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Determination of Non-Ionic Surfactants Using HPLC: Method

This document describes a method for determining non-ionic surfactants using HPLC. It uses a C18 column with a mobile phase of 90:10 methanol/water at a flow rate of 1.5 ml/min. Detection is via refractive index. The method can separate 12 different non-ionic surfactants including polyethylene glycols, polyoxyethylene sorbitane monolaurate, and lauryl alcohol polyglycol ether.

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63 views1 page

Determination of Non-Ionic Surfactants Using HPLC: Method

This document describes a method for determining non-ionic surfactants using HPLC. It uses a C18 column with a mobile phase of 90:10 methanol/water at a flow rate of 1.5 ml/min. Detection is via refractive index. The method can separate 12 different non-ionic surfactants including polyethylene glycols, polyoxyethylene sorbitane monolaurate, and lauryl alcohol polyglycol ether.

Uploaded by

str_ila
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Determination of non-ionic Surfactants using HPLC

Method VEV0028J
HPLC RP Mode

Column: Eurospher 100-5 C18, 250 x 4.0 mm ID Order No. 25DE181ESJ

Phase: Eurospher 100-5 C18

Conditions: Eluent: MeOH / Water (90:10)


Gradient: isocratic
Flow rate: 1.5 ml/min
Temperature: 25 °C
Volume: 20 µl

Detection: Refractive Index (RI)

Substances: Polyethylene glycol, Polyoxyethylene sorbitane monolaurate, Nonylphenol


polyglycol ether, Laurylalcohol, Laurylalcohol polyglycol ether, Oleic acid
diethanolamide, Oleic amide polyglycol ether, Myristylalcohol, Myristyl alcohol
polyglycol ether, Palmitic acid polyglycol ester, Polyoxyethylene glycerol
monostearate, Stearic acid polyglycol ester

Keywords: Surfactants

Chromatogram: 1
1. Polyethylene glycols
2. Polyoxyethylene sorbitane monolaurate
3. Nonylphenol polyglycol ether
4. Lauryl alcohol 3
5. Lauryl alcohol polyglycol ether 6
6. Oleic acid diethanolamide
7. Oleic amide polyglycol ether 5
8. Myristyl alcohol
9. Myristyl alcohol polyglycol ether 8
10. Palmitic acid polyglycol ester 4 7
11. Polyoxyethylene glycerol monostearate
12. Stearic acid polyglycol ester. 2 10
12
9 11

0 5 10 15 min

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