May 2008

EZ1® Advanced User Manual

9001410, 9001411

1050261

QIAGEN GmbH, D-40724 Hilden

R2

Sample & Assay Technologies

Trademarks: QIAGEN®, EZ1® (QIAGEN Group); DECON-QUAT® (Veltek Associates, Inc.); Gigasept®, Lysetol®, Mikrozid® (Schülke & Mayr GmbH);
IBM® (International Business Machines Corporation); Microsoft®, Windows® (Microsoft Corporation).

© 2008 QIAGEN, all rights reserved.

 Contents

Contents
1 Safety Information 1-1
1.1 Proper use 1-2
1.2 Electrical safety 1-3
1.3 Environment 1-4
1.4 Biological safety 1-4
1.5 Chemicals 1-6
1.6 Waste disposal 1-6
1.7 Mechanical hazards 1-7
1.8 Heat hazard 1-7
1.9 UV radiation 1-7
1.10 Symbols on the EZ1 Advanced 1-8
1.11 Additional symbols in this user manual 1-9

2 Introduction 2-1
2.1 About this user manual 2-1
2.2 General information 2-1
2.2.1 Technical assistance 2-1
2.2.2 Policy statement 2-2
2.2.3 Version management 2-2
2.3 Intended use of the EZ1 Advanced 2-2
2.3.1 Requirements for EZ1 Advanced users 2-3

3 General Description 3-1

3.1 External features of the EZ1 Advanced 3-2
3.1.1 EZ1 Advanced door 3-2
3.1.2 Control panel 3-2
3.1.3 EZ1 Advanced Card slot 3-4
3.1.4 Status LEDs 3-5

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Contents

3.1.5 Connector panel 3-5

3.2 Internal features of the EZ1 Advanced 3-6
3.2.1 Worktable 3-6
3.2.2 Pipettor head 3-11
3.2.3 UV lamp 3-12

4 Installation Procedures 4-1

4.1 Requirements 4-1
4.2 AC power connection 4-1
4.3 Hardware installation 4-3
4.3.1 Unpacking the EZ1 Advanced 4-4
4.3.2 Installing the EZ1 Advanced 4-8
4.3.3 Configuring and setting up the EZ1 Advanced 4-9
4.3.4 Installing the printer (optional) 4-10
4.3.5 Installing the bar code reader 4-10
4.4 Installing the PC software 4-11
4.4.1 System requirements 4-12
4.4.2 Installing the driver of the USB-RS232 converter 4-12
4.4.3 Installing the EZ1 Advanced Communicator
software 4-16
4.5 Installing multiple EZ1 Advanced instruments 4-17
4.6 Transportation and removal of the EZ1 Advanced 4-19

5 General Operation 5-1

5.1 Overview 5-1
5.2 Inserting and removing the EZ1 Advanced Card 5-2
5.2.1 Inserting the EZ1 Advanced Card 5-2
5.2.2 Removing the EZ1 Advanced Card 5-4
5.3 Switching the EZ1 Advanced on and off 5-5
5.3.1 Switching on the EZ1 Advanced 5-5
5.3.2 Switching off the EZ1 Advanced 5-5
5.4 Opening and closing the EZ1 Advanced door 5-5

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 Contents

5.4.1 Opening the EZ1 Advanced door 5-5

5.4.2 Closing the EZ1 Advanced door 5-6
5.5 Starting and stopping a protocol run 5-7
5.5.1 Starting and finishing a protocol run 5-7
5.5.2 Stopping a protocol run 5-8
5.6 Setting up the worktable 5-9
5.6.1 Removing and replacing the tray 5-9
5.6.2 Loading the reagent cartridges 5-10
5.6.3 Loading the elution tubes, filter-tips, and
sample tubes 5-11
5.6.4 External tube rack 5-13
5.7 Using the UV lamp 5-13
5.7.1 Switching on the UV lamp 5-14
5.7.2 Switching off the UV lamp 5-15
5.7.3 Lifetime reminder of the UV lamp 5-16
5.7.4 UV lamp ignition error 5-16
5.8 Manual operation 5-17
5.8.1 Manual operation “home axis” 5-17
5.8.2 Manual operation “return tip” 5-18
5.8.3 Manual operation “clean” 5-18
5.8.4 Manual operation “resend” 5-19
5.9 Test 5-20
5.9.1 Test axis 5-21
5.9.2 Test heating block 5-22
5.9.3 Test serial port 5-23
5.9.4 Test version 5-26
5.10 System setup 5-26
5.10.1 Setting the date 5-26
5.10.2 Setting the time 5-27
5.10.3 Setting the serial port 5-28
5.10.4 Setting the preventive maintenance reminder 5-29
5.11 Using the bar code reader 5-29
5.12 Using the external printer (optional) 5-30

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Contents

5.13 Generating a report file 5-32

5.14 Using the EZ1 Advanced Communicator software 5-36
5.14.1 User interface 5-36
5.15 Using multiple EZ1 Advanced instruments 5-40
5.16 Evaluating pipetting accuracy 5-40

6 Preventive Maintenance 6-1

6.1 Regular maintenance procedure 6-3
6.2 Daily maintenance procedure 6-6
6.3 Weekly maintenance procedure 6-7
6.4 Reagents for decontamination 6-8

7 Troubleshooting 7-1
7.1 Detecting an error 7-1
7.1.1 EZ1 Advanced door errors 7-1
7.1.2 Other errors 7-1
7.2 Error codes 7-3

8 Glossary 8-1

Appendix A A-1
Technical data A-1
Environmental conditions A-1
Waste Electrical and Electronic Equipment (WEEE) A-6
EC declaration of conformity A-7

Appendix B B-1
Liability clause B-1

Index Index-1

Contents-4 EZ1 Advanced User Manual 05/2008

 Safety Information

1 Safety Information
This manual contains information and warnings that must be
followed by the user to ensure safe operation of the EZ1
Advanced and to maintain the instrument in a safe condition.
Possible hazards that could harm the user or result in
damage to the instrument are clearly stated at the
appropriate places throughout this manual.
If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may
be impaired.
The following safety conventions are used throughout this
manual.
WARNING The term WARNING is used to inform you about
situations that could result in personal injury to you or
other persons.
Details about these circumstances are given in a box like
this one.

CAUTION The term CAUTION is used to inform you about

situations that could result in damage to the
instrument or other equipment.
Details about these circumstances are given in a box like
this one.

Before using the instrument, it is essential to read this

manual carefully and to pay particular attention to any
advice it contains concerning hazards that may arise from
use of the instrument.
The advice given in this manual is intended to supplement,
not supersede, the normal safety requirements prevailing in
the user’s country.

EZ1 Advanced User Manual 05/2008 1-1

Safety Information

1.1 Proper use

WARNING Risk of personal injury and material damage [W1]
Improper use of the EZ1 Advanced may cause personal
injuries or damage to the instrument.
The EZ1 Advanced must only be operated by qualified
personnel who have been appropriately trained.
Servicing of the EZ1 Advanced must only be performed
by QIAGEN Instrument Service Specialists.

Use only QIAGEN® components, otherwise your right to

make a claim under the guarantee may be invalidated.
Carry out the maintenance regularly in accordance with the
operating instructions. QIAGEN charges for repairs that
prove to be required due to incorrect maintenance.

WARNING Risk of personal injury [W2]

Two people must carry the instrument. Use the handle
attached to the box to lift the EZ1 Advanced. After the
EZ1 Advanced has been unpacked, 2 people must lift
the instrument. Lift the instrument by placing your hands
underneath the bottom of the instrument.

In case of an emergency or malfunction, switch off the EZ1

Advanced at the power switch at the rear of the instrument
and unplug the power cord from the wall power outlet.
Contact the address stated on the back cover of the EZ1
Advanced User Manual.

WARNING Explosive atmosphere [W3]

The EZ1 Advanced is not designed for use in an
explosive atmosphere. The EZ1 Advanced is intended for
use with reagents and substances supplied with QIAGEN
kits. Use of other reagents and substances described in
this manual may lead to fire or explosion.

1-2 EZ1 Advanced User Manual 05/2008

 Safety Information

CAUTION Damage to the instrument [C1]

Avoid spilling water or chemicals onto the EZ1
Advanced. Instrument damage caused by water or
chemical spillage will void your warranty.

CAUTION Damage to the instrument [C2]

Ensure that the EZ1 Advanced is switched off before you
manually move the mechanical components of the
instrument.

1.2 Electrical safety

To ensure satisfactory and safe operation of the EZ1
Advanced, it is essential that the line power cord is connected
to true electrical earth (ground).
WARNING Electrical hazard [W4]
Any interruption of the protective conductor
(earth/ground lead) inside or outside the instrument or
disconnection of the protective conductor terminal is
likely to make the instrument dangerous.
Intentional interruption is prohibited.
Lethal voltages inside the instrument
When the instrument is connected to line power,
terminals may be live, and opening covers or removing
parts is likely to expose live parts.

When working with the EZ1 Advanced:

„ Make sure the line power cord is connected to a line
power outlet that has a protective conductor (earth/
ground).
„ Do not attempt to make any internal adjustments or
replacements.
„ Do not operate the instrument with any covers or parts
removed.
„ If water or reagent has spilled inside the instrument,
switch off the instrument and disconnect it from the line
power supply. Call QIAGEN Technical Services.
„ Servicing should be carried out only by QIAGEN
Instrument Service Specialists.

EZ1 Advanced User Manual 05/2008 1-3

Safety Information

„ If the instrument becomes electrically unsafe for use,

make the instrument inoperative and secure it against
unauthorized or unintentional operation. Call QIAGEN
Technical Services.
The instrument is likely to be electrically unsafe when:
„ It shows visible damage
„ The line power cord shows signs of damage
„ It has been stored under unfavorable conditions for a
prolonged period
„ It has been subjected to severe transport stresses
If water, reagent, sample, or ethanol has spilled onto or into
the instrument, switch off the instrument and disconnect it
from the line power supply. Call QIAGEN Technical Services.

WARNING Risk of electric shock [W5]

Do not touch the EZ1 Advanced with wet hands.

1.3 Environment
Operating conditions

WARNING Explosive atmosphere [W6]

The EZ1 Advanced is not designed for use in an
explosive atmosphere.

1.4 Biological safety

Specimens and reagents containing materials from humans
should be treated as potentially infectious. Use safe
laboratory procedures as outlined in publications such as
Biosafety in Microbiological and Biomedical Laboratories,
HHS (www.cdc.gov/od/ohs/biosfty/biosfty.htm).

1-4 EZ1 Advanced User Manual 05/2008

 Safety Information

Samples
Samples may contain infectious agents. You should be aware
of the health hazard presented by such agents and should
use, store, and dispose of such samples in accordance with
the required safety regulations.

WARNING Samples containing infectious agents [W7]

Some samples used with this instrument may contain
infectious agents. Handle such samples with the greatest
of care and in accordance with the required safety
regulations.
Always wear safety glasses, 2 pairs of gloves, and a lab
coat.
The responsible body (e.g., laboratory manager) must
take the necessary precautions to ensure that the
surrounding workplace is safe, and that the instrument
operators are suitably trained and not exposed to
hazardous levels of infectious agents as defined in the
applicable Material Safety Data Sheets (MSDSs) or
OSHA, ACGIH, or COSHH documents.
Venting for fumes and disposal of wastes must be in
accordance with all national, state, and local health and
safety regulations and laws.
OSHA: Occupational Safety and Health Administration (United States of America).
ACGIH: American Conference of Government Industrial Hygienists (United States of America).
COSHH: Control of Substances Hazardous to Health (United Kingdom).

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Safety Information

1.5 Chemicals
WARNING Hazardous chemicals [W8]
Some chemicals used with this instrument may be
hazardous or may become hazardous after completion
of the protocol run.
Always wear safety glasses, gloves, and a lab coat.
The responsible body (e.g., laboratory manager) must
take the necessary precautions to ensure that the
surrounding workplace is safe and that the instrument
operators are not exposed to hazardous levels of toxic
substances (chemical or biological) as defined in the
applicable Material Safety Data Sheets (MSDSs) or
OSHA, ACGIH, or COSHH documents.
Venting for fumes and disposal of wastes must be in
accordance with all national, state, and local health and
safety regulations and laws.
OSHA: Occupational Safety and Health Administration (United States of America).
ACGIH: American Conference of Government Industrial Hygienists (United States of America).
COSHH: Control of Substances Hazardous to Health (United Kingdom).

