Scribd Logo
Upload

Welcome to Scribd!

  • 37 views

    2midterm 9 - Molecular Biology TRANS

    Uploaded by

    Robee Camille Desabelle-Sumatra
    This document discusses molecular biology techniques, with a focus on polymerase chain reaction (PCR). It defines molecular biology and describes several techniques used, including PCR. It explains that PCR is used to amplify DNA or RNA sequences and relies on thermal cycling to promote enzymatic replication. It notes some applications of PCR including genetic disorders, paternity testing, and infectious disease diagnosis. It states that PCR is 100% accurate in determining the infectious agent and discusses its use for pathogens like hepatitis, dengue, rabies, HIV, malaria, tuberculosis, and respiratory viruses.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    2midterm 9 - Molecular Biology TRANS

    Uploaded by

    Robee Camille Desabelle-Sumatra
    37 views3 pages
    This document discusses molecular biology techniques, with a focus on polymerase chain reaction (PCR). It defines molecular biology and describes several techniques used, including PCR. It explains that PCR is used to amplify DNA or RNA sequences and relies on thermal cycling to promote enzymatic replication. It notes some applications of PCR including genetic disorders, paternity testing, and infectious disease diagnosis. It states that PCR is 100% accurate in determining the infectious agent and discusses its use for pathogens like hepatitis, dengue, rabies, HIV, malaria, tuberculosis, and respiratory viruses.

    Original Title

    2Midterm 9 - Molecular Biology TRANS

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document discusses molecular biology techniques, with a focus on polymerase chain reaction (PCR). It defines molecular biology and describes several techniques used, including PCR. It explains that PCR is used to amplify DNA or RNA sequences and relies on thermal cycling to promote enzymatic replication. It notes some applications of PCR including genetic disorders, paternity testing, and infectious disease diagnosis. It states that PCR is 100% accurate in determining the infectious agent and discusses its use for pathogens like hepatitis, dengue, rabies, HIV, malaria, tuberculosis, and respiratory viruses.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    37 views3 pages

    2midterm 9 - Molecular Biology TRANS

    Uploaded by

    Robee Camille Desabelle-Sumatra
    This document discusses molecular biology techniques, with a focus on polymerase chain reaction (PCR). It defines molecular biology and describes several techniques used, including PCR. It explains that PCR is used to amplify DNA or RNA sequences and relies on thermal cycling to promote enzymatic replication. It notes some applications of PCR including genetic disorders, paternity testing, and infectious disease diagnosis. It states that PCR is 100% accurate in determining the infectious agent and discusses its use for pathogens like hepatitis, dengue, rabies, HIV, malaria, tuberculosis, and respiratory viruses.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    1|P a g e

    MOLECULAR BIOLOGY  It can be processed even in small quantity of


    Dr. Gilbert Vergara samples
     It is the method of choice for the rapid identification
    Definition of viral and non-cultivable infectious agents
     Deals with the study of structure and function of
    macromolecules (CHON and nucleic acid) Viral Nucleic Acid

    Techniques of Molecular Biology


     Expression cloning
     Polymerase chain reaction (PCR)
     Gel electrophoresis
     Macromolecule blotting and probing techniques
     Southern blotting
     Northern blotting
     Western blotting
     Eastern blotting
     Arrays Routine Specimens
     Allele specific oligonucleotide  CSF
     Antiquated technologies  Respiratory secretions
     Discharge
    Polymerase Chain Reaction (PCR)
     Pure Isolate (bacteria)
     Is a technique in molecular biology to amplify a  Urine
    single copy or a few copies of a segmented DNA
     Swabs
    across several orders of magnitude, generating
    thousands to millions of copies of a particular DNA
    Acceptable Clinical samples for TB PCR:
    sequence
     Sputum
     Basic information
     Gastric washing
     Role in molecular biology in diagnostics
     Stool
     Accuracy in diagnosis
     Urine
     PCR is a technique to amplify a piece of DNA or
     Paraffin block
    RNA very rapidly outside of a cell
     CSF
     The method relies entirely on thermal cycling
    or repeated heating and cooling to promote  BAL
    enzymatic replication  Tissue (no formalin)
     Uses enzyme of bacterium Thermus aquaticus  Wound discharge
    (Taq DNA polymerase)
     Some applications of PCR Unacceptable Clinical samples for TB PCR:
     Genetic disorders  Blood
     Paternity testing  Specimen in anaerobic vial
     Forensic medicine  Viral transport medium
     Infectious disease  Swab
     Tissue formalin fluid
    To pinpoint the real culprit:
     PCR is 100% accurate in determining the etiologic Important Pathogens:
    agent of an infectious disease  Hepatitis viruses
     Dengue

    asis, k
    2|P a g e

     Rabies  Annealing: alteration of the current structure of the


     HIV nucleic acid
     Malaria  Elongation: stretches the target nucleic acid t
     Tuberculosis (shorter TAT) basically insert the Taq gene
     Respiratory viruses
     Leprosy bacteria

    PCR Drawback:
     Processing of samples
     Aseptic technique
     Kits (expensive)
     Sample storage (very crucial in TB PCR)
     Temperature (kits)
     Special training for Medtech

    PCR vs Culture: which is more accurate for TB?


     Culture

    Basic Procedures
     100 ul reaction proceudre

    Principle and Procedure


     Denaturation: a process in which CHONs and NA
    lose the tertiary structure and secondary structures

    asis, k
    3|P a g e

    Reading the Results (PCR stages) Interpretaion of Results


     Copies (quantitative)
     Positive/Negative
     Detected/Not Detected

    PCR and Hepa C Virus


     Viral load monitoring thru copies (HCV)
     Can monitor drug effectivity (HCV)
     Can detect early sero-conversion thru decreased
    viral NA or viral load

    Which is more accurate in PCR


     DNA
     RNA
     Mitochondria

    Semi PCR: GeneXpert

    asis, k

    You might also like

    pFad - Phonifier reborn

    Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

    Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


    Alternative Proxies:

    Alternative Proxy

    pFad Proxy

    pFad v3 Proxy

    pFad v4 Proxy