Photosynthesis in Higher Plants: Class: 11 Sub: Biology Chapter - 13
Photosynthesis in Higher Plants: Class: 11 Sub: Biology Chapter - 13
Photosynthesis in Higher Plants: Class: 11 Sub: Biology Chapter - 13
IMPORTANCE OF PHOTOSYNTHESIS:
SITE OF PHOTOSYNTHESIS:
Photosynthesis occurs in mesophyll cell of the green leaves in a cell organelle called chloroplast.
Within the chloroplast there is a membranous system consisting of grana, the stroma lamellae
and the fluid stroma.
The membrane system trap the light energy and synthesizes ATP and NADPH. This set of
reaction reaction which depends on light is called light reaction.
In stroma, enzymatic reactions incorporate CO2 into the plant leading to the synthesis of sugar
which in turn forms starch. This set of reactions which are not directly dependent on light but
are dependant on the products of light reactions. (ATP and NADPH) is called dark reaction.
OVERVIEW OF PHOTOSYNTHESIS
SPLITTING OF WATER
Water is split into hydrogen ion(H+), oxygen (o) and electrons
2H2o 4H+ + o2 + 4e-
The electrons released from splitting of water is used to replace the electrons lost by
photosystem II.
CYCLIC AND NON-CYCLIC PHOTO-PHOSPHORYLATION
The process of formation of ATP from ADP and inorganic phosphate (iP) in chloroplast in the
presence of sun light is called photo-phosphorylation.
Photo-phosphorylation is of two types :
1. Non-cyclic photo-phosphorylation
2. Cyclic photo-phosphorylation
CHEMIOSMOTIC HYPOTHESIS:
The theory which explains how ATP is synthesized in the chloroplast is chemiostic hypothesis.
ATP is produced due to the proton gradient created across the membrane.The essential
components required for chemiosmosis are proton pump,proton gradient and ATP synthase.
DARK REACTION ( Biosynthetic phase)
This process occurs in the stroma of the chloroplasts. The following cycles are involved in the
process:
Carboxylation: Ribulose -1,5 bisphosphate combines with carbon dioxide to form 3-carbon
compound 3-phosphoglyceric acid. The enzyme RuBisCO is involved in the process.
Reduction: Reduction is series of reaction that leads to formation of glucose. Two molecule of
ATP and two molecules NADPH are required for reduction of one molecules of CO2. Six turn of
this cycle are required for removal of one molecule of glucose from pathway.
Regeneration: Regeneration is the generation of RUBP molecules for the continuation of cycle.
This process requires one molecule of ATP.
IN OUT
SIX CO2 One glucose
18 ATP 18 ADP
12 NADPH 12 NADP
Sucrose,
Starch
This pathway is operational in plants growing in dry tropical region like Maize, Sugarcane,
Sorghum etc.
The first stable product is a 4-carbon compound Oxaloacetic acid (OAA).
The primary CO2 acceptor is 3C Phosphoenol pyruvate(PEP) present in mesophyll cell and
enzyme involved is PEP carboxylase.
OAA is converted to Malic acid which is transported to bundle sheath cells.
In bundle sheath cell, it is broken into CO2 and a 3C molecule.
The 3C molecule is transported back to the mesophyll where is converted to PEP again, thus
completing the cycle.
The CO2 released in the bundle sheath cells enters the Calvin cycle, where enzyme RuBisCO is
present that forms sugar.
C3 PLANTS C4 PLANTS
The leaves do not have kranz anatomy. The leaves show Kranz anatomy.
RUBP is the first acceptor of CO2. PEP is the first acceptor of CO2.
PGA is the first stable product. OAA is the first stable product.
Photosynthesis occurs in mesophyll cells. Photosynthesis occurs in both mesophyll cells and
bundle sheath cells.
Photorespiration occurs. Photorespiration does not occur.
PHOTORESPIRATION: