INSTRUCTION MANUAL

892-9263-006 Ver.7 June. 2017

OPTION RACK
FOR CHROMASTER
5210/5260
AUTOSAMPLER
 This product is intended for research use only. It is not to be used for
reporting patient diagnostic or therapeutic results.

 Before using the instrument, read the safety

instructions and precautions carefully.
 Be sure to observe the safety instructions in this
manual and the WARNING/CAUTION labels on the
instrument.
 Keep this manual in a safe place nearby so it can
be referred to whenever needed.

NOTICE:

1. The Information contained in this document is subject to change

without notice for improvement.
2. This manual is copyrighted by Hitachi High-Tech Science
Corporation with all rights reserved.
No part of this manual may be reproduced, transmitted or
disclosed to a third party in any form or by any means without the
express written permission of Hitachi High-Tech Science
Corporation.
3. Hitachi High-Tech Science Corporation assumes no liability for any
direct, indirect, or consequential damages arising from use not
described in this manual.
Utmost care must be exercised when using the instrument.
4. This document does not provide any warranty or permission for
industrial properties or any rights to grant license lawfully and
without infringement.

24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo, Japan

Copyright © Hitachi High-Tech Science Corporation 2013.

 PREFACE

We thank you for purchasing the option rack for the Chromaster 5210/5260 autosampler.
This option rack is used for combining the specified sample vials with the Chromaster
5210/5260 autosampler. Samples showing physiological activity cannot be used with this
instrument since there is a possibility of bacterial or viral infection.

This instrument is intended for use by persons having a basic knowledge of chemical
analysis procedures. Keep in mind that improper use of analytical instruments,
chemicals or samples would result not only in wrong analytical data but also in
consequences adverse to safety. Note that it is allowed only for persons having a
basic knowledge of chemical analysis procedures to use this instrument.

Please read this instruction manual carefully before attempting operation and acquaint
yourself with the option rack to enable its correct use.

ABOUT THIS MANUAL

● The operating procedures, maintenance & checkup instructions and precautions for the
option rack for Chromaster 5210/5260 autosampler are described in this manual.

● This manual consists of the following sections.

IMPORTANT (contains details of warranty, installation/relocation, after-sale technical

service, useful life of this instrument, etc.)
SAFETY SUMMARY
Chapter 1. OVERVIEW (Sample Racks for Standard Autosampler, etc.)
Chapter 2. FUNCTIONS (Explanation of Each Control Knob and Switch, etc.)
Chapter 3. OPERATION (Preparatory Operations, etc.)
Appendix 1. INSTALLATION (Assembling, etc.)

● First of all, read IMPORTANT and SAFETY SUMMARY at the beginning of the instruction
manual for Chromaster 5210/5260 autosampler and those at the beginning of the
instruction manual for the organizer. Then read IMPORTANT and SAFETY SUMMARY
at the beginning of this instruction manual.
If installation of the option rack is not yet completed, refer to the Appendix.

-1-
 IMPORTANT
Warranty on Product

Limited Warranty

The option rack for Chromaster 5210/5260 autosampler is warranted to be free from defects in
material or workmanship under normal use within the product specifications indicated in this
manual and under conditions given below. This warranty is void if the instrument is not used
according to the instruction manual.
The manufacturer makes no warranties, either express or implied, except as provided herein,
including without limitation thereof, warranties as to marketability, merchantability, for a
particular purpose or use, or against infringement of any patent.
No oral or written information or advice given by the manufacturer, its dealers, distributors,
agents or employees shall create a warranty or in any way increase the scope of this warranty.

Scope of Warranty

Any parts which prove to be defective in design or workmanship during the warranty period will
be repaired, adjusted or replaced without charge. A substitute part may be used for repair, or
replacement with an equivalent product may be made instead of repair. Such system
components as a personal computer and printer to be updated frequently for improvement may
not be available in original versions at the time of replacement.
Note that this warranty does not apply to the instrument after it is discarded, or if modified by
the user or resold without permission from the manufacturer, consumable parts, and any failure
of lifetime-expired parts.
The manufacturer assumes no liability for any damage to data or application software due to
any possible fault or failure of this instrument.