Toxic fumes
If you work with volatile solvents, toxic substances, etc., you
must provide an efficient laboratory ventilation system to
remove vapors that may be produced.

1.6 Waste disposal

Used labware, such as reagent cartridges and filter-tips, may
contain hazardous chemicals or infectious agents from the
purification process. Such wastes must be collected and
disposed of properly in accordance with the local safety
regulations.
For disposal of waste electrical and electronic equipment
(WEEE), see page A-6.

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 Safety Information

1.7 Mechanical hazards

The worktable of the EZ1 Advanced moves during operation
of the instrument.
WARNING Moving parts [W9]
Never open the EZ1 Advanced door while the EZ1
Advanced is operating.

WARNING Risk of overheating [W10]

To ensure proper ventilation, maintain a minimum
clearance of 15 cm and 30 cm at the rear and sides of
the EZ1 Advanced, respectively.
Slits and openings that ensure the ventilation of the EZ1
Advanced must not be covered.

1.8 Heat hazard

The EZ1 Advanced worktable contains a heating system.
WARNING Hot surface [W11]
The heating system can reach high temperatures. Avoid
touching it when it is hot.

1.9 UV radiation
WARNING The EZ1 Advanced contains a UV lamp [W12]
Avoid looking directly into UV light. Do not expose your
skin to UV light.

The wavelength of the UV light produced by the UV lamp is

253.7 nm. This wavelength corresponds to ultraviolet light
type C, which can be used for decontamination procedures.
The UV lamp does not produce any ozone.

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Safety Information

1.10 Symbols on the EZ1 Advanced

Symbol Location Description
Heating Heat hazard — the temperature of the
system heating system can reach up to 95ºC

Near the Biohazard — the tip rack may be

tip rack contaminated with biohazardous material
and must be handled with gloves

On the UV radiation hazard. UV light could burn

back of the your skin and cause damage to your eye.
instrument

Near the Crush hazard — the nozzle unit may crush

pipettor your fingers or hand
head

Near the UV lamp heat hazard — the UV lamp is hot.

tip rack Do not touch the UV lamp

Type plate CE mark for Europe

on the
back of the
instrument
Type plate UL listing mark for Canada and the USA
on the
back of the
instrument

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 Safety Information

Symbol Location Description

Type plate RoHS mark for China (the restriction of the
on the use of certain hazardous substances in
back of the electrical and electronic equipment)
instrument
Type plate WEEE mark for Europe
on the
back of the
instrument

SN Type plate Serial number

on the
back of the
instrument
Type plate Legal manufacturer
on the
back of the
instrument

1.11 Additional symbols in this user manual

Symbol Location Description
Front Catalog number
cover

Front Handbook
cover

EZ1 Advanced User Manual 05/2008 1-9

Safety Information

Symbol on the heating system.

Symbol near the tip rack.

Symbol on back of the EZ1 Advanced.

1-10 EZ1 Advanced User Manual 05/2008

 Introduction

2 Introduction
Thank you for choosing the QIAGEN EZ1 Advanced. We are
confident it will become an integral part of your laboratory.
Before using the instrument, it is essential to read this
manual carefully and to pay particular attention to any
advice it contains concerning hazards that may arise from
use of this instrument.

2.1 About this user manual

This manual provides information about the EZ1 Advanced.
It guides you systematically through the following sections:
1. Safety Information
2. Introduction
3. General Description
4. Installation Procedures
5. General Operation
6. Preventive Maintenance
7. Troubleshooting
8. Glossary
Appendices
The Appendices contain the following:
„ Contact information for technical assistance
„ Technical data
„ Warranty terms

2.2 General information

2.2.1 Technical assistance
At QIAGEN we pride ourselves on the quality and availability
of our technical support. Our Technical Service Departments
are staffed by experienced scientists with extensive practical
and theoretical expertise in molecular biology and the use of
QIAGEN products. If you have any questions regarding the

EZ1 Advanced User Manual 05/2008 2-1

Introduction

EZ1 Advanced or QIAGEN products in general, please do

not hesitate to contact us.
QIAGEN customers are a major source of information
regarding advanced or specialized uses of our products. This
information is helpful to other scientists as well as to the
researchers at QIAGEN. We therefore encourage you to
contact us if you have any suggestions about product
performance or new applications and techniques.
For technical assistance and more information, please see
our Technical Support Center at www.qiagen.com/Support
or call one of the QIAGEN Technical Service Departments or
local distributors (see back cover or visit www.qiagen.com ).

2.2.2 Policy statement

It is the policy of QIAGEN to improve products as new
techniques and components become available. QIAGEN
reserves the right to change specifications at any time.
In an effort to produce useful and appropriate
documentation, we appreciate your comments on this
publication. Please contact QIAGEN Technical Services.

2.2.3 Version management

This document is the EZ1 Advanced User Manual, revision
R1.

2.3 Intended use of the EZ1 Advanced

The EZ1 Advanced is designed to perform automated
isolation and purification of nucleic acids.
It is intended to be used only in combination with QIAGEN
kits indicated for use with the EZ1 Advanced instrument for
the applications described in the kit handbooks.
The system is intended for use by professional users, such as
technicians and physicians trained in molecular biological
techniques and the operation of the EZ1 Advanced.

2-2 EZ1 Advanced User Manual 05/2008

 Introduction

2.3.1 Requirements for EZ1 Advanced users

This table covers the general level of competence and
training necessary for transportation, installation, use,
maintenance, and servicing of the EZ1 Advanced.

Type of task Personnel Training and experience

Delivery No special No special requirements

requirements

Installation Laboratory Appropriately trained and

technicians or experienced personnel
equivalent familiar with use of computers
and automation in general

Routine use Laboratory Appropriately trained and

(running technicians or experienced personnel
protocols) equivalent familiar with use of computers
and automation in general

Regular, daily, Laboratory Appropriately trained and

and weekly technicians or experienced personnel
maintenance equivalent familiar with use of computers
and automation in general

Servicing and QIAGEN

annual preventive Instrument
maintenance Service
Specialists only

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Introduction

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 General Description

3 General Description
The EZ1 Advanced performs fully automated nucleic acid
purification from up to 6 samples using magnetic particles.
The automated steps include:
„ Reading reagent and sample information with a
handheld bar code scanner connected to the EZ1
Advanced
„ Lysis of samples
„ Binding of nucleic acids to magnetic particles
„ Washing and elution of nucleic acids
„ Generating a report file that either will be transmitted to
a PC or printed on an external printer after the protocol
run is finished
„ Using UV light for decontamination
The user inserts an EZ1 Advanced Card containing
protocol(s) into the EZ1 Advanced. After starting worktable
setup using the control panel and bar code reader, the user
loads samples, reagent cartridges, filter-tips in tip holders,
and elution tubes onto the EZ1 Advanced worktable.
The user then closes the EZ1Advanced door and starts the
protocol. The door locks automatically at the start of the
protocol. The protocol provides the necessary instructions for
the EZ1 Advanced to carry out automated nucleic acid
purification.
The aspiration and dispensing of samples and reagents and
the separation of magnetic particles are performed by the
6-channel pipettor head. The temperature of samples is
regulated by a heating system.

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General Description

3.1 External features of the EZ1 Advanced

Front view of the EZ1 Advanced.

1 3
EZ1 Advanced door EZ1 Advanced Card slot
2
Control panel 4
Status LEDs

3.1.1 EZ1 Advanced door

The EZ1 Advanced door can be manually opened by the user
in order to gain access to the worktable when no protocol is
running. A protocol cannot be started until the EZ1 Advanced
door is closed.

3.1.2 Control panel

The control panel is the user interface that allows the user to
operate the EZ1 Advanced. It consists of a keypad and a
vacuum fluorescent display (VFD).

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 General Description

Keypad
The following keys of the control panel are available to the
user.

Key Description

0–9 Selects a menu.

START Initiates an action or starts a protocol.

STOP Interrupts a protocol run.

ESC Displays the previous menu or text.

Up/Dn Up and down arrow key used, for

example, in the system setup menu.

ENT Enter key used, for example, in the system

setup menu to acknowledge data entry.

BS Backspace key used to remove incorrectly

entered values during data entry.

SHIFT + Used to enter the installation date.

Up/Dn

The other keys of the control panel are for service personnel
only.

Vacuum fluorescent display (VFD)

The display consists of 4 lines with 20 characters per line.

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General Description

Vacuum fluorescent display (VFD).

3.1.3 EZ1 Advanced Card slot

The EZ1 Advanced Card slot accepts the EZ1 Advanced
Card. Each EZ1 Advanced Card contains one or more
protocols that allow the EZ1 Advanced to perform nucleic
acid isolation.

CAUTION Damage to the instrument [C3]

Only use QIAGEN EZ1 Advanced Cards with the EZ1
Advanced. Other EZ1 Cards cannot be used with the
EZ1 Advanced.

Note: Do not insert the EZ1 Advanced Card while the EZ1
Advanced is switched on. EZ1 Advanced Cards must not be
exchanged while the EZ1 Advanced is switched on. Take care
not to expose the EZ1 Advanced Card to electric shock,
water, or dirt.

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 General Description

EZ1 Advanced Card inserted into EZ1 Advanced Card slot.

3.1.4 Status LEDs

There are 2 light-emitting diodes (LEDs) at the front of the
EZ1 Advanced:
„ Green LED — indicates that the instrument is receiving
power
„ Red LED — indicates that an error has occurred

3.1.5 Connector panel

The connector panel is located at the rear of the EZ1
Advanced.

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General Description

Connector panel.

It contains the power switch, the socket for the power cord,
the fuse box, and two RS232 connectors. The left connector
is used to connect the bar code reader. The right connector is
used to connect the EZ1 Advanced to a PC or an RS232
printer (optional).

3.2 Internal features of the EZ1 Advanced

The interior of the EZ1 Advanced contains the worktable and
the pipettor head.

3.2.1 Worktable
The worktable contains various racks for holding the
labware, samples, and reagent cartridges that are required
for the protocol run. The worktable also contains a heating
system for controlling the temperature of samples.

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 General Description

Pipettor head

Heating system

Reagent cartridges in
cartridge rack

Sample tubes

Tip holders with tips

in tip rack

Elution tubes

The EZ1 Advanced worktable.

Tip rack
This rack is located at the front of the worktable. The front
row holds up to six 1.5 ml elution tubes. The recommended
elution tubes are screw-capped, made of polypropylene,
supplied by Sarstedt (cat. no. 72.692), and provided in EZ1
Kits.
The next 2 rows hold up to 12 tip holders containing filter-
tips. (Note: Some protocols require only one row of filter-
tips.) The remaining row holds up to 6 sample tubes. Sample
tubes are 2 ml in volume, screw-capped, made of
polypropylene, supplied by Sarstedt (cat. no. 72.693), and
provided in EZ1 Kits.

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General Description

Tip rack.

Both tip holders and filter-tips are made of polypropylene

and are provided in EZ1 Kits. The filter-tips have a capacity
of 50–1000 μl.

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 General Description

Tip holders and filter-tips.

Cartridge rack
This rack is located behind the tip rack and holds up to
6 reagent cartridges. Insert the cartridge rack with the arrow
pointing towards the instrument.

Cartridge rack.

Reagent cartridges are made of polypropylene, contain

prefilled reagents, and are available from QIAGEN.

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General Description

Heating
positions

Reagent wells

Reagent cartridges.

A reagent cartridge consists of 10 reagent wells and

2 heating positions. One heating position is a well, the other
is a slot that can accept a tube.

Heating system
The heating system is located under the far end of the
cartridge rack. It holds the heating positions of the reagent
cartridges.

