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Sample Problem

Given an enzyme with a KM of 0.5mM and a Vmax of 200mmol/s, the substrate concentration at which the velocity reaches 1/4 of Vmax is calculated to be 0.17mM by plugging the known values into the Michaelis-Menten equation and solving for [S].

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124 views1 page

Sample Problem

Given an enzyme with a KM of 0.5mM and a Vmax of 200mmol/s, the substrate concentration at which the velocity reaches 1/4 of Vmax is calculated to be 0.17mM by plugging the known values into the Michaelis-Menten equation and solving for [S].

Uploaded by

fintastella
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Given an enzyme with KM of 0.

5mM, at what substrate concentration will the velocity of the enzyme reach 1/4 of


the Vmax? (Vmax=200mmol/s)

Explanation:
To solve this, we need the solve for [S] in the Michaelis-Menten equation:
V max [S ]
V0 =
K M +[S ]
We know the following information:
1
KM =0.5mM; Vmax = 200mmol/s; Vo = Vmax = 50mmol/s
4
Plug in these numbers and solve for substrate concentration.

200 [S]
50 =
0.5+[S]

1 [S ]
=
4 0.5+[S]

0.5+[S] = 4([S])

0.5 = 3[S]

[S] ≈ 0.17 mM

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