Warranty Period

One year from the date of initial installation.

(In case a separate warranty document has been issued, the warranty period indicated in it
takes precedence over the above period.)

IMPORTANT - 1
Warranty Limitations and Exclusions

Disclaimer of Warranty

THE MANUFACTURER MAKES NO WARRANTIES, EITHER EXPRESS OR IMPLIED,

EXCEPT AS PROVIDED HEREIN, INCLUDING WITHOUT LIMITATION THEREOF,
WARRANTIES AS TO MARKETABILITY, MERCHANTABILITY, FOR A PARTICULAR
PURPOSE OR USE, OR AGAINST INFRINGEMENT OF ANY PATENT. IN NO EVENT SHALL
THE MANUFACTURER BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY
DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE GIVEN BY THE MANUFACTURER, ITS
DEALERS, DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL CREATE A WARRANTY OR
IN ANY WAY INCREASE THE SCOPE OF THIS WARRANTY.

Note that the following cases are excluded from the scope of this warranty, i.e., these cases are
beyond the coverage of free-of-charge repair even during the warranty period indicated above.

(a) Failure due to operation at a place not meeting the installation requirements specified by
the manufacturer.

(b) Failure due to power supply voltage/frequency other than specified by the manufacturer or
due to abnormality in power supply.

(c) Corrosion or deterioration of the tubing due to impurities contained in reagent, gas, air or
cooling water supplied by the user.

(d) Corrosion of the electric circuits or deterioration of the optical elements due to highly
corrosive atmospheric gas.

(e) Failure due to use of software, hardware or spare parts not supplied by the manufacturer.

(f) Failure due to use not described in the manual or improper repair not approved by the
manufacturer.

(g) Failure due to maintenance or repair by other than service personnel qualified by the
manufacturer.

(h) Failure due to relocation or transport conducted not under the supervision of the
manufacturer after the initial installation of the instrument.

(i) Failure due to disassembly, modification or relocation not approved by the manufacturer.

(j) Failure due to acts of God, including fire, earthquake, storm, flood, lightning, social
disturbance, riot, crime, insurrection, terrorism, war (declared or undeclared), radioactive
pollution, contamination with harmful substance, etc.

IMPORTANT - 2
 (k) Failure of the hardware, or damage to the system software, application software, data or
hard disk due to computer virus infection.

(l) After disposal of this instrument, after its resale without prior approval from the
manufacturer, consumable parts, and failure of any part that have reached the end of its
service life.

(m) Failure due to a life-limited part that has exceeded the end of its useful lifetime.

Limitations of Liability

Hitachi High-Tech Science shall not be liable in contract or in tort (including, without limitation,
negligence, strict liability or otherwise) for claims by third parties (other then for bodily injury or
property damage), for economic losses of any kind or for any special, incidental, indirect,
consequential, punitive or exemplary damages, arising in any way out of the performance of, or
failure to perform, this instrument, even if Hitachi High-Tech Science could foresee or has been
advised of the possibility of such damages.

Disclaimer of Liability for Industrial Properties of a Third Party

Hitachi High-Tech Science does not assume any liability for a third party’s complaint regarding
infringement of any patent rights or industrial properties with respect to products manufactured
through use of the equipment supplied by Hitachi High-Tech Science or its related companies
or application of said instrument.

Installation, Relocation and After-sale Technical Service

Installation and Relocation

(a) Installation at delivery shall not be carried out by the user. It shall be carried out by our
sales representative or the engineers who have been trained and qualified for this
purpose by us in order to use the instrument safely and accurately.

(b) Before installation, the user shall make preparations for satisfying the installation
requirements in accordance with this instruction manual.

(c) If relocation becomes necessary after initial installation (delivery), please contact the
dealer from whom you purchased the instrument or our sales representative.

After-sales Service

(a) For after-sale technical service of this instrument, please notify our local sales
representative or service office.

