Week 4 - Shifting Exam: A Thick Polysaccharide Capsule
Week 4 - Shifting Exam: A Thick Polysaccharide Capsule
✓ 1. All of the following are virulence factors associated with N gonorrhoeae except
Points: Pili
1/1 Por
A thick polysaccharide capsule ✓
Lipooligosaccharide
✘ 2. When staining acid-fast bacilli with Truant auramine-rhodamine stain, potassium permanganate is used as a:
Points: Gallbladder ✓
0/1 Kidney
Liver
Intestines ✘
✘ 4. A sputum specimen received at 8 AM for an AFB smear reveals acid- fast bacilli. An additional sputum is submitted that
afternoon. This specimen was concentrated by the NALC-sodium hydroxide method and inoculated on 2 Lowenstein-Jensen slants
Points: and held for 8 weeks at 35°C in 5%-10% CO₂. No growth occurs. The best explanation is that:
0/1
an improper specimen was submitted for culture ✓
cultures were held for an insufficient period of time
improper media was used for culture
the hypochlorite technique was not used ✘
Points: PAD ✘
0/1 LIA
TSI
Indole ✓
✓ 6. An autopsy performed on an 8-year-old child revealed Waterhouse-Friderichsen syndrome. Blood and throat cultures taken just
prior to death were positive for which organism?
Points:
1/1 Neisseria meningitidis ✓
Haemophilus influenzae
Neisseria gonorrhoeae
Klebsiella pneumoniae
✓ 7. 1. Identify the organism.
Points: Culture : MAC – colonies are clear and colorless (NLF)
1/1
Urease : +
Gelatinase : +
ONPG : +
Arabinose fermentation : -
TSI : K/A, gas (+), H2S (-)
Serratia marcescens ✓
Klebsiella pneumoniae subsp. pneumoniae
Pantoea agglomerans
Escherichia coli
✘ 8. They are plump coccobacilli that tend to resist alcohol decolorization, they may be mistaken for Neisseria spp. for being oxidase
positive.
Points:
0/1 Acinetobacter ✓
Moraxella ✘
Enterobacter
Stenotrophomonas
✓ 9. It the most virulent specie that causes bacillary dysentery and has shiga toxin as a virulence factor. Identify the organism using
the following laboratory results.
Points:
1/1 Urease : -
LDC : -
IMViC : v+--
TSI : K/A, gas (-), H2S (-)
S. flexneri
S. boydii
S. sonnei
S. dysenteriae ✓
✓ 10. M. morganii and P. vulgaris have the same biochemical reaction, what is the possible test used to differentiate these species?
Points: Citrate ✓
1/1 Indole
PAD
Urease
✘ 13. A 10-year-old child has a primary pulmonary Mycobacterium tuberculosis infection. Which of the following features of
tuberculosis is most correct?
Points:
0/1 If tuberculosis develops years later, it is a result of another exposure to M tuberculosis. ✘
In primary tuberculosis, all of the infecting M tuberculosis organisms are killed by the patient’s immune response.
In primary tuberculosis, an active exudative lesion develops and rapidly spreads to lymphatics and regional lymph nodes.
✓
The exudative lesion of primary tuberculosis often heals slowly.
✓ 15. The MTOD noticed that there is a green discoloration around the colonies that grew in CHOC. How will he proceed?
✘ 17. An aspirated specimen of purulent material was obtained from a brain abscess. After 24 hours incubation, pinpoint colonies
grew on sheep blood and small, yellowish colonies grew on chocolate. Gram stain of the organism showed gram-negative cocci.
Points: Results of carbohydrate degradation studies were as follows:
0/1
glucosa:acid
maltose:acid
sucrosa:acid
Lactoaa:negative
Additional testing revealed that the organism was oxidase positive and beta-galactosidase negative. The organism is most likely
Neisseria:
N. lactamica ✘
N. gonorrhoeae
N. sicca ✓
N. meningitidis
✓ 18. The diagnosis of Neisseria gonorrhoeae in females is best made from:
✘ 19. This test result dictates the algorithm to be followed if the isolate is catalase-negative.
Points: -+-+ ✓
0/1 ++-- ✘
+-+-
--++
✓ 21. TRUE OR FALSE. Tube coagulase test is preferable than slide coagulase test.
