated in
calcium, phosphorus, zinc and vitamin B6; WHO/ setting reference values. The Nordic countries, the
FAO: zinc and magnesium), and cows’ milk fed DACH countries, France, Latvia, the Netherlands and
infants (WHO/FAO: calcium). the United Kingdom, WHO/FAO and the EC, defined
The infant population group is one of the popula- their own reference values (Doets et al. 2008). Table 5
tions recognized as a ‘vulnerable’ group (defined as a gives an overview of the European countries and
population group in a healthy population having a international working groups with published micro-
higher nutrient requirement) by 8 of the 35 countries nutrient reference values used in Europe. From those
and committees questioned in the scoping exercise countries and committees setting their own reference
for collecting reference values (Doets et al. 2008). The values, only a small number of documents provided
most commonly cited micronutrients thought to be detailed information of the evaluation of evidence
related to infant vulnerability were vitamin D (in supporting the reference values (United Kingdom
three countries), vitamin A (n = 2), iodine (n = 2), 1991; EC 1993; DACH 2000; the Netherlands 1992,
copper (n = 2) and iron (n = 2). 2000 and 2003; France 2001; NNR 2004; WHO/FAO
For the purposes of EURRECA, a list of terms and 2004). We will solely refer to these documents when
definitions that are relevant to the infancy period and examining the methodological approaches for setting
to infant feeding have been agreed for use within the their micronutrient reference values in detail (see
network (Table 2). tables in section 2.2.1 to 2.2.4).
2.2.1 Water soluble vitamins (Table 6)

2.2 Comparison of nutrient intake values
for infants
2.2.2 Fat soluble vitamins (Table 7)
Micronutrient reference values for infants vary con-
siderably across Europe; for some micronutrients 2.2.3 Minerals (Table 8)
there is a great difference between the highest and the
lowest values. Table 3a and b show the differences 2.2.4 Trace elements (Table 9)
Table 2. List of terms and definitions related to the infant population group and adopted by EURRECA Research Activity ‘infant nutrition’
Term Definition
Infant A person in the first year of life.

The first 12 months of life have been defined as an age group to set reference values by national and
international committees. Within this age group, reference values are set for one to four different age
subgroups. Most European countries defined one or two age subgroups.
For the purposes of EURRECA, the infant group is defined from birth up to 12 months of age.
Young infant An infant up to the 6th month of life (completed 180 days).
Older infant An infant from about the 6th month of life up to 1 year of age.
Newborn An infant from birth to 28 completed days.
The term newborn includes premature infants, post-mature infants and full term newborns.[1]
Pre-term birth Delivery before 37 completed weeks of gestation.[1]
Full-term Infant born between 37 completed weeks of gestation and less than 42 weeks.
Post-mature neonate Infant born after 42 weeks gestation.
Gestational age Time elapsed between the first day of the last menstrual period and the day of delivery. Measured in
completed weeks. [Note: If pregnancy was achieved using assisted reproductive technology, is
calculated by adding 2 weeks to conceptional age (time elapsed after conception)].[2]
Chronological age Time elapsed since birth. Measured in days, weeks, months or years.[2]
Post-menstrual age Gestational age + chronological age. Measured in days, weeks, months or years.[2]
Corrected age (similar term: adjusted age) Chronological age reduced by the number of weeks born before 40 weeks of gestation. Measured in
weeks or months used for preterm children until 3 years old.[2]
Toddler Young child who is of the age of learning to walk between infancy and childhood. Toddling usually
begins between age 12 and 24 months
Low birthweight (LBW) infant A neonate weighing less than 2500 g at birth (up to and including 2499 g), irrespective the gestational
age.[1]
Very low birthweight (VLBW) infant A neonate weighing less than 1500 g at birth.[1]
Extremely low birthweight (EXLW) A neonate weighing less than 1000 g at birth.[1]
Small for gestational age (SGA) infant Neonates with birthweight and/or length at least 2 standard deviations (SDs) below the mean for
gestational age (ⱕ-2 SD) based on the data derived from a reference population.[3]
Exclusive breast-feeding Exclusive breast-feeding implies that the infant receives only breast milk (including milk expressed or
from a wet nurse) and no other liquids or solids except for Oral Rehydration Solution (ORS) drops or
syrups consisting of vitamins, mineral supplements, or medicines.[4]
Predominant breastfeeding Predominant breastfeeding implies that breast milk (including milk expressed or from a wet nurse) is the
predominant source of nourishment, in combination with the supply of certain liquids (water and
water-based drinks, fruit juice) ritual fluids and ORS, drops or syrups (vitamins, mineral, medicines).[4]
Full breastfeeding Exclusive breastfeeding and predominant breastfeeding together.[5]
Breastfeeding Breastfeeding implies that the infant receives breast milk (including milk expressed or from a wet nurse)
and can also receive any food or liquid including non-human milk and formula.[4]
No breastfeeding The infant receives no breast milk.[5]
Complementary feeding (similar terms: The term ‘complementary feeding’ should embrace all solid and liquid foods other than breast milk or
weaning, weaning infant formula and follow-on formula.[6]
foods, Beikost)
Bottle-feeding Any liquid (including breast milk) or semi-solid food from a bottle with nipple/teat.[4]
Infant formulae Foodstuffs intended for particular nutritional use by infants during the first months of life and satisfying
by themselves the nutritional requirements of such infants until the introduction of appropriate
complementary feeding.[7]
Follow-on formulae Foodstuffs intended for particular nutritional use by infants when appropriate complementary feeding is
introduced and constituting the principal liquid element in a progressively diversified diet of such
infants.[7]
Breast milk substitutes Any food being marketed or otherwise presented as a partial or total replacement for breast milk,
whether or not suitable for that purpose.[8]
[1]WHOSIS (WHO statistical information system).

