Portable Optical Dissolved Oxygen Meter Instruction Manual: Apera Instruments, LLC

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DO850

Portable Optical Dissolved Oxygen Meter


Instruction Manual

APERA INSTRUMENTS, LLC


aperainst.com
v3.0

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Contents
1 Overview .................................................................................................................. - 3 -
1.1 Luminescent optical sensor. ........................................................................................................... - 3 -
1.2 Intelligent Instrumentation ............................................................................................................ - 3 -
2 Technical Specifications ........................................................................................... - 4 -
3 Instructions ............................................................................................................... - 4 -
3.1 LCD Screen ...................................................................................................................................... - 4 -
3.2 Key Operation ................................................................................................................................. - 5 -
3.3 Batteries ......................................................................................................................................... - 6 -
3.4 Instrument Socket .......................................................................................................................... - 6 -
3.5 Reading Stability Mode .................................................................................................................. - 6 -
3.6 Auto Lock Mode ............................................................................................................................. - 6 -
3.7 Backlighting .................................................................................................................................... - 6 -
3.8 Automatic Power-Off ...................................................................................................................... - 6 -
4 Optical Dissolved Oxygen Probe .............................................................................. - 7 -
4.1 Probe Structure .............................................................................................................................. - 7 -
4.2 Probe Maintenance ........................................................................................................................ - 7 -
4.3 Sensor Cap ...................................................................................................................................... - 8 -
5 Preparation for Calibration ........................................................................................ - 8 -
5.1 Dissolved Oxygen Units Selection .................................................................................................. - 8 -
5.3 Temperature Unit Selection............................................................................................................. - 9 -
5.4 Air Pressure Compensation ............................................................................................................. - 9 -
6 Calibration ................................................................................................................ - 9 -
6.1 Saturated Oxygen Calibration ......................................................................................................... - 9 -
6.2 Zero -Oxygen Calibration ................................................................................................................ - 9 -
6.3 Special Notes for Calibration ........................................................................................................ - 10 -
7 Measurement.......................................................................................................... - 10 -
8 Parameter Settings .................................................................................................. - 11 -
9 Complete Kit............................................................................................................ - 13 -
10 Warranty ................................................................................................................ - 13 -
11 Trouble Shooting ................................................................................................... - 14 -

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1 Overview

Thank you for purchasing Apera Instruments DO850 Portable Optical Dissolved Oxygen Meter. The
DO850 measures dissolved oxygen in water using luminescence technology through an optical
sensor and displays data with intelligent instrumentation. Compared to conventional electrochemical
dissolved oxygen meter, the DO850 is more accurate, stable and easier to use.

Before you use the instrument, please carefully read the instruction manual to help you properly
perform tests and maintainenance.

1.1 Luminescent optical sensor.


⚫ Stability and Accuracy: Oxygen is not consumed during measurements. It is not affected by
sample flow rate and thus provides a stable measurement.
⚫ Easy to Use: No electrolytes or membranes are required; frequent calibration is not
necessary.
⚫ Interference-Free: Sensor cap is coated with a light-shielding layer and minimizes the impact
from external light sources. The use of non-chemical sensors helps reduce a variety of heavy
metal ions interference in the aqueous environment with H2S and NH4 and other chemical
substances.
⚫ Long service life: except for mechanical deterioration (such as scratches to the light shielding
layer), the sensor cap has up to 8000 hours of service life.
⚫ Easy to calibrate and maintain. Probe is equipped with a calibration/storage sleeve, which
makes calibration and maintenance more convenient and reliable.

1.2 Intelligent Instrumentation


⚫ Built-in microprocessor chip, featured with Auto. Temperature Compensation, Auto. Air
Pressure Compensation, Auto. Salinity Compensation and parameter setting, auto. power off,
and low power indication.
⚫ Stable reading and automatic locking modes available.
⚫ Clear large-size LCD display with white backlight.
⚫ Meets IP57 waterproof rating; In addition, a rugged instrument suitcase is provided.

