Portable Optical Dissolved Oxygen Meter Instruction Manual: Apera Instruments, LLC
Portable Optical Dissolved Oxygen Meter Instruction Manual: Apera Instruments, LLC
Portable Optical Dissolved Oxygen Meter Instruction Manual: Apera Instruments, LLC
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Contents
1 Overview .................................................................................................................. - 3 -
1.1 Luminescent optical sensor. ........................................................................................................... - 3 -
1.2 Intelligent Instrumentation ............................................................................................................ - 3 -
2 Technical Specifications ........................................................................................... - 4 -
3 Instructions ............................................................................................................... - 4 -
3.1 LCD Screen ...................................................................................................................................... - 4 -
3.2 Key Operation ................................................................................................................................. - 5 -
3.3 Batteries ......................................................................................................................................... - 6 -
3.4 Instrument Socket .......................................................................................................................... - 6 -
3.5 Reading Stability Mode .................................................................................................................. - 6 -
3.6 Auto Lock Mode ............................................................................................................................. - 6 -
3.7 Backlighting .................................................................................................................................... - 6 -
3.8 Automatic Power-Off ...................................................................................................................... - 6 -
4 Optical Dissolved Oxygen Probe .............................................................................. - 7 -
4.1 Probe Structure .............................................................................................................................. - 7 -
4.2 Probe Maintenance ........................................................................................................................ - 7 -
4.3 Sensor Cap ...................................................................................................................................... - 8 -
5 Preparation for Calibration ........................................................................................ - 8 -
5.1 Dissolved Oxygen Units Selection .................................................................................................. - 8 -
5.3 Temperature Unit Selection............................................................................................................. - 9 -
5.4 Air Pressure Compensation ............................................................................................................. - 9 -
6 Calibration ................................................................................................................ - 9 -
6.1 Saturated Oxygen Calibration ......................................................................................................... - 9 -
6.2 Zero -Oxygen Calibration ................................................................................................................ - 9 -
6.3 Special Notes for Calibration ........................................................................................................ - 10 -
7 Measurement.......................................................................................................... - 10 -
8 Parameter Settings .................................................................................................. - 11 -
9 Complete Kit............................................................................................................ - 13 -
10 Warranty ................................................................................................................ - 13 -
11 Trouble Shooting ................................................................................................... - 14 -
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1 Overview
Thank you for purchasing Apera Instruments DO850 Portable Optical Dissolved Oxygen Meter. The
DO850 measures dissolved oxygen in water using luminescence technology through an optical
sensor and displays data with intelligent instrumentation. Compared to conventional electrochemical
dissolved oxygen meter, the DO850 is more accurate, stable and easier to use.
Before you use the instrument, please carefully read the instruction manual to help you properly
perform tests and maintainenance.
Special Notes
⚫ Sensor cap surface coating can not withstand high temperature, so the optical dissolved
oxygen electrode can not test water with temperature over 50 ℃.
⚫ When the electrode is not in use, it should be kept in the storage sleeve and the
sponge in the cap should be kept moist, so that the fluorescent cap will not dry out. If
the sponge is dried out or the probe is exposed in dry air for more than 8 hours, soak
the electrode in tap water for 24~48 hours (see section 4.2), otherwise it may cause
unstable measurements or slow response.
⚫ Before getting readings or performing other operations, wait about 30 seconds after meter is
powered on.
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2 Technical Specifications
3 Instructions
Fig.-1
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① Measurement mode icon ⑦ Auto lock icon
② Reading/Measured Value Temperature Compensation
③ Symbol ⑧ ATC — Auto Temperature Compensation
MTC — Manual Temperature Compensation
④ Units of Measurement
⑤ Temperature unit ⑨ Reading Stability Icon
⑥ Temprature value / Symbol ⑩ Low Voltage Icon
Fig.-2
Short press: key press time < 2 seconds; Long press: key press time> 2 seconds.
Power on: Press to turn on. Power off: long press 2 seconds off.
Special notes: After meter power on, wait about 30 seconds to read value or operatation.
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3.3 Batteries
The instrument uses three AA alkaline batteries. Battery life > 200
hours (without backlight). When the display shows symbol as
shown in Figure-3, replace the battery.
3.7 Backlighting
The Instruments LCD screen has a white backlight suitable for use in dark environments.