[2]American Academy of Pediatrics. Age Terminology During the Perinatal Period. Pediatrics 2004;114:1362–1364.
[3]International Small for Gestational Age Advisory Board consensus. Development conference statement: management of short children born small for gestational age,
April 24–October 1, 2001. Pediatrics. 2003 June;111(6 Pt 1):1253–61.

[4]Indicators for assessing infant and young child feeding practices: conclusions of a consensus meeting held 6–8 November 2007 in Washington D.C., USA. WHO 2008.
[5]
Indicators for assessing breastfeeding practices. Report of an informal meeting in June 1991, Geneva. World Health Organization, Geneva.
[6]Agostoni et al. Complementary Feeding: A Commentary by the ESPGHAN Committee on Nutrition Journal of Pediatric Gastroenterology and Nutrition 2008,
46:99–110.
[7]
Commission Directive 2006/141/EC of 22 December 2006 on infant formulae and follow-on formulae and amending Directive 1999/21/EC.
[8]International Code of Marketing of Breast-milk Substitutes. World Health Organization Geneva, 1981.
Table 3. Comparison of micronutrient recommendations for infants at 3 and 9 months of age
Micronutrient (Unit) Lowest reference value Highest reference value Median P25† P75‡
a. Comparison of micronutrient recommendations for infants at 3 months of age

Thiamin (mg) 0.2 0.5 0.3 0.2 0.3
Rivoflavin (mg) 0.3 0.6 0.4 0.4 0.4
Niacin (mg) 2 8 4 2.3 5
Vitamin B12 (mg) 0.3 0.5 0.4 0.3 0.5
Folate (mg) 24 80 50 40 64
Vitamin C (mg) 25 50 35 29 40
Vitamin B6 (mg) 0.1 0.5 0.3 0.2 0.3
Vitamin A (mg) 350 500 400 375 435
Vitamin D (mg) 5 22.5 10 6.3 10
Vitamin E (mg) 2.7 6 3 3 4
Calcium (mg) 210 800 400 360 500
Phosphorus (mg) 100 400 300 141 300
Potassium (mg) 233 800 600 400 800
Sodium (mg) 100 638 210 200 250
Magnesium (mg) 24 70 50 39 55
Iodine (mg) 15 110 40 40 50
Iron (mg) 0.3 12.5 5 1.7 7
Zinc (mg) 1 5 3,5 2 4
Selenium (mg) 6 25 10 10 10
Copper (mg) 0.2 1.5 0.4 0.2 0.5
b. Comparison of micronutrient recommendations for infants at 9 months of age
Thiamin (mg) 0.2 0.6 0.4 0.3 0.4
Rivoflavin (mg) 0.4 0.7 0.4 0.4 0.5
Niacin (mg) 2 8 5 4 6
Vitamin B12 (mg) 0.3 1.5 0.5 0.5 0.5
Folate (mg) 32 80 50 50 66
Vitamin C (mg) 20 55 35 30 40
Vitamin B6 (mg) 0.2 0.6 0.4 0.3 0.6
Vitamin A (mg) 300 600 388 350 400
Vitamin D (mg) 5 22.5 10 7 10
Vitamin E (mg) 2.7 6 4 3 4
Calcium (mg) 270 800 540 438 600
Phosphorus (mg) 200 500 400 300 500
Potassium (mg) 425 1110 750 700 800
Sodium (mg) 180 850 370 320 500
Magnesium (mg) 48 100 70 60 75
Iodine (mg) 40 135 50 50 63
Iron (mg) 6 15 9 7.8 10
Zinc (mg) 2 5 4.5 3.8 5
Selenium (mg) 8 40 15 10 19.3
Copper (mg) 0.2 1.5 0.4 0.3 0.7
†
25th percentile; ‡75th percentile.
68
Table 4. Overview of the level of reference values between countries for vitamins, minerals and trace elements
M. Hermoso et al.
Country Year Ref. Vitamin C Vitamin B6 Vitamin A Vitamin D Vitamin E
3 months 9 months 3 months 9 months 3 months 9 months 3 months 9 months 3 months 9 months
a. Overview of the level of reference values between countries for vitamins
Albania 2005 [1] High High Low Low High