Tray
The tray is located under the racks and collects any drops of
liquid that may fall.

Tray.

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 General Description

3.2.2 Pipettor head

The pipettor head is mounted above the worktable and
moves in the Z direction (i.e., up and down) in order to reach
the samples and reagents on the worktable. The worktable
itself moves in the Y direction (i.e., front to back) in order to
present different samples and reagents under the pipettor
head.
The pipettor head contains 6 high-precision syringe pumps
that are connected to tip adapters that can be attached to
filter-tips. The syringe pumps operate simultaneously to allow
aspiration or dispensing of small volumes of liquid (50–
1000 μl) via the filter-tips.
The pipettor head also contains a magnet whose distance
from the attached filter-tips can be varied. This feature allows
the capture of magnetic particles that are present in the
liquid aspirated into the filter-tips.
Located behind the tip adapters is the piercing unit, a row of
6 metal spikes for puncturing the foil that covers the reagent
cartridges.
During operation, the piercing unit, automatically controlled
by the EZ1 Advanced, first exposes the reagents in the
reagent cartridges. The pipettor head then automatically
picks up filter-tips from the tip rack and performs aspiration
and dispensing operations at different locations on the
worktable before ejecting the tips back into the tip rack.

Pipettor head.

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General Description

3.2.3 UV lamp
The EZ1 Advanced is equipped with a UV lamp, located
under the blue EZ1 Advanced door. The UV lamp can be
manually switched on and off in a separate menu, as
described later in this manual.
Do not touch the UV lamp with your fingers since it may
reduce the lamp’s lifetime. The EZ1 Advanced door will be
locked during a UV light cycle in order to protect the
operator from UV radiation. The EZ1 Advanced will provide
a warning when the lifetime of the lamp is going to expire.
Call QIAGEN Technical Services when a UV lamp needs to
be replaced.

WARNING The EZ1 Advanced contains a UV lamp [W12]

Avoid looking directly into UV light. Do not expose your
skin to UV light.

CAUTION Lifetime UV lamp [C4]

The UV lamp needs a minimum switch-on time of
20 minutes. Do not interrupt a UV light cycle before 20
minutes have passed since it will reduce the lamp’s
lifetime.

UV lamp and reflector case.

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 General Description

UV lamp

Location of UV lamp under the EZ1 Advanced door.

CAUTION Cover of UV lamp [C5]

Be careful not to damage the cover of the UV lamp
when loading and unloading the worktable.

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General Description

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 Installation Procedures

4 Installation Procedures
The EZ1 Advanced is a plug-and-play instrument, making
unpacking and installation easy. A member of your group
who is familiar with laboratory equipment should oversee the
installation.

4.1 Requirements
Site
The EZ1 Advanced must be located out of direct sunlight,
away from heat sources, and away from sources of vibration
and electrical interference. Refer to Appendix A for the
operating conditions (temperature and humidity).
Use a level workbench that is large and strong enough to
accommodate the EZ1Advanced. Refer to Appendix A for the
weight and dimensions of the EZ1 Advanced.
The EZ1 Advanced must be placed near to a properly
grounded (earthed) AC power outlet. The power line to the
instrument should be voltage regulated and surge protected.

4.2 AC power connection

Power requirements
The EZ1 Advanced operates at:
„ 100–120 V AC ± 10%, 50/60 Hz, 240 VA
„ 200–240 V AC ± 10%, 50/60 Hz, 240 VA
Make sure the voltage rating of the EZ1 Advanced is
compatible with the AC voltage available at the installation
site.

Grounding requirements
To protect operating personnel, the EZ1 Advanced must be
correctly grounded (earthed). The EZ1 Advanced is equipped
with a 3-conductor AC power cord that, when connected to
an appropriate AC power outlet, grounds (earths) the EZ1

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Installation Procedures

Advanced. To preserve this protection feature, do not

operate the EZ1 Advanced from an AC power outlet that has
no ground (earth) connection.

Installation of AC power cord

Connect one end of the AC power cord to the socket located
on the rear of the EZ1 Advanced and the other end to the
AC power outlet.
The fuse box of the EZ1 Advanced is located under the socket
for the power cord and contains 2 fuses, which are labeled
as follows:
„ 110–120 V — a 6.3 A (250 V) time-delay fuse
compatible with 100–120 V power supplies
„ 220–240 V — a 3.15 A (250 V) time-delay fuse
compatible with 200–240 V power supplies
Before connecting the instrument to the power supply, you
may need to rotate the fuse box in order to select the correct
fuse. A fuse is correctly selected when its label is readable
and closer to the bottom of the instrument.

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 Installation Procedures

Selecting the correct fuse for Selecting the correct fuse for
200–240 V line power supply. 100–120 V line power supply.

WARNING Electrical hazard [W13]

Never install a fuse different from that specified in the
user manual.

4.3 Hardware installation

The following items are delivered:
„ EZ1 Advanced
„ International power cord set (5 countries)

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Installation Procedures

„ PC data cable
„ Cartridge rack
„ Tip rack
„ Tray
„ O-ring (pack of 6) and silicon grease
„ Fuses (1 each: 6.3 A and 3.15 A)
„ Bar code reader
„ EZ1 Advanced User Manual
„ EZ1 Advanced teaching data sheet
„ CD with EZ1 Advanced Communicator software

4.3.1 Unpacking the EZ1 Advanced

1. Cut and remove the plastic straps.

Plastic straps

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 Installation Procedures

2. Remove the outer top cardboard.

Outer top
cardboard

Disposables
and
accessories
Cardboard
tray

Corner shock
absorbers

Outer
cardboard
box

Wrapped EZ1
Advanced
Aluminum
strap
Corner shock
absorbers

Outer bottom
cardboard

3. Take out the packages with the disposables and

accessories.
4. Remove the cardboard tray.
5. Pull out the 4 corner shock absorbers at the top, and
remove the outer cardboard box by pulling it up.
6. Take out the wrapped EZ1 Advanced, and remove the
aluminum strap.

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Installation Procedures

7. Remove the inner top cardboard, and remove the EZ1

Advanced from the inner cardboard box.

Inner top
cardboard

EZ1
Advanced

Inner
cardboard
box

8. Remove all plastic sheets.

EZ1
Advanced
Plastic sheets

Inner bottom
cardboard

Note: Be careful not to damage the surface of the EZ1

Advanced after removing the plastic sheets.
Note: It is recommended to save the original packaging
material for later transportation of the EZ1 Advanced.

Removing the protectors for the Y- and Z-axes

During transportation, protectors prevent the moveable parts
of the EZ1 Advanced from moving along the Y- and Z-axes.
Before using the instrument, these protectors must be
removed.
1. Cut the cable ties.
2. Remove tape.
3. Remove the bubble wrap and the silica gel.

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 Installation Procedures

Cable ties

Bubble wrap

Silica gel

4. Push the pipettor head upward.

Pipettor head

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Installation Procedures

5. Remove the bag of foam packaging material.

Bag

Note: It is recommended to save the original packaging

material for later transportation of the EZ1 Advanced.

4.3.2 Installing the EZ1 Advanced

1. Adjust the fuse box if necessary (Section 4.2, page 4-1).
2. Connect the EZ1 Advanced to the power cord (Section
4.2, page 4-1).
3. Connect the handheld bar code reader to the EZ1
Advanced (Section 4.3.5, page 4-10).
4. Connect the PC to the EZ1 Advanced. Use the RS232
cable and plug it into the R232 connector labeled
“PC/Printer” at the rear of the instrument (Section 3.1.5,
page 3-5). Make sure that the connector is secured with
the 2 screws provided.
5. Optional: If you want to use the external printer as the
output device for the report file, connect the printer to the
EZ1 Advanced (Section 4.3.4, page 4-10).
6. Insert an EZ1 Advanced Card into the EZ1 Advanced
Card slot (Section 5.2.1, page 5-2).
7. Switch on the EZ1 Advanced. Always make sure that the
door is closed before switching on the EZ1 Advanced.
During operation, the door is locked magnetically by a
solenoid. During start up, this solenoid is tested in order

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 Installation Procedures

to check its functionality. The door must be closed for this

check.

4.3.3 Configuring and setting up the EZ1 Advanced

Setting the installation date

The first time the EZ1 Advanced is switched on, it is necessary
to enter the installation date. This date will be recorded in the
report file described below. The installation date is also used
to let the instrument know when to show the weekly and
annual maintenance reminders.

SERV: SETUP
SYSTEM
Installation date
DD MM YYYY
Key:Up,Dn,SHIFT,ENT

Set the day, month, and year. Press “SHIFT”-down arrow to

move the cursor to the right, from DD (day) to MM (month) to
YYYY (year). Press “SHIFT”-up arrow to move the cursor left,
from YYYY to MM to DD.
Press the up arrow or down arrow to increase or decrease
the value of the selected field.
After setting the date, store the setting by pressing “ENT”.
If you accidentally set the wrong date, call QIAGEN
Technical Services for assistance.

Setting the serial port

Set the serial port accordingly for use with a Star printer
SP500 or a PC as the output device for the report file (Section
5.10.3, page 5-28).

Setting time and date

The EZ1 Advanced has an integrated clock and date device.
Check the time and date and reset if necessary (Section
5.10.1, page 5-26 and Section 5.10.2, page 5-27).

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Installation Procedures

Setting the preventive maintenance reminder

The EZ1 Advanced reminds you when the annual
maintenance is due. You can set the reminder to remind you
annually or semiannually (Section 5.10.4, page 5-29).

4.3.4 Installing the printer (optional)

Unpack the Star printer SP500 (available from QIAGEN, cat.
no. 9018464). Follow the supplier’s instructions for use to
carry out the following steps.
„ Insert the fabric ribbon
„ Insert the paper roll
„ Connect the printer’s data cable
„ Connect the printer to an appropriate AC power outlet
via the power cord
„ Switch on the printer
Use the appropriate power cord, included with the printer.
Use the data cable included with the printer, with a 9-pin
connector on one end and a 25-pin connector on the other
end.
Connect the printer’s data cable to the EZ1 Advanced. The
connector is located at the rear of the instrument, labeled
“PC/Printer” (Section 3.1.5, page 3-5). Make sure that the
connector is secured with the 2 screws provided.
Check that the printer is correctly connected and working
properly (Section 5.9.3, page 5-23).

4.3.5 Installing the bar code reader

The bar code reader is powered through the data cable.
Unpack the bar code reader. Connect the cable to the bar
code reader.

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 Installation Procedures

Connect the bar code data cable to the EZ1 Advanced. The
connector is located at the rear of the instrument, labeled
“Barcode Reader” (Section 3.1.5, page 3-5). Make sure that
the connector is secured with the 2 screws provided. Switch
on the EZ1 Advanced.
The data communication between the reader and the EZ1
Advanced is RS232. This must first be recognized by the bar
code reader. To configure the bar code reader for RS232,
scan the configuration bar code on the plastic bag of the
RS232 data cable or scan the configuration bar code shown
below.

Configuration bar code RS232.

Check if the bar code reader is correctly connected and

working properly (Section 5.9.3, page 5-23).

4.4 Installing the PC software

The EZ1 Advanced software “EZ1 Advanced Communicator”
needs to be installed on the PC. The software is needed to
receive report files from EZ1 Advanced and to store data on
the PC hard disk.

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Installation Procedures

4.4.1 System requirements

„ IBM® compatible PC (e.g., from QIAGEN, cat. no.
9016319, and monitor, cat. no. 9016308)
Note: To operate multiple (up to 4) EZ1 Advanced
instruments with a single PC, only the QIAGEN PC (cat.
no. 9016319) can be used.
„ Operating system: Windows® XP service pack 2 or
Windows Vista
„ USB-Port: USB 1.1 or higher
„ Microsoft.NET Framework V1.1 (freely available for
download at www.microsoft.com ) installed on the PC
The instructions in Sections 4.4.2 and 4.4.3 refer to the
Windows XP operating system. Specifications for Windows
Vista are the similar, but the appearance and some
parameters may vary.