IMPORTANT - 3
 (b) A maintenance & service contract is available for servicing the instrument after the
warranty period has ended (service available at charge).

(c) Maintenance parts and consumables will be supplied during the useful life of this
instrument (7 years). And even though parts and/or units (when available) will continue
to be supplied after the specified useful life has ended (for up to 10 years), they are not
intended to extend the useful life of the instrument. Also, substitutes may be supplied in
the event of discontinuance of manufacture of certain parts or units.

(d) Some main components of the instrument other than maintenance parts and
consumables may not be available in the event of discontinuance of manufacture of the
main unit. If repair cannot be provided in the case of instrument malfunction, we
recommend that you consider discontinuing use and replacing the instrument.

Technical Seminars and Training Courses for Users

We offer technical seminars and training courses at either our or user’s facilities to ensure
proper and safe operation of the analytical instrument to its full performance. For further
information, contact our sales representative. (Applicants will be charged.)

Estimated Life time of the Instrument

This instrument has a useful service life of seven (7) years after the date of initial use
(installation), which is estimated under the condition that periodic maintenance, checkup,
replacement of life-limited parts, and repair of worn parts are carried out as specified in the
instruction manual.

IMPORTANT - 4
Caution on Disposal of Instrument

Although at present the instrument does not use materials that would directly cause
environmental disruption, the environmental protection laws and regulations may be revised or
amended. When planning to dispose of the instrument, therefore, be sure to check the latest
issues of the relevant laws and regulations or consult our sales representative.

Disposal of This Instrument and Its Parts

In the present design, this instrument does not use materials that would directly cause
environmental harm. Note, however, that the environmental protection laws and regulations
may be revised or amended. Therefore, be sure to consult with your local Hitachi
High-Technologies sales or service representative when planning disposal of this instrument or
its parts and accessories.
When discarding or recycling the used PC, it is the user’s responsibility to delete all data from
the hard drive.
When discarding the chargeable battery mounted on the PC, refer waste disposal treatment
servicing to an authorized waste collection agent, or send the battery for recycling after
providing insulation on its positive and negative terminals with adhesive vinyl tape or the like.

IMPORTANT - 5
Information for Users on WEEE (only for EU Countries)

This symbol is in compliance with the Waste Electrical and

Electronic Equipment directive 2012/19/EU (WEEE).
This symbol on the product indicates the requirement NOT to
dispose of the equipment as unsorted municipal waste, but use the
return and collection systems available.

Information on Disposal for Users

1. In the European Union

If you need to discard this product or discard user serviceable parts:

Please contact your local sales representative or distributor who will inform you of the recycle of
the product. You might be charged for the costs arising from take-back and recycling.

2. In other Countries outside the EU

If you wish to discard this product, please contact your local authorities and ask for the correct
method of disposal.

Other Precautions

Handling of Chemicals and Samples

(a) The user is responsible for following relevant laws and regulations in handling, storage and
disposal of chemicals and samples used in analytical operation with this instrument.

(b) Reagents, standard solutions and accuracy-control samples shall be handled, stored and
discarded as instructed by the respective suppliers.

(c) Physiologically or biologically active samples are not applicable to this instrument because
of possible infection with bacteria or viruses.

IMPORTANT - 6
Disturbance by Electromagnetic Wave

This instrument conforms to Class A in the EN standards EN61326-1 (2013).

Avoid installing this instrument near equipment whose data will be affected by
electromagnetic noise within the permissible range of this standard.
Also, data of this instrument may be affected by electromagnetic noise or the instrument itself
may malfunction. In the room where this instrument is installed, the following electric
devices must not be brought: Devices which emit radio waves such as mobile phone,
transceiver, wireless telephone and similar small-power device.

