Photosynthesis in Higher Plants: Class: 11 Sub: Biology Chapter - 13

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CLASS: 11 SUB: BIOLOGY CHAPTER -13

PHOTOSYNTHESIS IN HIGHER PLANTS


Photosynthesis is a physico-chemical process by which green plants use light energy to synthesize
organic compounds (sugar).

6CO2 +12H2O C6H12O6 + 6H2O + 6O2

IMPORTANCE OF PHOTOSYNTHESIS:

1) It is the primary source of all food in earth.


2) Release of oxygen into the atmosphere.

SITE OF PHOTOSYNTHESIS:
 Photosynthesis occurs in mesophyll cell of the green leaves in a cell organelle called chloroplast.
 Within the chloroplast there is a membranous system consisting of grana, the stroma lamellae
and the fluid stroma.
 The membrane system trap the light energy and synthesizes ATP and NADPH. This set of
reaction reaction which depends on light is called light reaction.
 In stroma, enzymatic reactions incorporate CO2 into the plant leading to the synthesis of sugar
which in turn forms starch. This set of reactions which are not directly dependent on light but
are dependant on the products of light reactions. (ATP and NADPH) is called dark reaction.
OVERVIEW OF PHOTOSYNTHESIS

PIGMENTS INVOLVED IN PHOTOSYNTHESIS


Four pigments are involved in photosynthesis
 Chlorophyll a is the main pigment .
 Pigments like chlorophyll b , xznthophylls and carotenoids are called accessory pigments.
 Accessory pigments absorb light and transport the energy to chlorophyll a.
LIGHT REACTION (PHOTOCHEMICAL PHASE)
 Light reactions include light absorption, water splitting, oxygen release and the formation of
high energy chemical intermediates, ATP and NADPH.
 The pigments are organized into two discrete photo chemical light harvesting complexes within
the photosystem I (PS I) and photosystem II (PS II)
 In PS I the reaction centre chloroohyll a has an absorotion peak at 700 nm, hence is called P700
while the PS II has an absorption peak at 680 nm and is called P680.
THE ELECTRON TRANSPORT
 Absorption of light by PS II causes the electron to move to excited state.
 The excited electrons are picked up by an electron acceptor which passes them to an electron
transport system consisting of cytochromes.
 The electrons are then passed on to photosystem I.
 Just like in photosystem II, absorption of light by PS I causes the electrons to move to excited
state.
 The excited electrons are passed on to another cytochrome with greater electron affinity.
 The electrons are then passed on to energy rich molecules called NADP+ which after receiving
the electrons gets reduced to NADPH + H+.
 Z scheme – This whole scheme of transfer of electrons from PS II to cytochrome to PS I to
another cytochrome to NADP+ to form NADPH + H+ is called the Z scheme..

SPLITTING OF WATER
 Water is split into hydrogen ion(H+), oxygen (o) and electrons
2H2o 4H+ + o2 + 4e-
 The electrons released from splitting of water is used to replace the electrons lost by
photosystem II.
CYCLIC AND NON-CYCLIC PHOTO-PHOSPHORYLATION
 The process of formation of ATP from ADP and inorganic phosphate (iP) in chloroplast in the
presence of sun light is called photo-phosphorylation.
 Photo-phosphorylation is of two types :
1. Non-cyclic photo-phosphorylation
2. Cyclic photo-phosphorylation

Non-cyclic photo-phorylation Cyclic photo-phosphorylation


Both PSI and PSII are involved. Only PS I is involved.
Electrons are not recycled. Electrons released by PS I is recycled by PS I.
Both ATP and NADPH are produced. Only ATP is produced.
Photolysis of water takes place. No photolysis
Oxygen is released. No oxygen released.

CHEMIOSMOTIC HYPOTHESIS:
 The theory which explains how ATP is synthesized in the chloroplast is chemiostic hypothesis.
 ATP is produced due to the proton gradient created across the membrane.The essential
components required for chemiosmosis are proton pump,proton gradient and ATP synthase.
DARK REACTION ( Biosynthetic phase)

This process occurs in the stroma of the chloroplasts. The following cycles are involved in the
process:

CALVIN CYCLE: This cycle involves the following steps:

Carboxylation: Ribulose -1,5 bisphosphate combines with carbon dioxide to form 3-carbon
compound 3-phosphoglyceric acid. The enzyme RuBisCO is involved in the process.

Reduction: Reduction is series of reaction that leads to formation of glucose. Two molecule of
ATP and two molecules NADPH are required for reduction of one molecules of CO2. Six turn of
this cycle are required for removal of one molecule of glucose from pathway.