EC 1993 [2] – Low – – – High – –
Croatia 2004 [3] – – – – –
the Netherlands 1992 [4a] High Low Low – –
2000 [4b]
2003 [4c]
2008# [4d]
Ireland 1999 [5] Low Low Low – –
DACH 2000 [6] High High High High
Belgium 2006 [7] High Low High High – –
Romania 1990 [8] High High High – –
Latvia 2001 [9]
France 2001 [10] High High Low High High
The former Yugoslav Republic 2001 [11]
of Macedonia
Slovakia 1997 [12] High High High High
Lithuania 1999 [13]
Bulgaria 2005 [14] Low Low Low Low Low
Nordic countries 2005 [15] – Low – – Low – –
United Kingdom 1991 [16] Low Low Low – –
Bosnia and Herzegovina 2005 [17] Low Low Low Low Low Low
Spain 2007 [18] High High High High High High
Serbia 1994 [19] – – – – – – – – –
Italy 1996 [20] – – – – High – –
Russian Federation 1991 [21] High
Estonia 2006 [22] – – – Low – –
WHO/FAO 2004 [23] Low Low Low Low Low
Poland 1996 [24] High High High
Iceland 2006 [25] – Low – – Low – –
Hungary 2005 [26] High High
b. Overview of the level of recommendations between countries for vitamins
Thiamin Riboflavin Niacin Vitamin B12 Folate
Albania 2005 [1] Low High High

EC 1993 [2] – – – – –
Croatia 2004 [3] – – – – – Low
the Netherlands 2000 [4b] Low Low
2003 [4c]
Ireland 1999 [5] – –
DACH 2000 [6] Low High High
Belgium 2006 [7] High High
Romania 1990 [8] High High High – – – –
Latvia 2001 [9] High High Low Low
France 2001 [10] Low High High
The former Yugoslav Republic 2001 [11] High Low Low
of Macedonia
Slovakia 1997 [12] High High
Lithuania 1999 [13] High High High High Low Low
Bulgaria 2005 [14] Low High High
Nordic countries 2005 [15] – – – – –
United Kingdom 1991 [16]
Bosnia and Herzegovina 2005 [17] Low High High
Spain progr [18] High Low
Serbia 1994 [19] – – – – – – – – –
Italy 1996 [20] – – – –
Russian Federation 1991 [21] High High High
Estonia 2006 [22] – – – – –
WHO/FAO 2004 [23] Low High High High
Poland 1996 [24] High High High High High High Low
Iceland 2006 [25] – – – – –
Hungary 2005 [26]
Nutritional requirements of infants
69
70
Table 4. Continued
M. Hermoso et al.
Country Year Ref. Vitamin C Vitamin B6 Vitamin A Vitamin D Vitamin E
c. Overview of the level of recommendations between countries for minerals
Calcium Phosphorus Potassium Sodium Magnesium
Albania 2005 [1] Low Low Low Low Low Low

EC 1993 [2] – Low – – – – – –
Croatia 2004 [3] – – – – – – –
the Netherlands 1992 [4a] Low – Low – – – – Low
2000 [4b]
Ireland 1999 [5] High – –
DACH 2000 [6] Low Low Low Low Low Low Low
Belgium 2006 [7] – – – –
Romania 1990 [8] High High High – – – – High
Latvia 2001 [9]
France 2001 [10] – – – – – – – – –
The former Yugoslav Republic 2001 [11] High High
of Macedonia
Slovakia 1997 [12] – – – – High
Lithuania 1999 [13] – – – –
Bulgaria 2005 [14] Low Low Low Low – – – – Low Low
Nordic countries 2005 [15] – – – High – – – High
United Kingdom 1991 [16] High High
Bosnia and Herzegovina 2005 [17] Low – – High Low Low
Spain progr [18] Low Low – – High High
Serbia 1994 [19] – – – – – – – –
Italy 1996 [20] – – – – –
Russian Federation 1991 [21] – –
Estonia 2006 [22] – – – High – High
WHO/FAO 2004 [23] Low (cow milk) Low – – – – Low Low
Poland 1996 [24] High High Low Low High High
Iceland 2006 [25] – – – High – High
Hungary 2005 [26] Low
d. Overview of the level of recommendations between countries for trace elements
Iodine Iron Zinc Selenium Copper
Albania 2005 [1] High High Low High Low High High –
EC 1993 [2] – – Low – – Low –
Croatia 2004 [3] – – – –
the Netherlands 1992 [4a] – – Low Low High

