Urinepreservatives Preservatives Advantages Disadvantages Additional Information

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URINE PRESERV ATIVES

Preservatives Advantages Disadvantages Additional Information


Refrigeration - Does not interfere with chemical - Raises specific gravity by - Prevents bacterial growth 24 h
tests hydrometer
- Precipitates amorphous
phosphates and urates
Thymol - Preserves glucose and sediments - Interferes with acid precipitation
well tests for protein
Boric acid - Preserves protein and formed - May precipitate crystals when used - Keeps pH at about 6.0
elements well in large amounts
- Does not interfere with routine - Is bacteriostatic (not bactericidal) at
analyses other than pH 18 g/L; can use for culture transport
- Interferes with drug and hormone
analyses
Formalin (formaldehyde) - Excellent sediment preservative - Acts as a reducing agent, - Rinse specimen container with
interfering with chemical tests for formalin to preserve cells and casts
glucose, blood, leukocyte esterase,
and copper reduction
Toluene - Does not interfere with routine tests - Floats on surface of specimens and
clings to pipettes and testing
materials
Sodium fluoride - Prevents glycolysis - Inhibits reagent strip tests for - May use sodium benzoate instead
- Is a good preservative for drug glucose, blood, and leukocytes of fluoride for reagent strip testing
analyses
Phenol - Does not interfere with routine tests - Causes an odor change - Use 1 drop per ounce of specimen
Commercial preservative - Convenient when refrigeration not - May contain one or more of the - Check tablet composition to
tablets possible preservatives including sodium determine possible effects on
- Have controlled concentration to fluoride desired tests
minimize interference
Urine Collection Kits (Becton - Contains collection cup, C&S
Dickinson, Rutherford, NJ) preservative tube or UA tube
Gray C&S tube - Sample stable at room temperature - Decreases pH; do not use if urine is - Preservative is boric acid and may
(RT) for 48 hr; preserves bacteria below minimum fill line not be used for UA
Yellow plain UA tube - Use on automated instruments - Must refrigerate within 2 hours - Round or conical bottom
Cherry red/yellow top tube - Stable for 72 hours at RT; - Bilirubin and urobilinogen may be - Preservative is sodium propionate;
instrument-compatible decreased if specimen is exposed to conical bottom
light and left at RT
Saccomanno Fixative - Preserves cellular elements - Used for cytology studies

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