Regeneration: Regeneration is the generation of RUBP molecules for the continuation of cycle.
This process requires one molecule of ATP.

IN OUT
SIX CO2 One glucose
18 ATP 18 ADP
12 NADPH 12 NADP
Sucrose,
Starch

Fig : Calvin Cycle

C4 PATHWAY/ HATCH AND SLACK PATHWAY:

 This pathway is operational in plants growing in dry tropical region like Maize, Sugarcane,
Sorghum etc.
 The first stable product is a 4-carbon compound Oxaloacetic acid (OAA).
 The primary CO2 acceptor is 3C Phosphoenol pyruvate(PEP) present in mesophyll cell and
enzyme involved is PEP carboxylase.
 OAA is converted to Malic acid which is transported to bundle sheath cells.
 In bundle sheath cell, it is broken into CO2 and a 3C molecule.
 The 3C molecule is transported back to the mesophyll where is converted to PEP again, thus
completing the cycle.
 The CO2 released in the bundle sheath cells enters the Calvin cycle, where enzyme RuBisCO is
present that forms sugar.
C3 PLANTS C4 PLANTS
The leaves do not have kranz anatomy. The leaves show Kranz anatomy.
RUBP is the first acceptor of CO2. PEP is the first acceptor of CO2.
PGA is the first stable product. OAA is the first stable product.
Photosynthesis occurs in mesophyll cells. Photosynthesis occurs in both mesophyll cells and
bundle sheath cells.
Photorespiration occurs. Photorespiration does not occur.

PHOTORESPIRATION:

Photorespiration is a process that lowers the efficiency of photosynthesis in C3 plants. In these


plants, oxygen binds with RuBisCO during photosynthesis, which results in reduced carbon
dioxide fixation. Additionally, this process does not result in the synthesis of sugars nor ATP or
NADPH.
FACTORS AFFECTING PHOTOSYNTHESIS:
1. Light
2. Carbon dioxide concentration
3. Temperature
4. Water
MODEL QUESTIONS WITH ANSWER

Q1. Photosynthesis occurs in :


a) Chloroplast b) Nucleus c) Mitochondria d) Golgi body
Ans: a) Chloroplast
Q2. Kranz anatomy is found in the leaves of:
a) C3 Plants b) C4 Plants c) both d) none
Ans: b) C4 Plants
Q3. Photosynthetically active radiation (PAR) is represented by the range of wavelength of :
a) 350-450 nm b) 400-700 nm c) 500-600 nm d) 450-950 nm
Ans: b) 400-700 nm
Q4 Where does light reaction take place:
a) Grana b) Stroma c) Cytoplasm d) Ribosome
Ans: a) Grana
Q5 Non-cyclic photophosphorylation results in the formation of :
a) ATP b) ATP and NADPH c) NADPH d) None

Ans: b) ATP and NADPH

Q6 Where does dark reaction take place:


a) Grana b) Stroma lamellae c) Stroma d) all
Ans: c) Stroma
Q7 The first acceptor of CO2 in C4 plants is :
a) Malic acid b) Oxaloacetic acid c) Phosphoenol pyruvate (PEP)
d) Aspartic acid
Ans: C) Phosphoenol pyruvate (PEP)
Q8 The first stable product of C3 cycle is :
a) PGA b) OAA c) PEP d) RUBP
Ans : a) PGA
Q9 Rubisco enzyme in C4 plant is found in :
a) Mesophyll cell b) Mitochondria c) Peroxisome
d) Bundle Sheath cell
Ans: d) Bundle Sheath cell
Q10 In PS II the reaction centre is :
a) P 700 b) P 680 c) P 400 d) P 650
Ans: b) P 680
Q11 Which is the primary acceptor of CO2 in C3 plants:
a) RUBP b) PEP c) OAA d)PGA
Ans: a) RUBP
Q12 Photorespiration occurs in :
a) C4 plants b)C3 plants c) Both d) None
Ans : C3 plants
Q13 During photosynthesis oxygen is evolved from :
a) Water b) Sunlight c) CO2 d)Chlorophyll
Ans: a) water
Q14 Which element is required for photolysis of water:
a) Mg b) Mn c) Fe d) Zn
Ans: B) Mn
Q15 Law of limiting factor was proposed by:
a) Blackman b) Hill c) Arnold d) Engelman
Ans: a) Blackman
Prepared by
Dolly Dey
PGT Biology
S S +2 High School Patamda
East Singhbhum

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