Special Notes
⚫ Sensor cap surface coating can not withstand high temperature, so the optical dissolved
oxygen electrode can not test water with temperature over 50 ℃.
⚫ When the electrode is not in use, it should be kept in the storage sleeve and the
sponge in the cap should be kept moist, so that the fluorescent cap will not dry out. If
the sponge is dried out or the probe is exposed in dry air for more than 8 hours, soak
the electrode in tap water for 24~48 hours (see section 4.2), otherwise it may cause
unstable measurements or slow response.
⚫ Before getting readings or performing other operations, wait about 30 seconds after meter is
powered on.

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2 Technical Specifications

Dynamic Range (0-20.00) mg/L (ppm), (0-200.0)%


Resolution 0.01/0.1mg/L (ppm), 0.1/1%
±2% reading or ±2% saturation,
whichever is greater
Accuracy
±2% reading or ±0.2 mg/L,
Dissolved whichever is greater
Oxygen Response Time ≤30 s (25℃, 90% response)
Calibration Points Saturation Point & Zero Oxygen
Temperature Compensation Automatic, (0 to 50)℃
Pressure Compensation Automatic, (60 to 120) kPa
Salinity Compensation Manual, (0 to 45) ppt
Range (0 to 50.0) ℃
Temperature Resolution 0.1 ℃
Accuracy ±0.5 ℃
Batteries AA x 3 (1.5V×3)
IP Rating IP57
Other Meter: 88×170×33 mm/313g
Dimensions and Weight
With case: 360×270×76 mm/1.3kg
Product Certificate RoHs, CE & ISO9001:2015

3 Instructions

3.1 LCD Screen

Fig.-1
-4-
① Measurement mode icon ⑦ Auto lock icon
② Reading/Measured Value Temperature Compensation
③ Symbol ⑧ ATC — Auto Temperature Compensation
MTC — Manual Temperature Compensation
④ Units of Measurement
⑤ Temperature unit ⑨ Reading Stability Icon
⑥ Temprature value / Symbol ⑩ Low Voltage Icon

3.2 Key Operation

Fig.-2
Short press: key press time < 2 seconds; Long press: key press time> 2 seconds.
Power on: Press to turn on. Power off: long press 2 seconds off.
Special notes: After meter power on, wait about 30 seconds to read value or operatation.

Table - 1 Key operation and functions


Key Operation Functions
Short ⚫ In the shutdown mode: press the key to boot
Press ⚫ In measurement mode: press to turn backlight on or off
Long Press ⚫ Press and hold for 2 seconds to turn off
Short ⚫ In measurement mode: press the key to switch unit: %→mg/L
Press or %→ppm
⚫ In the measurement mode: press the key for 2 seconds to enter the
Long Press
calibration mode
Short ⚫ To cancel any operation, press to return to measurement mode
Press
Short ⚫ In the menu mode: press the key to change the serial number or
Press select the parameter
Short ⚫ In the menu mode: press the key to change the serial number or
Press select the parameter
⚫ In measurement mode: press to enter menu mode;
Short
⚫ In calibration mode: press key to calibrate;
Press
⚫ In the menu mode: press key to confirm the parameter.

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3.3 Batteries
The instrument uses three AA alkaline batteries. Battery life > 200
hours (without backlight). When the display shows symbol as
shown in Figure-3, replace the battery.

3.4 Instrument Socket Fig.-3


The instrument uses an 8-pin socket that is protected by a gray rubber cap seal. Eight-pin
socket (right) - connect DO electrode. When inserting the probe connector, align the notch
on the connector to the socket, and twist the nut to tighten. There is a sealing ring between
the end face of the socket and the connector, which can effectively maintain the waterproof
rating of the socket.

3.5 Reading Stability Mode


When the measured value is stable, the LCD screen displays the icon
as shown in Figure-4. If there is no icon or icon flashing,
indicating that the reading is not stable yet, the measurement should
Fig.-4
not be read or calibrated.