4.4.2 Installing the driver of the USB-RS232 converter

Installing the driver

If the PC has no RS232 port, first install the driver of the
USB–RS232 converter on the PC. Insert the CD and follow
the directions given.
The following installation wizard window appears.

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 Installation Procedures

Mapping the COM port

„ Click the “Start” button on your PC and select
“Settings/Control Panel”.
„ Double-click “System”.
„ Click the “ Hardware” tab and select the “Device
Manager” button.

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Installation Procedures

„ Expand “Ports” to show all available COM ports.

„ Connect the converter to an open USB port on the PC.
The device manager should then automatically update
the COM port list. Look for the COM port named “USB
to Serial Bridge”.

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 Installation Procedures

„ Right click on this COM port and select “Properties”.

Click the “Port Setting” tab and select the “Advanced”
button.

„ Change the dialog field “COM Port Number” to

“COM1”. The USB port is now mapped to COM port 1.
Always use COM Port 1 to work with the EZ1 Advanced.

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Installation Procedures

4.4.3 Installing the EZ1 Advanced Communicator

software
„ Insert the CD labeled “EZ1 Advanced Communicator
Software” into the PC. The software will automatically
start setup.exe .
„ The installation wizard will guide you through the
installation process.
„ After installation, open the EZ1 Advanced Communicator
software by double-clicking the EZ1 icon in the lower
system bar.

The user interface of the “EZ1 Advanced Communicator”

opens.
„ Select “Options” and open the “Serial port” window.
Check the “COM1” box.

„ The EZ1 Advanced Communicator software is now ready

to receive data from the EZ1 Advanced.
„ In order to check the connectivity between the PC and
EZ1 Advanced, use the test described in Section 5.9.3.

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 Installation Procedures

4.5 Installing multiple EZ1 Advanced

instruments
Up to 4 EZ1 Advanced instruments can be connected to a
single PC. For this application, only the QIAGEN PC
(cat. no. 9016643) can be used. This PC is equipped with a
1 x 4 Port Serial board. To set up your PC, follow the
directions given below.
„ Connect the large connector of the 4-fold multiple cable
to the rear side of the PC.

„ Connect the RS232 serial cables with the small

connectors of the 4-fold multiple cable. Use the screws to
secure the connection.
„ To connect 2 EZ1 Advanced instruments, use
connector P1 and P2.
„ To connect 3 EZ1 Advanced instruments, use
connector P1, P2, and P3.
„ To connect 4 EZ1 Advanced instruments, use
connector P1, P2, P3, and P4.

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Installation Procedures

The connectors are labeled on the top side of the

connector housing.

P1–P4

„ Connect the other end of the RS232 serial cables to the

EZ1 Advanced. See Section 3.1.5 for more information.
Use the connector for PC/printer.
„ If you use multiple EZ1 Advanced instruments, you do not
need to install the driver for the USB-RS232 converter.
„ Click the “Start” button on your PC and select
“Settings/Control Panel”.
„ Double-click “System”.
„ Click the “ Hardware” tab and select the “Device
Manager” button.
„ Expand “Ports” to show all available COM ports.
The following window will be displayed:

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 Installation Procedures

„ Make sure PCI Communication Ports COM 4 – COM 7

are displayed. These are the ports the PC uses to
communicate with the EZ1 Advanced instruments.
„ Install the EZ1 Advanced Communicator software by
following the instructions in Section 4.4.3.
„ Double-click the EZ1 icon in the lower system bar.
„ The user interface of the “EZ1 Advanced Communicator”
opens.
„ Select “Options” and open the “Serial port” window.

„ Depending on how many EZ1 Advanced instruments you

are using, select COM4 – COM7 starting with COM4.
„ The EZ1 Advanced Communicator software and the PC
Windows software on the PC are now configured for use
with multiple EZ1 Advanced instruments.
„ In order to check the connectivity between the PC and
EZ1 Advanced, use the test described in Section 5.9.3.

4.6 Transportation and removal of the EZ1

Advanced
If the EZ1 Advanced needs to be transported, please follow
your organization’s instructions and guidelines. In addition,
follow the steps below.

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Installation Procedures

1. Decontaminate the instrument.

2. Pack the EZ1 Advanced by following the steps of the
unpacking procedure described in Section 4.3.1 in
reverse order. Use original packaging material.
3. Appendix A gives information about transportation
conditions.
If the EZ1 Advanced needs to be sent to QIAGEN, please
contact your local instrument service organization. Follow the
steps below.
1. Decontaminate the instrument.
2. Follow the instruction of your local instrument service
organization.

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 General Operation

5 General Operation
This section describes how to operate the EZ1 Advanced.
Before proceeding, it is recommended that you familiarize
yourself with the features of the EZ1 Advanced by referring to
Section 3.
For information about disposable plasticware, chemicals,
and storage conditions, refer to the handbook of the
QIAGEN kit you are using.

5.1 Overview
The steps for operating the EZ1 Advanced are presented
below. Further details are provided later in this section.
1. Insert the EZ1 Advanced Card completely into the EZ1
Advanced Card slot.
2. Switch on the EZ1 Advanced.
3. After initialization, press the “START” key to start
worktable setup. The protocol will lead you through the
setup of the worktable by displaying messages in the
display.
For details, refer to the handbook supplied with the EZ1
Kit you are using.
4. If you want EZ1 Advanced to generate a report file, you
will be asked to enter additional data.
5. Open the EZ1 Advanced door, and set up the worktable
according to the messages shown in the display.
6. Close the EZ1 Advanced door.
7. Press “START” to start the protocol.
8. The EZ1 Advanced will automatically generate a report
file, if requested during setup, and send it either to the
PC or the printer.
9. At the end of the protocol run, you may optionally
choose to perform a UV decontamination run.

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General Operation

5.2 Inserting and removing the EZ1

Advanced Card
CAUTION Damage to the instrument [C6]
The EZ1 Advanced Card is an integrated part of the
system. Ensure that the EZ1 Advanced is always switched
off before you insert or remove the EZ1 Advanced Card.

5.2.1 Inserting the EZ1 Advanced Card

1. Open the cover of the EZ1 Advanced Card slot.

2. Insert the EZ1 Advanced Card into the EZ1 Advanced

Card slot.

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 General Operation

Orient the card so that the picture faces to the left and the
triangle symbol points towards the EZ1 Advanced Card slot.
Ensure that the card is completely inserted into the slot.

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General Operation

3. Close the cover of the EZ1 Advanced Card slot.

4. Switch on the EZ1 Advanced (Section 5.3.1, page 5-5).
Note: Do not remove the EZ1 Advanced Card while the
machine is switched on.

5.2.2 Removing the EZ1 Advanced Card

1. Switch off the EZ1 Advanced (Section 5.3.2, page5-5).
2. Turn the cover of the EZ1 Advanced Card slot.
3. Press the button at the bottom of the EZ1 Advanced Card
slot.

The EZ1 Advanced Card will be ejected.

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 General Operation

5.3 Switching the EZ1 Advanced on and off

5.3.1 Switching on the EZ1 Advanced
1. Insert the EZ1 Advanced Card (Section 5.2.1, page 5-2).
2. Switch on the EZ1 Advanced at the rear power switch.
3. The display shows the following screen for a few
seconds.

This text indicates that the EZ1 Advanced is initializing.

All modules move to their home positions.
4. After initialization, the main menu appears.

01 Apr 2008 10:30

START:Run 1:UV
2:Man 3:Test 4:Setup
Key:START,1,2,3,4

You can now operate the EZ1 Advanced.

5.3.2 Switching off the EZ1 Advanced

Switch off the EZ1 Advanced at the rear power switch.

5.4 Opening and closing the EZ1 Advanced

door
5.4.1 Opening the EZ1 Advanced door
Reach under the door and push it up until the upper end
slides into position. The door will be automatically held open.

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General Operation

5.4.2 Closing the EZ1 Advanced door

1. Press the door down to disengage the door lock.
2. Gradually lower the door until it rests on the worktable.
Take care that your fingers do not get caught between
the door and worktable.

CAUTION Damage to the instrument [C7]

Do not slam the door. It may harm the filament of the
UV lamp.

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 General Operation

5.5 Starting and stopping a protocol run

5.5.1 Starting and finishing a protocol run
After you have inserted the EZ1 Advanced Card and switched
on the EZ1 Advanced, start a protocol as follows.
1. Press “START” on the control panel.
2. The protocol will lead you through the setup of the
worktable by showing messages in the display.
Follow the instructions shown in the display. For details,
refer to the handbook supplied with the EZ1 Kit you are
using.
3. If you want the EZ1 Advanced to generate a report file,
you will be asked to enter additional data, such as user
ID, bar code of the kit, and bar code of the samples.
4. Open the EZ1 Advanced door, and set up the worktable
according to the messages shown in the display.
For details, refer to the handbook supplied with the EZ1
Kit you are using.
5. Close the EZ1 Advanced door.
The protocol run cannot start until the door is closed.
6. Press “START” to start the protocol run.
7. After the run is finished, remove the elution tubes, which
contain the purified nucleic acid samples. Remove
sample-preparation waste, and discard according to
your local safety regulations.
8. The EZ1 Advanced will automatically generate a report
file, if requested during setup, and send it either to the
PC or the printer.
9. At the end of the protocol run, you may optionally
choose to perform a UV decontamination run. For
details, refer to the handbook supplied with the EZ1 Kit
you are using.
10. Perform regular maintenance after each run as described
in Section 6.1, page 6-3.

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General Operation

5.5.2 Stopping a protocol run

While the protocol run is in the phase where data is being
read (e.g., sample tube bar code information), you can stop
the protocol run by following the instructions below.
1. Press “STOP”. The following screen appears.

====== PAUSE
=====
START: Continue
STOP: Stop
Key:START,STOP

2. Press “STOP” again to abort the protocol, or press

“START” to continue the protocol.
While the protocol run is in the phase where the instrument is
actively processing samples, you can abort the protocol run
by following the instructions below.
1. Press “STOP”. The following screen appears.

Do you really want to

abort the run ?

Key:START,STOP

Note that the protocol has not stopped yet.

2. Press “STOP” again to abort the protocol. The protocol
run will be invalid and will be marked accordingly in the
report file. The following screen appears.

01 Apr 2008 10:30

START:Run 1:UV
2:Man 3:Test 4:Setup
Key:START,1,2,3,4

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 General Operation

Alternatively, if you do not want to abort the protocol at

this point, press the “START” key. In this case, the
protocol run is not stopped and should not be affected.
3. After aborting a protocol, press “2” to display “Manual”
in the display. Press “2” again to return the tips to the tip
rack and to return the modules to their home positions.

5.6 Setting up the worktable

Note: For specific details about setting up the worktable,
refer to the onscreen instructions, which are also included in
the handbook supplied with the EZ1 Kit you are using.

5.6.1 Removing and replacing the tray

The tray is located under the worktable and can be removed
for cleaning.
1. Switch off the EZ1 Advanced.
2. Push the worktable toward the back of the EZ1
Advanced.
3. Remove the tray using its handle.

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General Operation

Reverse this procedure to replace the tray. To avoid

instrument damage, ensure that the tray is positioned
correctly.

5.6.2 Loading the reagent cartridges

Note: Do not remove the foil from the reagent cartridges.
1. Invert the reagent cartridges several times to mix the
magnetic particles. Use the same number of reagent
cartridges as the number of samples to be processed.
2. Tap the reagent cartridges until the reagents are
deposited at the bottom of the wells.
3. Remove the cartridge rack from the worktable.
4. Slide the reagent cartridges into the cartridge rack in the
direction of the arrow, as shown below, until you feel
resistance.

Press down the cartridges until they click into place.

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 General Operation

5. Return the cartridge rack to the worktable.

5.6.3 Loading the elution tubes, filter-tips, and sample

tubes
1. Remove the tip rack from the worktable.
2. Place the filter-tips into the tip holders.
Make sure that the tips are positioned correctly in the
holders.
3. Load the elution tubes, filter-tips, tip holders, sample
tubes, and any other equipment or reagents as described
in the messages shown in the display and described in
the relevant EZ1 Kit handbook.
Label the elution tubes before you load them into the
rack. Make sure that the caps of the tubes are removed
before you start the protocol.