IMPORTANT - 7
 CONTENTS

PREFACE ............................................................................................................... 1

ABOUT THIS MANUAL .......................................................................................... 1

IMPORTANT ..................................................................................... IMPORTANT-1

Warranty on Product ................................................................ IMPORTANT-1
Installation, Relocation and After-sale Technical Service .... IMPORTANT-3
Technical Seminars and Training Courses for Users ........... IMPORTANT-4
Estimated Life time of the Instrument..................................... IMPORTANT-4
Caution on Disposal of Instrument ......................................... IMPORTANT-5
Disposal of This Instrument and Its Parts .............................. IMPORTANT-5
Information for Users on WEEE (only for EU Countries) ...... IMPORTANT-6
Other Precautions..................................................................... IMPORTANT-6

1. OVERVIEW ............................................................................................................ 1-1

1.1 Sample Racks for Standard Autosampler .................................................. 1-1
1.2 Sample Racks for Autosampler Equipped with Thermostat .................... 1-1

2. FUNCTIONS ........................................................................................................... 2-1

2.1 Explanation of Each Control Knob and Switch.......................................... 2-1
2.2 Application.................................................................................................... 2-1
2.3 Specifications ............................................................................................... 2-1
2.3.1 4 mL  72 Type for Standard Autosampler (892-0232) .................... 2-1
2.3.2 1 mL  195 Type for Standard Autosampler (892-0234) .................. 2-1
2.3.3 2 Microplate Type for Standard Autosampler (892-0236) ................. 2-1
2.3.4 4 mL  72 Type for Autosampler with Thermostat (892-0242) ......... 2-2
2.3.5 1 mL  195 Type for Autosampler with Thermostat (892-0244) ....... 2-3
2.3.6 2-microplate Type for Autosampler with Thermostat (892-0246) ...... 2-4

3. OPERATION .......................................................................................................... 3-1

3.1 Preparatory Operations ............................................................................... 3-1
3.2 Measurement ............................................................................................... 3-1
3.2.1 Basics of Operation.......................................................................... 3-1
3.2.2 Basic Operations of Microplate Rack ............................................... 3-2
3.2.3 Analyzing Operation ......................................................................... 3-5

APPENDIX ................................................................................................. APPENDIX 1-1

APPENDIX 1. INSTALLATION ......................................................... APPENDIX 1-1
1.1 Unpacking ....................................................................... APPENDIX 1-1
1.2 Assembling ...................................................................... APPENDIX 1-1
1.2.1 Setting of Vial Detecting Function.................................... APPENDIX 1-1
1.2.2 Setting of Rack Parameters ............................................ APPENDIX 1-1
1.2.3 Check of Rack Parameters ............................................. APPENDIX 1-6
1.2.4 Setting for Temperature Calibration................................. APPENDIX 1-7

-i-
 1. OVERVIEW

This rack is an optional accessory of Chromaster 5210/5260

autosampler and is designed to be mounted in this autosampler
for using the specific-size sample vials. For details of the
Chromaster 5260 autosampler to be coupled with this rack, refer
to the instruction manual of this autosampler.
Tables 1-1 and 1-2 list the optional sample racks for the
Chromaster 5210/5260.

1.1 Sample Racks for Standard Autosampler

These racks are used when the autosampler is not equipped

with a thermostat.

Table 1-1 Optional Sample Racks for

Standard Autosampler

Part No. Part Name Remarks

892-0232 Sample rack for 4 mL  72 (vials)
892-0234 Sample rack for 1 mL  195
892-0236 Sample rack for 2 microplates Deep-well plate for
96 or 384 wells
cannot be used.

1.2 Sample Racks for Autosampler Equipped with Thermostat

These racks are used when the autosampler is equipped with a

thermostat.

Table 1-2 Optional Sample Racks for Autosampler with

Thermostat

Part No. Part Name Remarks

892-0242 Sample rack for 4 mL  72 (vials)
892-0244 Sample rack for 1 mL  195
892-0246 Sample rack for 2 microplates Deep-well plate for
96 or 384 wells
cannot be used.

1-1
 2. FUNCTIONS

2.1 Explanation of Each Control Knob and Switch

Refer to the instruction manual of Chromaster 5210/5260.

