Sop Mouse Injection Subcutaneous

SOP: Mouse Subcutaneous Injections
These SOPs were developed by the Office of the University Veterinarian and reviewed by Virginia Tech
IACUC to provide a reference and guidance to investigators during protocol preparation and IACUC reviewers
during protocol review. They can be used as referenced descriptions for procedures on IACUC protocols.
However, it is the sole responsibility of the Principal Investigator to ensure that the referenced SOPs adequately
cover and accurately represent procedures to be undertaken in any research project. Any modification to
procedure as described in the SOP must be outlined in each IACUC protocol application (e.g. if the Principal
Investigator plans to use a needle size that is not referenced in the SOP, simply state that alteration in the
IACUC protocol itself).
Table of Contents
I. Procedure Summary & Goal ......................................................................................... 1

II. Personal Protective Equipment & Hygiene ................................................................... 1
III. Supply List..................................................................................................................... 1
IV. Detailed Procedure ........................................................................................................ 1
V. Variations ...................................................................................................................... 2
VI. Potential Adverse Effects, Mitigation, or Treatment..................................................... 3
VII. References ..................................................................................................................... 3
Version: 1
Original Date: 12/12/17
Version date: 12/12/17
SOP: MOUSE SUBCUTANEOUS INJECTIONS
Describes procedure for the administration of substances via subcutaneous (the space between the skin and
underlying muscle) injections.
Considerations
1. Useful for large volumes of fluids. Warm fluids to body temperature prior to use.
2. Several sites can be used for multiple injections – dorsal surface, lateral thoracic region, lower
quadrants of abdomen.
3. Can be performed with on-handed restraint technique.
4. Please refer to the Guidelines for Injections in Rodents and Rabbits, Virginia Tech Office of the
University Veterinarian for recommended volumes and needles sizes.
I. Personal Protective Equipment (PPE) and Hygiene

a. Ensure appropriate PPE is used to protect technician from accidental exposure to blood and other body
fluids, such as:
i. Gloves
ii. Eye protection
iii. Mask
iv. Other PPE as required by protocol/facility
b. Hands should be washed and/or gloves changed between animals.
c. Promptly dispose of used sharps in the provided leak-proof, puncture resistant sharps container.
II. Supply List

a. Needles (25 - 27 gauge; ⅝– 1 inch)
b. Prefilled syringes; warm fluids to body temperature prior to use.
c. Gauze pads
III. Detailed Procedure

a. Frequency
i. Fluids can be administered as often as required, utilizing different locations, depending upon
absorption rate of fluids.
b. Anesthesia
i. No anesthesia required.
c. Procedure
1
i. Manually restrain the animal using the scruff method.

ii. Pull a tent of skin taut to separate the epidermal/dermal layers from the musculature of the
animal, creating a subcutaneous space.
iii. With the needle pointing away from you, insert at a 45° angle in the subcutaneous space, being
careful to not push through the other side (Figure 1).
Figure 1. Insert Needle into Subcutaneous Pocket (Tenting)
iv. Aspirate the syringe prior to administration. If blood or any other material is drawn back into
the chamber, then withdraw needle and repeat placement.
v. Inject into the site; a small bleb (formation of fluid ppocket) in the subcutaneous space should
be noted (Figure 2).
Figure 2. Note Small Fluid Pocket (Bleb)
vi. Remove needle when complete, and apply small amount of pressure to the site to prevent
backflow of the material.
vii. Dispose of the needle into approved sharps container.
IV. Variations
None
2
V. Potential Adverse Effects, Mitigation, or Treatment

a. Hematoma/bruising at injection site
b. Pain or redness at injection site
c. Abscess
i. Contact veterinary personnel for treatment options
d. Induration at injection site
e. Hypersensitivity to injected substance
i. If you notice a rash or hives, fever, difficulty breathing, vomiting, facial swelling, contact
veterinary personnel immediately
VI. References
American Association of Laboratory Animal Science. Laboratory Animal Technician Training Manual.
(Memphis, TN: Drumwright and Co, 2007)
Charles River SOP 2405-3 - Dosing of Rodents – TGS and Discovery Services
Charles River Insourcing Solutions. Biomethodology of the Laboratory Mouse
Hawk, C.T., Leary, S.T., and Morris, T.H. Formulary for Laboratory Animals (3rd ed.). (Ames, Iowa:
Blackwell Publishing, 2005)
http://www.research.usf.edu/cm/docs/Formulary_for_Lab_Animals_3rd_ed.pdf
National Institutes of Health Animal Research Advisory Committee. Guidelines for Survival Bleeding in
Mice & Rats. http://oacu.od.nih.gov/ARAC/survival.pdf
Suckow, M., Danneman, P., and Brayton, C. The Laboratory Mouse. (Boca Raton, FL: CRC Press LLC,
2001)
Turner, P.V., Brabb, T., Pekow, C., and Vasbinder, M. Administration of Substances to Laboratory
Animals: Routes of Administration and Factors to Consider. J Am Assoc Lab Anim Sci.; 50(5): 600–613.
(2011 September) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189662/

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SOP: Mouse Subcutaneous Injections

I. Procedure Summary & Goal ......................................................................................... 1

I. Personal Protective Equipment (PPE) and Hygiene

II. Supply List

III. Detailed Procedure

i. Manually restrain the animal using the scruff method.

Figure 1. Insert Needle into Subcutaneous Pocket (Tenting)

Figure 2. Note Small Fluid Pocket (Bleb)

V. Potential Adverse Effects, Mitigation, or Treatment

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