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Week 4 - Shifting Exam: A Thick Polysaccharide Capsule

1. The document contains questions about microbiology topics including identification of bacteria based on laboratory results and descriptions of clinical presentations. 2. The questions cover topics such as virulence factors of Neisseria gonorrhoeae, acid-fast staining techniques, sites of infection for enteric fever organisms, and characteristics of Mycobacterium tuberculosis. 3. The document appears to be a quiz or exam covering identification of bacteria and basic microbiology concepts.
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0% found this document useful (0 votes)
58 views

Week 4 - Shifting Exam: A Thick Polysaccharide Capsule

1. The document contains questions about microbiology topics including identification of bacteria based on laboratory results and descriptions of clinical presentations. 2. The questions cover topics such as virulence factors of Neisseria gonorrhoeae, acid-fast staining techniques, sites of infection for enteric fever organisms, and characteristics of Mycobacterium tuberculosis. 3. The document appears to be a quiz or exam covering identification of bacteria and basic microbiology concepts.
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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WEEK 4 - SHIFTING EXAM

✓ 1. All of the following are virulence factors associated with N gonorrhoeae except

Points: Pili
1/1 Por
A thick polysaccharide capsule ✓
Lipooligosaccharide

✘ 2. When staining acid-fast bacilli with Truant auramine-rhodamine stain, potassium permanganate is used as a:

Points: Decolorizing agent


0/1 Dye ✘
Mordant
Quenching agent ✓

✘ 3. It is the site of long term carriage in patients with enteric fever.

Points: Gallbladder ✓
0/1 Kidney
Liver
Intestines ✘

✘ 4. A sputum specimen received at 8 AM for an AFB smear reveals acid- fast bacilli. An additional sputum is submitted that
afternoon. This specimen was concentrated by the NALC-sodium hydroxide method and inoculated on 2 Lowenstein-Jensen slants
Points: and held for 8 weeks at 35°C in 5%-10% CO₂. No growth occurs. The best explanation is that:
0/1
an improper specimen was submitted for culture ✓
cultures were held for an insufficient period of time
improper media was used for culture
the hypochlorite technique was not used ✘

✘ 5. It is a test used to differentiate P. mirabilis and P. vulgaris.

Points: PAD ✘
0/1 LIA
TSI
Indole ✓

✓ 6. An autopsy performed on an 8-year-old child revealed Waterhouse-Friderichsen syndrome. Blood and throat cultures taken just
prior to death were positive for which organism?
Points:
1/1 Neisseria meningitidis ✓
Haemophilus influenzae
Neisseria gonorrhoeae
Klebsiella pneumoniae
✓ 7. 1. Identify the organism.
Points: Culture : MAC – colonies are clear and colorless (NLF)
1/1
Urease : +
Gelatinase : +
ONPG : +
Arabinose fermentation : -
TSI : K/A, gas (+), H2S (-)

Serratia marcescens ✓
Klebsiella pneumoniae subsp. pneumoniae
Pantoea agglomerans
Escherichia coli

✘ 8. They are plump coccobacilli that tend to resist alcohol decolorization, they may be mistaken for Neisseria spp. for being oxidase
positive.
Points:
0/1 Acinetobacter ✓
Moraxella ✘
Enterobacter
Stenotrophomonas

✓ 9. It the most virulent specie that causes bacillary dysentery and has shiga toxin as a virulence factor. Identify the organism using
the following laboratory results.
Points:
1/1 Urease : -
LDC : -
IMViC : v+--
TSI : K/A, gas (-), H2S (-)
S. flexneri
S. boydii
S. sonnei
S. dysenteriae ✓

✓ 10. M. morganii and P. vulgaris have the same biochemical reaction, what is the possible test used to differentiate these species?

Points: Citrate ✓
1/1 Indole
PAD
Urease

✓ 11. Staphylococcus cause hypersensitivity reaction resulting to:

Points: bullous impetigo


1/1 Ritter's disease
toxic shock syndrome ✓
food poisoning
✓ 12. Which nonpathogenic Mycobacterium species is isolated most often from clinical specimens and is called the “tapwater
bacillus”?
Points:
1/1 M. avium–intracellulare complex
M. kansasii
M. gordonae ✓
M. leprae

✘ 13. A 10-year-old child has a primary pulmonary Mycobacterium tuberculosis infection. Which of the following features of
tuberculosis is most correct?
Points:
0/1 If tuberculosis develops years later, it is a result of another exposure to M tuberculosis. ✘
In primary tuberculosis, all of the infecting M tuberculosis organisms are killed by the patient’s immune response.
In primary tuberculosis, an active exudative lesion develops and rapidly spreads to lymphatics and regional lymph nodes.