Turning on the backlight will consume more power. The instrument has automatic backlight
and manual backlight mode. In automatic backlight mode, when is pressed, backlight
will be on for a minute then automatically shuts off; In manual backlight mode, when is
pressed, backlight will be on and won’t turn off unless is pressed again. In the
parameter setting P4.3, you can select the auto backlight mode (On-Off), select On to turn
on auto. backlight; select Off to turn off auto. backlight.
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4 Optical Dissolved Oxygen Probe
Fig.-6
Immersion line:
4. Calibration sleeve
The tested solution should be above this line
(A) Short-term storage (less than 30 days): The probe head is kept in the calibration
sleeve. Always keep the sponge inside the calibration sleeve moist. Several drops of
clean water should be added to a dry sponge (let the sponge be damp, but not
dripping), and tighten the lock cap, so that the sensor cap is kept in the moist-
saturated air.
(B) Long-term storage (greater than 30 days): The probe head is kept in the calibration
sleeve. Check whether the storage sponge is moist every 30 days or user can store
the electrode in a beaker containing clean water.
(C) Before the first use, unscrew the calibration sleeve to check if the sponge is damp. If
the sponge is dry or if the electrode is exposed to dry air for more than 8 hours, the
surface coating of the sensor cap may be dried out. So the electrode should be
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soaked in clean water at room temperature for 24 hours. If the water temperature is
low, soaking time should be 48-72 hours.
(D) The sponge can not be allowed to get stained or moldy, otherwise it will consume or
produce oxygen. If stained or moldy, please replace it immediately (some back-up
sponges are included in the kit).
(B) The surface coating of the sensor cap cannot withstand high temperature, so the
optical DO probe can not be tested in water above 50˚C/122˚F.
(C) If the surface of the sensor cap is contaminated, please do not use alcohol or organic
solvents to clean, otherwise it may damage the probe. It can be gently wiped with a
soft clean cloth. To disinfect the probe, immerse it in 3% hydrogen peroxide for 15 to
30 minutes and then rinse off with clean water.
(D) The sensor cap has a service life of more than 8000 hours. When the probe is not
being used, the service life will not be reduced, so the actual use time of the sensor
cap is far more than a year. The major factor affecting the service life of the sensor
cap is the surface coating being damaged under external force. So the key is to
protect the sensor cap from external damage.
(E) If the sensor cap is damaged or deteriorated, users need to purchase a new one.
Every new cap has a set of calibration codes which need to be input into the
instrument. The specific input method will be described in the instruction manual of
sensor cap.
(F) Users should not take off the sensor cap when it is not in use. Nor should one swap
the caps from different instruments. When being installed, the sensor cap must be
tightened, and the interior can not be contaminated or wet.
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5.2 Resolution Selection
The resolution unit can be selected in parameter setting P3.2: 0.01 or 0.1mg/L (ppm). After
setting, the meter will display resolution of 0.1 or 1 in according to %.
6 Calibration
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these steps:
(A) Preparation of 100ml of oxygen-free water: in the 100ml beaker, weigh 2g of
anhydrous sodium sulfite (Na2SO3) and add 100ml of distilled or deionized water to
dissolve. Oxygen-free water is only effective within 1 hour.
(B) Put the electrode into the oxygen-free water, wait for 3 to 5 minutes after the
instrument is turned on, and wait for the temperature and DO reading to completely
stabilize. The DO reading should be very close to 0, 0.1mg/L (ppm) or so.
(C) Long press to enter the calibration mode. CAL will be flashing at the upper right
corner. Wait for a stable . Press and the zero-oxygen calibration is
completed.
7 Measurement
7.1 When measuring, place the probe in your sample solution, stir quickly for a few
seconds in the solution to remove bubbles from the measuring surface of the sensor cap.
Then hold the probe still and wait for a stable measurement. The solution must be above
the immersion line of the probe.
Note: the brief stirring of the probe in solution is only to eliminate bubbles. Unlike
conventional galvanic/polarographic electrodes, the measuring via optical dissolved
oxygen probes does not require constant stirring of the solution or flowing fluid.
7.2 Users can read the measurements when appears and stays on. Note that the
measurement time is related to temperature. When the solution temperature and the probe
temperature is close, it takes about one minute to get the readings stablized. When the
solution temperature and the electrode temperature differ a lot, it takes about 3 minutes to
reach a stable reading. This is because the reading of dissolved oxygen is heavily
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influenced by temperature, and the probe senses temperature slower than dissolved
oxygen.