3.6 Auto Lock Mode


In parameter setting P4.2 you can select the auto-lock mode (Off-On) ,
Select On to turn on automatic locking. When the reading is stable
for more than 10 seconds, the meter automatically locks the measured
value and displays the HOLD icon, as shown in Figure - 8. When auto.
locked, press to unlock. Select Off to turn off automatic lock.
Fig.-5

3.7 Backlighting
The Instruments LCD screen has a white backlight suitable for use in dark environments.
Turning on the backlight will consume more power. The instrument has automatic backlight
and manual backlight mode. In automatic backlight mode, when is pressed, backlight
will be on for a minute then automatically shuts off; In manual backlight mode, when is
pressed, backlight will be on and won’t turn off unless is pressed again. In the
parameter setting P4.3, you can select the auto backlight mode (On-Off), select On to turn
on auto. backlight; select Off to turn off auto. backlight.

3.8 Automatic Power-Off


In the parameter setting P4.4, you can select the auto power off function (On-Off), select On
to turn on auto. power off, the instrument will shut down automatically after 20 minutes’ non-
operation; select Off to disable this function.

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4 Optical Dissolved Oxygen Probe

4.1 Probe Structure


The instruments DO803 optical dissolved oxygen probe has a cable length of 3m and built-
in temperature sensor for automatic temperature compensation. The electrode structure is
shown in Fig.-6

Fig.-6

1. Bottom cover of the calibration sleeve 5. Locking cap


2. Sponge for water storage 6. Optical DO electrode
3. Sensor cap 7. O-ring

Immersion line:
4. Calibration sleeve
The tested solution should be above this line

4.2 Probe Maintenance


The sensor cap of the optical DO electrode must be kept in a moist environment. If the
surface coating of the sensor cap dries out, the reading will be unstable, or the response
rate will be slow. The electrode calibration sleeve is used to store the probe.

(A) Short-term storage (less than 30 days): The probe head is kept in the calibration
sleeve. Always keep the sponge inside the calibration sleeve moist. Several drops of
clean water should be added to a dry sponge (let the sponge be damp, but not
dripping), and tighten the lock cap, so that the sensor cap is kept in the moist-
saturated air.

(B) Long-term storage (greater than 30 days): The probe head is kept in the calibration
sleeve. Check whether the storage sponge is moist every 30 days or user can store
the electrode in a beaker containing clean water.

(C) Before the first use, unscrew the calibration sleeve to check if the sponge is damp. If
the sponge is dry or if the electrode is exposed to dry air for more than 8 hours, the
surface coating of the sensor cap may be dried out. So the electrode should be

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soaked in clean water at room temperature for 24 hours. If the water temperature is
low, soaking time should be 48-72 hours.

(D) The sponge can not be allowed to get stained or moldy, otherwise it will consume or
produce oxygen. If stained or moldy, please replace it immediately (some back-up
sponges are included in the kit).

4.3 Sensor Cap


(A) The sensor cap is a key part of the optical DO probe. The surface coating of the cap
cannot be scratched or mechanically worn. Otherwise, the service life of the sensor
cap will be reduced or the probe will be damaged. Please pay special attention to it
when using the probe.

(B) The surface coating of the sensor cap cannot withstand high temperature, so the
optical DO probe can not be tested in water above 50˚C/122˚F.

(C) If the surface of the sensor cap is contaminated, please do not use alcohol or organic
solvents to clean, otherwise it may damage the probe. It can be gently wiped with a
soft clean cloth. To disinfect the probe, immerse it in 3% hydrogen peroxide for 15 to
30 minutes and then rinse off with clean water.

(D) The sensor cap has a service life of more than 8000 hours. When the probe is not
being used, the service life will not be reduced, so the actual use time of the sensor
cap is far more than a year. The major factor affecting the service life of the sensor
cap is the surface coating being damaged under external force. So the key is to
protect the sensor cap from external damage.

(E) If the sensor cap is damaged or deteriorated, users need to purchase a new one.
Every new cap has a set of calibration codes which need to be input into the
instrument. The specific input method will be described in the instruction manual of
sensor cap.

(F) Users should not take off the sensor cap when it is not in use. Nor should one swap
the caps from different instruments. When being installed, the sensor cap must be
tightened, and the interior can not be contaminated or wet.