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General Operation

Make sure that the caps of the sample tubes are

removed before you start the protocol.

4. Return the tip rack to the worktable.

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 General Operation

Make sure that the tips, tip holders, and tip rack are
correctly positioned on the worktable.

5.6.4 External tube rack

The external tube rack (cat. no. 9018484) is an accessory
that simplifies transfer of samples from primary tubes to the
sample tube rack.

5.7 Using the UV lamp

At the end of a protocol run, a display message will give you
the option of starting a UV decontamination run.
Alternatively, you can start a UV decontamination run
manually by switching the UV lamp on as described below.
Note: UV decontamination helps to reduce possible
pathogen contamination of the EZ1 Advanced worktable
surfaces. The efficiency of inactivation has to be determined
for each specific organism and depends, for example, on
layer thickness and sample type. QIAGEN cannot guarantee
complete eradication of specific pathogens.

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General Operation

5.7.1 Switching on the UV lamp

1. Make sure that the EZ1 Advanced door is closed. In the
main menu, press “1” to select the UV light function.

01 Apr 2008 10:30

START:Run 1:UV
2:Man 3:Test 4:Setup
Key:START,1,2,3,4
The following screen appears.

Decontamination
Set Time: 30 min.

Key:0-9,ENT,ESC

2. Use the keys “0” through “9” to set the duration of the
decontamination time. The minimum time is 20 minutes,
and the maximum time is 60 minutes. The default is
30 minutes. (Pressing “ESC”, will abort the procedure
and return you to the main menu.)
After setting a valid time, press the “ENT” key. The
following menu appears.

Decontamination
Time: 30 min.
Run:START
Key:START,ESC

If you enter an invalid decontamination time

(<20 minutes or >60 minutes), the following screen will
appear.

UV
DECONTAMINATION
Number must be
between 20 and 60
Key:ESC

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 General Operation

3. After entering a valid time, press “START” to switch on

the UV lamp.
The worktable will move slowly back and forth under the
UV light. During the UV run, the following screen
appears.

Decontamination>Run
TotalTime: TT min.
LeftTime : LL min.
Key:STOP

“TT” indicates the total time (in minutes), and “LL” indicates
the amount of time left.
At the end of the run, the following screen appears.

Decontamination>Run
UV lamp cooling

Please stand by

For user safety, the UV lamp cools for approximately

3 minutes. The EZ1 Advanced door cannot be opened until
after the cooling time has elapsed. After cooling, the main
menu appears.

5.7.2 Switching off the UV lamp

CAUTION Lifetime UV lamp [C4]
The UV lamp needs a minimum switch-on time of
20 minutes. Do not interrupt a UV light cycle before 20
minutes have passed since it will reduce the lamp’s
lifetime.

During a manual UV run, you can abort the run by pressing

the “STOP” key. The following screen appears.

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General Operation

Attention:
UV decontamination
not finished.
Continue:START/STOP

Press “STOP” again to abort the UV run. Alternatively,

pressing “START” will cause the UV run to continue.

5.7.3 Lifetime reminder of the UV lamp

The lifetime of the UV lamp is limited to 1200 cycles. The
instrument will remind you when the UV lamp will expire by
displaying the following screen.

UV Lamp Reminder:
UV lamp expires soon
UV runs left: CC
ENT=continue

Beginning 50 cycles before the 1200-cycle limit, the EZ1

Advanced will display this screen every time the instrument is
switched on. “CC” indicates the number of cycles remaining.
If this number is “0”, the UV lamp needs to be replaced.
Contact QIAGEN Technical Services for replacement of the
UV lamp.

5.7.4 UV lamp ignition error

If the UV lamp fails to ignite, the EZ1 Advanced will retry 2
more times. If the retries do not ignite the lamp, the following
error message appears.

ERROR:UV Lamp
UV Lamp did not
ignite
Key:ESC
Contact QIAGEN Technical Services if the lamp fails to
ignite.

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 General Operation

5.8 Manual operation

To start manual operation, press “2” in the main menu to
select the manual function.

01 April 2008 10:30

START:Run 1:UV
2:Man 3:Test 4:Setup
Key:START,1,2,3,4

The manual operation screen appears.

MANUAL OPERATION
1:Home 2:Return Tip
3:Clean 4:Resend
Key:1,2,3,4,ESC

5.8.1 Manual operation “home axis”

Using the “home axis” operation, all 4 modules or any one
selected module will move to their initial park positions.
Press ”1” in the manual operation screen to choose the
“home axis” operation. The following screen appears.

MANUAL OPERATION
home axis 0:ALL
1:Y 2:Z 3:P 4:M
Key:1,2,3,4,ESC

Press “0” to “4” to select the ALL, Y, Z, P, or M functions.

ALL: All modules will move to their park positions.
Y: The worktable moves to its park position.
Z: The nozzle unit will move to its park position.
P: The piercing unit will move to its park position.
M: The magnet unit will move to its park position.

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General Operation

The following screen appears.

MANUAL OPERATION
home axis Axis
START:Run
Key:START,ESC

“Axis” indicates either the ALL, Y, Z, P, or M function. Press

“START” to start the “home axis” operation or “ESC” to return
to the previous screen.
During operation, following screen appears.

MANUAL OPERATION
home axis Axis

Executing...

5.8.2 Manual operation “return tip”

Use the “return tip” operation to return any tips that are
attached to the tip adapter. The tips will be returned to the tip
rack.
Press ”2” in the manual operation screen to choose the
“return tip” operation. The following screen appears.

MANUAL OPERATION
return tip
START:Run
Key:START,ESC

Press “START” to start the “return tip” operation or “ESC” to

return to the previous screen.

5.8.3 Manual operation “clean”

Use the “clean” operation to get access to the piercing unit
for cleaning.

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 General Operation

The EZ1 Advanced door must initially be closed. Press ”3” in

the manual operation screen to choose the “clean”
operation. The following screen appears.

MANUAL OPERATION
clean piercing unit
START:Run
Key:START,ESC

Press “START” to start the “clean” operation or “ESC” to

return to the previous screen.
The EZ1 Advanced lowers the piercing unit. The following
screen appears.

MANUAL OPERATION
Open door and
clean piercing unit
ENT:Done

Open the EZ1 Advanced door, and clean the piercing unit as
described in Section 6.1, page 6-3. After cleaning the
piercing unit, close the EZ1 Advanced door and press “ENT”
to finish the procedure.

5.8.4 Manual operation “resend”

If a report file cannot be sent to the PC or printer, it is
temporarily stored on the EZ1 Advanced. Up to 10 report
files can be stored. Use the “resend” function to manually
transmit the report file(s) to the PC or printer.
Press ”4” in the manual operation screen to choose the
“resend” operation. The following screen appears.

MANUAL OPERATION
resend report file
START:Run
Key:START,ESC

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General Operation

Press “START” to start the “resend” operation or “ESC” to

return to the previous screen.
While the operation is in progress, the following screen
appears.

MANUAL OPERATION
resend report file

Executing...

Alternatively, if there are no report files temporarily stored on

the EZ1 Advanced, the following screen appears.

MANUAL OPERATION
No report file to
be sent
Key:ESC

Press “ESC” to return to the manual operation screen.

If there is an error during transmission, the following screen
appears.

MANUAL OPERATION
Resend report file
failed
Key:ESC

Press “ESC”. Check the connection to the PC or printer. Make

sure that the PC or printer is turned on. If using a PC, make
sure that the EZ1 Advanced Communicator software is
installed and running on the PC.
If the transmission problem cannot be fixed, contact QIAGEN
Technical Services.

5.9 Test
Press “3” in the main menu to select the test function.

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 General Operation

01 Apr 2008 10:30

START:Run 1:UV
2:Man 3:Test 4:Setup
Key:START,1,2,3,4

The test screen appears.

TEST
1:Axis 2:Temp
3:Serial 4:Version
Key:1,2,3,4,ESC

5.9.1 Test axis

Press ”1” in the test screen to choose the “Axis” operation.

TEST
1:Axis 2:Temp
3:Serial 4:Version
Key:1,2,3,4,ESC

The following test procedures will be carried out by the EZ1

Advanced.
„ Move modules to their home positions.
„ Pick up tip 2 and release it.
„ Pick up tip 1 and move it to sample position and Proc 1
position.
„ Move the magnet unit to “narrow” position and then
return to home.
„ Aspirate and dispense to test the pipetting unit.
„ Move to Proc 2–Proc 11 positions (see figure on next
page).
„ Move to elution position.
„ Release tip 1.

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General Operation

Proc 12

Proc 11

Proc 10
Proc 9
Proc 8
Proc 7
Proc 6
Proc 5
Proc 4
Proc 3
Proc 2
Proc 1

Sample

Tip 2

Tip 1

Eluate

Proc positions tested by the EZ1 Advanced test procedure.

5.9.2 Test heating block

This function tests if the heating block of the EZ1 Advanced is
operating.
Press ”2” in the test screen to choose the “Temp” operation.

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 General Operation

TEST
1:Axis 2:Temp
3:Serial 4:Version
Key:1,2,3,4,ESC

The following screen appears.

TEST: TEMPERATURE
set temp: SS.S C
START:Run
Key:Up,Dn,START,ESC

“SS.S” indicates the set temperature in degrees Celsius. Press

the up arrow or the down arrow to increase or decrease the
temperature. The upper limit is 99°C. Press “START” to start
the heating process.
The following screen appears.

TEST:
TEMPERATURE
Temp: ss.s C
actual: aa.a C r
Key:ESC

“ss.s” indicates the set temperature, and “aa.a” indicates the

current temperature. “r” indicates the result, showing “O” if
the temperature is within a given range or “X” if the
temperature is outside the given range.

5.9.3 Test serial port

Press ”3” in the test screen to choose the “Serial” operation.

TEST
1:Axis 2:Temp
3:Serial 4:Version
Key:1,2,3,4,ESC

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General Operation

The serial port test screen appears.

TEST: SERIAL PORT

1:PC/Printer
2:Bar code
Key:1,2,ESC

Press “1” to test the PC or printer communication. The

following screen appears.

TEST: PC/Printer
target: Type.
START:Run
Key:START,ESC

“Type” indicates whether the serial port is configured for a

PC (“PC”) or a printer (“Printer”) (see Section 5.10.3, page 5-
28).

PC
Press “START” to send a test string to the PC. The following
screen appears.

TEST: PC/Printer
target: PC
Result: PASSED
Key:ESC

If the transmission is successful, the result will show

“PASSED”; if not successful, it will show “FAILED”.

Printer
Press “START” to send a test string to the printer. The
following screen appears after transmission is complete.

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 General Operation

TEST: PC/Printer
target: Printer
Result: COMPLETED
Key:ESC

If successful, the following text is printed by the printer.

Printout of the printer test.

Bar code reader

Press “2” in the serial port test screen to test the bar code
reader.

TEST: SERIAL PORT

1:PC/Printer
2:Bar code
Key:1,2,ESC

Use the bar code reader to read a bar code (e.g., from the
Q-Card included in an EZ1 Kit). When a new bar code is
read, the previous bar code will be overwritten. A beep
sounds after a successful scan of the bar code.
The following screen appears, showing up to 25 digits.

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General Operation

TEST: Barcode
Result:
BBBBBBBBBBBB
BBBBBBBBBBBBB
Key:ESC

“B” indicates the individual digits of the bar code.

5.9.4 Test version

Press ”4” in the test screen to choose the “Version”
operation.

TEST
1:Axis 2:Temp
3:Serial 4:Version
Key:1,2,3,4,ESC

The following screen appears.

TEST: VERSION
Firmware: VersionNo

Key:ESC

“VersionNo” indicates the current version of the firmware.

Press “ESC” to return to the test screen.

5.10 System setup

5.10.1 Setting the date
Press “4” in the main menu to select system setup.

01 Apr 2008 10:30

START:Run 1:UV
2:Man 3:Test 4:Setup
Key:START,1,2,3,4

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 General Operation

The system setup menu appears.

