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    Journal Pone 0206678 s001

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    Ademilson Lima
    The document describes the procedure for preparing sensor patches coated with a palladium dye (Pd-TFPP) embedded in a Teflon AF 1600 matrix. Glass substrates are first silanized using sodium hydroxide, ethanol, and Aquaphobe CF to make their surfaces hydrophobic. The silanized substrates are then assembled into a sensor patch configuration. Pd-TFPP dye is dissolved into octafluorotoluene along with Teflon AF 1600 and applied to the sensor patches in thin layers. The dried sensor patches have a shelf life of at least two years and continuous operational life of several months. The coating method can also be used for laboratory glassware.

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    Journal Pone 0206678 s001

    Uploaded by

    Ademilson Lima
    22 views2 pages
    The document describes the procedure for preparing sensor patches coated with a palladium dye (Pd-TFPP) embedded in a Teflon AF 1600 matrix. Glass substrates are first silanized using sodium hydroxide, ethanol, and Aquaphobe CF to make their surfaces hydrophobic. The silanized substrates are then assembled into a sensor patch configuration. Pd-TFPP dye is dissolved into octafluorotoluene along with Teflon AF 1600 and applied to the sensor patches in thin layers. The dried sensor patches have a shelf life of at least two years and continuous operational life of several months. The coating method can also be used for laboratory glassware.

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    journal.pone.0206678.s001

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    The document describes the procedure for preparing sensor patches coated with a palladium dye (Pd-TFPP) embedded in a Teflon AF 1600 matrix. Glass substrates are first silanized using sodium hydroxide, ethanol, and Aquaphobe CF to make their surfaces hydrophobic. The silanized substrates are then assembled into a sensor patch configuration. Pd-TFPP dye is dissolved into octafluorotoluene along with Teflon AF 1600 and applied to the sensor patches in thin layers. The dried sensor patches have a shelf life of at least two years and continuous operational life of several months. The coating method can also be used for laboratory glassware.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    22 views2 pages

    Journal Pone 0206678 s001

    Uploaded by

    Ademilson Lima
    The document describes the procedure for preparing sensor patches coated with a palladium dye (Pd-TFPP) embedded in a Teflon AF 1600 matrix. Glass substrates are first silanized using sodium hydroxide, ethanol, and Aquaphobe CF to make their surfaces hydrophobic. The silanized substrates are then assembled into a sensor patch configuration. Pd-TFPP dye is dissolved into octafluorotoluene along with Teflon AF 1600 and applied to the sensor patches in thin layers. The dried sensor patches have a shelf life of at least two years and continuous operational life of several months. The coating method can also be used for laboratory glassware.

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    S1 Sensor patch preparation procedure

    The Pd-TFPP dye in this work was embedded into a Teflon AF 1600 matrix. This is similar to the

    procedure used in (1), but he procedure was custom-developed to use more available components.

    Teflon AF 1600 is hydrophobic and glass substrates are originally hydrophilic, so the glass

    substrates were silanized by:

    1. Cleaning them thoroughly using ethanol and deionized water.

    2. Shaking in a container with 2.5 M NaOH for 24 hours.

    3. Sonicating in a sonic bath in deionized water for 10 minutes.

    4. Immersing in 80% ethanol for 5 minutes.

    5. Dipping into a container of 2% Aquaphobe CF (from Gelest, Morrisville, Pennsylvania,

    USA) in isopropyl alcohol for 15 minutes.

    6. Cleaning the substrates by dipping into ethanol.

    7. Storing in a jar with dessicant.

    The silanized sensor patches were assembled into the final configuration with the metal

    holder and the four magnets (Fig 4 in the main article). To coat the patches with the dye, 160 mg

    of PTFE AF 1600 (from Sigma-Aldrich, St. Louis, Missouri, USA) was dissolved by shaking for

    4 days in octafluorotoluene (from Sigma-Aldrich, St. Louis, Missouri, USA) in a fume hood on an

    orbital shaker. After this, 0.5 mg of Pd-TFPP (from Sigma-Aldrich, St. Louis, Missouri, USA) was

    added to the solution.

    Six microliters of the dye solution were transferred onto the silanized substrate (to the

    ground side of the glass pieces), already integrated to the holder, in two 15-microliter layers using

    5 microliter capillary pipettes (Microcap 1-000-0050 Microliter Pipets from Drummond Scientific,

    Broomall, Pennsylvania, U.S.A.). The pipettes were also silanized using the procedure described

    1
    for glass substrate. The metal holder of the sensor patch acted as a stencil and held the liquid in

    place while it dried into a thin film. Between the two 15 µl applications, the dye was allowed to

    dry in a fume hood for a couple of minutes. The dried sensor patches can be immediately used and

    have at least a two year-long shelf life in standard laboratory conditions and several months of

    continuous operational life. We did not quantify the operational lifetime, but the longest

    continuous test run of the sensor was 4 months at 22°C and the sensor patch remained usable

    during this entire time. Tests performed at higher temperatures indicate faster degradation.

    The method described has also been successfully applied for directly spot-coating

    laboratory glassware (both silanization and dye-coating). The coated glassware also survived

    autoclaving. Still, using sensor patches has been more convenient in the laboratory conditions.

    References

    1. Lehner P, Larndorfer C, Garcia-Robledo E, Larsen M, Borisov SM, Revsbech N-P, et al.


    LUMOS - a sensitive and reliable optode system for measuring dissolved oxygen in the
    nanomolar range. D’Auria S, editor. PLOS ONE. 2015 Jun 1;10(6):e0128125.

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