5 Preparation for Calibration

5.1 Dissolved Oxygen Units Selection


Dissolved oxygen units displays in two forms: mg / L and %, and ppm and %. Press to
switch between mg / L → %, or ppm → %. Users can choose mg / L or ppm in parameter
setting P3.1, but only a percentage (DO %) is displayed in calibration.

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5.2 Resolution Selection
The resolution unit can be selected in parameter setting P3.2: 0.01 or 0.1mg/L (ppm). After
setting, the meter will display resolution of 0.1 or 1 in according to %.

5.3 Temperature Unit Selection


The temperature unit can be selected in parameter setting P4.1: ˚C or ˚F

5.4 Air Pressure Compensation


The instrument has automatic air pressure compensation function. The air pressure has
been calibrated before the product left the factory. So in general users do not have to
calibrate air pressure any more. If necessary, calibrate it according to standard value
measured by aneroid barometer. Refer to parameter setting P3.4 for the procedure of
aneroid barometer calibration.

5.5 Salinity Compensation


The instrument has manual salinity compensation. It is set in the parameter P3.3 (0 to 45
ppt). To obtain accurate readings in mg/L and ppm, it is necessary to know the salinity of the
test sample and input salinity value into the instrument. As the salinity of the solution
increases, the level of DO decreases. Generally, salinity of freshwater is 0 to 0.5ppt, salinity
of seawater is 35ppt.

6 Calibration

6.1 Saturated Oxygen Calibration


(A) This procedure requires the use of a calibration sleeve to allow the probe to be calibrated
in a humidity-saturated atmosphere.
(B) Check if the sponge in the calibration sleeve is damp. Attach the calibration sleeve to
the probe. Tighten the locking cap. Be careful not to have water droplets on the head of
the sensor cap. Wait for 5 to 10 minutes after turning on the instrument in order to
saturate the air in the calibration sleeve with water vapor. In addition, wait for the
temperature to completely stablize.
(C) Long press to enter the calibration mode, and CAL is flashing in the upper right
corner. Wait for the stable to appear and stay on, press to finish calibration,
once 100% starts flashing, the saturated oxygen calibration is completed.

6.2 Zero -Oxygen Calibration


Zero-Oxygen calibration is only performed when a probe or sensor cap is replaced, the
probe has not been used for a long time, or users have a requirement for high accuracy in
low DO range (0 to 2.0 ppm). Zero-Oxygen calibration is done at the factory before shipment,
so it is not necessary to perform it during initial use. Zero-oxygen calibration should follow

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these steps:
(A) Preparation of 100ml of oxygen-free water: in the 100ml beaker, weigh 2g of
anhydrous sodium sulfite (Na2SO3) and add 100ml of distilled or deionized water to
dissolve. Oxygen-free water is only effective within 1 hour.

(B) Put the electrode into the oxygen-free water, wait for 3 to 5 minutes after the
instrument is turned on, and wait for the temperature and DO reading to completely
stabilize. The DO reading should be very close to 0, 0.1mg/L (ppm) or so.

(C) Long press to enter the calibration mode. CAL will be flashing at the upper right
corner. Wait for a stable . Press and the zero-oxygen calibration is
completed.

6.3 Special Notes for Calibration


(A) Optical dissolved oxygen probes have better stability and smaller calibration drift
compared to conventional electrochemical dissolved oxygen electrodes, which means
that the instrument can maintain its calibration data for a few months. However, for
optimal accuracy, it is recommended that the oxygen saturation calibration be performed
according to section 6.1 before use every day.
(B) Drying of the surface coating of the sensor cap can adversely affect the stability of the
measurement. Please pay special attention to this situation. See Section 4.2 (Probe
Maintenance) for details.
(C) The instrument has factory default setting function, select YES in parameter setting P3.5,
the meter will be calibrated to the theory value.

7 Measurement

7.1 When measuring, place the probe in your sample solution, stir quickly for a few
seconds in the solution to remove bubbles from the measuring surface of the sensor cap.
Then hold the probe still and wait for a stable measurement. The solution must be above
the immersion line of the probe.
Note: the brief stirring of the probe in solution is only to eliminate bubbles. Unlike
conventional galvanic/polarographic electrodes, the measuring via optical dissolved
oxygen probes does not require constant stirring of the solution or flowing fluid.