The exudative lesion of primary tuberculosis often heals slowly.

✘ 14. The definition of extensively drug resistant (XDR) tuberculosis includes:

Points: All of the above ✓


0/1 Resistance to a fluoroquinolone
Resistance to capreomycin, amikacin or kanamycin
Resistance to isoniazid and rifampin ✘

✓ 15. The MTOD noticed that there is a green discoloration around the colonies that grew in CHOC. How will he proceed?

Points: NONE OF THESE ✓


1/1 Interpret that the discoloration is because of the innate pigment of the organism
Interpret that the organism is alpha-hemolytic
Prepare a new plate because that media is non-viable already

✘ 16. What is the positive result of urea hydrolysis test?


Points: Medium exhibits a yellow color ✘
0/1
Change in the color of the slant from orange to magenta ✓
Liquified gel
Exhibits a blue color at pH 7.6

✘ 17. An aspirated specimen of purulent material was obtained from a brain abscess. After 24 hours incubation, pinpoint colonies
grew on sheep blood and small, yellowish colonies grew on chocolate. Gram stain of the organism showed gram-negative cocci.
Points: Results of carbohydrate degradation studies were as follows:
0/1
glucosa:acid
maltose:acid
sucrosa:acid
Lactoaa:negative
Additional testing revealed that the organism was oxidase positive and beta-galactosidase negative. The organism is most likely
Neisseria:
N. lactamica ✘
N. gonorrhoeae
N. sicca ✓
N. meningitidis
✓ 18. The diagnosis of Neisseria gonorrhoeae in females is best made from:

Points: Gram stain of cervical secretions


1/1 clinical history
examination for clue cells
an endocervical culture ✓

✘ 19. This test result dictates the algorithm to be followed if the isolate is catalase-negative.

Points: growth on MAC


0/1 hemolysis on BAP ✓
Bacitracin susceptibility test ✘
Optochin susceptibility test

✘ 20. IMViC reaction of S. enterica.

Points: -+-+ ✓
0/1 ++-- ✘
+-+-
--++

✓ 21. TRUE OR FALSE. Tube coagulase test is preferable than slide coagulase test.

Points: FALSE, because it consumes more reagent, and needs more time before results can be interpreted
1/1 TRUE, because it is more specific than slide coagulase test ✓
FALSE, because it is not specific nor sensitive
TRUE, because it is more sensitive than slide coagulase test

✓ 22. A 31-year-old Asian woman is admitted to the hospital with a 7-week history of increasing malaise, myalgia, nonproductive
cough, and shortness of breath. She has daily fevers of 38–39°C and a recent 5-kg weight loss. She had a negative chest
Points: radiograph when she entered the United States 7 years ago. The patient’s grandmother died of tuberculosis when the patient was
1/1 an infant. A current chest radiograph is normal; results of other tests show a decreased hematocrit and liver function test
abnormalities. Liver and bone marrow biopsies show granulomas with giant cells and acid-fast bacilli. She is probably infected with:
Mycobacterium gordonae
Mycobacterium tuberculosis ✓
Mycobacterium ulcerans
Mycobacterium fortuitum

✓ 23. Identify the organism.

Points: It has group 1 cephalosporinase.


1/1 Culture : MAC – colonies become clear and colorless (NLF) after 24 hrs
Urease : +
IMViC : -+-+
TSI : A/A or K/A, gas (+), H2S (+)

C. freundii ✓
C. diversus
C. koseri
M. morganii
✘ 24. All of the following are components of LIA, except.

Points: Ammonium ✓
0/1 Peptones
Lysine
0.1% Glucose ✘

✘ 25. Reagent of choice for tube coagulase test.

Points: Citrate-collected plasma ✘


0/1 Serum
SPS-collected plasma
EDTA-collected plasma ✓

✓ 26. It is important to differentiate streptococci and enterococci because:

Points: they have different antibiotics that can affect them ✓


1/1 they have different mechanisms of infection
they have different levels of virulence
enterococci is an aggressive nosocomial pathogen, whereas streptococci is not

✓ 27. Which of the following cell components produced by Neisseria gonorrhoeae is responsible for attachment to host cells?