SYSTEM SETUP
1:Date 2:Time
3:Serial Port 4:PM
Key:1,2,3,4,ESC

Press “1” to change the date.

The following screen appears.

SETUP: DATE
DD MM YYYY
Key:
Up,Dn,SHIFT,ENT,ESC

Set the day, month, and year. Press “SHIFT”-down arrow to

move the cursor right, from DD (day) to MM (month) to YYYY
(year). Press “SHIFT”-up arrow to move the cursor left, from
YYYY to MM to DD.
Press the up arrow or down arrow to increase or decrease
the value of the selected field.
After setting the date, store the setting by pressing “ENT”.
Alternatively, press “ESC” to leave the date unchanged.

5.10.2 Setting the time

Press ”2” in the system setup menu to change the time.

SYSTEM SETUP
1:Date 2:Time
3:Serial Port 4:PM
Key:1,2,3,4,ESC

The following screen appears.

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General Operation

SETUP: TIME
HH:MM
Key:
Up,Dn,SHIFT,ENT,ESC

Press “SHIFT”-down arrow to move the cursor right, from HH

(hours) to MM (minutes). Press “SHIFT”-up arrow to move the
cursor left, from MM to HH.
Press the up arrow or down arrow to increase or decrease
the value of the selected field.
After setting the time, store the setting by pressing “ENT”.
Alternatively, press “ESC” to leave the time unchanged.

5.10.3 Setting the serial port

This setting configures the serial port for use with a printer or
a PC as the output device for the report file.
Press ”3” in the system setup menu to change the setting of
the serial port.

SYSTEM SETUP
1:Date 2:Time
3:Serial Port 4:PM
Key:1,2,3,4,ESC

The following screen appears.

SETUP: SERIAL
PORT
Current: CS
Set : NS
Key:Up,Dn,ENT,ESC

“CS” (current setting) indicates the current setting of the serial

port as “PC”, “Printer”, or “Not Used”.
Press the up arrow or the down arrow to change the “NS”
(new setting) field to “PC”, “Printer”, or “Not Used”.

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 General Operation

Store the new setting by pressing “ENT”. Alternatively, press

“ESC” to leave the current setting unchanged.

5.10.4 Setting the preventive maintenance reminder

Press ”4” in the system setup menu to change the setting of
the preventive maintenance reminder.

SYSTEM SETUP
1:Date 2:Time
3:Serial Port 4:PM
Key:1,2,3,4,ESC

The following screen appears.

SETUP: REMINDER
PM Interval

Key:Up,Dn,ENT,ESC

Press the up arrow or the down arrow to change the

“Interval” field to “1/2 year” (semiannual) or “1 year”
(annual).
After setting the new interval, store the setting by pressing
“ENT”. Alternatively, press “ESC” to leave the current interval
unchanged.

5.11 Using the bar code reader

The bar code reader is preset to read the following types of
bar codes.
„ 2/5 interleaved
„ EAN Family
„ Code 39
„ Code 128
To read a bar code, press the button located on the inner
side of the handle of the bar code reader. A red light
appears. Hold the bar code reader in front of the bar code
at a distance of approximately 20 mm. A beep will sound to

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General Operation

confirm that the bar code has been read. The bar code will
be displayed on the screen.

5.12 Using the external printer (optional)

The EZ1 Advanced allows you to confirm that a report was
printed correctly before the report file is deleted. The
following screen appears.

SEND REPORT
Print out o.k ?
1=o.k, 2=not o.k
Key:1,2,ESC

Press “1” to confirm that the print quality is satisfactory. The

report file in the EZ1 Advanced will then be deleted.
Alternatively, press “2” if the print quality is not satisfactory.
The report file will be printed again.
An example report file is shown on the next page.

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 General Operation

Example printout of a report file.

Note: If the printer is turned on before switching on the EZ1

Advanced, some additional characters will be printed before
the report. To avoid this, switch on the EZ1 Advanced before
switching on the printer.

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General Operation

5.13 Generating a report file

The EZ1 Advanced will generate a report file if this option
was selected during the protocol run.
The report file contains the fields described on the next
pages.

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 General Operation

Parameter Example output Description

REPORT - FILE Title of report file

EZ1 Advanced:

Serial no. EZ1 0301F0172 Serial number stored on the EZ1

Advanced: Advanced

User ID: 9267 User ID, set during protocol run

Can be a number or a name
scanned with the bar code reader
Maximum of 9 characters

Firmware version: V1.0.0 Current firmware version

Installation date Apr 01, 2008 Installation date, set the first time
of instr.: the EZ1 Advanced is powered
Stored permanently on the EZ1
Advanced

Weekly May 10, 2008 When you accept the weekly

maintenance maintenance reminder, the date is
done on: stored and is indicated here

Yearly Apr 25, 2008 When you accept the annual

maintenance maintenance reminder, the date is
done on: stored and is indicated here

Date of last UV- May 01, 2008 Date of last UV run recorded and
run: stored

Start of last UV- 14:04 Start time of last UV run

run:

End of last UV- 14:34 End time of last UV run

run:

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General Operation

Parameter Example output Description

Status of last UV- o.k. Status of the last UV run can show
run: „ o.k.
„ UV run aborted
„ UV lamp expired
„ UV lamp broken

Protocol name: Investigator Protocol name stored on EZ1

Advanced Card and copied into
report file

Trace Additional protocol name (specifies

the protocol if there are multiple
protocols on the EZ1 Advanced
Card)

Date of run: May 14, 2008 Time stamp for date of run given
through internal clock and
calendar

Start of run: 15:13 Time stamp for start of run given

through internal clock and
calendar

End of run: 15:43 Time stamp for end of run given

through internal clock and
calendar

Status run: o.k. The status of the run can show

„ o.k.
„ not o.k.
„ aborted

Error code: 21 See section 7.2 for error codes

Sample input 300 Sample input volume in microliters,

volume[ul]: depending on the protocol

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 General Operation

Parameter Example output Description

Elution 50 Elution volume in microliters,

volume[ul]: depending on the protocol

Channel A: Information for channel A begins

here

Sample ID: 8730 Sample ID, defined by the bar

code system of the user

Reagent kit 9900201 Reagent kit number defined by the

number: Q-Card

Reagent lot 1151234567 Reagent lot number defined by the

number: Q-Card

Reagent expiry Jan 14, 2009 Reagent expiry/expiration date

date:

Assay kit ID: 0472 Assay kit ID (optional)

Note: 8432 Optional information, such as a

name scanned by the bar code
reader

Channel B: Information about channels B–F

begins here (same format as
above for channel A)

If the report file could not be transmitted (e.g., if the serial

connection is broken), the report will remain stored on the
EZ1 Advanced. After connection is reestablished, use the
manual transmit function to resend the report file (see
Section 5.8.4, page 5-19).
Up to 10 report files can be stored temporarily on the EZ1
Advanced. When additional files are stored, the oldest report
files will be deleted. The following screen appears.

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General Operation

Caution: Memory full

Oldest report file
will be erased
1:Cont ESC:abort run

Press “1” to delete the oldest report file and continue the
protocol run. Alternatively, press “ESC” to abort the protocol
run.

5.14 Using the EZ1 Advanced Communicator

software
EZ1 Advanced Communicator is a software program that
runs on a PC. The software receives the report file and stores
it in a folder that you define. After the PC has received the
report file, you can use and process the file with a LIMS
(Laboratory Information Management System) or other
programs.

5.14.1 User interface

The main window shows the list of report files received. Click
the “Refresh list” button to update the list.
Select a file by single-clicking on the name. The software will
perform a checksum test on the file. The result is shown
under “Validity check result”.

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 General Operation

The report file name contains the following components.

„ Title (e.g., EZ1_Report)
„ Serial number of the EZ1 Advanced
„ Date in format YYYY-MM-DD
„ Time in format HH-MM-SS
„ File extension *.csv (comma-separated value)
The date and time are when the report file was received by
the PC.
The “Status Message” panel displays the name of the last
report file that was sent.
Select “File” to exit the graphical user interface.
Select “Options” to display the following window showing the
EZ1 Advanced serial ports.

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General Operation

COM1 should already be selected.

You can check the serial settings by checking one of the
boxes. The following window opens.

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 General Operation

The default settings are as shown above: baud rate of 9600

bits/s, 8 data bits, parity none, 1 stop bit, flow control none.
Select “About” in the main window to show the version of the
EZ1 Advanced Communicator software.

Click the “Change path” button in the main window to

change the folder where the selected report file is stored. The
following window opens. Browse and select the folder.

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General Operation

5.15 Using multiple EZ1 Advanced instruments

Up to 4 EZ1 Advanced instruments can be operated with a
single PC. For this application, only the QIAGEN PC (cat. no.
9016643) can be used. The following combinations are
available from QIAGEN.

Product Contents Cat. no.

EZ1 Advanced DUO 2 EZ1 Advanced instruments, 1 PC 9001450

(with 4 serial ports)

EZ1 Advanced TRIPLEX 3 EZ1 Advanced instruments, 1 PC 9001451

(with 4 serial ports), 1 Printer

EZ1 Advanced 4 EZ1 Advanced instruments, 1 PC 9001452

QUATTRO (with 4 serial ports), 1 Printer,
1 External Tube Rack

5.16 Evaluating pipetting accuracy

The EZ1 Advanced Test Card (cat. no. 9018300) contains a
protocol that is designed to evaluate the pipetting accuracy
of the EZ1 Advanced. The EZ1 Advanced Test Card is
supplied with a QIAGEN Supplementary Protocol that guides
the user through the test step by step.
The EZ1 Advanced Test Card also contains protocols to
check the tightness of the tip adapters and to evaluate the
temperature accuracy of the EZ1 Advanced.

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 Preventive Maintenance

6 Preventive Maintenance
This table covers the types and frequencies of maintenance
required and the personnel required to carry out the
maintenance.

Type of task Frequency Personnel

Regular After each run on the EZ1 Laboratory technicians or

maintenance Advanced equivalent

Daily At the end of each day Laboratory technicians or

maintenance that the EZ1 Advanced is equivalent
run, after the regular
maintenance

Weekly Once per week, after the Laboratory technicians or

maintenance regular and daily equivalent
maintenance

Annual Annually or semiannually QIAGEN Instrument

preventive (depending on how the Service Specialists only
maintenance reminder was set, see
and servicing Section 5.10.4,
page 5-29)

Maintenance reminders
The EZ1 Advanced has a built-in clock and calendar device
to remind you when the weekly or annual maintenance is
due.
The weekly maintenance reminder screen appears.

Maintenance Reminder
Weekly
1: Done 2: Do later
Key:1,2

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Preventive Maintenance

If you have already completed the weekly maintenance,

press “1” to confirm. The current date will be stored in the
report file. See Section 6.3, page 6-7 for more details about
weekly maintenance.
If the weekly maintenance has not been completed, you can
press “2” to indicate that the maintenance will be performed
later. The next time the EZ1 Advanced is switched on, the
reminder screen will appear again.
The screen will continue to appear each time the EZ1
Advanced is switched on, until you press “1” to confirm that
the maintenance has been completed.
The same procedure applies for the annual maintenance.
The annual maintenance reminder screen appears.

Maintenance Reminder
Yearly
1: Done 2: Do later
Key:1,2

When this maintenance reminder appears, call your

QIAGEN Instrument Service Specialist for annual
maintenance. The annual maintenance reminder will appear
every 6 months if the interval was set to “1/2 year” (see
Section 5.10.4, page 5-29).
WARNING Risk of electric shock [W14]
Do not open any panels on the EZ1 Advanced.
Risk of personal injury and material damage
Only perform maintenance that is specifically described
in this manual.

