11 SENIORHIGH

DAVAO INSTITUTE OF TECHNICAL EDUCATION, INC

EARTH AND LIFE SCIENCE

QUARTER 2
Module 4
GENETIC ENGINEERING

Name:

Grade & Section:

 MODULE 4: Genetic Engineering

Since ancient times the practice of genetic engineering had begun. Artificial selection is
done to indirectly manipulate genes focusing on the physical traits among organisms. Breeders
choose which organism to mate and produce offspring with desirable traits. They maintain this
procedure without control of what genes can be passed. Selective breeding is a process when
animals with desired characteristics are mated to produce offspring with those desired traits such
as Angus cows are bred to increase more meat. Hybridizations are when two individuals with unlike
characteristics are crossed to produce the best in both organisms like the disease resistant potato
called the Burbank potato. Inbreeding is a technique of breeding organisms that are genetically
similar to maintain desired traits found in the pure dog breeds. As defined, genetic engineering is
the process of changing the DNA in living organisms to create something new. It involves artificial
manipulation, modification, and recombination of DNA or other nucleic acid molecules to modify an
organism or population of organisms.

Recombinant DNA Technology

Recombinant DNA is made by mixing DNA from two different sources. Restriction enzymes
were discovered in 1968 by Swiss microbiologist Werner Arbe. This was used to splice, connect (or
ligate), and remove or add nucleotides to sequences of the DNA. This process is used in
recombinant DNA technology to remove and insert genetic sequences from and into other
sequences of another organism. Inserting the desired gene into the genome of the host involves
the selection of the desired gene for administration into the host followed by a selection of the
perfect vector (either plasmid or bacteriophage) with which the gene has to be integrated, thus
recombinant DNA is formed. The recombinant DNA has to be introduced into the host, maintained
and carried forward to the offspring. Biotechnology and pharmaceutical companies practice this
technique in working with medical and research purposes.

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DAVAO INSTITUTE OF TECHNICAL EDUCATION, INC

Earth and Life Science
LEARNING ACTIVITY SHEET

Name: ___________________________________________________ Module #: _____________

Grade and Section: _________________________________________ Score: _______________

Quarter 2 - Module 4
Activity 1
Match me! Using the terms in the box, match the genetic techniques in their descriptions. Write your answer in
the space given.

________________1. A science of changing the DNA in living organisms to create something new
________________2. Breeders choose which organism to mate to produce offspring with desired traits.
________________3. Animals with desired characteristics are mated to produce offspring with those desired traits.
________________4. Two individuals with unlike characteristics are crossed to produce the best in both organisms.
________________5. Breeding of organism that genetically similar to maintain desired traits.
________________6. Creating an organism that is an exact genetic copy of another
________________7. DNA is cut out of one organism and put into another organism
________________8. A technique used to compare DNA from two or more organisms

Activity 2
Genetic Engineering Techniques
Directions. Distinguish the techniques in genetic engineering based on the situation and examples
given. Write the letter of the choices.

____1. When animals with desired characteristics are mated to produce offspring with those desired traits. Dachshund were once
bred to hunt badgers and other burrowing animals.
____2. Creating an organism that is an exact genetic copy of another. They will have the same exact DNA as the parent.
____3. DNA is cut out of one organism and put into another organism. A trait will be transferred from one organism to another.
____4. Luther Burbank created a disease resistant potato called the Burbank potato. He crossed a disease resistant plant with one
that had a large food producing capacity.
____5. A technique used to compare DNA from two or more organisms.
____6. Breeding of organisms that are genetically similar to maintain desired traits. It keeps each breed unique from others.

Activity 3
Genetic Engineering Techniques
Directions. Express your idea, whether you agree or disagree in the following statements about the current technology in
genetic engineering. Write your answers in your journal notebook. Choose two from the three techniques.

1. Monkeys have been Cloned, Paving the way for Human Cloning
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2. Bacteria as Living Microrobots to Fight Cancer
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3. Two genetic regions (variants in both ABO blood group locus and cluster of genes in chromosome 3) Linked with Severe
COVID-19
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EARTH AND LIFE SCIENCE | PAGE 2

____________________________________________________

DAVAO INSTITUTE OF TECHNICAL EDUCATION, INC

Earth and Life Science
LEARNING ACTIVITY SHEET

Name: ___________________________________________________ Module #: _____________

Grade and Section: _________________________________________ Score: _______________

Quarter 2 - Module 4

Multiple Choice. Choose the letter of the best answer. Write the chosen letter on the space provided.

______ 1. It is the process of manipulating genes in order to have the desired trait.
A. artificial selection C. gene splicing
B. cloning D. selective breeding
______ 2. It is the process of breeding an organism that is genetically similar to maintain desired traits. It keeps each
breed unique from others.
A. artificial selection C. inbreeding
B. cloning D. selective breeding
______ 3. It is a technique where the DNA is cut out of one organism and put into another organism. A trait will be
transferred from one organism to another.
A. artificial selection C. gene splicing
B. cloning D. gel electrophoresis
______ 4. It is a technique of creating an organism that is an exact genetic copy of the other organism.
A. artificial selection C. gene splicing
B. cloning D. gel electrophoresis
______ 5. An applied science of manipulating the genetic material of an organism through artificial selection and
biomedical techniques
A. artificial selection C. genetic engineering
B. cloning D. selective breeding
______ 6. It refers to the joining of almost any nucleotide sequence to create a new gene product or to introduce a new
gene sequence.
A. DNA Recombinant technology C. both a and b
B. Recombinant DNA technology D. none of a and b
______ 7. It is produced chiefly by certain bacteria and has the property of cleaving DNA molecules at or near a
specific sequence of bases.
A. bacteriophage enzymes C. restriction enzymes
B. plasmid enzymes D. vector enzymes
______ 8. Most common vectors used in genetic engineering techniques
A. bacteriophage and plasmid C. plasmid and DNA
B. enzymes and plasmid D. restriction enzymes and DNA
______ 9. Which is not performed to produce recombinant DNA?
A. Insertion of the genetic sequences into other sequences of another organism.
B. Removal of the genetic sequences of an organism.
C. Selection of the perfect vector with which the gene must be integrated
D. Selection of the perfect enzymes with which the gene must be integrated.
______ 10. Which is not a tool in recombinant DNA technology?
A. enzymes C. restriction enzymes
B. host organisms D. vectors
______ 11. Which is not an ancient practice of genetic engineering?
A. hybridization C. recombinant technology
B. inbreeding D. selective breeding
______ 12. Two individuals with unlike characteristics are crossed to produce the best in both organisms.
A. artificial selection C. genetic engineering
B. cloning D. hybridization
______ 13. In recombinant DNA technology which is most used as a host organism?
A. bacteria C. plasmid
B. cells D. vectors
For numbers 14-15, refer to the following choices below.

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A. Statement I is correct B. Statement II is correct.
C. Statement I and II are both correct. D. Statements I and II are both incorrect.
______ 14. I - Since ancient times the practice of genetic engineering had begun.
II - Artificial selection is done to directly manipulate genes focusing on the physical traits among
organisms.
______ 15. I – Enzymes help in Recombinant DNA technology by carrying and integrating the desired gene,
usually plasmids and bacteriophages.
II - Host organisms are used to propagate the recombinant DNA.

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