Points: Pili (fimbriae) ✓


1/1 Lipooligosaccharide
IgA1 protease
Outer membrane porin protein

✓ 28. Identify the statement if its TRUE OR FALSE.

Points: I. Enterobacteriaceae family are facultatively anaerobic, non-spore-forming, gram-negative bacilli.


1/1 II. All members ferment glucose and reduce nitrate to nitrite.

Both statements are true ✓


First statement is false ; Second statement is true
Both statements are false
First statement is true ; Second statement is false

✓ 29. What is the end product of Christensen's Method?

Points: Sodium thiosulfate


1/1 Ammonium carbonate ✓
Ferrous sulfate
Hydrogen sulfide

✘ 30. TRUE OR FALSE. Slide catalase test is preferable than tube catalase test.

Points: FALSE, because it is less sensitive than tube catalase test.


0/1 TRUE, because slide catalase test consumes less reagent. ✓
TRUE, because slide catalase test can be interpreted right away.
FALSE, because it is less specific than tube catalase test. ✘
✓ 31. Organisms that are lactose fermenters possesses what enzyme?

Points: B-galactoside permease and B-galactosidase ✓


1/1 B-galactoside permease
None of the choices
B-galactosidase

✓ 32. The MTOD performed the algorithm for gram-positive cocci and one of the test results is a jelly-like substance from defibrinated
plasma. What test is being interpreted?
Points:
1/1 slide coagulase test
catalase test
PYR test
tube coagulase test ✓

✓ 33. This test determines the organism’s ability to produce keratoconjunctivitis in guinea pigs. This is also a virulence test
for Shigella and EIEC.
Points:
1/1 String Test
Neufeld-Quellung Test
Sereny Test ✓
4-methylumbelliferyl-B-D-glucuronide

✓ 34. Which M. avium complex (MAC) organism is the most frequently isolated mycobacterium from AIDS patients?

Points: M. intracellulare
1/1 M. bovis
M. scrofulaceum
M. avium ✓

✘ 35. This organism has a sensitive result when tested with Vibriostatic test O/129.

Points: Escherichia coli ✘


0/1 Shigella sonnei
Plesiomonas shigelloides ✓
Edwardsiella tarda

✘ 36. Tests that can identify Streptococcus pyogenes.

Points: I. bacitracin test - 6 mm


0/1 II. optochin test - 6 mm
III. PYR hydrolysis - red color
I, III ✘
III
NONE OF THESE ✓
II, III

✘ 37. It is the hallmark of enteric fever infection during the second week of fever.

Points: Koplik spots


0/1 Rose spots ✓
Butterfly rash ✘
Forchheimer spots
✓ 38. Which organism is the most frequent cause of bacterial meningitis in neonates?

Points: Klebsiella pneumoniae


1/1 Neisseria meningitidis
Streptococcus pneumoniae
Group B Streptococcus ✓

✘ 39. TRUE OR FALSE. Enterococcus can grow on MAC.

Points: False, it is not capable of growth in MAC ✘


0/1 True, if it's subjected to prolonged incubation ✓
NONE OF THESE
True, because enterococcus is inherently resistant to all antibiotics and inhibitors

✓ 40. The function of N-acetyl-L-cysteine in the reagent for acid-fast digestion-decontamination procedure is to:

Points: Inhibit the growth of fungi


1/1 neutralize sodium hydroxide
inhibit growth of normal respiratory flora
Liquefy the mucus ✓

✓ 41. 1. A child was rushed into the hospital due to malaise, anorexia, myalgia and severe frontal headache, the doctor suspected
Points: that the child have enteric fever. The laboratory tests were requested and these are the following results.
1/1 Laboratory Results:
Culture : MAC – colonies are clear and colorless (NLF)
SSA – colonies are colorless with black centers
Urease : -
IMViC : -+--
TSI : K/A, gas (-), H2S (+)

Based on the laboratory results, what is the organism isolated?


Salmonella ✓
Citrobacter
Shigella
Morganella

✓ 42. Identify the statement if it is TRUE or FALSE.