Servicing
Each EZ1 Advanced is supplied with a one-year warranty that
includes all repairs due to mechanical breakdown.
Worldwide, the maximum time for response to a breakdown
is 5 days. Application development, software upgrades,
worktable accessories, disposable items, and replacement of

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 Preventive Maintenance

spare parts such as syringes, tubing, and pipet tips are not
included in the warranty.
QIAGEN offers comprehensive Service Support Agreements,
including IQ/OQ, Warranty Extensions, Full Cover Support
Agreements, and Preventive Maintenance Agreements.
Service Support Agreements ensure high performance from
your EZ1 Advanced. In addition, service histories are fully
documented and all parts are certified and guaranteed.
Contact your local QIAGEN Instrument Service
representative, or your local distributor for more information
about flexible Service Support Agreements from QIAGEN.

6.1 Regular maintenance procedure

Regular maintenance is required after each run on the EZ1
Advanced.
WARNING Risk of personal injury and material damage [W1]
Improper use of the EZ1 Advanced may cause personal
injuries or damage to the instrument.
The EZ1 Advanced must only be operated by qualified
personnel who have been appropriately trained.
Servicing of the EZ1 Advanced must only be performed
by QIAGEN Instrument Service Specialists.

WARNING Hazardous chemicals and infectious agents [W15]

The waste contains samples and reagents. This waste
may contain toxic or infectious material and must be
disposed of properly. Refer to your local safety
regulations for proper disposal procedures.

If working with potentially infectious materials, such as

human blood, serum, or plasma, the EZ1 Advanced system
should be decontaminated after use (Section 6.4, page 6-8).
After running a protocol, clean the piercing unit of the
pipettor head:
1. Remove sample-preparation waste, and discard
according to your local safety regulations.
2. Close the EZ1 Advanced door.

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Preventive Maintenance

3. Press “2” in the main menu to select the manual

function.
01 Apr 2008 10:30
START:Run 1:UV
2:Man 3:Test 4:Setup
Key:START,1,2,3,4

The manual operation screen appears.

MANUAL OPERATION
1:Home 2:Return Tip
3:Clean 4:Resend
Key:1,2,3,4,ESC

4. Press “3” to choose the “clean” operation. The following

screen appears.

MANUAL OPERATION
clean piercing unit
START:Run
Key:START,ESC

5. Press “START”.
The EZ1 Advanced will lower the piercing unit.
The following screen appears.

MANUAL OPERATION
Open door and
clean piercing unit
ENT:Done

6. Open the EZ1 Advanced door, and wipe the piercing

unit using a soft tissue moistened with 70% ethanol. The
piercing unit is sharp. Two pairs of gloves are
recommended.

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 Preventive Maintenance

Piercing
unit

7. Wipe the piercing unit using a soft tissue moistened with

distilled water.

8. Close the EZ1 Advanced door and press “ENT”.

The piercing unit returns to its original position.
The manual operation screen appears.

MANUAL OPERATION
1:Home 2:Return Tip
3:Clean 4:Resend
Key:1,2,3,4,ESC

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Preventive Maintenance

Note: If you press “ENT” while the EZ1 Advanced door is

open, an error message appears (see Section 7.1.1,
page 7-1).
9. Press “ESC” to return to the main menu.

01 Apr 2008 10:30

START:Run 1:UV
2:Man 3:Test 4:Setup
Key:START,1,2,3,4

10. Open the EZ1 Advanced door. Clean the tray and racks
with 70% ethanol and then with distilled water.
See Section 5.6.1, page 5-9 for removing the tray.
11. Clean the EZ1 Advanced worktable with 70% ethanol
and then with distilled water.
12. Wipe the other surfaces of the worktable with a diluted
neutral soap solution and then with distilled water.
You may now run another protocol or switch off the EZ1
Advanced.
13. Wipe the surface of the instrument and the blue door
using a soft tissue moistened with 70% ethanol.

6.2 Daily maintenance procedure

WARNING Hazardous chemicals and infectious agents [W15]
The waste contains samples and reagents. This waste
may contain toxic or infectious material and must be
disposed of properly. Refer to your local safety
regulations for proper disposal procedures.

After running the last protocol of the day, perform the daily
maintenance procedure:
1. Clean the piercing unit (Section 6.1, page 6-3).
2. Remove sample-preparation waste remaining, if
necessary, and discard according to your local safety
regulations.
3. Check that the tray is clean. If necessary, clean it with
70% ethanol, and then with distilled water.

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 Preventive Maintenance

See Section 5.6.1, page 5-9 for removing the tray.

4. Clean the worktable and its racks with 70% ethanol, and
then with distilled water.
5. Wipe the other surfaces of the EZ1 Advanced with diluted
neutral soap solution, and then with water.
6. Wipe the O-rings of the tip adapters with a lint-free
tissue.

6.3 Weekly maintenance procedure

Perform the daily maintenance procedure before you
perform the weekly maintenance procedure.
To maintain good contact between tip adapters and filter-tips
and to prevent liquid leaking from the tips, grease the
O-rings of the tip adapters every week:
1. Apply a small amount of silicon grease to the end of a
filter-tip.
2. Apply the silicon grease to the surface of the O-rings.
3. Place the tip onto the pipettor head, and rotate the tip on
the pipettor head to distribute the silicon grease evenly.

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Preventive Maintenance

O-ring

Gap

Note: The filter-tips should sit flush against the upper

white plastic bar if the O-rings are properly greased.
There should not be a gap. Excess or insufficient grease
can affect the performance of the EZ1 Advanced.

6.4 Reagents for decontamination

The following disinfectants and detergents are compatible
with metallic surfaces and removable components of the EZ1
Advanced system. Use according to manufacturers’
instructions for effective disinfection.
WARNING Toxic fumes [W16]
Do not use bleach to clean or disinfect the instrument.
Bleach in contact with salts from the buffers can produce
toxic fumes.
„ Mikrozid® Liquid (Schülke & Mayr GmbH;
www.schuelke-mayr.com )* — ethanol-based disinfectant
for cleaning surfaces, such as the worktable (consists of
25 g ethanol and 35 g 1-propanol per 100 g Mikrozid
Liquid)

6-8 EZ1 Advanced User Manual 05/2008

 Preventive Maintenance

„ Lysetol® AF or Gigasept® Instru AF (Schülke & Mayr

GmbH)* — quaternary ammonium solution for
submerging worktable items, such as holders (consists of
14 g cocospropylene-diamine-guanidine diacetate, 35 g
phenoxypropanols, and 2.5 g benzalkonium chloride per
100 g, with anticorrosion components, fragrance, and
15–30% nonionic surfactants)
Note: If you want to use disinfectants different from those
recommended, ensure that their compositions are similar to
those described above. A suitable alternative to Mikrozid
Liquid is Incidin Liquid (EcoLab; www.ecolab.com ). A
suitable alternative to Lysetol AF or Gigasept Instru AF is
DECON-QUAT® 100 (Veltek Associates, Inc.;
www.sterile.com ).*
Note: If you are not sure about the suitability of disinfectants
or cleaning agents for use with the EZ1 Advanced, do not
use them.
Note: Do not spray cleaning or disinfectant liquids onto
surfaces of the EZ1 Advanced. Spray bottles should be used
only for items that have been removed from the EZ1
Advanced.

* This is not a complete list of suppliers and does not include many important
vendors of biological supplies.

EZ1 Advanced User Manual 05/2008 6-9

Preventive Maintenance

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6-10 EZ1 Advanced User Manual 05/2008

 Troubleshooting

7 Troubleshooting
7.1 Detecting an error
7.1.1 EZ1 Advanced door errors
If the EZ1 Advanced door is open when you start a protocol
run, the following error screen will be displayed.

ERROR:Door open!
Close the door.
Press ESC to retry.
Key:ESC

Close the door and press “ESC” to continue with the protocol
run.
If the door sensor indicates that the EZ1 Advanced door is
closed but the locking mechanism is defective, the following
error screen will be displayed.

ERROR:Cannot lock
Check the door.
Press ESC to retry.
Key:ESC

Press “ESC” to retry. If the error persists, contact QIAGEN

Technical Services.

7.1.2 Other errors

If a protocol run is interrupted due to an error:
„ The red LED flashes
„ An alarm sounds
„ The display will show an error message

EZ1 Advanced User Manual 05/2008 7-1

Troubleshooting

ERROR:ErrCode
Line:LineNo

Key:ESC

The first line shows the Error Code. Refer to the error code
list in section 7.2 for more information about the error.
The second line indicates the line number of the protocol at
which the error occurred.
Record the error code and the line number, and contact
QIAGEN Technical Services. Then reset the EZ1 Advanced by
following the procedure given below.
1. Press “ESC” to display the main menu.
2. Ensure that the EZ1 Advanced door is closed.
3. Press “2” to select the manual function.

01 Apr 2008 10:30

START:Run 1:UV
2:Man 3:Test 4:Setup
Key:START,1,2,3,4

4. Press “2” to return the tips to the tip rack and to return
the modules to their home positions.

MANUAL OPERATION
1:Home 2:Return Tip
3:Clean 4:Resend
Key:1,2,3,4,ESC

5. Press “ESC” to return to the main screen.

Another protocol can now be run.
Note: It is not possible to continue a protocol run that has
been interrupted due to an error.

7-2 EZ1 Advanced User Manual 05/2008

 Troubleshooting

7.2 Error codes

Error code Description

10 Home sensor not activated during an operation other than a

protocol

11 End-limit sensor not activated during an operation other than

a protocol

12 Home sensor of the pipettor head (Z-axis) not activated

during a protocol run

13 Home sensor of the plunger unit (P-axis) not activated during

a protocol run

14 Home sensor of the magnet unit (M-axis) not activated during

a protocol run

15 Home sensor of the worktable (Y-axis) not activated during a

protocol run

16 End-limit sensor of the pipettor head (Z-axis) not activated

during a protocol run

17 –

18 –

19 End-limit sensor of the worktable (Y-axis) not activated during

a protocol run

20 Motor for pipettor head movement (Z-axis) does not respond

21 Motor for plunger/piercing unit movement (P-axis) does not

respond

22 Motor for magnet-unit movement (M-axis) does not respond

23 Motor for worktable movement (Y-axis) does not respond

during a protocol run

EZ1 Advanced User Manual 05/2008 7-3

Troubleshooting

Error code Description

24 Door open when starting an operation

25 Bottom sensor of pipettor head (Z-axis) activated during an

operation

26 Communication error between heating block and

temperature controller, or card is not an EZ1 Advanced Card

27 Communication error between motors and driver board

28 –

29 –

30 Protocol import error

31 Door lock error during operation

32–99 –

100 Protocol checksum error

101 VP table checksum error

102–109 –

110 System error

7-4 EZ1 Advanced User Manual 05/2008

 Glossary

8 Glossary
Term Description

Bar code A handheld device that enables scanning of bar codes

reader and conversion of them into data that is transmitted to
the EZ1 Advanced.

Cartridge rack A metal rack that accommodates reagent cartridges on

the worktable.

Connector The panel on the rear of the EZ1 Advanced. It contains

panel the power switch, the socket for the power cord, the fuse
box, and a connector for a computer cable.

Control panel The user interface that allows the user to operate the EZ1
Advanced. The control panel consists of a VFD and a
keypad.

Elution tube A polypropylene, screw-capped 1.5 ml tube for collecting

purified nucleic acids.

Error code A 2- or 3-digit number that indicates a specific error of

the EZ1 Advanced.

EZ1 Advanced A card that contains one or more protocols for the EZ1
Card Advanced and is inserted into the instrument.

EZ1 Advanced A slot at the front of the EZ1 Advanced that accepts an
Card slot EZ1 Advanced Card.

EZ1 Advanced A software program that runs on a PC and enables

Communicator reception and storage of report files from the EZ1
Advanced.

EZ1 Advanced The main door at the front of the EZ1 Advanced. When
door open, it provides complete access to the worktable.

EZ1 Advanced User Manual 05/2008 8-1

Glossary

Term Description

Filter-tip An item of labware that is picked up by a tip adapter

during operation of the EZ1 Advanced. Liquid is
aspirated into and dispensed from a filter-tip. A filter-tip
is also the location where separation of magnetic
particles occurs.

Heating A component of the EZ1 Advanced that accommodates

system the heating positions of the reagent cartridges and heats
samples.

O-ring A ring that is fitted at the bottom of a tip adapter. It is

required for good contact between the tip adapter and a
filter-tip.