Ireland 1999 [5]
DACH 2000 [6] High (no Low Low Low High
Switz)
Belgium 2006 [7] High High –
Romania 1990 [8] Low Low – – – –
Latvia 2001 [9] High
France 2001 [10] – – – – – – – – – –
The former Yugoslav Republic 2001 [11] High High High High High
of Macedonia
Slovakia 1997 [12] High High
Lithuania 1999 [13] Low – – – –
Bulgaria 2005 [14] High High Low High Low
Nordic countries 2005 [15] – – – – –
United Kingdom 1991 [16]
Bosnia and Herzegovina 2005 [17] Low High High High Low Low Low High High
Spain progr [18] Low Low Low – –
Serbia 1994 [19] – – – – – – – – – –
Italy 1996 [20] – Low – – Low –
Russian Federation 1991 [21] – –
Estonia 2006 [22] – – – – –
WHO/FAO 2004 [23] High High – High Low –
Poland 1996 [24] High High High
Iceland 2006 [25] – – – –
–
Hungary 2005 [26]
‘High’ means reference value is above the 75th percentile; ‘low’ means reference value is below the 25th percentile. Blank cells indicate that the reference value is between the 25th and 75th
percentile.
71
Table 5. List of European countries and international working groups with published reference values for vitamins and minerals.
Albania 2005 [1] Adopted from the literature (especially Linus Pauling Institute)
European Community (EC) 1993 [2]* Own
Croatia 2004 [3] Aligned with EU legislation
the Netherlands 1992, 2000, 2003 [4a] [4b] [4c] Own
Ireland 1999 [5] Adopted from EC and United Kingdom. Own recommendations for calcium,
iron (and folate and vitamin C)
Germany, Austria, Switzerland 2000 [6]* Own
Belgium 2006 [7]** Based on WHO/FAO, EC and European countries culturally and geographically
related to Belgium (United Kingdom, the Netherlands, France)
Romania 1990 [8] Own
Latvia 2001 [9] Own
France 2001 [10] Own
The former Yugoslav Republic of Macedonia 2001 [11] Based on WHO/FAO and United Kingdom
Slovakia 1997 [12] Adopted from unknown source
Lithuania 1999 [13] Own
Bulgaria 2005 [14] Based on WHO/FAO and IOM
Denmark, Finland, Norway, Sweden 2005 [15]* Own
United Kingdom 1991 [16]* Own
Bosnia and Herzegovina, entity: Republic of Srpska 2005 [17] Adopted from unknown source
Spain [18] Own
Serbia 1994 [19] Adopted from unknown source
Italy 1996 [20] Based on EC and FNB
Russian Federation 1991 [21] Own
Estonia 2006 [22] Based on Nordic Council of Ministers
WHO/FAO 2004 [23]* Own
Poland 1996 [24]** Based on United Kingdom, EC and FNB
Iceland 2006 [25] Shared with Nordic countries. Own recommendations on calcium and vitamin D
Hungary 2005 [26] Based on EC and IOM
*Background scientific report available in English language; **Polish and Belgian reference values have been revised in 2008.
[1] Berisha A., Bader E., Deligia C and Claude Dop M. (2005) Nutrition country profile Republic of Albania.
[2] Commission of the European Communities (1993) Nutrient and energy intakes for the European Community. Reports of the Scientific Committee for Food. Thirty
first series.
[3] Croatian National Official Gazzette 81 (2004).
[4a] Food and Nutrition Council (1992) Dutch dietary reference values 1989 (in Dutch: Nederlandse normen 1989), 2nd edn. The Hague.
[4b] Health Council of the Netherlands (2000) Dietary Reference Values: calcium, vitamin D, thiamine, riboflavin, niacin, pantothenic acid, and biotin. The Hague: Health
Council of the Netherlands; publication no. 2000/12.
[4c] Health Council of the Netherlands (2003) Dietary Reference Intakes: vitamin B6, folic acid, and vitamin B12. The Hague: Health Council of the Netherlands;
publication no. 2003/04.
[4d] Health Council of the Netherlands (2008) Towards an adequate intake of vitamin D. The Hague: Health Council of the Netherlands; publication no. 2008/15E.
[5] Food Safety Authority of Ireland (1999) Recommended Dietary Allowances for Ireland, Dublin.
[6] German Nutrition Society (DGE), Austrian Nutrition Society (OGE), Swiss Society for Nutrition Research (SGE), Swiss Nutrition Association (SVE) (2000)
Referenzwerte für die Nahrstoffzufuhr/Reference Values for Nutrient Intake, 1st edition in German. Frankfurt/Main.
[7] Hoge Gezondheidsraad (2006) Voedingsaanbevelingen voor Belgie, herziening.
[8] Institute of Public Health (1990) Recommended amounts. Romania.
[9] Latvian Food Center (2001) Leteicamas energijas un uzturvielu devas Latvijas iedzivotajiem/recommended intake of energy and nutrients for Latvians.
[10] Martin A. (Guest Editor-in-Chief) (2001). Nutritional recommendations for the Grenc population. Sciences des aliments. International Journal of Food Science and
Technology 21, 309–438.
[11] Ministry of Health and Republic Institute of Health Protection (2001). Nutrient recommendations as addition to Guidelines for Healthy nutrition of the population
in Republic of Macedonia.
[12] Jajaba I., Simoncic R., Ginter E., Ondrejka J., Trusková I., Kalác J., Bzdúch V. (1997) Odporúcané výzivové dávky pre obyvateltvo Slovenska (8. revízia OVD). Výz.
Zdravie, 4, 2:25–29. Official Journal of Ministry of Health.28, part 7–8 Slovakia.
[13] Lithuanian Ministry of Health, National Nutrition Center (order no. 510) (1999). Recommended dietary allowances for Lithuanian population (energy, protein, fat,
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Table 6. Comparison of reference values for water soluble vitamins for infants and methods used to estimate requirements
Range between countries Method on which the estimated requirements is based
3m 9m Younger infants Older infants
Method Country Method Country
Thiamine 0.2–0.5 mg/d 0.2–0.6 mg/d Adequate intake from DACH, WHO/FAO, Adequate intake from human milk DACH, WHO/
human milk United Kingdom, FAO, United
the Netherlands Kingdom
Interpolated between values for young infants and for adults the Netherlands
Derived from adult values on the basis of energy expenditure EC, NNR
Riboflavin 0.3–0.6 mg/d 0.4–0.7 mg/d Adequate intake from DACH, WHO/FAO, Adequate intakes from human milk DACH, WHO/
human milk the Netherlands FAO
Intake related to a United Kingdom Necessary intake to restore the erythrocyte glutathione reductase activation EC, United
satisfactory coefficient Kingdom
riboflavin status Derived from adult values on the basis of energy expenditure NNR
using erythrocyte Interpolated between values for young infants and for adults the Netherlands
glutathione
reductase activation
coefficient according
to a study on
Gambian infants
(Bates et al. 1982)
Niacin 2.0–8.0 mg/d 2.0–8.0 mg/d Adequate intake from DACH, WHO/FAO, Derived from adult values on the basis of energy expenditure United Kingdom,
human milk the Netherlands EC, NNR
VitB12 0.3–0.5 mg/d 0.3–1.5 mg/d Adequate intake from DACH, WHO/FAO, Adequate intake from human milk WHO/FAO
human milk the Netherlands
Intake required to United Kingdom Intake seen to correct biochemical deficiency as evidenced by methylmalonic EC, United
normalize methyl acid excretion Kingdom, NNR
malonic acid Interpolated from young infants on the basis of weight increase DACH
excretion according Or interpolated between values for young infants and for adults the Netherlands
to a study carried
out in infants of
vegan mothers
(Specker et al 1990)
73
74
Table 6. Continued
M. Hermoso et al.
Range between countries Method on which the estimated requirements is based
Folate 24–80 mg/d 32–80 mg/d Adequate folate DACH, WHO/FAO, Adequate intake from human milk WHO/FAO
intakes from human United Kingdom, Interpolated from young infants on the basis of weight increase DACH
milk the Netherlands Interpolated between values for young infants and for adults the Netherlands
Based on an intake of 3.6 mcg/kg body weight appeared to maintain plasma NNR, EC
levels and showed maintained growth, heamopoiesis and clinical well being
of the 20 infants involved in a trial (Asfour et al. 1977)
Set a value for formula fed infants based on the adequate intake from human United Kingdom
milk plus intake from formula that produced lower red cell folate levels
than breast milk does but no differences in haemoglobin concentration,
weight gain or growth rate according to a supplementation study in infants
(Foged et al. 1989)
Vitamin C 25–50 mg/d 20–55 mg/d Based on vitamin C DACH, WHO/FAO, Based on vitamin C content of breast milk considering maternal intake DACH, EC and
content of breast United Kingdom, United Kingdom
milk considering the Netherlands, FR From the reference value estimated for young infants WHO/FAO
maternal intake Extrapolated from adult requirements by assuming a growth factor of 1.3 NNR
From those for adults based on square heights France
Vitamin B6 0.1–0.5 mg/d 0.2–0.6 mg/d Usual vitamin B6 DACH, WHO/FAO, Usual intakes from human milk DACH, United
intakes from human United Kingdom, Kingdom
milk the Netherlands Derived from adult values on the basis of dietary protein intake, assuming DACH, United
that 15% of energy is provided from protein as for adult values and then Kingdom
using values set for energy expenditures
Extrapolated from values for young infants on the basis of metabolic size, EC
weight and growth, considering vitamin B6 content of breast milk, with
consideration of average maternal intakes and average plasma pyridoxal
phosphate (PLP) of nursing infants
Extrapolated values for adults using an adjustment for body height France
Table 7. Comparison of reference values for fat soluble vitamins for infants and methods used to estimate requirements
Range between countries Method on which estimated requirements is based
Vitamin A 350–500 mcg/d 300–600 mcg/d Usual intakes from breast milk DACH, WHO/FAO, the Based on usual intakes from DACH
Netherlands breast milk
Intakes considered sufficient to United Kingdom Extrapolated from adult NNR
build and maintain sufficient requirements by using metabolic
liver stores body weight and growth factors
Considered sufficient to build and WHO/FAO, United Kingdom, EC
maintain sufficient liver stores
Vitamin D 5–22.5 mcg/d 5–22.5 mcg/d Vitamin D intakes sufficient to DACH, WHO/FAO, United Vitamin D intakes sufficient to DACH, WHO/FAO, United
maintain plasma 25-OHD levels Kingdom, the Netherlands maintain plasma 25-OHD levels Kingdom, EC, the Netherlands
within a range considered to within a range considered to
support adequate bone health support adequate bone health
Took into account additional NNR
intakes showing to have
maximal effect in linear growth
according to supplementation
studies in infants
Vitamin E 2.7–6 mg/d 2.7–6 mg/d Based on vitamin E content of Derived from adult values on an France
breast milk energy allowance basis
Consider vitamin E intake in
relation to PUFA intake
75
76
Table 8. Comparison of reference values for minerals for infants and methods used to estimate requirements

