JSA COLLEGE OF AGRICULTURE AND TECHNOLOGY

Ma.Podaiyur,Thittagudi Taluk,Cuddalore Dt

Model Midsem Question Paper

Course No; and Title : AGM 301 Soil and Applied Microbiology (1+1)

Choose the best Answer:

1. Application of microbiological activities to soil fertility and crop production is which distinct
phase of Soil Microbiology?

a. Pedological Phase b. Agronomical Phase c.Ecological Phase d. Physiological phase

2. Who isolated root nodule bacterium (Rhizobium) in pure culture?

a. Martinus Beijerinck b. Louis Pasteur c. Hiltner d. Both a &b

3. Which scientist explained that Legumes took Nitrogen from air through the agency of
bacteria existing in the nodules of the roots?

a. Hillriegal b. Louis Pasteur c. Wilfrath d. Both a &c

4. Who coined the term Rhizosphere concept

a. Martinus Beijerinck b. Louis Pasteur c. Hiltner d. Both a &b

5. Who coined the term Phyllosphere ?

a. Ruinen b. Louis Pasteur c. Hiltner d. Both a &b

6. Who developed the technique of enrichment culture?

a.Martinus Beijerinck b.Sergy Winogradsky c.both a&b d.Ruinen

7. In which microorganism Winogradsky studies and establishes the concept of autotrophy?

a. Beggiatoa b. Rhizobium c.Azospirillum d.Azotobacter

8. Name the wonder drug invented by scientist Alexander Fleming?

a.Penicillin b.Streptomycin c.Neomycin d.Vancomycin

9. Who isolated the bacteria Thiobacillus thiooxidans

a.Martinus Beijerinck b.Sergy Winogradsky c.Selman A.Waksmann d.Ruinen

10. Annual addition of Ammonium Fertilizers favours which microorganism ( due to microbial

oxidation-nitric acid production)

a.Fungi b.Bacteria c.Algae d.Actinomycetes

11. Adding Nitrate to soil will inhibit which microorganism?

a.Nitrogen fixing bacteria b. fungi c.Algae d.Actinomycetes

12. Increase in Bacterial population will increases which microbe?

a.fungi b.Bacteria c.Algae d.Protozoans

13. Molecular Nitrogen constitutes what percent in the earth’s atmosphere?

a.72 % b. 73 % c. 75 % d. 78%

14. Mineralization and Immobilization are involved in which Cycle?

a. Nitrogen b. Carbon c.Sulphur d.Phosphorous

15. In which form Nitrogen will be taken up by the Paddy?

a.Nitrate b.Ammonium c.both a&b d.Ammonia

Fill Ups:
1. ----------------------- is considered as Father of Soil Microbiology.