Points: FALSE, because it consumes more reagent, and needs more time before results can be interpreted
1/1 TRUE, because it is more specific than slide coagulase test ✓
FALSE, because it is not specific nor sensitive
TRUE, because it is more sensitive than slide coagulase test
✓ 22. A 31-year-old Asian woman is admitted to the hospital with a 7-week history of increasing malaise, myalgia, nonproductive
cough, and shortness of breath. She has daily fevers of 38–39°C and a recent 5-kg weight loss. She had a negative chest
Points: radiograph when she entered the United States 7 years ago. The patient’s grandmother died of tuberculosis when the patient was
1/1 an infant. A current chest radiograph is normal; results of other tests show a decreased hematocrit and liver function test
abnormalities. Liver and bone marrow biopsies show granulomas with giant cells and acid-fast bacilli. She is probably infected with:
Mycobacterium gordonae
Mycobacterium tuberculosis ✓
Mycobacterium ulcerans
Mycobacterium fortuitum
C. freundii ✓
C. diversus
C. koseri
M. morganii
✘ 24. All of the following are components of LIA, except.
Points: Ammonium ✓
0/1 Peptones
Lysine
0.1% Glucose ✘
✓ 27. Which of the following cell components produced by Neisseria gonorrhoeae is responsible for attachment to host cells?
✘ 30. TRUE OR FALSE. Slide catalase test is preferable than tube catalase test.
✓ 32. The MTOD performed the algorithm for gram-positive cocci and one of the test results is a jelly-like substance from defibrinated
plasma. What test is being interpreted?
Points:
1/1 slide coagulase test
catalase test
PYR test
tube coagulase test ✓
✓ 33. This test determines the organism’s ability to produce keratoconjunctivitis in guinea pigs. This is also a virulence test
for Shigella and EIEC.
Points:
1/1 String Test
Neufeld-Quellung Test
Sereny Test ✓
4-methylumbelliferyl-B-D-glucuronide
✓ 34. Which M. avium complex (MAC) organism is the most frequently isolated mycobacterium from AIDS patients?
Points: M. intracellulare
1/1 M. bovis
M. scrofulaceum
M. avium ✓
✘ 35. This organism has a sensitive result when tested with Vibriostatic test O/129.
✘ 37. It is the hallmark of enteric fever infection during the second week of fever.
✓ 40. The function of N-acetyl-L-cysteine in the reagent for acid-fast digestion-decontamination procedure is to:
✓ 41. 1. A child was rushed into the hospital due to malaise, anorexia, myalgia and severe frontal headache, the doctor suspected
Points: that the child have enteric fever. The laboratory tests were requested and these are the following results.
1/1 Laboratory Results:
Culture : MAC – colonies are clear and colorless (NLF)
SSA – colonies are colorless with black centers
Urease : -
IMViC : -+--
TSI : K/A, gas (-), H2S (+)
Points: I. In a K/A reaction in TSI, this means that the glucose is depleted.
1/1 II. In an A/A reaction in TSI, this means that the enterobacteria attacks the lactose and sucrose first and then the glucose.
Both statements are true
First statement is true ; Second statement is false ✓
First statement is false ; Second statement is true
Both statements are false
✓ 43. A 19-year-old man presented to the clinic with a urethral discharge for the past 24 hours. Neisseria gonorrhoeae was cultured
from the specimen and found to be β-lactamase positive and resistant to high levels (≥32 μg/mL) of tetracycline. Which of the
Points: following statements about these antimicrobial resistance factors is correct?
1/1
Whereas β-lactamase production is mediated by a gene on the bacterial chromosome, high-level tetracycline
resistance is mediated by a gene on a plasmid.
✓
β-lactamase production and high-level resistance to tetracycline are both mediated by genes on the bacterial chromosome.
β-lactamase production and high-level resistance to tetra-cycline are both mediated by genes on plasmids.
Whereas β-lactamase production is mediated by a gene on a plasmid, high-level tetracycline resistance is mediated by a
gene on the bacterial chromosome.
✘ 46. Which of the statements is true about the Kligler Iron Agar?
Points: I. It has the same components as those of TSI except for glucose.
0/1 II. It contains ten times more sucrose than glucose as compared with TSI.