2.2 Application

This optional accessory is to be mounted in the Chromaster

5210/5260 autosampler for placing the specific-size sample vials
in the autosampler.

2.3 Specifications

2.3.1 4 mL  72 Type for Standard Autosampler (892-0232)

(1) Applicable autosampler : Chromaster 5210/5260

(2) Number of mountable

sample vials : 72

(3) Applicable sample vial : 4 mL (15 mm dia.  46 mm H)

2.3.2 1 mL  195 Type for Standard Autosampler (892-0234)

(1) Applicable autosampler : Chromaster 5210/5260

(2) Number of mountable

sample vials : 195

(3) Applicable sample vial : 1 mL (8 mm dia.  40 mm H)

2.3.3 2 Microplate Type for Standard Autosampler (892-0236)

(1) Applicable autosampler : Chromaster 5210/5260

(2) Number of mountable

microplates : 2

(3) Applicable microplate : 96 or 384-well microplate

2-1
2.3.4 4 mL  72 Type for Autosampler with Thermostat (892-0242)

(1) Applicable autosampler : Chromaster 5210/5260 with

thermostat

(2) Settable temperature

range : 1 to 45 C (in increments of
1 C)

(3) Controllable temperature

range : [Ambient temperature - 21 C]
to [ambient temperature +
25 C] and within the settable
range (humidity 60% and
sample vial 4 mL)

(4) Temperature setting

accuracy : 3 C
(when measuring liquid
temperature at the bottom of
central sample vial (4 mL) at
set temperature 4 C and 40 C,
ambient temperature 25 C and
relative humidity 60%.
Sample freezing unallowable)

(5) Temperature
distribution : 2 C
(when measuring liquid
temperature at the bottom of
central and 4-corner sample
vials (4 mL) at set temperature
4 C and 40 C, ambient
temperature 25 C and relative
humidity 60%.
Sample freezing unallowable)

(6) Number of mountable

sample vials : 72 (4 mL vials)

(7) Applicable sample vial : 4 mL (15 mm dia.  46 mm H)

2-2
2.3.5 1 mL  195 Type for Autosampler with Thermostat (892-0244)

(1) Applicable autosampler : Chromaster 5210/5260 with

thermostat

(2) Settable temperature

range : 1 to 45 C (in increments of
1 C)

(3) Controllable temperature

range : [Ambient temperature - 21 C]
to [ambient temperature +
25 C] and within the settable
range (humidity 60% and
sample vial 1 mL)

(4) Temperature setting

accuracy : 3 C
(when measuring liquid
temperature at the bottom of
central sample vial (1 mL) at
set temperature 4 C and 40 C,
ambient temperature 25 C and
relative humidity 60%.
Sample freezing unallowable)

(5) Temperature distribution : 2 C

(when measuring liquid
temperature at the bottom of
central and 4-corner sample
vials (1 mL) at set temperature
4 C and 40 C, ambient
temperature 25 C and relative
humidity 60%.
Sample freezing unallowable)

(6) Number of mountable

sample vials : 195 (1 mL vials)

(7) Applicable sample vial : 1 mL (8 mm dia.  40 mm H)

2-3
2.3.6 2-microplate Type for Autosampler with Thermostat (892-0246)

(1) Applicable autosampler : Chromaster 5210/5260 with

thermostat
(2) Settable temperature
range : 1 to 45 C (in increments of
1 C)
(3) Controllable temperature
range : [Ambient temperature - 15 C]
to [ambient temperature +
20 C] and within the settable
range (humidity 60% and
microplate)

(4) Temperature setting

accuracy : 4 C
(when measuring liquid
temperature at the bottom of
central sample well (in
microplate) at set temperature
10 C and 40 C, ambient
temperature 25 C and relative
humidity 60%.
Sample freezing unallowable)

(5) Temperature distribution: 3 C

(when measuring liquid
temperature at the bottom of
central and 4-corner sample
wells (in each microplate) at set
temperature 10 C and 40 C,
ambient temperature 25 C and
relative humidity 60%.
Sample freezing unallowable)
(6) Number of mountable
microplates : 2

(7) Applicable microplate : 96 or 384-well microplate

2-4
NOTE: Temperature is settable within a range from 1 to
45 C, but the actually attainable temperature
varies significantly with the ambient conditions
(ambient temperature and humidity).
Therefore, the controllable temperature range is
given as a separate specification item.