A. Sergy Winogradsky b. Louis Pasteur c. Hiltner d. Ruinen

2. ------------ is the dominant group in soil

a.Fungi b.Bacteria c.Algae d.Actinomycetes

3. Soil region surrounding the plant root is called -----------

a.Rhizosphere b.Phyllosphere c.Phytosphere d.both a&b

4. Protozoa mainly prefer …….type of bacteria as food

a.Aerobacter b.Alkaligenes c. both a and b d.Streptomyces

5. Soil organic matter present in …………. % in peat soil

a. 40 b.45 c.35 d.70

6. The term nitrification refers to the conversion of ammonium to nitrate-------------

a.Nitrifiication b.Denitrification c. Transformation d.Assimilation

7. An actinomycete involved in nitrification is ------------- sp

a.Rhizobium b.Frankia c.Bacterai d. Azospirillum

8. Symbiotic association between fungi and root systems of higher pants is calledas…

a .Mycorrhiza b.Lichens c.Agrobacterium d.Alkaligenes

9. In starch molecule, amylopectin constitutes ……..%

a.40% b. 50% c. 60% d. 80%

10. Microbes that produce enzyme Pectinase is………

a.Bacillus b.Erwinia c.Pseudomonas d. All a , b and c

11. The breakdown of protein finally resulting in the formation of…………nitrogen

a.Ammonical b. Nitrate c.Nitrite d.Amine

12. Assimilation of Nitrogen in the form of Ammonia stored in ……..Structure of

Cyanobacteria

a. Heterocysts b.Chloroplast c.Cell Wall d.Protoplasm

13. The enzyme that hydrolyse phosphate from inositol P…….

a. Phytase b. Phosphodieaterases c. Phosphomonoesterases d.both a and b

14. Rhizobium nitrogenase is protected by ------------ present in nodules

a. Leghaemoglobin b.haemoglobin c.both a and b d.Oxygen

15. Production of chelating agents such as siderophores,an Fe chelator releases bound ------------

a.Nitrogen b.Phosphorous c.Potassium d.Oxygen

Match the following;

1. Bacteria i. Streptomyces
2. Fungi ii. Cyanobacteria
3. Actinomycetes iii. Rhizopus
4. Blue Green algae iv. Rhizobium
5. GS v. Glutamine Synthase
6. GOGAT Vi Glutamate Synthase
 7 Proteolysis Vii Protein degradation
8 Deamination Viii Hydrolysis of protein
9 Putrefication ix Ammonia to amine
10 Nitrification x Thiobacillus denitrificans
11 Nitrate reduction Xi Nitrobacter
12 Legumes Xii Alders
13 Non-legumes Xiii Green Gram
14 Free Living aerobic bacteria Xiv Clostridium
15 Free living anaerobic bacteria xv Azospirillium

Answer keys;1.iv 2.iii 3.i 4.ii 5.vi 6.v 7.viii 8.ix 9.x 10.xi 11.x 12.xiii 13.xii

14.xv. 15.xiv.

PART B:
1. In Soil the number of microorganisms is present in large number. Answer the following
questions related to it.

1. Which microorganism presents larger number in soil?

a. Fungi b. Bacteria c. Algae d. Virus

2. Which microbe present in soil will fix more nitrogen to the soil?

a.Fungi b.Bacteria c.Algae d.Amoeba

3. Which microbe present lesser in number in soil?

a.Fungi b.Bacteria c.Actinomycetes d.Protozoans

4. Which microbe will produce earthy odour to the soil during rainy season?

a.Fungi b.Bacteria c.Actinomycetes d.Amoeba

2. Actinomycetes is a microorganism which is filamentous present in soil similar to bacteria

in certain character.Answer the following questions related to it.

1. Which microbe will produce antibiotic larger in number?

a.Fungi b.Virus c.Actinomycetes d.Protozoans

2. The antibiotic Streptomycin produced from which microbe?

a.Fungi b.Virus c.Actinomycetes d.Protozoans

3. Who invented the antibiotic Streptomycin?

a.Hiltner b.Ruinen c.Selman A.Waksmann d.Sergy Winogradsky

4. Selman A.Waksmann received the nobel prize in the year?

a.1945 b.1949 c.1952 d.1956

3. Students were given two petriplates. One contain more number of microorganisms
compared to the other. Answer the following question related to it.

1. The given petriplate 1 contain more number of microrgansims due to soil

sample from ……….

a.Rhizosphere b. Non rhizosphere c. Saline soil d. Non saline soil

2. The term Rhizosphere coined by which scientist?

a.Hiltner b.Ruinen c.Selman A.Waksmann d.Sergy Winogradsky

3. The region around the root surface is called……

a.Rhizosphere b.Exosphere c.Phytosphere d.Atmosphere

4. Comparing both Rhizosphere and Non-rhizosphere in which area predominantly

microorganisms will be present?

a.Rhizosphere b.Phyllosphere c.Phytosphere d.both a and b

4. Students were observing the humus.Answer the following questions related to it.

1.The decayed organic matter is called……

a.Protein b.Carbohydrate c. both a and b d.Humus

2. Humus is rich in which content?

a. Carbon b. Nitrogen c.Helium d.Hydrogen

3. Organic matter when decomposed evolve which gas ?

a.Co2 b.N2 c.No2 d.H2

4. The source of organic matter from….

a.Plastics b.Plant source c.Animal Source d. both a and b

5. Nitrogen is the most essential nutrient for plant growth which can be fed by
microorganisms in biological form. Answer the questions relating to it..