8 Parameter Settings
8.1 Press the key in the measurement mode to enter the parameter setting mode P3.0,
press the key to switch the menu P3.0 →P4.0; In P3.0 mode, press to enter
P3.1, press to switch submenu P3.1→P3.5; In P4.0 mode, press to enter P4.1,
press to switch submenu P4.1→P4.4. See Table 2 for details.
P3.2—Resolution (0.01-0.1mg/L)
1.Press , , 0.01 flashes, press to select resolution
(0.01-0.1mg/L), press to confirm.
2. Press to enter P3.3 mode, or press to return to
measurement mode.
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P3.3—Salinity Compensation (0~45 ppt)
1. Press , 0 flashes, Press or to adjust
salinity value (0 to 45 ppt), Press to confirm.
2. Press to enter P3.4 mode, or press to return to
measurement mode.
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9 Complete Kit
Content Quantity
1. DO850 Portable Optical Dissolved Oxygen Meter 1
2 DO803 Optical Dissolved Oxygen Probe 1
3 Probe Calibration Sleeve 1
4 Small Screwdriver 1
5 Carrying Case 1
6 Sponge for Water Storage (spare) 4
7 Instruction Manual 1
Model Name
DO803 Optical DO probe (3m cable. with sensor cap and calibration sleeve)
DO810 Optical DO probe (10m cable, with sensor cap and calibration sleeve)
DO8032 Sensor cap
DO8031 Calibration sleeve
10 Warranty
10.1 The warranty period of the DO850 instrument (only the instrument) is 3 years from the
date of purchase. That of DO803 optical DO probe (excluding sensor cap) is 2 years from
the date of purchase. That of DO8032 sensor cap is 1 year from the date of purchase. If the
above products or parts within the warranty period is not functional due to raw material
defects or poor manufacturing, they are free for repair or replace.
10.2 Damage and malfunction of the product caused by the following reasons are not
covered by the warranty:
(A) Fails to install, operate, or use the product in accordance with the instruction manual, or
if the product is damaged by abuse or incorrect use;
(B) The sensor cap is damaged by external force and can not work; or the electrode cable
is damaged or twisted due to external force;
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(C) Fails to maintain the product in accordance with the requirements of this manual and
the industry standard process;
(D) Any unauthorized repairs, and the use of defective or incorrect components to repair the
product;
(E) Any modification of the product unauthorized by the Company.
10.3 Product Warranty Period is the free of charge service time for the user who purchase
the product, not the service life of the instrument or the probe.
11 Trouble Shooting
Error Solutions
1. The battery is not installed correctly. Check the direction.
The instrument does
2. Battery low voltage, replace the battery.
not turn on
3. Instrument freezes. Take out the batteries and re-install.
1. Check calibration procedure: correct atmospheric pressure,
salinity input and temperature.
The instrument can 2. The measured value is not stable, prolong the stabilization
not calibrate time, until is fully stabilized, then press to finish.
3. Check the sensor cap. If it is contaminated, it can be cleaned; if
dried out, it can be hydrated; if damaged, it can be replaced.
1.Check whether the temperature is stable, the salinity input and
barometric pressure are accurate.
2. If the probe calibration is not good, recalibrate.
DO readings are not 3. Check the sensor cap. If it is contaminated, it can be cleaned;
accurate if dried out, it can be hydrated; if damaged it can be replaced.
4. Unscrew the sensor cap, check whether there is moisture
inside, if so, wipe off, dry, and tighten it.