M. Hermoso et al.
Calcium 210–800 mg/d 270–800 mg/d Usual calcium intakes from breast milk the Netherlands, Based on usual intake from formula or food DACH
DACH, France
Based on calcium retention (factorial method for WHO/FAO, United Based on calcium retention the Netherlands,
calcium needed for growth, including skeleton, Kingdom WHO/FAO, NNR,
urinary excretion and insensible losses) followed by EC, United
absorption studies Kingdom
Phosphorus 100–400 mg/d 200–500 mg/d Based on breast milk content DACH, France Based on the infant formulae content DACH
On the basic principle of an equimolar relationship in United Kingdom On the equimolar relationship with calcium the Netherlands,
the body between calcium and phosphorus United Kingdom,
EC, NNR
The minimum level based on a relation of Ca/P of 1.7 the Netherlands On the amount required for growth derived from the France
molar, to lower the risk on tetany values for calcium with the Ca/P ratio for weight
gain of about 1.7 molar
Potassium† 233–800 mg/d 425–1100 mg/d Based on the need to maintain electrolyte homeostasis DACH United Factorial estimation United Kingdom,
and for growth of cellular mass based on factorial Kingdom EC
estimation of the urinary excretion, the amount
needed for growth and lean tissue synthesis, the Extrapolated values from adult requirements by using NNR
integumental and faecal losses (although the latter metabolic body weight and growth factors (the
may represent homeostatic excretion of excessive values of the adults are set by considering the effect
intakes or losses incurred in maintaining sodium on blood pressure)
homeostasis)
Sodium‡ 100–638 mg/d 180–850 mg/d Based on the requirement for maintenance and DACH Based on the requirement for maintenance and DACH
growth, according to balance studies and body growth, according to balance studies and body
analysis analysis
On breast milk content of sodium United Kingdom On the daily increments in total sodium body content United Kingdom
allowing for the declining proportion with age of
extracellular fluid in body mass with an allowance
for dermal, faecal and urinary losses
Magnesium§ 24–70 mg/d 48–100 mg/d On the basis of magnesium usual intake from breast DACH, WHO/FAO, On the basis of magnesium usual intake from breast DACH, WHO/FAO,
milk United Kingdom, milk NNR, United
France Kingdom, France
Extrapolated from the19–21 year age group, whose the Netherlands
values are estimated on the basis of balance studies
and taking the body weight into account
†Out of 26 countries, only 14 have recommendations for potassium (Albania, EC, Ireland, DACH, Latvia, the former Yugoslav Republic of Macedonia, Nordic countries, United Kingdom, Spain, Italy, Estonia, Poland, Iceland,
Hungary).
‡
Out of 26 countries, only 9 (Albania, Ireland, DACH, Latvia, the former Yugoslav Republic of Macedonia, United Kingdom, Bosnia and Herzegovina, Poland, Hungary) have recommendations for sodium for infants.
§According to WHO/FAO, the magnesium in human milk is absorbed with substantially greater efficiency (about 80–90%) than that of formula milks (about 55–75%) or solid foods (about 50%).
Table 9. Comparison of reference values for trace elements for infants and methods used to estimate requirements
Iodine 15–110 mg/d 40–135 mg/d Based on the iodine concentration in breast DACH, WHO/FAO, Balance studies were used to estimate the WHO/FAO
milk, which depends on the mother’s iodine United Kingdom adequate mother’s intake, and these are
intake valid also for old infants
Balance studies were used to estimate the WHO/FAO Extrapolated from adults aged 19–24 y, DACH†
adequate mother’s intake, and these are considering the iodine status in balance
valid also for old infants studies, the connection between iodine
status and goitre frequency in
epidemiological studies, the relation
between long-term iodine intake and iodine
content of the thyroid gland, and
inactivation of active autonomous thyroid
adaptation mechanisms once a certain level
of iodine intake has been exceeded.
Derived values for older infants from adult EC
values, making a rough estimate on the
basis of energy requirements and body
weight
Based on data on goitre prevalence and NNR
urinary iodine excretion in European
children and extrapolation from adult
values based on energy and growth
requirement
Extrapolated from adult values using EARs United Kingdom
for energy, which being based on basal
metabolic rate have a weight component
Iron‡ 0.3–12.5 mg/d 6–15 mg/d A requirement for dietary iron exists only DACH, WHO/FAO Based on iron requirements for growth and DACH, NNR,
from the 4th month due to the newborn’s on iron basal losses United Kingdom
reserve of placental iron (Hb iron)
Extrapolation based on metabolic weight the Netherlands Extrapolation based on metabolic weight the Netherlands
Based on the amount of body stores of iron WHO/FAO
and by the properties of the diet (iron
content and bioavailability)
Considers body weight and energy EC
expenditure
Zinc 1–5 mg/d 2–5 mg/d Based on zinc content in breast milk DACH Based on zinc content in breast milk DACH
Extrapolation from adult values of losses and United Kingdom, Extrapolation from adult values of losses and United Kingdom,
need for growth estimated with factorial the Netherlands, need for growth estimated with factorial the Netherlands,
methods and balance studies WHO/FAO methods and balance studies WHO/FAO
Based on metabolic weight the Netherlands Based on metabolic weight the Netherlands
Basal metabolic rates and considering 3 WHO/FAO Basal metabolic rates and considering 3 WHO/FAO
different bioavailabilities different bioavailabilities
Factorial method EC, NNR