I only
II only
None of the choices ✓
I and II ✘
Points: M. tuberculosis
1/1 M. marinum
M. ulcerans ✓
M. chelonei
✘ 49. An 18-year-old woman who reports unprotected sex with a new partner 2 weeks previously develops fever and left lower
quadrant abdominal pain with onset in association with her menstrual period. On pelvic examination in the emergency department,
Points: she has bilateral tenderness when the uterus is palpated. A mass 2–3 cm in diameter is felt on the left, suggestive of tubo-ovarian
0/1 abscess. Subsequently, Neisseria gonorrhoeae is cultured from her endocervix. The diagnosis is gonococcal pelvic inflammatory
disease. A common sequela of this infection is
Urethral stricture
Uterine fibroid tumors ✘
Infertility ✓
Cancer of the cervix
✓ 50. It is very important that a patient diagnosed with tuberculosis can also be evaluated for
Points: HIV/AIDS ✓
1/1 Liver abscess
Lymphoma
Typhoid fever
✘ 51. What organism produces susceptibility or any zone of inhibition to Taxo A test?
✓ 52. It is a heme enzyme that catalyzes the decomposition of H2O2 to water and oxygen, and its positive reaction.
✘ 54. A physician examined a patient's wound and noticed that it is a black, leathery tissue with foul smelling discharge. Direct C/S
reveals gram-positive cocci is present on the wound. The most likely causative agent is resistant to ____________.
Points:
0/1 NONE OF THESE
ALL OF THESE
taxo P ✓
taxo A ✘
✘ 55. You observe a 40-year-old man begging on a street in a town in India. He has clawing of the fourth and fifth digits with loss of
distal parts of the digits of both hands, strongly suggesting leprosy. The causative agent of this disease
Points:
0/1 Grows in parts of the body that are cooler than 37°C ✓
Is susceptible to isoniazid and rifampin
Can be cultured in the laboratory using Middlebrook 7h11 Medium ✘
Is seen in high numbers in biopsies of tuberculoid leprosy lesions
✓ 56. A 47-year-old woman presents with a 3-month history of progressive cough, weight loss, and fever. Chest radiography shows
bilateral cavitary disease suggestive of tuberculosis. Sputum culture grows an acid-fast bacillus that is a photochromogen (makes
Points: an orange pigment when exposed to light). The organism most likely is
1/1
Mycobacterium tuberculosis
Mycobacterium kansasii ✓
Mycobacterium avium complex
Mycobacterium gordonae
✘ 58. A useful test to differentiate Moraxella catarrhalis from saprophytic neisseriae in respiratory samples is
Points: I. Citrate Utilization Test is used to determine the ability of the organism to utilize sodium citrate as its only carbon source
1/1 and inorganic ammonium salts as its only nitrogen source.
II. If citrate is used as a carbon source, the medium will turn alkaline.
First statement is true ; Second statement is false
Both statements are false
Both statements are true ✓
First statement is false ; Second statement is true
Y. pestis ✓
Y. enterocolitica
Plesiomonas
Y. pseudotuberculosis
✘ 63. A vaginal smear is submitted for Gram stain for Neisseria gonorrhoeae. The technologist finds the ONLY following results on
the Gram stain:
Points:
0/1 many white blood cells
few epithelial cells
many gram-positive bacilli
few gram-negative diplococci
few gram-positive cocci in chains
The technologist should:
do not read or report a Gram stain on a vaginal specimen ✓
report out smear positive for gonorrhea ✘
report out smear negative for gonorrhea
request a new specimen due to number of white blood cells
✘ 66. A 33 year old man comes to the ER complaining about his cola-colored urine so the physician orders a urinalysis, and some
other tests. The MTOD in Clinical Microscopy observes that the urine sample has many RBC casts, dysmorphic RBCs, and
Points: increased protein so he releases the results. When the physician sees all the results, she concludes that the patient is suffering
0/1 from Post-streptococcal acute glomerulonephritis. What is the most deciding factor of the physician for her final diagnosis?
her gut instincts
px medical history
other tests (e.g. ASO titer) ✓
urinalysis result ✘
✓ 67. According to Bergey's Academic classification, Viridans streptococcus can grow at what temperature?
✘ 68. A first morning sputum is received for culture of mycobacteria. It is digested and concentrated by the N-acetyl-L-cysteine alkali
method. Two Lowenstein-Jensen slants are incubated in the dark at 35°C with 5%-10% CO, The smears reveal acid-fast bacilli,
Points: and after 7 days no growth appears onthe slants. The best explanation is:
0/1
exposure to CO₂ prevents growth
improper specimen submitted
incorrect concentration procedure ✘
cultures held for insufficient length of time ✓
Points: TSI
0/1 Urease ✓
PAD ✘
Indole
✓ 70. The MTOD processed a urine sample and during direct gram-staining, he saw that the bacteria is gram+ spheres. There is
growth on BAP but no growth on MAC. How will the MTOD proceed?
