Group 3 - Poster (Prelims)

Acaricidal Activity of Madre Cacao (Gliricidia sepium) and Gabi (Colocasia
esculenta) Leaves Extract Against Dog Ticks (Rhipicephalus sanguineus)

Bayan, A.M.L., De Guzman, Z.C.O., Del Rosario, M.J.O., Espejo, J.A.L.,
Guarino, R.U., Laigue, M.C., Talavera, L.M.G.
Research Adviser: Arenz Maryan M. Ambatali, RPh
College of Pharmacy, PHINMA-Araullo University
ABSTRACT INTRODUCTION
Undoubtedly, one of the major challenges that a country may face is a Ticks are one of the biggest public health and veterinary problems in
health-related problem, of which veterinary health is one. Ticks affect not the world. It affects not only the animals infested but also the human
only animals but also the human health and the economy. To address the health and the economy. Dogs are known to be often infested by ticks
problem, the researchers come up with the idea to find a solution in generally because they are often staying outside. A study conducted by
eliminating ticks using Madre cacao and Gabi leaves extract. To test its Morgan, L., et al., 2020, stated that owning pets, such as dogs, has
effectivity, this study employed quantitative - experimental research design
potential benefits to humans such as keeping their mental health in a
as the most appropriate approach to the problem. The pharmacologic
good state. But owning a pet should not be only beneficial to humans.
treatments were conducted in PHNMA - Araullo University. Results of the
People shall also give their best effort to provide enough of its needs.
treatments revealed that Madre cacao leaves had an average mortality rate
of 80% for three trials, Gabi leaves at 93.33%, and the combination showed Getting rid of ticks is one of the most important things to check on dogs as
100% mortality rate. Results were analyzed using Analysis of Variance to ticks may cause on the emergence of tick-borne diseases.
test its differences. Findings revealed that the effects of the treatments were
To address the problem, the researchers came up with the idea to find
not significantly different to each other, but are significantly different on the
negative control and the positive control. a solution to eliminate ticks on dogs by using madre cacao leaves and
gabi leaves extract. Thus, this study was conducted to determine the
Therefore, the researchers concluded that Madre cacao leaves extract acaricidal activity of madre cacao (Gliricidia sepium) leaves extract and
and Gabi leaves extract had an acaricidal activity against dog ticks. This gabi (Colocasia esculenta) leaves extract against dog ticks (Rhipicephalus
study can have a potential impact in the field of pharmacy and in the human sanguineus).
to help the environment in the possible threat of tick-borne diseases.
OBJECTIVES
GENERAL OBJECTIVES 2. What are the differences between the mortality rate of dog ticks using the
This study aimed to determine the acaricidal activity of Madre Cacao following:
(Gliricidia sepium) and Gabi (Colocasia esculenta) leaves extract against 2.1 Madre cacao leaves extract
dog ticks (Rhipicephalus sanguineus) 2.2 Tanglad leaves extract
2.3 Madre cacao and tanglad leaves extract
SPECIFIC OBJECTIVES
3. What are the differences between the effects on mortality rate of dog ticks
1. How effective are madre cacao leaves extract shampoo, gabi leaves
using the madre cacao leaves, gabi leaves extract and it's combination to the
extract shampoo, and madre cacao and gabi leaves extract as acaricide
following:
against brown dog ticks in terms of:
3.1 Negative Control: Water
1.1 Number of Dead Ticks after 10 minutes.
3.2 Positive Control: Kennels Best Madre de Cacao Shampoo
1.2 Number of Alive Ticks within 10 minutes.
4. What are the components that is present in Madre Cacao and gabi leaves
1.3 Percentage of Number of Dead Ticks after 10 minutes.
extract that contributes to its acaricidal activity against dog ticks?
METHODOLOGY SUMMARY OF FINDINGS

Quantitative- Pharmacy
Experimental Laboratory, Pharmacologic
Research PHINMA-Araullo Treatment
Design University
RESEARCH DESIGN LOCALE OF THE STUDY SOURCES OF DATA
Collection of Madre Phytochemical screening
Cacao and Gabi Collection of Dog Ticks
Percentage
Leaves Pharmacologic treatment Analysis of
Extraction Statistical analysis Variance
Concentration
DATE GATHERING DATA ANALYSIS Comparison among means of mortality rate of
PROCWDURE Rhicephalus sanguineus among tretaments
RESULTS AND DISCUSSION

CONCLUSIONS
One-way Analysis of Variance (ANOVA) was performed to determine the
1. There is no significant difference between the effects on
effects of three different treatments to the mortality rate of dog ticks
mortality rate dog ticks using madre cacao leaves extract, gabi
(Rhipicephalus sanguineus). The results showed that the value of p using the
leaves extract and its combination.
ANOVA is 0.0001, F (4,14)=33.36. Since the p value is less than the
2. There is a significant difference between the effects on
significance level, 0.05, the null hypothesis was rejected.
mortality rate of dog ticks using the madre cacao leaves
Thus, the effect of three treatments using the extracts were significantly
extract, gabi leaves extract and its combination to water and
different to the effect of the water and the commercial shampoo.
the commercial shampoo
Therefore, the researchers concluded that madre cacao and gabi leaves
extract had an acaricidal activity against dog ticks. Enriquez O., et al., (2019). The Effectiveness of Kakawate
(Gliricidia sepium) Extract as an Alternative to Inorganic
Dog Shampoo. Retrieved
RECOMMENDATIONS
from https://www.scribd.com/document/436743470/Kakawa
REFERENCES
te-Dog-Soap-
Thesis?fbclid=IwAR0wwiRnjmkV3HKgOgyUZu0jPK81bkiQ
Different kinds of skin testing using the extracts are recommended.
mj2kt6pIQd7KNMh6Y0siPK_6i8I.
The future researchers must also consider Isolation of the chemical constituent that Olmeda, S. et al., (2008), Toxicity of oxalic acid against
formulating a shampoo using the extract. contributes to its acaricidal activity must also be adult Hyalomma lusitanicum ticks (Ixodida: Ixodidae) in
laboratory conditions: LD50. National Library of Medicine.
considered
45(4):715-9

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Acaricidal Activity of Madre Cacao (Gliricidia sepium) and Gabi (Colocasia

esculenta) Leaves Extract Against Dog Ticks (Rhipicephalus sanguineus)

METHODOLOGY SUMMARY OF FINDINGS

RESULTS AND DISCUSSION

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