77
78
M. Hermoso et al.
Table 9. Continued
Selenium 6–25 mg/d 8–40 mg/d During the first months of life low selenium the Netherlands, It valid also for older infants, with an United Kingdom
intake is sufficient as selenium has been DACH allowance for growth taken into account
stored in the liver before birth; then,
recommendations for younger infants are
based on breast milk content of selenium
Interpolation of the estimates for 65 Kg adult WHO/FAO Interpolation of the estimates for 65 Kg adult WHO/FAO
males of average normative males of average normative
requirement + 2SD deviation assumed of requirement + 2SD deviation assumed of
12.5%; nonetheless these values are 12.5%; nonetheless these values are
compatible with estimates of the selenium compatible with estimates of the selenium
intake from breast milk and formula intake from breast milk and formula
Based on extrapolation from adult values of DACH, the
the values of plasma glutathione peroxidase Netherlands, EC,
activity on the basis of body weight NNR, WHO/FAO
Copper 0.2–1.5 mg/d 0.2–1.5 mg/d Based on the amount of copper in breast the Netherlands, Extrapolated from adult levels based on body the Netherlands,
milk, due to fetal copper stores in the liver DACH, United weight NNR
and to the high absorption rate Kingdom, France
Based on the copper tissue content and an United Kingdom,
adjustment for loss of endogenous copper, EC
assuming an absorption of 50%
Based on the liver stores France
†
Germany/Austria and Switzerland adopted two different values: Switzerland adopted those from the former WHO/FAO.
‡
Different bioavailabilities (EC, UK: 15%), WHO/FAO’s value is the average of 4 different bioavailabilities, DACH does not take into account bioavailability.
3. Concluding remarks contexts, gender relationships, food availability, local