7.2 Users can read the measurements when appears and stays on. Note that the
measurement time is related to temperature. When the solution temperature and the probe
temperature is close, it takes about one minute to get the readings stablized. When the
solution temperature and the electrode temperature differ a lot, it takes about 3 minutes to
reach a stable reading. This is because the reading of dissolved oxygen is heavily

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influenced by temperature, and the probe senses temperature slower than dissolved
oxygen.

8 Parameter Settings

8.1 Press the key in the measurement mode to enter the parameter setting mode P3.0,
press the key to switch the menu P3.0 →P4.0; In P3.0 mode, press to enter
P3.1, press to switch submenu P3.1→P3.5; In P4.0 mode, press to enter P4.1,
press to switch submenu P4.1→P4.4. See Table 2 for details.

Table-2 Parameter Setting List


Menu Submenu Parameter Code Content

P3.1 DO Units Selection / mg/L—ppm

P3.2 Resolution Selection / 0.01/0.1 mg/L(ppm)


P3.0
DO P3.3 Salinity Compensation / (0 to 45) ppt
parameter
P3.4 Air Pressure Calibration (60 to 120) kPa

P3.5 Back to Factory Default No—Yes

P4.1 Temp. Unit Selection / ℃—℉

P4.0 P4.2 Auto Lock / Off—On


Basic
parameter P4.3 Auto Backlight On—Off

P4.4 Auto Power Off On—Off

8.2 DO Parameter setting (press or to switch)


P3.1—Dissolved Oxygen Unit (mg/L—ppm)
1. In P3.0 mode, press to enter P3.1 mode.
2. Press , mg / L flashes, press to select
mg/L → ppm, press to confirm.
3. Press to enter P3.2 mode, or press to return to
measurement mode.

P3.2—Resolution (0.01-0.1mg/L)
1.Press , , 0.01 flashes, press to select resolution
(0.01-0.1mg/L), press to confirm.
2. Press to enter P3.3 mode, or press to return to
measurement mode.

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P3.3—Salinity Compensation (0~45 ppt)
1. Press , 0 flashes, Press or to adjust
salinity value (0 to 45 ppt), Press to confirm.
2. Press to enter P3.4 mode, or press to return to
measurement mode.

P3.4—Air Pressure Calibration (60 to 120 kPa)


1. Press , 101.3 flashes, according to standard pressure
value. Press or to adjust, Press to confirm.
2. Press to enter P3.5 mode, or press to return to
measurement mode.

P3.5—Back to Factory Default (No—Yes)


Press , No flashes, Press to select No →Yes,
Press to confirm. Meter returns to measurement mode.
No—cancel restore. Yes—restore

8.3 Basic Parameter setting (press or to switch)

P4.1— temperature unit (℃—℉)


1. In P4.0 mode, Press to enter P4.1 mode.
2. Press , ℃ flashes, Press to select ℃→℉, Press
to confirm.
2.Press to enter P4.2 mode, or to return to
measurement mode.
P4.2—Auto Lock (Off—On)
1. Press , Off flashes, Press to select Off→On, Press
to confirm. Off—unlock; On—lock (If reading stays stable for
more than 10seconds, it auto locks).
2. Press to enter P4.3 mode, or to return to
measurement mode.

P4.3—Auto Backlight (On—Off)


1. Press , On flashes, Press to select On→Off, Press
to confirm. On - Auto backlight on, Off - Auto backlight off.
2. Press to enter P4.4 mode, or press to return to
measurement mode.

P4.4—Auto Power Off (On—Off)


1. Press , On flashe, press to select On→Off, press
to confirm.
On—auto power off on,Off—auto power off off
2. Press to return to measurement mode.