Points: I. In a K/A reaction in TSI, this means that the glucose is depleted.
1/1 II. In an A/A reaction in TSI, this means that the enterobacteria attacks the lactose and sucrose first and then the glucose.
Both statements are true
First statement is true ; Second statement is false ✓
First statement is false ; Second statement is true
Both statements are false
✓ 43. A 19-year-old man presented to the clinic with a urethral discharge for the past 24 hours. Neisseria gonorrhoeae was cultured
from the specimen and found to be β-lactamase positive and resistant to high levels (≥32 μg/mL) of tetracycline. Which of the
Points: following statements about these antimicrobial resistance factors is correct?
1/1
Whereas β-lactamase production is mediated by a gene on the bacterial chromosome, high-level tetracycline
resistance is mediated by a gene on a plasmid.

β-lactamase production and high-level resistance to tetracycline are both mediated by genes on the bacterial chromosome.
β-lactamase production and high-level resistance to tetra-cycline are both mediated by genes on plasmids.
Whereas β-lactamase production is mediated by a gene on a plasmid, high-level tetracycline resistance is mediated by a
gene on the bacterial chromosome.

✓ 44. Tubercle bacilli are specifically stained by:

Points: Methylene bukas


1/1 Crystal violet
1% acid fuchsin
Carbol fuchsin ✓

✓ 45. Streptococcus mutans.

Points: most commonly isolated Viridans streptococci


1/1 most common cause cause dental carries and bacteremia among Viridans streptococci
ALL OF THESE ✓
NONE OF THESE

✘ 46. Which of the statements is true about the Kligler Iron Agar?

Points: I. It has the same components as those of TSI except for glucose.
0/1 II. It contains ten times more sucrose than glucose as compared with TSI.
I only
II only
None of the choices ✓
I and II ✘

✘ 47. It is used to confirm a negative reaction in Nitrate Reduction Test.

Points: Formation or acetoin ✘


0/1 Gelatin
Zinc dust ✓
Azo dye

✓ 48. Causative agent of Buruli ulcer.

Points: M. tuberculosis
1/1 M. marinum
M. ulcerans ✓
M. chelonei
✘ 49. An 18-year-old woman who reports unprotected sex with a new partner 2 weeks previously develops fever and left lower
quadrant abdominal pain with onset in association with her menstrual period. On pelvic examination in the emergency department,
Points: she has bilateral tenderness when the uterus is palpated. A mass 2–3 cm in diameter is felt on the left, suggestive of tubo-ovarian
0/1 abscess. Subsequently, Neisseria gonorrhoeae is cultured from her endocervix. The diagnosis is gonococcal pelvic inflammatory
disease. A common sequela of this infection is
Urethral stricture
Uterine fibroid tumors ✘
Infertility ✓
Cancer of the cervix

✓ 50. It is very important that a patient diagnosed with tuberculosis can also be evaluated for

Points: HIV/AIDS ✓
1/1 Liver abscess
Lymphoma
Typhoid fever

✘ 51. What organism produces susceptibility or any zone of inhibition to Taxo A test?

Points: group C streptococcus


0/1 ALL OF THESE ✓
group A streptococcus ✘
group G streptococcus

✓ 52. It is a heme enzyme that catalyzes the decomposition of H2O2 to water and oxygen, and its positive reaction.

Points: PYR - red compound


1/1 coagulase - clot formation
NONE OF THESE
catalase - effervescence ✓

✘ 53. What is the pH indicator of MacConkey-Sorbitol agar?

Points: Phenol Red ✘


0/1 Citrate Blue
Bromthymol Blue
Neutral Red ✓

✘ 54. A physician examined a patient's wound and noticed that it is a black, leathery tissue with foul smelling discharge. Direct C/S
reveals gram-positive cocci is present on the wound. The most likely causative agent is resistant to ____________.
Points:
0/1 NONE OF THESE
ALL OF THESE
taxo P ✓
taxo A ✘

✘ 55. You observe a 40-year-old man begging on a street in a town in India. He has clawing of the fourth and fifth digits with loss of
distal parts of the digits of both hands, strongly suggesting leprosy. The causative agent of this disease
Points:
0/1 Grows in parts of the body that are cooler than 37°C ✓
Is susceptible to isoniazid and rifampin
Can be cultured in the laboratory using Middlebrook 7h11 Medium ✘
Is seen in high numbers in biopsies of tuberculoid leprosy lesions
✓ 56. A 47-year-old woman presents with a 3-month history of progressive cough, weight loss, and fever. Chest radiography shows
bilateral cavitary disease suggestive of tuberculosis. Sputum culture grows an acid-fast bacillus that is a photochromogen (makes
Points: an orange pigment when exposed to light). The organism most likely is
1/1
Mycobacterium tuberculosis
Mycobacterium kansasii ✓
Mycobacterium avium complex
Mycobacterium gordonae

✘ 57. TRUE OR FALSE. Staphylococcus epidermidis grows on MSA agar.

