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Identification of ILI / SARI / ARI Cases As Per WHO Case Definition (Referred and Systematic Collected Samples)

The document defines three types of respiratory illnesses: severe acute respiratory infection (SARI), influenza-like illness (ILI), and acute respiratory illness (ARI). It provides guidance on sample collection for real-time RT-PCR testing, including using nasal swabs or throat swabs placed in viral transport medium on ice for transport to the lab. The document outlines testing for influenza viruses and other respiratory viruses using real-time RT-PCR and duplex RT-PCR. It describes subtyping positive influenza samples to identify influenza A lineages (H1N1 or H3N2) or influenza B lineage.

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ASHOK MOHANTY
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68 views1 page

Identification of ILI / SARI / ARI Cases As Per WHO Case Definition (Referred and Systematic Collected Samples)

The document defines three types of respiratory illnesses: severe acute respiratory infection (SARI), influenza-like illness (ILI), and acute respiratory illness (ARI). It provides guidance on sample collection for real-time RT-PCR testing, including using nasal swabs or throat swabs placed in viral transport medium on ice for transport to the lab. The document outlines testing for influenza viruses and other respiratory viruses using real-time RT-PCR and duplex RT-PCR. It describes subtyping positive influenza samples to identify influenza A lineages (H1N1 or H3N2) or influenza B lineage.

Uploaded by

ASHOK MOHANTY
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Identification of ILI / SARI / ARI cases As per WHO case definition (Referred and Systematic collected samples)

Severe acute respiratory infections (SARI): An acute repiratory infection with a history of fever or measured fever of ≥ 38° C and cough with onset within the last 7
days and requires overnight hospitalization.
Influenza like Illness (ILI): Acute onset within the last 10 days following respiratory symptoms, measured fever of ≥ 38° C and cough.
Acute respiratory illness (ARI): Sudden onset of respiratory infection symptoms ( cough, sore throat shortness of breath, coryza)

Sample Collection: Optimum viability of Influenza virus is at 2-8oC; NP swab / Throat swab / Nasal aspirate (place on ice / cold packs at 2-80C /
40C for transport to lab) in vial containing 1-3 ml VTM. Swab should be collected with Synthetic tip (Polyster / Plastic shift)

Real time RT-PCR (test as soon as collected/ Referred)

Testing for other Respiratory


viruses
+ve Influenza virus

Typing by Real time RT-PCR Duplex RT-PCR

Respiratory Syncytial
+ve Influenza A virus +ve Influenza B virus Viruses RSV A & B
Real time PCR for lineages
Sub typing by Real time RT-PCR Para Influenza Viruses 1 to 4
Human metapneumo Viruses
Yamagata Victoria
A H1N1 virus A H3N2 virus Rhino virus
Adeno Virus
Allelic disremination rt RT-PCR for A(H1N1)pdm09

Influenza positive Isolation in MDCK Cell


Lines

Genetic Characterization Antigenic characterization


Sequencing of HA / NA
Phenotypic Assay
Antiviral Susceptibility

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