NOTE: Be sure to use the sample vials, caps and septa

recommended by Hitachi High-Tech Science.
If other products are used, the instrument might
not provide the original performance.

2-5
 3. OPERATION

3.1 Preparatory Operations

Set the vial detecting function, and set and check rack
parameters, referring to “APPENDIX 1. INSTALLATION.”

3.2 Measurement

3.2.1 Basics of Operation

(1) Operation of Sample Rack

For details, refer to the instruction manual (Section 3) of

Chromaster 5210/5260 autosampler.

(2) Operation of Sample Rack for Autosampler with

Thermostat

For details, refer to the instruction manual (Section 3) of

Chromaster 5210/5260 autosampler.

(3) Operation of Microplate

For details, refer to the instruction manual (Section 6) of

Chromaster 5210/5260 autosampler.

(4) Operation of Vial Detecting Function

When an optional sample rack for microplates is used, the

vial detecting function does not work. So, before using
this type of sample rack, inactivate the vial detecting
function. For details, refer to the instruction manual
(Section 3) of Chromaster 5210/5260 autosampler.

3-1
3.2.2 Basic Operations of Microplate Rack

(1) Setting of Microplate

(a) Raise the rack cover.

(b) Fit a microplate to the sample rack.

(c) Push in the rack cover.

(d) Mount the sample rack to the rack holder.

Fit a microplate to
the sample rack.

Fig. 3-1 Setting and Removal of Microplate

NOTE: Push in the rack cover securely. If the rack cover

is not pushed in completely, the instrument might
malfunction.

3-2
(2) Removal of Microplate

(a) Remove the sample rack from the rack holder.

(b) Raise the rack cover.

(c) Take out the microplate from the sample rack.

(3) Use in Hot and Humid Environment

If the microplate rack for the autosampler equipped with a

thermostat is used in a hot and humid environment, the
microplate may become difficult to remove because it may
come in close contact with the aluminum block due to
condensation. In this case, utilize a blade-edge
screwdriver or the like for removing the microplate.

Aluminum block

Fig. 3-2 Microplate Rack

3-3
(4) Temperature Calibration

When the microplate rack for the autosampler with a

thermostat is used in thermostatic control, a difference
occurs between the indicated temperature of thermostat
and the actual sample temperature.
Therefore, temperature calibration is required using the
offset values shown in Fig. 3-3 as a reference. (Set a
correction value (offset value), referring to “3.2.2 Analyzing
Operation, (7) Thermostat, (b) Temperature calibration” in
Section 3 of the instruction manual of Chromaster
5210/5260 autosampler.)
For example, input +1.5 C as a correction value (offset
value) when thermostat activating temperature is set at
10 C for operation at room temperature 20 C.

4.5

1.5
Offset value [C]

0
-30 -20 -10 0 10 20 30
-1.5

-3

-4.5
(Set temperature - room temperature)[C]

Fig. 3-3 Relationship between Set Temperature,

Room Temperature and Offset Value

NOTE: The offset values (Fig. 3-3) are set as typical

values for the microplates recommended by
Hitachi High-Tech Science. When a stricter
temperature control is needed, measure the liquid
temperature in the wells after reaching the set
temperature and temperature calibration should be
carried out so that the aimed-at temperature can
be reached.

3-4
 NOTE: If any microplate other than recommended by
Hitachi High-Tech Science is used, offset values
may differ significantly from the above values.
Furthermore, the thermostatic controllability
intrinsic to the instrument will be unavailable.

3.2.3 Analyzing Operation

For details, refer to the instruction manual of Chromaster

5210/5260 autosampler.

