MSDSPL04_rev0_220211

MATERIAL SAFETY DATA SHEET

VIVANTIS TECHNOLOGIES SDN BHD

REVONGEN CORPORATION CENTER
12A, Jalan TP5, Taman Perindustrian UEP,
47600 Subang Jaya, Selangor, Malaysia.

For Information:
Tel: +603-8025 1603 Email: info@vivantechnologies.com
Fax: +603-80351637 / 1354 URL: www.vivantechnologies.com

Date prepared: 3rd January 2017 Reviewed: 9th January 2017

SECTION 1: CHEMICAL IDENTIFICATION

Code: GF-BA-050; GF-BA-100; GF-BD-050; GF-BD-100; GF-BT-050; GF-BT-100;
GF-FD-025; GF-FE-025; GF-GC-050; GF-GC-100; GF-GC-200; GF-GP-050;
GF-GP-100; GF-GP-200; GF-MT-025; GF-MT-050; GF-MT-100; GF-PC-050;
GF-PC-100; GF-PC-200; GF-PL-050; GF-PL-100; GF-PL-200; GF-PT-050;
GF-PT-100; GF-RD-025; GF-RD-050; GF-RD-100; GF-SD-025; GF-TB-025;
GF-TD-050; GF-TD-100; GF-TR-025; GF-TR-050; GF-TR-100

Name: GF-1 Bacterial DNA Extraction Kit; GF-1 Blood DNA Extraction Kit;
GF-1 Tissue Blood Combi DNA Extraction Kit; GF-1 Forensic DNA Extraction Kit;
GF-1 Food DNA Extraction Kit; GF-1 AmbiClean Kit (Gel & PCR);
GF-1 Gel DNA Recovery Kit; GF-1 microTotal RNA Extraction Kit;
GF-1 PCR Clean-Up Kit; GF-1 Plasmid DNA Extraction Kit;
GF-1 Plant DNA Extraction Kit; GF-1 Viral Nucleic Acid Extraction Kit;
GF-1 Soil Sample DNA Extraction Kit; GF-1 Blood Total RNA Extraction Kit;
GF-1 Tissue DNA Extraction Kit; GF-1 Total RNA Extraction Kit

SECTION 2: COMPOSITION/INFORMATION ON INGREDIENTS

Chemicals in buffer solution. Volume of each product package varies.

Chemical Name: CAS#: %

Acetic Acid 64-19-7 <5
Calcium Chloride, anhydrous 7440-70-2 <1
DNase I 9003-98-9 <5
EDTA 6381-92-6 <10
Glycerol 56-81-5 <1
Guanidine Hydrochloride 50-01-1 <50
 MSDSPL04_rev0_220211

Guanidine Thiocyanate 593-84-0 <60

Magnesium Chloride 7791-18-6 <1
Nuclease, deoxyribo- 9003-98-9 <90
Phenol Red 143-74-8 <1
Potassium Acetate 127-08-2 <1
Proteinase K 39450-01-6 <5
Ribonuclease A 9001-99-4 <5
Sodium Acetate 127-09-3 <2
Sodium Chloride 7647-14-5 ≤2.33
Sodium Dodecyl Sulfate 151-21-3 <8
Tris Hydrochloride 1185-53-1 ≤7.88
Triton X-100 9002-93-1 <50
Tween 20 9005-64-5 <1

SECTION 3: HAZARDS IDENTIFICATION

The product is a kit consisting of different buffers, which different buffers are produced using
individual ingredients. The classification of the ingredients can be obtained from section 3.

GHS Classification

Signal word: Harmful (slight)

Hazard statements:
H302 + H332: Harmful if swallowed or if inhaled
H315: Causes skin irritation
H317: May cause an allergic skin reaction
H318: May cause serious eye damage
H334: May cause allergy or asthma symptoms or breathing difficulties if inhaled

Precautionary statements:
P261:
P305+P351+P338+P310: Rinse cautiously with water for at least 15 minutes if contect with eyes.
Remove contact lenses, if present and easy to do and continue rinsing.
Call a poison center or doctor or physician immediately.
P342+P311: Call a poison center or doctor or physician if experiencing respiratory
symptoms.
P362+P364: Take off contaminated clothing and wash it before use.
 MSDSPL04_rev0_220211

