Floating Disk Lab 2018

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Name _____________________________________Date

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FLOATING DISK LAB: DAY 1 - INVESTIGATION


Objective:
To determine the net rate of photosynthesis by calculating the speed of oxygen production.

Fact: ​Plants make more sugars in 24 hours than they need, up to 160 billion metric tons per
year! Thus, plants are a major global food source and basis of the food web.

Pre-Lab: Class Discussion


Using your basic knowledge of plants and photosynthesis, what are various VARIABLES that
could affect or alter the actual process of photosynthesis and sugar production?

Environmental Factors: Physical Plant Factors: Other factors:

Background Information:
In the floating leaf disk lab, you will be using syringes to create a vacuum that will remove to
trapped air with the leaf cells allowing the leaves to fall to the bottom of the beaker. By
extracting the oxygen from the leaves, one can now calculate the time it takes for a plant to
produce oxygen and sugar as a by product of photosynthesis and thus establish a rate of
reaction or speed of which it happens. Once the leaves start producing oxygen within its cells,
the oxygen gets trapped first in the mesophyll, before its released to the environment; allowing
the plant cells to become buoyant and float to the top of the beaker.

To stimulate photosynthesis the following supplies will be used:

Lab Item: What it represents:

LAMP SUNLIGHT

BAKING SODA CARBON DIOXIDE (CO2)

WATER WATER (H2O)

FLOATING DISK OXYGEN (O2) Produced


Hypothesis/ Claim:

If
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Then
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Data Table:

ENVIRONMENT: INTENSE LIGHT (5 cm from the light)


Time 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
(minutes)

# of Disks

ENVIRONMENT: REGULAR LIGHT (10 cm from the light)


Time 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
(minutes)

# of Disks

ENVIRONMENT: LOW LIGHT (20 cm from the light)


Time 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
(minutes)

# of Disks

What is your INDEPENDENT VARIABLE in this study? _____________________________


What is your DEPENDENT VARIABLE in this study? _______________________________
What is your CONSTANT VARIABLES in this study? _______________________________

Graph Your results:


FLOATING DISK LAB: DAY 2 - MODELING

Part 1 - INITIAL MODEL:

As a group, use the giant ​WHITE BOARDS ​to illustrate what is happening in your
experiment and include aspects and factors on the MOLECULAR LEVEL. Thus, incorporate
CO2, H2O, C6H12O6, Light, O2, pigments, chloroplast, what the molecules look like, how and
what they changed into, etc.
LABEL everything and be specific! You will have 25 minutes.

Part 2 - STICKY NOTE REVISION:

You now have 10 minutes to grab 3 sticky notes and venture to 3 different white board
models around the room. Place a sticky note SUGGESTION on their boards with helpful
information that they may be missing or how to make the models better.
When you are finished, go back to your white board model and read all the sticky notes.
Take another 10 minutes to REVISE your model based on the class suggestions.

Part 3: FINAL MODEL:

Using the space provided DRAW out your groups final model. Make sure to label
everything.
Analysis:
1) What is the formula for photosynthesis?

2) What products are produced from photosynthesis?

3) What is the purpose of the baking soda in this experiment?

4) Determine the mean rate of photosynthesis in each condition of your study (5cm, 10cm,
20 cm). To calculate the rate of photosynthesis, use the formula below & SHOW your
work:
​(final # of disks floating)​ = _________ disks/ sec.
Total time (seconds)
RATE OF PHOTOSYNTHESIS:
Distance: 5 cm Distance: 10 cm Distance: 20 cm

5) If you increase the light intensity during your experiment, the rate of photosynthesis
would decline. Explain why that might happen.

6) During your study, you should have seen a steady increase of floating disks over time
when the light was turned on. However, when the disks were not given light they started
to sink. Why?
7) Some plants like desert cactus can store enormous amounts of water within their cells.
However, they do NOT photosynthesize during the day because the intense heat would
evaporate their water too fast, halting photosynthesis and energy production. Thus
cactus photosynthesize at night. Propose an explanation on HOW they would do this?

8) If you decrease or increase environmental temperatures around a plant the


photosynthesis process stops, thus creating a bell shaped curve graph. Explain how
temperature can affect the rate of photosynthesis in a plant and DRAW a graph to
illustrate your point.
9) Using the following graph, analyze what is happening between the oxygen and carbon
dioxide and EXPLAIN WHY. Then, determine a situation where this would actually exist in.

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