3-5
 APPENDIX

APPENDIX 1. INSTALLATION

1.1 Unpacking

NOTE: It is not permitted for the user to carry out

installation at the time of delivery. For ensuring
safety and high accuracy in operation of the
instrument, installation of the instrument shall be
carried out by or under supervision of qualified
service personnel of the manufacturer or its
authorized service agent. Before installation,
however, the user shall make adequate
preparations for installing the instrument, referring
to this instruction manual. If relocation of the
instrument becomes necessary after delivery,
consult your dealer or nearest Hitachi High-
Technologies service representative in order to
avoid possible trouble involved in relocation.

1.2 Assembling

This accessory needs to be installed in the Chromaster

5210/5260 autosampler. Installation should be carried out in
the procedure given below.

1.2.1 Setting of Vial Detecting Function

When an optional sample rack for microplates is used, the vial

detecting function does not work. So, before using this type of
sample rack, inactivate the vial detecting function.

1.2.2 Setting of Rack Parameters

Input appropriate rack parameters for the optional sample rack

to be used, referring to “3.2.2 Analyzing Operation” in Section 3
of the instruction manual of Chromaster 5210/5260 autosampler.
For using an optional sample rack for microplates, input rack
parameters for each microplate as shown in Appended Fig. 2-2.

Up to 16 rack parameters are saved to the Chromaster

5210/5260 Autosampler as rack cords (No.1 to 16).
Select the rack code which a suitable parameter is set to choose
a rack parameter.

APPENDIX 1 - 1
 The rack code is indicated in the direction of each rack.
(Refer to the instruction manual for the Chromaster 5210/5260
Autosampler Section 3 "3.2.2 Analyzing Operation ", Table 3-1
Rack Parameters).

X2 Input range of each rack parameter

X1 : X coordinate of first vial
55.0 to 180.0 mm
Y1 : Y coordinate of first vial
0.0 to 155.0 mm
X2 : X coordinate of last vial
X1 55.0 to 180.0 mm
Y2 : Y coordinate of last vial
0.0 to 155.0 mm
Z : Height of needle
0.0 to 53.0 mm
Y2 Nx : Number of vials in X direction
1 to 40
Ny : Number of vials in Y direction
1 to 40
y PATTERN CODE: 1 to 8
Y1

Needle exchange position Wash port Injection port

(57.1, 0.0, 10.0) (29.7, 0.0, 53.0), (0.0, 0.0, 40.0),
idle position PI home position

Appended Fig. 2-1 Rack Parameters

NOTE: Rack parameters to be input should be appropriate

for the optional sample rack. If rack parameters
are inappropriate, an error may occur or the
instrument would become faulty.

NOTE: Rack parameters have been set for the sample

vials (or microplates) recommended by Hitachi
High-Tech Science. For any sample vial other
than recommended by Hitachi High-Tech Science,
the rack parameters may not be appropriate.
Input appropriate rack parameters, referring to
“3.2.2 Analyzing Operation, (3)(b)<2> Rack
parameter setting procedure” in Section 3 of the
instruction manual of Chromaster 5210/5260
autosampler.

APPENDIX 1 - 2
 X2

X1

Microplate 2

Y22 Y21 Microplate 1

Y12 Y11

Needle exchange position Wash port Injection port

(57.1, 0.0, 10.0) (29.7, 0.0, 53.0), (0.0, 0.0, 40.0),
idle position PI home position

Rack parameters
X1 : X coordinate of first vial
55.0 to 180.0 mm
Y11 : Y coordinate of first vial in microplate 1
0.0 to 155.0 mm
Y21 : Y coordinate of first vial in microplate 2
0.0 to 155.0 mm
X2 : X coordinate of last vial
55.0 to 180.0 mm
Y12 : Y coordinate of last vial in microplate 1
0.0 to 155.0 mm
Y22 : Y coordinate of last vial in microplate 2
0.0 to 155.0 mm
Z : Height of needle
0.0 to 53.0 mm
Nx : Number of vials in X direction
1 to 40
Ny : Number of vials in Y direction
1 to 40
PATTERN CODE: 1 to 8