Points: True, but the colonies are pink ✓


0/1 False, no growth of S. epidermidis in MSA since it is inhibited ✘
True, but the colonies are colorless
NONE OF THESE

✘ 58. A useful test to differentiate Moraxella catarrhalis from saprophytic neisseriae in respiratory samples is

Points: Growth on 5% sheep blood agar ✘


0/1 Gram stain
PYR
Butyrate esterase ✓

✘ 59. Staphylococcus enterotoxins cause:

Points: Scalded Skin Syndrome


0/1 food poisoning ✓
Toxic Shock Syndrome ✘
septic arthritis

✓ 60. Identify the statement if its TRUE or FALSE.

Points: I. Citrate Utilization Test is used to determine the ability of the organism to utilize sodium citrate as its only carbon source
1/1 and inorganic ammonium salts as its only nitrogen source.
II. If citrate is used as a carbon source, the medium will turn alkaline.
First statement is true ; Second statement is false
Both statements are false
Both statements are true ✓
First statement is false ; Second statement is true

✘ 61. A spreading-factor enzyme.

Points: duran reynal factor ✓


0/1 staphylokinase ✘
lipase
DNase
✓ 62. Identify the organism.

Points: Laboratory results :


1/1 Microscopy : short, plump rod with a bipolar body or safety pin appearance
Culture :
MAC – colonies are clear and colorless
BAP – colonies are pinpoint at 24 hours
Broth – colonies have a stalactite-shaped pattern
IMViC : -+--
TSI : K/A, gas (-), H2S (-)

Y. pestis ✓
Y. enterocolitica
Plesiomonas
Y. pseudotuberculosis

✘ 63. A vaginal smear is submitted for Gram stain for Neisseria gonorrhoeae. The technologist finds the ONLY following results on
the Gram stain:
Points:
0/1 many white blood cells
few epithelial cells
many gram-positive bacilli
few gram-negative diplococci
few gram-positive cocci in chains
The technologist should:
do not read or report a Gram stain on a vaginal specimen ✓
report out smear positive for gonorrhea ✘
report out smear negative for gonorrhea
request a new specimen due to number of white blood cells

✓ 64. Staphylococcus aureus can be confirmed using:

Points: DNase test


1/1 MSA subculture result
ALL OF THESE ✓
tube coagulase test
✘ 65. β-Hemolytic streptococci, more than 50,000 col/mL, were isolated from a urinary tract catheter urine specimen. Given the
following reactions, what is the most likely identification?
Points:
0/1 CAMP test = neg
Hippurate hydrolysis = ±
Bile solubility = neg
6.5% salt = pos
PYR = pos
Bile esculin = pos
SXT = Resistant
Bacitracin = Resistant
Optochin = Resistant
Enterococcus faecalis ✓
nongroup A, nongroup B, nongroup D streptococci
group A streptococci
group B streptococci ✘

✘ 66. A 33 year old man comes to the ER complaining about his cola-colored urine so the physician orders a urinalysis, and some
other tests. The MTOD in Clinical Microscopy observes that the urine sample has many RBC casts, dysmorphic RBCs, and
Points: increased protein so he releases the results. When the physician sees all the results, she concludes that the patient is suffering
0/1 from Post-streptococcal acute glomerulonephritis. What is the most deciding factor of the physician for her final diagnosis?
her gut instincts
px medical history
other tests (e.g. ASO titer) ✓
urinalysis result ✘

✓ 67. According to Bergey's Academic classification, Viridans streptococcus can grow at what temperature?