cultural practices, lifestyles, attitudes and beliefs are
The summary in section 2 reflects great disparities in known to play a role in child health and nutrition
the established current micronutrient reference (Pelto 1987). Poverty may be used as an example of
values for infants between European countries. Envi- such a factor,for example in some Western countries
ronmental and life-style factors can justify national or the lower the socio-economic group of the parents the
regional differences only to a limited extent. In most less likely the woman is to breastfeed or give comple-
cases, the large variation of reference values is the mentary foods at the appropriate time (e.g. Ponza
result of different methodological approaches used to et al. 2004; Heinig et al. 2006; Bolling et al. 2007;
establish requirements and derived NIVs. This is illus- Schiess et al. 2010), for a wide range of socio-cultural
trated in section 2 by the comparison of approaches reasons and embedded cultural norms (Sellen 2001;
followed by seven committees to establish reference Dykes 2005a,b, 2006; Spiro 2006; Scavenius et al. 2007;
values based on their published scientific reports. Bhutta et al. 2008), placing the infant under consider-
Further discrepancies are found when converting able nutritional disadvantage. Low income mothers
requirements to intake reference values on the basis and their infants are also more likely to eat an
of body weight or of energy intake, for which the ‘unhealthy’ diet (high in fat, sugar and salt and low in
selected normative data used are often not explained. fruit and vegetables) than those on a higher income
Similarly, different extrapolation methods used result (Attree 2005). Despite various initiatives aimed at
in different reference values. Furthermore, up to four improving diet in poor households, evidence suggests
different age groups are defined within the first year they have limited effectiveness among lower socio-
of life within which reference values are set. Other economic groups who are their prime target (Lynch
than the transition from milk to complementary et al. 2007). Instead interventions should be multi-
foods, no clear explanations are given for the estab- faceted and include measures to improve families’
lishment of reference values for different age groups socio-economic circumstances (Attree 2005). It is
in the first 12 months of life. therefore essential to recognize any socio-cultural,
Thus, a major issue in understanding the existing political and economic constraints upon women and
disparities of reference values is the lack of transpar- families in securing optimum nutritional standards for
ency in the documentation of the decision making by their infants.
the committees in charge. As an example, only 7 of 30
committees have published scientific reports that we
Acknowledgments
could use for comparing the approaches used to
establish reference values. Even in the available The authors are grateful to all EURRECA members
reports, clear explanations on assumptions, decision and partners involved in data collation, entry, analyses
criteria and reference data are often lacking. Improv- of current recommendations Liesbeth S. de Wit,
ing transparency in the process of setting micronutri- Rosalie A.M. Dhonukshe-Rutten, Adriënne E.J.M.
ent reference values has major implications for Cavelaars, Anna Brzozowska Trudy M.A. Wijnhoven,
nutritional policy (e.g. development of nutritional Mirjana Pavlovic, Torunn Holm Totland, Lene F.
guidelines for infant feeding and regulation of infant Andersen, Jiri Ruprich, Lisette C.P.G.M. de Groot,
formula and follow-on formula composition), and Pieter van ‘t Veer.
feeding practice.
Reference values need to be translated in nutri-
Source of funding
tional guidelines. When attempting to apply any
future guidelines to the infant population group, The work report herein has been carried out within
attention should also be placed upon the social, cul- the EURRECA Network of Excellence (http://
tural and structural determinants of infant feeding www.eurreca.org), which is financially supported by
practices. Factors such as socio-economic and political the Commission of the European Communities, spe-
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The Nutritional Requirements of Infants. Towards EU Alignment of Reference Values: The EURRECA Network