SECTION 4: FIRST-AID MEASURES

In case of inhalation, remove to fresh air. If unconscious, place in recovery position. Seek medical
assistance if there are problems.
In case of skin contact, wash with soups and excess water for at least 15 minutes. Seek medical
assistance if irritation is persistent.
In case of eye contact, wash copiously with water for up to 15 minutes. Small amounts splashed into
eyes can cause tissue damage and blindness. Remove contact lenses and continue rinsing. Seek
medical assistance if there is persistent irritation.
In case of ingestion, wash out mouth with water and drink afterwards plenty of water provided the
person is conscious. Do not induce vomiting. Do not give milk or alcoholic beverages. Never give
anything by mouth to an unconscious person. Seek medical assistance if there is abdominal
discomfort.

SECTION 5: FIRE FIGHTING MEASURES

Use extinguishing measures that are appropriate to local circumstances and the surrounding
environment.
Suitable extinguishing media: Alcohol resistant foam, carbon dioxide, dry chemical, or water
spray or water spray
Hazardous combustion products: Carbon dioxide, carbon monoxide, dense smoke
Upper flammable limit: Not available
Lower flammable limit: Not available
Water or foam may cause frothing if liquid is burning but it still may be a useful extinguishing agent
if carefully applied to the fire. Do not direct a water stream directly into the hot burning liquid. Use
water spray/fog for cooling.
During fire-fighting, do not enter fire area without proper protection including self-contained
breathing apparatus and full protective equipment. Fight fire from a safe distance and a protected
location due to the potential of hazardous vapors and decomposition products.
Wear self-contained breathing apparatus for firefighting if necessary.
Collect contaminated fire extinguishing water separately. This must not be discharged into drains.
Fire residues and contaminated fire extinguishing water must be disposed of in accordance with local
regulations.

SECTION 6: ACCIDENTAL RELEASE MEASURES

For personal protection, use personal protective equipment and clothing as needed. Ensure adequate
ventilation.
For environmental precautions, prevent product from entering drains. Prevent further leakage or
spillage if safe to do so. Exposure to the spilled material may be irritating or harmful. Additional
precautions may be necessary based on special circumstances created by the spill including: the
material spilled, the quantity of the spill, the area in which the spill occurred.
For cleaning up, soak up with inert absorbent material (e.g. sand, silica gel, acid binder, universal
binder, sawdust). Keep in suitable and closed containers for disposal.
 MSDSPL04_rev0_220211

SECTION 7: HANDLING AND STORAGE

Keep containers tightly closed in a dry, cool and well-ventilated place to protect product quality.
Handle in accordance with good industrial hygiene and safety practice. Avoid creating dust and
tearing bags.
Wash thoroughly after handling; remove contaminated clothing and wash before re-use.
For safe handling, avoid contact with skin and eyes. Avoid exposure by obtaining special instructions
before use. Avoid smoking, eating and drinking in application area. Provide sufficient air exchange
and/or exhaust in work rooms. To prevent leaks or spillages from spreading, provide a suitable liquid
retention system.

SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION

This product does not contain any hazardous materials with occupational exposure limits established
by the region specific regulatory bodies.
Eye protection
An eye wash station must be available where this product is used. Wear chemical goggles.
Skin protection
Avoid skin contact by wearing chemically resistant gloves, a lab coat and other protective equipment
depending upon conditions of use. Inspect gloves for chemical break-through and replace at regular
intervals. Clean protective equipment regularly. Wash hands and other exposure areas with mild soap
and water before eating, drinking, and when leaving work. Have a safety shower available.
Respiratory protection
A respiratory protection program that meets OSHA’s 29 CFR 1910.134 and ANSI Z88.2
requirements must be followed whenever workplace conditions warrant a respirator’s use.

SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES

Different kits have different buffers, the chemical properties are different for different buffers.
Appearance: Liquid
White lyophilized form – Proteinase K and carrier RNA
Color: Colorless
Odor: Odorless
pH: different pH for different buffers
Melting Point: No data available
Boiling Point: No data available
RNase-free Water: 100°C
Flash Point: No data available
Ignition Point: No data available
Flammability: The product is not flammable and does not sustain combustion
Explosive Properties: No data available
Oxidizing Properties: substance is not classified as oxidizing
Vapour Pressure: No data available
RNase-free Water: 23 hPa (20°C)
Relative Density: No data available
 MSDSPL04_rev0_220211

Water Solubility: completely miscible

Viscosity: No data available

SECTION 10: STABILITY AND REACTIVITY

Stability: Stable under normal conditions
Conditions to be avoided: Heat, flames and sparks
Materials to avoid: Strong acids and oxidizing agents, bases
Hazardous decomposition products: None known
Hazardous polymerization: Hazardous polymerization will not occur

SECTION 11: TOXICOLOGICAL INFORMATION

Acute toxicity: Harmful if swallowed
Acute oral toxicity:
EDTA: LD50 oral – rat – 2000mg/kg
LD50 oral – rabbit – 2300mg/kg
Guanidine hydrochloride: LD50 oral – rat – 475mg/kg
LD50 oral – mouse – 571mg/kg
Triton X-100: LD50 oral – rat – 1900 – 5000 mg/kg
Sodium dodecyl sulfate: LD50 oral – rat - 1288mg/kg
Sodium hydroxide: LD50 oral – rabbit – 500mg/kg
Acute inhalation toxicity:
Guanidine hydrochloride: LC50 – female rat – 3.2mg/l, 4hrs
LC50 – male and female rat – 5.4mg/l, 4hrs
LC50 – male rat – 7.7mg/l, 4hrs
Acute dermal toxicity:
Guanidine hydrochloride: LD50 dermal – rabbit - >2001 mg/kg
Triton X-100: LD50 dermal – rabbit - >3000 mg/kg
Sodium dodecyl sulfate: LD50 dermal - >10g/kg
Primary irritant effect:
On the skin and eye: no irritating effect
Sensitization: no sensitizing effects known
Carcinogenicity: No classified based on available information
Germ cell mutagenicity: No classified based on available information
Reproductive toxicity: No classified based on available information
Aspiration toxicity: No classified based on available information
Specific target organ toxicity – single exposure: No classified based on available information
Specific target organ toxicity – repeated exposure: No classified based on available information
Other Toxicological Information
Contains a substance(s) that is a possible reproductive system hazard based on high dose tests with
laboratory animals.
 MSDSPL04_rev0_220211

SECTION 12: ECOLOGICAL INFORMATION

Do not allow undiluted product or large quantities of it to reach ground water, water course or
sewage system.
No ecological information available.

SECTION 13: DISPOSAL CONSIDERATIONS

Observe all federal, state and local environmental regulations.
Do not contaminate ponds, waterways or ditches with chemical or used container, send to a licensed
waste management company.
Empty containers should be taken to an approved waste handling site for recycling or disposal. Do
not re-use empty containers.

SECTION 14: TRANSPORT INFORMATION

UN no: Not regulated as a dangerous good
Not regulated as a dangerous good in the meaning of ADR/RID, ADNR, IMDG-Code, ICAO/IATA-
DGR.

SECTION 15: REGULATORY INFORMATION

R22: Harmful if swallowed
R36/38: Irritating to eyes and skin
R41: Risk of serious damage to eyes
R42: May cause sensitisation by inhalation
R43: May cause sensitization by skin contact

S26: Rinse eyes immediately with plenty of water

S36/37/39: Wear suitable protective clothing, gloves and eye/face protection

SECTION 16: OTHER INFORMATION

The information contained in this MSDS relates only to the material(s) designed and does not relate
to use(s) in combination with any other material, process(es) and /or chemical reaction(s). Vivantis
Technologies Sdn. Bhd. provides this information in good faith, from sources believed to be
accurate; however, Vivantis assumes no liability for its accuracy or completeness, and thus shall not
be held liable for any damage resulting from handling or from contact with the above product.

All Vivantis products are supplied for manufacturing, research and laboratory use only. Researchers
and laboratory personnel intending to use any of these products for medical investigation on human
are solely responsible for such use and for compliance with the pertinent regulations of the United
States Food & Drug Administration (US-FDA) and other regulations. We do not assume liability for
damages resulting from the handling, use and/or disposal of these products, from their use in
violation of patent or other rights or reliance upon this information.