Points: 37 degrees fahrenheit


1/1 37 degrees celcius
45 degrees celcius ✓
45 degrees fahrenheit

✘ 68. A first morning sputum is received for culture of mycobacteria. It is digested and concentrated by the N-acetyl-L-cysteine alkali
method. Two Lowenstein-Jensen slants are incubated in the dark at 35°C with 5%-10% CO, The smears reveal acid-fast bacilli,
Points: and after 7 days no growth appears onthe slants. The best explanation is:
0/1
exposure to CO₂ prevents growth
improper specimen submitted
incorrect concentration procedure ✘
cultures held for insufficient length of time ✓

✘ 69. A test used to differentiate P. rettgeri and P. stuartii

Points: TSI
0/1 Urease ✓
PAD ✘
Indole
✓ 70. The MTOD processed a urine sample and during direct gram-staining, he saw that the bacteria is gram+ spheres. There is
growth on BAP but no growth on MAC. How will the MTOD proceed?
Points:
1/1 look at the hemolytic pattern
do indirect gram staining
perform catalase test ✓
perform TSI innoculation

✘ 71. I. Staphylococci associated with Prosthetic Valve Endocarditis.

Points: II. Staphylococci associated with UTI in sexually active females.


0/1 I. 16 mm clearing with Bacitracin disk
II. 12 mm clearing with Bacitracin disk ✘

I. 12 mm clearing with Bacitracin disk


II. 16 mm clearing with Bacitracin disk

I. 16 mm clearing with Novobiocin disk


II. 12 mm clearing with Novobiocin disk ✓

I. 12 mm clearing with Novobiocin disk


II. 16 mm clearing with Novobiocin disk

✓ 72. What is the percentage of chocolate in Chocolate agar?

Points: 0% ✓
1/1 5%
0.5%
20%

✓ 73. An organism considered as a Class A bioterrorism agent.

Points: Y. pestis ✓
1/1 E. coli
S. typhi
S. dysenteriae

✓ 74. A positive Mantoux test indicates an area of induration of:

Points: 4-9 mm in diameter


1/1 10 mm or above in diameter ✓
None of these
1-4 mm in diameter

✓ 75. Which organism, associated with tuberculosis in cattle, causes tuberculosis in humans, especially in regions where dairy
farming is prevalent?
Points:
1/1 Mycobacterium avium–intracellulare complex
Mycobacterium kansasii
Mycobacterium marinum
Mycobacterium bovis ✓
✓ 76. Identify the organism.

Points: Culture: MAC – colonies exhibits a pink color (LF)


1/1 Neufeld-Quellung test : Positive
TSI : A/A, gas (+), H2S (-)
IMVic Reaction : --++
Pantoea agglomerans
Serratia marcescens
Escherichia coli
Klebsiella pneumoniae subsp. pneumoniae ✓

✓ 77. The Superoxol test is used as a rapid presumptive test for:

Points: Neisseria meningitidis


1/1 Moraxella (Branhamella) catarrhalis
Neisseria lactamica
Neisseria gonorrhoeae ✓

✘ 78. In XLD agar, differentiate the growth produced by Salmonella and Shigella.

Points: Salmonella - colonies exhibit a pink to red color : Shigella - colonies exhibit a pink to red color with black centers ✓
0/1 Salmonella - colonies exhibit a orange to red color : Shigella - colonies exhibit a orange to red color with black centers
Salmonella - colonies exhibit a red to pink color : Shigella - colonies exhibit a red to pink color with black
centers

Salmonella - colonies exhibit a white to blue color : Shigella - colonies exhibit a white to blue color with black centers

✓ 79. A 60-year-old-man has a 5-month history of progressive weakness and a weight loss of 13 kg along with intermittent fever,
chills, and a chronic cough productive of yellow sputum, occasionally streaked with blood. A sputum specimen is obtained, and
Points: numerous acid-fast bacteria are seen on the smear. Culture of the sputum is positive for Mycobacterium tuberculosis. Which
1/1 treatment regimen is most appropriate for initial therapy?
Isoniazid and rifampin
Sulfamethoxazole–trimethoprim and streptomycin
Isoniazid, cycloserine, and ciprofloxacin
Isoniazid, rifampin, pyrazinamide, and ethambutol ✓

✘ 80. The best specimen for recovery of the mycobacteria a sputum sample is:

Points: 12-hour pooled specimen


0/1 10-hour evening specimen
24-hour pooled specimen ✘
First morning specimen ✓

✓ 81. Which of the following is the most reliable test to differentiate Neisseria lactamica from Neisseria meningitidis?

Points: lactose degradation ✓


1/1 acid from maltose
growth on modified Thayer-Martin agar
nitrite reduction to nitrogen gas
✘ 82. Jet black colonies in Tellurite Glycine Agar.

Points: Staphylococcus epidermidis


0/1 Staphylococcus aureus ✓
Escherichia coli
Salmonella typhi ✘

✘ 83. The β-galactosidase test aids in the identification of which Neisseria species?