Pipettor head The component of the EZ1 Advanced that aspirates and
dispenses liquid and separates magnetic particles. The
pipettor head moves up and down above the worktable
and contains 6 syringe pumps, each of which is
connected to a tip adapter.

Protocol A set of instructions for the EZ1 Advanced that allows the
instrument to automate a nucleic acid purification
procedure. Protocols are run using the control panel.

Reagent An item of labware that contains 10 wells and 2 heating

cartridge positions. One heating position is a well, the other is a
slot that can accept a tube. A reagent cartridge is
prefilled with reagents.

Report file A data file generated by the EZ1 Advanced containing

system and run parameters. The report file can be sent
directly to a printer or to a PC running EZ1 Advanced
Communicator software.

Sample tube A polypropylene, screw-capped 2 ml tube for holding a

sample containing nucleic acids to be purified.

8-2 EZ1 Advanced User Manual 05/2008

 Glossary

Term Description

Tip adapter One of 6 metal probes installed on the pipettor head.

During operation of the EZ1 Advanced, the tip adapters
pick up filter-tips from the worktable.

Tip holder A polypropylene tube that holds a single filter-tip. Tip

holders are loaded onto the tip rack.

Tip rack A metal rack that accommodates tip holders containing

filter-tips on the worktable. The tip rack also
accommodates sample tubes and elution tubes.

Tray A metal tray that is located under the worktable. It

collects any drops of liquid that may fall.

UV lamp A light source of ultraviolet light for decontamination.

VFD Vacuum fluorescence display, a message-display screen

using vacuum-tube technology.

Worktable The surface of the EZ1 Advanced that contains racks and
is where samples, reagent cartridges, and disposable
labware are loaded. The worktable moves backwards
and forwards to present different samples and reagents
under the pipettor head.

EZ1 Advanced User Manual 05/2008 8-3

Glossary

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8-4 EZ1 Advanced User Manual 05/2008

 Appendix A

Appendix A
Technical data
QIAGEN reserves the right to change specifications at any time.

Environmental conditions
Operating conditions

Power 100–120 V AC, 50/60 Hz, 240 VA (North America and

Japan)
200–240 V AC, 50/60 Hz, 240 VA (Europe)
Mains supply voltage fluctuations are not to exceed 10%
of nominal supply voltages.

Fuses 6.3 A (250 V) time-delay fuse (for 100–120 V AC)

3.15 A (250 V) time-delay fuse (for 200–240 V AC)

WARNING Electrical hazard [W13]

Never install a fuse different from that specified in the
user manual.

Overvoltage II
category

Air temperature 5–40ºC (41–104ºF)

Relative humidity 15–75% (noncondensing)

Maximum 75% relative humidity for temperatures up to
31ºC (88ºF), decreasing linearly to 50% humidity at
40°C (104°F)

Altitude Up to 2000 m (6500 ft.)

EZ1 Advanced User Manual 05/2008 A-1

Appendix A

Place of For indoor use only

operation

Pollution level 2

Environmental 3K2 (IEC 60721-3-3)

class

Transportation conditions

Air temperature –25ºC to 60ºC (–13ºF to 140ºF) in manufacturer’s

package

Relative humidity Minimum 15% to maximum 75% (noncondensing)

Environmental 2K2 & 2M2 (IEC 60721-3-2)

class

Storage conditions

Air temperature 5ºC to 40ºC (41ºF to 104ºF) if in manufacturer’s

package

Relative humidity Minimum 15% to maximum 75% (noncondensing)

Environmental 1K2 & 1M2 (IEC 60721-3-1)

class

A-2 EZ1 Advanced User Manual 05/2008

 Appendix A

Mechanical data and hardware features

Dimensions Width: 0.33 m (13 in.)

Height: 0.57 m (22.5 in.)
Depth: 0.51 m (20 in.)
0.57m (22.5 in.) including power connector

Mass 29 kg (64 lb.)

Instrument „ Automated nucleic acid isolation using magnetic

features particles
„ Desktop instrument
„ Protocols stored on EZ1 Advanced Cards
„ To be used only with QIAGEN EZ1 Kits
„ Aspirates and dispenses 6 samples or reagents
simultaneously using a 6-channel pipettor head
„ Separates magnetic particles using patented
technology
„ Processes up to 6 samples in one run
„ Controlled through VFD user interface
„ Temperature control through a heating system

Data tracking Bar code reader and manual keyboard enable data
tracking of samples and consumables. System and run
parameters are stored in a report file that can be directly
printed or transferred to a PC. Report files can be
processed with a LIMS (Laboratory Information
Management System) or other programs.

EZ1 Advanced User Manual 05/2008 A-3

Appendix A

Pipettor head Contains 6 high-precision syringe pumps, each containing

a tip adapter that attaches to filter-tips. Each tip aspirates
and dispenses 50–1000 μl liquid.
Pipetting accuracy is as follows:
„ 50–100 μl: ± 5%
„ 100–1000 μl: ± 2%
Syringe pumps are air-filled.
Liquids containing salts, alcohol, solvents, and/or
magnetic particles can be aspirated and dispensed.
Air gaps can be aspirated to prevent aspirated liquid from
dripping.
The pipettor head contains a magnet that allows
separation of magnetic particles from the aspirated liquid.
Filter-tips are picked up from the tip rack and ejected back
into the tip rack.
The pipettor head moves in the Z direction above the
worktable.

Heating system Accommodates the heating positions of reagent cartridges

and has a temperature range of between ambient
temperature and 95ºC (203ºF).
Heating block accuracy at 60°C is ± 2°C.

Filter-tips Attach to the tip adapters of the pipettor head to allow

liquid aspiration and dispensing. Capacity of 50–1000 μl.
The EZ1 Advanced accommodates up to 12 tip holders,
each containing a filter-tip, in the tip rack on the
worktable.

A-4 EZ1 Advanced User Manual 05/2008

 Appendix A

Labware Reagents are loaded onto the worktable using the reagent
cartridges. These cartridges are already prefilled with
reagents by QIAGEN.
Up to 6 reagent cartridges are accommodated together on
the worktable by a cartridge rack.
Samples are loaded onto the worktable using 2 ml sample
tubes.
Steps that require heating occur on the heating system,
which accommodates the heating positions of the reagent
cartridges.
Purified nucleic acids are collected in 1.5 ml elution tubes.

UV lamp Produces UV light with wavelength of 253.7 nm,

corresponding to ultraviolet light type C.

Capacity Up to 6 samples per run

EZ1 Advanced User Manual 05/2008 A-5

Appendix A

Waste Electrical and Electronic Equipment

(WEEE)
This section provides information about disposal of waste
electrical and electronic equipment by users in the European
Union.
The European Directive 2002/96/EC on WEEE requires
proper disposal of electrical and electronic equipment when
it reaches its end of life. The crossed-out wheeled bin symbol
(see below) indicates that this product must not be disposed
of with other waste; it must be taken to an approved
treatment facility or to a designated collection point for
recycling, according to local legislation. The separate
collection and recycling of waste electronic equipment at the
time of disposal helps to conserve natural resources and
ensures that the product is recycled in a manner that protects
human health and the environment.

QIAGEN accepts its responsibility in accordance with the

specific WEEE recycling requirements and, where a
replacement product is being supplied by QIAGEN, provides
free recycling of its WEEE-marked electronic equipment in
Europe. If a replacement product is not being purchased
from QIAGEN, recycling can be provided upon request at
additional cost. To recycle electronic equipment, contact your
local QIAGEN sales office for the required return form. Once
the form is submitted, you will be contacted by QIAGEN
either to request follow-up information for scheduling
collection of the electronic waste or to provide you with an
individual quote.

A-6 EZ1 Advanced User Manual 05/2008

 Appendix A

EC declaration of conformity
Name and address of the company
QIAGEN GmbH
QIAGEN Strasse 1
40724 Hilden
Germany
We herewith declare that the product

EZ1 Advanced, cat. no. 9001410 – 11

meets all applicable requirements of the following European

Directives

Low Voltage Directive (LVD) 2006/95/EC

Electromagnetic Compatibility 2004/108/EC

Directive (EMC)

In vitro diagnostic medical devices 98/79/EEC

(IVD)*

Hombrechtikon, 10 April 2008

Pit Muggli
Director Business Excellence

QIAGEN Instruments AG

* Only in combination with dedicated EZ1 DSP Kits.

EZ1 Advanced User Manual 05/2008 A-7

Appendix A

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A-8 EZ1 Advanced User Manual 05/2008

 Appendix B

Appendix B
Liability clause
QIAGEN shall be released from all obligations under its
warranty in the event repairs or modifications are made by
persons other than its own personnel, except in cases where
the Company has given its written consent to perform such
repairs or modifications.
All materials replaced under this warranty will be warranted
only for the duration of the original warranty period, and in
no case beyond the original expiration date of original
warranty unless authorized in writing by an officer of the
Company. Read-out devices, interfacing devices and
associated software will be warranted only for the period
offered by the original manufacturer of these products.
Representations and warranties made by any person,
including representatives of QIAGEN, which are inconsistent
or in conflict with the conditions in this warranty shall not be
binding upon the Company unless produced in writing and
approved by an officer of QIAGEN.

EZ1 Advanced User Manual 05/2008 B-1

Appendix B

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B-2 EZ1 Advanced User Manual 05/2008

 Index

Index
Maintenance, 6-1
B daily, 6-6
decontamination, 6-8
Bar code reader, 5-29 weekly, 6-7
installing, 4-10 Manual operation, 5-17
clean, 5-18
C home axis, 5-17
resend, 5-19
Cartridge rack, 3-9 return tip, 5-18
Connector panel, 3-5
Control panel, 3-1, 3-2
O
D Operating conditions, 1-4, A-1

Door, 3-1, 3-2

closing, 5-6 P
opening, 5-5 PC software, 5-36
installing, 4-11, 4-17
E user interface, 5-36
Piercing unit, 3-11
Elution tubes, 3-7 Pipettor head, 3-1, 3-11
loading, 5-11 Power cord, 3-6
EZ1 Advanced Card, 3-1 Power requirements, 4-1
inserting, 5-2 Power switch, 3-6
removing, 5-4 Printer, 5-30
slot, 3-2, 3-4 installing, 4-10
Protocol, 3-1
F starting and finishing, 5-7
stopping, 5-8
Filter-tips, 3-7
loading, 5-11
Fuse box, 3-6, 4-2 R
Racks, 3-6
G Reagent cartridges, 3-1, 3-9
loading, 5-10
Glossary, 8-1 Reagent wells, 3-10
Report file, 5-32
H
Heating positions, 3-10 S
Heating system, 3-1, 3-10 Safety
biological, 1-4
M chemical, 1-6
electrical, 1-3
Magnet, 3-11
heat hazard, 1-7
Magnetic particles, 3-1

EZ1 Advanced User Manual 05/2008 Index-1

Index

mechanical hazards, 1-7 Tip holders, 3-1, 3-7

proper use, 1-2 Tip rack, 3-7
symbols, 1-8 Tips, 3-7
Sample tubes, 3-7 loading, 5-11
loading, 5-11 Transportation conditions, A-2
Servicing, 6-2 Tray, 3-10
Status LEDs, 3-2, 3-5 removing, 5-9
Storage conditions, A-2 Troubleshooting, 7-1
Switching off, 5-5 door, 7-1
Switching on, 5-5 error codes, 7-3
Symbols, 1-8, 1-9
Syringe pumps, 3-11 U
System setup, 5-26
date, 5-26 UV lamp, 3-12, 5-13, 5-16
preventive maintenance reminder, switching off, 5-15
5-29 switching on, 5-14
serial port, 5-28
time, 5-27 V
VFD, 3-2, 3-3
T
Test, 5-20 W
axis, 5-21
heating block, 5-22 Waste disposal, 1-6, A-6
serial port, 5-23 Worktable, 3-1, 3-6
version, 5-26 setting up, 5-9
Tip adapters, 3-11

Index-2 EZ1 Advanced User Manual 05/2008

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