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DOI: 10.1111/j.1740-8709.2010.00262.

Maria Hermoso*, Garden Tabacchi†, Iris Iglesia-Altaba‡, Silvia Bel-Serrat‡,

Background reference values for intake (Doets et al. 2008). Infants

1. Nutritional requirements of and experienced less morbidity from gastrointestinal

Age Energy Protein Fat Essential fatty acids

Male/female Male/female Male/female n-6 n-3

DACH[1] Month kcal/d g/d % of energy % of energy

2.2.1 Water soluble vitamins (Table 6)

Infant A person in the first year of life.

[1]WHOSIS (WHO statistical information system).

April 24–October 1, 2001. Pediatrics. 2003 June;111(6 Pt 1):1253–61.

Table 3. Comparison of micronutrient recommendations for infants at 3 and 9 months of age

a. Comparison of micronutrient recommendations for infants at 3 months of age

Country Year Ref. Vitamin C Vitamin B6 Vitamin A Vitamin D Vitamin E

a. Overview of the level of reference values between countries for vitamins

Albania 2005 [1] High High Low Low High

Thiamin Riboflavin Niacin Vitamin B12 Folate

Albania 2005 [1] Low High High

Country Year Ref. Vitamin C Vitamin B6 Vitamin A Vitamin D Vitamin E

c. Overview of the level of recommendations between countries for minerals

Calcium Phosphorus Potassium Sodium Magnesium

Albania 2005 [1] Low Low Low Low Low Low

Iodine Iron Zinc Selenium Copper

the Netherlands 1992 [4a] – – Low Low High

Range between countries Method on which the estimated requirements is based

3m 9m Younger infants Older infants

Method Country Method Country

Range between countries Method on which the estimated requirements is based

3m 9m Younger infants Older infants

Method Country Method Country

Range between countries Method on which estimated requirements is based

3m 9m Younger infants Older infants

Method Country Method Country

Range between countries Method on which estimated requirements is based

3m 9m Younger infants Older infants

Method Country Method Country

Range between countries Method on which estimated requirements is based

3m 9m Younger infants Older infants

Method Country Method Country

Factorial method EC, NNR

Range between countries Method on which estimated requirements is based

3m 9m Younger infants Older infants

Method Country Method Country

3. Concluding remarks contexts, gender relationships, food availability, local

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