Physioex Lab Report: Pre-Lab Quiz Results

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This experiment measured glucose levels in patient samples and used a glucose standard curve to determine concentrations. It also provided information about diabetes diagnoses.

The experiment showed different glucose levels in samples and allowed identification of normal vs diabetic/pre-diabetic ranges. It also linked high levels to diabetes diagnoses.

Patient 1 had a normal reading, while patients 3 and 5 were in the diabetic range. Patient 2 was borderline impaired.

1/22/2021 PhysioEx Exercise 4 Activity 2

PhysioEx Lab Report


Exercise 4: Endocrine System Physiology
Activity 2: Plasma Glucose, Insulin, and Diabetes Mellitus
Name: beija
Date: 22 January 2021
Session ID: session-63448cfb-a79e-f068-ecf1-d93a917cdc09

Pre-lab Quiz Results


You scored 80% by answering 4 out of 5 questions correctly.

1 Which of the following statements is false?

You correctly answered: Insulin is a hormone secreted into the stomach to aid with starch
digestion.

2 Which of the following statements is true?

You correctly answered: All of these statements are true.

3 A diagnosis of type 1 diabetes mellitus implies that

You correctly answered: the pancreas is not producing sufficient insulin.

4 A diagnosis of type 2 diabetes mellitus implies that

You correctly answered: the cells of the body are unresponsive to circulating insulin.

5 Glucagon is a hormone

Your answer: that promotes the formation of glycogen.

Correct answer: that opposes the action of insulin.

Experiment Results
Predict Question
1 Predict Question: To measure the amount of plasma glucose in a patient sample

Your answer: the glucose concentration of the sample will be measured and the optical
density will be extrapolated from the glucose standard curve.

Stop & Think Question


1 Why does the color in the tubes vary?
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1/22/2021 PhysioEx Exercise 4 Activity 2

You correctly answered: The tubes contain different amounts of glucose.

Experiment Data
Tube Optical Density Glucose
(mg/deciliter)
Part 1 1 0.30 30
2 0.50 60
3 0.60 90
4 0.80 120
5 1.00 150
Part 2 1 0.73 104
2 0.79 115
3 0.89 133
4 0.83 122
5 0.96 144

Post-lab Quiz Results


You scored 100% by answering 6 out of 6 questions correctly.

1 A male patient has had successive fasting plasma glucose readings of 115, 110, and 122
mg/dl. The healthcare provider will inform him that

You correctly answered: he appears to have impairment or borderline impairment of insulin-


mediated glucose uptake by his cells.

2 To obtain an accurate spectrophotometric measurement of the glucose concentration in the


sample

You correctly answered: heparin is added to prevent blood clots.

3 In the spectrophotometric assay used in this experiment, the __________ as the glucose
concentration in the sample increases.

You correctly answered: optical density increases.

4 A female patient has had successive fasting plasma glucose readings of 130, 140, and 128
mg/dl. The healthcare provider will inform her that

You correctly answered: she has developed diabetes.

5 To maintain plasma glucose homeostasis

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1/22/2021 PhysioEx Exercise 4 Activity 2

You correctly answered: insulin-mediated transport of glucose into cells acts as negative
feedback when plasma glucose levels rise.

6 A laboratory technician withdraws a blood sample from a vein in your upper arm knowing
that

You correctly answered: the plasma glucose concentration will be the same in both the arm
vein and the index finger.

Review Sheet Results


1 What is a glucose standard curve, and why did you need to obtain one for this experiment?
Did you correctly predict how you would measure the amount of plasma glucose in a patient
sample using the glucose standard curve?

Your answer:

The glucose standard curve is a graphic tool that illustrates the relationship between optical
density and glucose concentration. We utlized this tool because it was the backdrop for
comparison for the patient samples. I did correctly predict how I would measure the
amount of plasma glucose in a patient sample using the glucose standard curve because the
optical density is used to help find out how much glucose was in the sample.

2 Which patient(s) had glucose reading(s) in the diabetic range? Can you say with certainty
whether each of these patients has type 1 or type 2 diabetes? Why or why not?

Your answer:

Patient 3 and patient 5 had glucose readings that were considered to be in the diabetic
range (above 126mg/dl). I cannot say with certainty whether each of these patients has type
1 or type 2 diabetes because you would need more data such as the amount of insulin
hormone and the response that the person's body has to the insulin. From this data, we only
know that the patients have high blood glucose levels and have diabetes.

3 Describe the diagnosis for patient 3, who was also pregnant at the time of this assay.

Your answer:

This would likely be gestational diabetes. This could be because the placenta makes
hormones that cause glucose to build up in the blood.

4 Which patient(s) had normal glucose reading(s)?

Your answer:

Patient 1 had normal glucose ratings (under 110 mg/dl) with a reading of 104 mg/dl.
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1/22/2021 PhysioEx Exercise 4 Activity 2

5 What are some lifestyle choices these patients with normal plasma glucose readings might
recommend to the borderline impaired patients?

Your answer:

Consuming less sugar and starchy foods, consuming more fiber and high-quality fat and
protein. Increasing consumption of low-starch vegetables and time-restricted eating.

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