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9 Complete Kit

9.1 What’s in the box

Content Quantity
1. DO850 Portable Optical Dissolved Oxygen Meter 1
2 DO803 Optical Dissolved Oxygen Probe 1
3 Probe Calibration Sleeve 1
4 Small Screwdriver 1
5 Carrying Case 1
6 Sponge for Water Storage (spare) 4
7 Instruction Manual 1

9.2 Accessories for separate purchase

Model Name

DO803 Optical DO probe (3m cable. with sensor cap and calibration sleeve)
DO810 Optical DO probe (10m cable, with sensor cap and calibration sleeve)
DO8032 Sensor cap
DO8031 Calibration sleeve

10 Warranty

10.1 The warranty period of the DO850 instrument (only the instrument) is 3 years from the
date of purchase. That of DO803 optical DO probe (excluding sensor cap) is 2 years from
the date of purchase. That of DO8032 sensor cap is 1 year from the date of purchase. If the
above products or parts within the warranty period is not functional due to raw material
defects or poor manufacturing, they are free for repair or replace.

10.2 Damage and malfunction of the product caused by the following reasons are not
covered by the warranty:

(A) Fails to install, operate, or use the product in accordance with the instruction manual, or
if the product is damaged by abuse or incorrect use;
(B) The sensor cap is damaged by external force and can not work; or the electrode cable
is damaged or twisted due to external force;

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(C) Fails to maintain the product in accordance with the requirements of this manual and
the industry standard process;
(D) Any unauthorized repairs, and the use of defective or incorrect components to repair the
product;
(E) Any modification of the product unauthorized by the Company.

10.3 Product Warranty Period is the free of charge service time for the user who purchase
the product, not the service life of the instrument or the probe.

11 Trouble Shooting

Error Solutions
1. The battery is not installed correctly. Check the direction.
The instrument does
2. Battery low voltage, replace the battery.
not turn on
3. Instrument freezes. Take out the batteries and re-install.
1. Check calibration procedure: correct atmospheric pressure,
salinity input and temperature.
The instrument can 2. The measured value is not stable, prolong the stabilization
not calibrate time, until is fully stabilized, then press to finish.
3. Check the sensor cap. If it is contaminated, it can be cleaned; if
dried out, it can be hydrated; if damaged, it can be replaced.
1.Check whether the temperature is stable, the salinity input and
barometric pressure are accurate.
2. If the probe calibration is not good, recalibrate.
DO readings are not 3. Check the sensor cap. If it is contaminated, it can be cleaned;
accurate if dried out, it can be hydrated; if damaged it can be replaced.
4. Unscrew the sensor cap, check whether there is moisture
inside, if so, wipe off, dry, and tighten it.

1. Check whether the concentration of the sample is higher than


200% or 20.0 mg / L (ppm).
The display value
2. Check if the temperature reading is accurate.
stays 200% or 20.0 3. If the probe calibration is not good, recalibrate.
mg/L. No change 4. Check the sensor cap. If it is contaminated, it can be cleaned; if
dried out, it can be hydrated; if damaged, it can be replaced.

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Appendix A: Oxygen Solubility Table (760mm Hg)