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Appendix A: Oxygen Solubility Table (760mm Hg)
Temp °C Chlority: 0 5.0 ppt 10.0 ppt 15.0 ppt 20.0 ppt 25.0 ppt
Salinity: 0 9.0 ppt 18.1 ppt 27.1 ppt 36.1 ppt 45.2 ppt
0.0 14.62 13.73 12.89 12.10 11.36 10.66
1.0 14.22 13.36 12.55 11.78 11.07 10.39
2.0 13.83 13.00 12.22 11.48 10.79 10.14
3.0 13.46 12.66 11.91 11.20 10.53 9.90
4.0 13.11 12.34 11.61 10.92 10.27 9.66
5.0 12.77 12.02 11.32 10.66 10.03 9.44
6.0 12.45 11.73 11.05 10.40 9.80 9.23
7.0 12.14 11.44 10.78 10.16 9.58 9.02
8.0 11.84 11.17 10.53 9.93 9.36 8.83
9.0 11.56 10.91 10.29 9.71 9.16 8.64
10.0 11.29 10.66 10.06 9.49 8.96 8.45
11.0 11.03 10.42 9.84 9.29 8.77 8.28
12.0 10.78 10.18 9.62 9.09 8.59 8.11
13.0 10.54 9.96 9.42 8.90 8.41 7.95
14.0 10.31 9.75 9.22 8.72 8.24 7.79
15.0 10.08 9.54 9.03 8.54 8.08 7.64
16.0 9.87 9.34 8.84 8.37 7.92 7.50
17.0 9.67 9.15 8.67 8.21 7.77 7.36
18.0 9.47 8.97 8.50 8.05 7.62 7.22
19.0 9.28 8.79 8.33 7.90 7.48 7.09
20.0 9.09 8.62 8.17 7.75 7.35 6.96
21.0 8.92 8.46 8.02 7.61 7.21 6.84
22.0 8.74 8.30 7.87 7.47 7.09 6.72
23.0 8.58 8.14 7.73 7.34 6.96 6.61
24.0 8.42 7.99 7.59 7.21 6.84 6.50
25.0 8.26 7.85 7.46 7.08 6.72 6.39
26.0 8.11 7.71 7.33 6.96 6.62 6.28
27.0 7.97 7.58 7.20 6.85 6.51 6.18
28.0 7.83 7.44 7.08 6.73 6.40 6.09
29.0 7.69 7.32 6.93 6.62 6.30 5.99
30.0 7.56 7.19 6.85 6.51 6.20 5.90
31.0 7.43 7.07 6.73 6.41 6.10 5.81
32.0 7.31 6.96 6.62 6.31 6.01 5.72
33.0 7.18 6.84 6.52 6.21 5.91 5.63
34.0 7.07 6.73 6.42 6.11 5.82 5.55
35.0 6.95 6.62 6.31 6.02 5.73 5.46
36.0 6.84 6.52 6.22 5.93 5.65 5.38
37.0 6.73 6.42 6.12 5.84 5.56 5.31
38.0 6.62 6.32 6.03 5.75 5.48 5.23
39.0 6.52 6.22 5.98 5.66 5.40 5.15
40.0 6.41 6.12 5.84 5.58 5.32 5.08
41.0 6.31 6.03 5.75 5.49 5.24 5.01
42.0 6.21 5.93 5.67 5.41 5.17 4.93
43.0 6.12 5.84 5.58 5.33 5.09 4.86
44.0 6.02 5.75 5.50 5.25 5.02 4.79
45.0 5.93 5.67 5.41 5.17 4.94 4.72
Salinity = Dissolved salts in water.
Chlorinity = Measure of chloride content, by mass, of water.
S (‰) = 1.80655 x Chlorinity (‰)
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Appendix B: DO % Calibration Values
Cal. Cal.
Pressure Pressure
value value
100% 29.92 760.0 101.33 1013.25 85% 25.43 646.0 86.13 861.26
99% 29.62 752.4 100.31 1003.12 84% 25.13 638.4 85.11 851.13
98% 29.32 744.8 99.30 992.99 83% 24.83 630.8 84.10 841.00
97% 29.02 737.2 98.29 982.85 82% 24.54 623.2 83.09 830.87
96% 28.72 729.6 97.27 972.72 81% 24.24 615.6 82.07 820.73
95% 28.43 722.0 96.26 962.59 80% 23.94 608.0 81.06 810.60
94% 28.13 714.4 95.25 952.46 79% 23.64 600.4 80.05 800.47
93% 27.83 706.8 94.23 942.32 78% 23.34 592.8 79.03 790.34
92% 27.53 699.2 93.22 932.19 77% 23.04 585.2 78.02 780.20
91% 27.23 691.6 92.21 922.06 76% 22.74 577.6 77.01 770.07
90% 26.93 684.0 91.19 911.93 75% 22.44 570.0 75.99 759.94
89% 26.63 676.4 90.18 901.79 74% 22.14 562.4 74.98 749.81
88% 26.33 668.8 89.17 891.66 73% 21.84 554.8 73.97 739.67
87% 26.03 661.2 88.15 881.53 72% 21.54 547.2 72.95 729.54
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