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Blood Typing Technique

The document describes the process of blood typing by using a needle to withdraw blood, placing drops of the blood and anti-A and anti-B serum on a labeled slide, and observing if agglutination occurs to determine blood type. Blood type A results from agglutination with anti-A serum only, type B from anti-B only, type AB from both, and type O if there is no agglutination. Anti-D is also tested to determine if the blood is Rh positive or negative. The process is demonstrated on 4 samples, identifying their blood types as A positive, B positive, AB positive, and O negative.

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Sara Emad
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57 views2 pages

Blood Typing Technique

The document describes the process of blood typing by using a needle to withdraw blood, placing drops of the blood and anti-A and anti-B serum on a labeled slide, and observing if agglutination occurs to determine blood type. Blood type A results from agglutination with anti-A serum only, type B from anti-B only, type AB from both, and type O if there is no agglutination. Anti-D is also tested to determine if the blood is Rh positive or negative. The process is demonstrated on 4 samples, identifying their blood types as A positive, B positive, AB positive, and O negative.

Uploaded by

Sara Emad
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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BLOOD TYPING TECHNIQUE Name: Yomna Adel

1. Under antiseptic techniques, a needle is used to withdraw some blood from a vein ex: arm.
2. Get a sterile Slide, and label one end as Anti A, and the other as Anti B. Add a drop on Anti-A
on the Anti-A labelled side, and a drop of Anti-B on the other.

3. Add one drop of blood to each end and mix well.

4. The subject is blood Group A if agglutination occurred with the Anti-A serum;
Group B if agglutination occurred with the Anti-B serum, group AB if agglutination occurred
with both test serums, and O if there was no agglutination.

5. Anti-D can also be added and thus can be Rh positive or Rh negative.

Part 2
 For the First blood sample, we added, Anti-A, Anti-B and Anti-D, but there was
agglutination only with Anti-A and Anti D, which means this is blood group A and is also
Rh positive.
 For the Second blood sample we also added the same, there was agglutination with
Anti-B and with Anti-B, so blood group B and Rh positive.
 For the third we added the same, there was agglutination with all so blood group AB
and Rh positive.
 For the last one, there was no agglutination at all so Blood Group O and Rh negative.

Thank you.

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