1.The nonsymbiotic bacteria which fix nitrogen live in the soil independently are
a. Azotobacter
b. Clostridium
c. considerably less important in comparison to the symbiotic bacteria
d
all of the above
.

2.  Nitrogen fixation by the microorganisms can be detected by adopting the approach of
a. demonstrating growth in a nitrogen free medium
cultivating the microorganisms in the presence of nitrogen labeled with isotropic
b.
nitrogen
c. measuring15N2 by mass spectrometer
d
all of the above
.

3. Nitrifying bacteria can not be isolated directly by the usual techniques employed to isolate
hetrotrophic bacteria. The reasons may be due to
a
slow growth
.
b
medium growth
.
c. fast growth
d
none of these
.
4.  Bacteria, as a group, are responsible for
a. nitrogen oxidation
b. sulfur oxidation
c. nitrogen fixation
d. all of these

6. Nutrient application to the soil add fertility to it especially major nutrient N when
incorporated results good yield in legume crops. Answer the following questions related

to it.

1. The transformation of nitrates to gaseous nitrogen is accomplished by microorganisms in a

series of biochemical reactions. The process is known as
a. Nitrification
b
Denitrification
.
c. nitrogen fixation
d. Ammonification

2.  Nitrogen fixation refers to the direct conversion of atmospheric nitrogen gas into
a
Ammonia
.
b
Glucose
.
c. ATP
d
Nitrate
.

3.  The diagnostic enzyme for denitrification is

a
nitrate reductase
.
b
nitrate oxidase
.
 c. nitro oxidoreductase
d
none of these
.

4.  A heterocyst is
a. a type of spore
b
a terminally differentiated cell that fixes nitrogen
.
c. the progenitor of cyanobacterial vegetative cells
d. a cell that carries out oxygenic photosynthesis

7. Carbon is the main constituent of all cells in the world.Answer the following related
to it.

1 For rapid decomposition by microbes, the substrate should have a C/N ratio of
. a. 10-20

b. 20-30
c. 30-40
d. 60-80
2.The organic matter decomposition resulting in humus consists more amount of

a.Carbon
b.Nitrogen
c.Oxygen
d.Helium
3.Starch contains two components namely
a.amylase and amylopectin
b.amylose
c.amylopectin
d.none of these
 4. C: N ratio of humus is….
a. 10:1
b.30:1
c.20:1
d.5:1

8. Chitin is the major constituent in cell wall. Answer the questions related to it.

1. The cell wall of fungi is made up of…..

a.Murein b.Chitin c.Lignin d.both a and b

2.Example for Chitin degrading fungi

a.Saccharomyces b.Streptomyces c.Aspergillus d.Rhizobium

3.Chitin break down by the enzyme activity like

a.Chitinase b.Pectinase c.Proteinase d.both a and b

4.Chitin break down into compounds like

a.acetylglucosamine b.Hydrogen c.Oxygen d.Water

9. Students were given dark coloured organic matter for observation in powder
form.Answer the questions related to it.

1. The percentage of lignin present in Humus is.

a.45 b. 35 c.40 d.30

2.The percentage of aminoacids present in Humus is.

a.40-45 b. 30-35 c. 20-25 d.50-55

3.Fungal group that decompose Humus

a. basidiomycetes and ascomycetes

b. ascomycetes
c. basidiomycetes

d. both a and b

4.Humus fractioned into

a. Fulvic acid b. Humic acid c. Humins d. all of the these

10. The major second nutrient next to Nitrogen required for crop is
Phosphorous.Answer the questions related to it.

1.The percentage of Phosphorous in earth’s crust

a.0.1% b. 0.2% c. 0.4% d. 0.6%

2. Organic forms of P Composed of

a.lecithin b.phytin c.phospholipids d.All a, b and c

3. Inositol phosphates often called as

a.phytin b. phytic acids c.both a and b d.Nucleic acids

4. P fixation in acid soils as

a. Iron oxide b. Al oxides c. both a and b d.Calcium oxide