Appended Fig. 2-2 Rack Parameters for Optional

Microplate Rack

APPENDIX 1 - 3
(1) 2-microplate type for standard autosampler (part No. 892-
0236) and 2-microplate type for autosampler equipped with
thermostat (part No. 892-0246)

(a) When using 96-well microplate:

X1 68.5
Y11 2.8
Y21 89.1
X2 167.5
Y12 65.8
Y22 152.1
Z 42.0
Nx 12
Ny 8

Microplate Rack Code

1 14
2 13

(b) When using 384-well microplate:

X1 66.1
Y11 0.5
Y21 87.0
X2 169.6
Y12 68.0
Y22 154.5
Z 42.0
Nx 24
Ny 16

Microplate Rack Code

1 16
2 15

APPENDIX 1 - 4
(2) 4 mL  72 type for standard autosampler (part No. 892-
0232) and 4 mL  72 type for autosampler equipped with
thermostat (part No. 892-0242)

X1 59.0
Y1 15.4
X2 176.6
Y2 149.8
Z 48.0
Nx 8
Ny 9

Rack Code
11

(3) 1 mL  195 type for standard autosampler (part No. 892-

0234) and 1 mL  195 type for autosampler equipped with
thermostat (part No. 892-0244)

X1 57.2
Y1 8.6
X2 178.5
Y2 150.0
Z 48.0
Nx 13
Ny 15

Rack Code
12

APPENDIX 1 - 5
1.2.3 Check of Rack Parameters

After setting rack parameters, make sure those parameters have

been set properly, referring to “3.2.2 Analyzing Operation,
(3)(b)<2> Rack parameter setting procedure” in Section 3 of the
instruction manual of Chromaster 5210/5260 autosampler.

NOTE: For preventing the instrument from malfunctioning,

be sure to check the set rack parameters.

NOTE: Rack parameters have been set as typical values

for the sample vials (or microplates) recommended
by Hitachi High-Tech Science. Before using an
optional sample rack, check the rack parameters
so that the optimum ones can be set. For using
any sample vial, cap and septum recommended by
Hitachi High-Tech Science, the rack parameters
may not be appropriate. In this case, input and
use appropriate rack parameters, referring to
“3.2.2 Analyzing Operation, (3)(b)<2> Rack
parameter setting procedure” in Section 3 of the
instruction manual of Chromaster 5210/5260
autosampler.

APPENDIX 1 - 6
1.2.4 Setting for Temperature Calibration

When the absolute temperature control of samples is required,

measure sample temperature after reaching the set temperature
and carry out temperature calibration so that the aimed-at
temperature can be reached. (Set a correction value (offset
value), referring to “3.2.2 Analyzing Operation, (7) Thermostat,
(b) Temperature calibration” in Section 3 of the instruction
manual of Chromaster 5210/5260 autosampler.)
In particular, when the microplate rack for the autosampler with a
thermostat is used, a difference occurs between the indicated
temperature of thermostat and the actual sample temperature.
Therefore, temperature calibration is required using the offset
values shown in Fig. Appended Fig. 2-3 as a reference.

4.5

1.5
Offset value [C]

0
-30 -20 -10 0 10 20 30
-1.5

-3

-4.5
(Set temperature - room temperature)[C]

Appended Fig. 2-3 Relationship among Set Temperature,

Room Temperature and Offset Value

NOTE: The offset values (Appended Fig. 2-3) are set as

typical values for the microplates recommended by
Hitachi High-Tech Science. When a stricter
temperature control is needed, measure the liquid
temperature in the wells after reaching the set
temperature and temperature calibration should be
carried out so that the aimed-at temperature can
be reached.

APPENDIX 1 - 7
NOTE: If any microplate other than recommended by
Hitachi High-Tech Science is used, offset values
may differ significantly from the above values.
Furthermore, the thermostatic controllability
intrinsic to the instrument will be unavailable.

APPENDIX 1 - 8