Temp °C Chlority: 0 5.0 ppt 10.0 ppt 15.0 ppt 20.0 ppt 25.0 ppt
Salinity: 0 9.0 ppt 18.1 ppt 27.1 ppt 36.1 ppt 45.2 ppt
0.0 14.62 13.73 12.89 12.10 11.36 10.66
1.0 14.22 13.36 12.55 11.78 11.07 10.39
2.0 13.83 13.00 12.22 11.48 10.79 10.14
3.0 13.46 12.66 11.91 11.20 10.53 9.90
4.0 13.11 12.34 11.61 10.92 10.27 9.66
5.0 12.77 12.02 11.32 10.66 10.03 9.44
6.0 12.45 11.73 11.05 10.40 9.80 9.23
7.0 12.14 11.44 10.78 10.16 9.58 9.02
8.0 11.84 11.17 10.53 9.93 9.36 8.83
9.0 11.56 10.91 10.29 9.71 9.16 8.64
10.0 11.29 10.66 10.06 9.49 8.96 8.45
11.0 11.03 10.42 9.84 9.29 8.77 8.28
12.0 10.78 10.18 9.62 9.09 8.59 8.11
13.0 10.54 9.96 9.42 8.90 8.41 7.95
14.0 10.31 9.75 9.22 8.72 8.24 7.79
15.0 10.08 9.54 9.03 8.54 8.08 7.64
16.0 9.87 9.34 8.84 8.37 7.92 7.50
17.0 9.67 9.15 8.67 8.21 7.77 7.36
18.0 9.47 8.97 8.50 8.05 7.62 7.22
19.0 9.28 8.79 8.33 7.90 7.48 7.09
20.0 9.09 8.62 8.17 7.75 7.35 6.96
21.0 8.92 8.46 8.02 7.61 7.21 6.84
22.0 8.74 8.30 7.87 7.47 7.09 6.72
23.0 8.58 8.14 7.73 7.34 6.96 6.61
24.0 8.42 7.99 7.59 7.21 6.84 6.50
25.0 8.26 7.85 7.46 7.08 6.72 6.39
26.0 8.11 7.71 7.33 6.96 6.62 6.28
27.0 7.97 7.58 7.20 6.85 6.51 6.18
28.0 7.83 7.44 7.08 6.73 6.40 6.09
29.0 7.69 7.32 6.93 6.62 6.30 5.99
30.0 7.56 7.19 6.85 6.51 6.20 5.90
31.0 7.43 7.07 6.73 6.41 6.10 5.81
32.0 7.31 6.96 6.62 6.31 6.01 5.72
33.0 7.18 6.84 6.52 6.21 5.91 5.63
34.0 7.07 6.73 6.42 6.11 5.82 5.55
35.0 6.95 6.62 6.31 6.02 5.73 5.46
36.0 6.84 6.52 6.22 5.93 5.65 5.38
37.0 6.73 6.42 6.12 5.84 5.56 5.31
38.0 6.62 6.32 6.03 5.75 5.48 5.23
39.0 6.52 6.22 5.98 5.66 5.40 5.15
40.0 6.41 6.12 5.84 5.58 5.32 5.08
41.0 6.31 6.03 5.75 5.49 5.24 5.01
42.0 6.21 5.93 5.67 5.41 5.17 4.93
43.0 6.12 5.84 5.58 5.33 5.09 4.86
44.0 6.02 5.75 5.50 5.25 5.02 4.79
45.0 5.93 5.67 5.41 5.17 4.94 4.72
Salinity = Dissolved salts in water.
Chlorinity = Measure of chloride content, by mass, of water.
S (‰) = 1.80655 x Chlorinity (‰)

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Appendix B: DO % Calibration Values

Cal. Cal.
Pressure Pressure
value value

D.O. % in Hg mmHg kPa mbar D.O. % in Hg mmHg kPa mbar


101% 30.22 767.6 102.34 1023.38 86% 25.73 653.6 87.14 871.40

100% 29.92 760.0 101.33 1013.25 85% 25.43 646.0 86.13 861.26

99% 29.62 752.4 100.31 1003.12 84% 25.13 638.4 85.11 851.13

98% 29.32 744.8 99.30 992.99 83% 24.83 630.8 84.10 841.00

97% 29.02 737.2 98.29 982.85 82% 24.54 623.2 83.09 830.87

96% 28.72 729.6 97.27 972.72 81% 24.24 615.6 82.07 820.73

95% 28.43 722.0 96.26 962.59 80% 23.94 608.0 81.06 810.60

94% 28.13 714.4 95.25 952.46 79% 23.64 600.4 80.05 800.47

93% 27.83 706.8 94.23 942.32 78% 23.34 592.8 79.03 790.34

92% 27.53 699.2 93.22 932.19 77% 23.04 585.2 78.02 780.20

91% 27.23 691.6 92.21 922.06 76% 22.74 577.6 77.01 770.07

90% 26.93 684.0 91.19 911.93 75% 22.44 570.0 75.99 759.94

89% 26.63 676.4 90.18 901.79 74% 22.14 562.4 74.98 749.81

88% 26.33 668.8 89.17 891.66 73% 21.84 554.8 73.97 739.67

87% 26.03 661.2 88.15 881.53 72% 21.54 547.2 72.95 729.54

APERA INSTRUMENTS, LLC


Address: 6656 Busch Blvd, Columbus Ohio 43229
Tel: 1-614-285-3080
Email: info@aperainst.com
Website: https://aperainst.com

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