Points: N. flavescens
0/1 N. lactamica ✓
N. gonorrhoeae
N. meningitidis ✘

✘ 84. group A streptococci exhibits what type of hemolysis in MAC?

Points: alpha
0/1 no hemolysis
not applicable ✓
beta ✘

✘ 85. I. In the Philippines, Streptococcus agalactiae testing is included in Newborn screening.

Points: II. In the Philippines, Streptococcus agalactiae colonization of the vagina is routinely done to expecting mothers near birth.
0/1 Which of the following statement/s is/are not incorrect?
II
I
ALL OF THESE ✘
NONE OF THESE ✓

✘ 86. It is the only oxidase-positive member of Enterobacteriaceae and it has been associated with HIV-positive individuals with
inflammatory bowel disease.
Points:
0/1 Y. pseudotuberculosis
Y. enterocolitica ✘
Y. pestis
Plesiomonas ✓

✘ 87. TRUE OR FALSE. Streptococcus pyogenes exhibits positive reaction in CAMP test because it enhances the beta-hemolysis of
Staphyloccocus aureus.
Points:
0/1 FALSE, S. pyogenes does not exhibit positive reaction with S. aureus
FALSE, S. pyogenes is not CAMP test positive ✘
TRUE, because S. pyogenes has diffusible, extracellular protein ✓
TRUE, because S. pyogenes has diffusible, intracellular protein

✘ 88. Causative agent of Erythema nodosum.

Points: Shigella spp. ✘


0/1 Serratia marcescens
Yersinia enterocolitica ✓
Salmonella spp.
✓ 89. A 25-year-old woman presents with septic arthritis of the knee. The fluid aspirate grows a gram-negative diplococcus on
chocolate agar after 48 hours of incubation. The isolate is oxidase positive and oxidizes glucose but not maltose, lactose, or
Points: sucrose. You suspect infection with:
1/1
Neisseria meningitidis
Neisseria gonorrhoeae ✓
Moraxella catarrhalis
Neisseria lactamica

✘ 90. Tests that can identify Streptococcus pneumoniae.

Points: I. Optochin test - 20 mm zone of inhibition


0/1 II. Inulin test - gas formation
III. bile solubility - clear area
I, III
I, II, III ✓
I✘
I, II

✘ 91. The preferred carbon source for mycobacteria is:

Points: Glycerol
0/1 Glucose ✘
Fatty acids ✓
Casein Hydrokysate

✓ 92. Streptococcus viridans usually cause:

Points: endocarditis
1/1 ALL OF THESE ✓
gingivitis
dental carries

✓ 93. What are the deciding factors that can affect the integrity of BAP during agar preparation?

Points: I. ratio of the lyophisized agar and distilled water


1/1 II. sterilization method
III. temperature during the addition of blood
IV. blood type of the blood to be added
ALL OF THESE ✓
I
NONE OF THESE
III

✓ 94. A Gram stain of a urethral discharge from a man showing extracellular and intracellular gram-negative diplococci within
segmented neutrophils is a presumptive identification for:
Points:
1/1 Neisseria lactamica
Moraxella (Branhamella) catarrhalis
Neisseria meningitidis
Neisseria gonorrhoeae ✓
✓ 95. In TSI reaction, after completing 18-24 hours of incubation, the organism has utilized the peptones in the slant, which results in
the formation of alkaline products and the change of the slant color to ________.
Points:
1/1 Blue
Red ✓
Black
Yellow

✓ 96. This organism exhibits a green metallic sheen colonies on EMB.

Points: Enterbacter aerogenes


1/1 Escherichia coli ✓
Klebsiella pneumoniae
Cronobacter sakazakii

✘ 97. Organism that exhibits a positive result in gelatin liquefaction test.

Points: Edwardsiella
0/1 Serratia ✓
Yersinia
E.coli

✘ 98. It is an extracellular enzyme that causes clot formation when bacterial cells are incubated with plasma.

Points: clumping factor ✘


0/1 NONE OF THESE
staphylocoagulase ✓
split coagulase

✓ 99. What is the desired specimen for Salmonella identification during the second to third week of infection?

Points: Urine
1/1 Stool ✓
Blood
CSF

✓ 100. Cause of false-positive with CAMP test.

Points: incubation if candle jar ✓


1/1 wrong temperature during incubation
BAP is not prepared correctly
prolonged incubation

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