Points:
1/1 look at the hemolytic pattern
do indirect gram staining
perform catalase test ✓
perform TSI innoculation
Points: 0% ✓
1/1 5%
0.5%
20%
Points: Y. pestis ✓
1/1 E. coli
S. typhi
S. dysenteriae
✓ 75. Which organism, associated with tuberculosis in cattle, causes tuberculosis in humans, especially in regions where dairy
farming is prevalent?
Points:
1/1 Mycobacterium avium–intracellulare complex
Mycobacterium kansasii
Mycobacterium marinum
Mycobacterium bovis ✓
✓ 76. Identify the organism.
✘ 78. In XLD agar, differentiate the growth produced by Salmonella and Shigella.
Points: Salmonella - colonies exhibit a pink to red color : Shigella - colonies exhibit a pink to red color with black centers ✓
0/1 Salmonella - colonies exhibit a orange to red color : Shigella - colonies exhibit a orange to red color with black centers
Salmonella - colonies exhibit a red to pink color : Shigella - colonies exhibit a red to pink color with black
centers
✘
Salmonella - colonies exhibit a white to blue color : Shigella - colonies exhibit a white to blue color with black centers
✓ 79. A 60-year-old-man has a 5-month history of progressive weakness and a weight loss of 13 kg along with intermittent fever,
chills, and a chronic cough productive of yellow sputum, occasionally streaked with blood. A sputum specimen is obtained, and
Points: numerous acid-fast bacteria are seen on the smear. Culture of the sputum is positive for Mycobacterium tuberculosis. Which
1/1 treatment regimen is most appropriate for initial therapy?
Isoniazid and rifampin
Sulfamethoxazole–trimethoprim and streptomycin
Isoniazid, cycloserine, and ciprofloxacin
Isoniazid, rifampin, pyrazinamide, and ethambutol ✓
✘ 80. The best specimen for recovery of the mycobacteria a sputum sample is:
✓ 81. Which of the following is the most reliable test to differentiate Neisseria lactamica from Neisseria meningitidis?
✘ 83. The β-galactosidase test aids in the identification of which Neisseria species?
Points: N. flavescens
0/1 N. lactamica ✓
N. gonorrhoeae
N. meningitidis ✘
Points: alpha
0/1 no hemolysis
not applicable ✓
beta ✘
Points: II. In the Philippines, Streptococcus agalactiae colonization of the vagina is routinely done to expecting mothers near birth.
0/1 Which of the following statement/s is/are not incorrect?
II
I
ALL OF THESE ✘
NONE OF THESE ✓
✘ 86. It is the only oxidase-positive member of Enterobacteriaceae and it has been associated with HIV-positive individuals with
inflammatory bowel disease.
Points:
0/1 Y. pseudotuberculosis
Y. enterocolitica ✘
Y. pestis
Plesiomonas ✓
✘ 87. TRUE OR FALSE. Streptococcus pyogenes exhibits positive reaction in CAMP test because it enhances the beta-hemolysis of
Staphyloccocus aureus.
Points:
0/1 FALSE, S. pyogenes does not exhibit positive reaction with S. aureus
FALSE, S. pyogenes is not CAMP test positive ✘
TRUE, because S. pyogenes has diffusible, extracellular protein ✓
TRUE, because S. pyogenes has diffusible, intracellular protein
Points: Glycerol
0/1 Glucose ✘
Fatty acids ✓
Casein Hydrokysate
Points: endocarditis
1/1 ALL OF THESE ✓
gingivitis
dental carries
✓ 93. What are the deciding factors that can affect the integrity of BAP during agar preparation?
✓ 94. A Gram stain of a urethral discharge from a man showing extracellular and intracellular gram-negative diplococci within
segmented neutrophils is a presumptive identification for:
Points:
1/1 Neisseria lactamica
Moraxella (Branhamella) catarrhalis
Neisseria meningitidis
Neisseria gonorrhoeae ✓
✓ 95. In TSI reaction, after completing 18-24 hours of incubation, the organism has utilized the peptones in the slant, which results in
the formation of alkaline products and the change of the slant color to ________.
Points:
1/1 Blue
Red ✓
Black
Yellow
Points: Edwardsiella
0/1 Serratia ✓
Yersinia
E.coli
✘ 98. It is an extracellular enzyme that causes clot formation when bacterial cells are incubated with plasma.
✓ 99. What is the desired specimen for Salmonella identification during the second to third week of infection?
Points: Urine
1/1 Stool ✓
Blood
CSF