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    Lab # 2

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    Martyn Pereira
    Martyn Pereira conducted an experiment on July 2nd, 2022 to test various food samples for the presence of starch using iodine solution. Samples of carrot, milk powder, baked potato, whole wheat flour, boiled rice, onion, beans, and cassava were crushed and placed on a spotting tile, to which iodine solution was added. Observations were recorded, finding that the baked potato, beans, cassava, whole wheat flour, and rice turned blue-black, indicating the presence of starch, while the carrot, milk powder, and onion did not. Starch is made of glucose monomers that form straight chains or branches, which iodine binds to in a spiral shape, appearing blue

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    Lab # 2

    Uploaded by

    Martyn Pereira
    1K views4 pages
    Martyn Pereira conducted an experiment on July 2nd, 2022 to test various food samples for the presence of starch using iodine solution. Samples of carrot, milk powder, baked potato, whole wheat flour, boiled rice, onion, beans, and cassava were crushed and placed on a spotting tile, to which iodine solution was added. Observations were recorded, finding that the baked potato, beans, cassava, whole wheat flour, and rice turned blue-black, indicating the presence of starch, while the carrot, milk powder, and onion did not. Starch is made of glucose monomers that form straight chains or branches, which iodine binds to in a spiral shape, appearing blue

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    Martyn Pereira conducted an experiment on July 2nd, 2022 to test various food samples for the presence of starch using iodine solution. Samples of carrot, milk powder, baked potato, whole wheat flour, boiled rice, onion, beans, and cassava were crushed and placed on a spotting tile, to which iodine solution was added. Observations were recorded, finding that the baked potato, beans, cassava, whole wheat flour, and rice turned blue-black, indicating the presence of starch, while the carrot, milk powder, and onion did not. Starch is made of glucose monomers that form straight chains or branches, which iodine binds to in a spiral shape, appearing blue

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    1K views4 pages

    Lab # 2

    Uploaded by

    Martyn Pereira
    Martyn Pereira conducted an experiment on July 2nd, 2022 to test various food samples for the presence of starch using iodine solution. Samples of carrot, milk powder, baked potato, whole wheat flour, boiled rice, onion, beans, and cassava were crushed and placed on a spotting tile, to which iodine solution was added. Observations were recorded, finding that the baked potato, beans, cassava, whole wheat flour, and rice turned blue-black, indicating the presence of starch, while the carrot, milk powder, and onion did not. Starch is made of glucose monomers that form straight chains or branches, which iodine binds to in a spiral shape, appearing blue

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 4

    Name: Martyn Pereira

    Date: 07/02/2022

    Lab #: 2

    Title: Starch test

    Aim: To test food samples for the presence of starch

    Background information: a food test is a procedure that is implemented with the purpose of

    determining the components within foods. In the test for starch, the reagent used is iodine

    solution because when it comes into contact with a sample with traces of starch, it produces a

    blue black colour as a positive indication.

    Apparatus:

    1. 1 spotting tile

    2. 1 teat pipette

    3. 1 mortar and pestle

    4. 1 spatula
    Materials:

    1. Carrot

    2. Milk Powder

    3. Baked Potato

    4. Whole Wheat Flour

    5. Boiled Rice

    6. Onion

    7. Beans

    8. Cassava

    Reagent:

    1. Iodine solution

    Diagram:
    Method:

    1. All apparatus were cleaned before use.

    2. A small amount of each food sample was crushed.

    3. Using a spatula, a small amount of the crushed food sample was placed onto an empty

    slot on the spotting tile.

    4. Two to three drops of iodine solution were added to each food sample on the spotting

    tile.

    5. All observations were recorded in tabular form.

    Discussion: Starch is a polysaccharide, which is made up of many monosaccharides. It is

    used in the storage of energy in plants. There are two types of starch, namely: amylose and

    amylopectin. Amylose is made up of many alpha glucose monomer linkages, which produces

    a straight chain structure consisting of 1,4 glycosidic bonds between the first and fourth

    carbon of each glucose molecule. Amylopectin also consists of alpha glucose monomer

    linkages consisting of a straight chain connected by 1,4 glycosidic bonds between the first

    and fourth carbon, and branched chains consisting of 1,6 glycosidic bonds between first and

    sixth carbon, situated above the straight chain. In this experiment, when the crushed food

    material had come into contact with iodine solution, it produced a blue black colour to

    indicate a positive presence of starch. This technique usually targets amylose, whose bond

    angle is in such a way that it is shaped like a spiral. Since iodine is not soluble in water, it is

    made to dissolve in the presence of potassium iodide producing a linear trioxide soluble

    compound, which slips into the coil producing an intense blue black colour.
    Sources of error:

     Materials were not finely ground

    Precautions:

     Clean all apparatus to reduce risk of contamination.

     Provide indication as to which well is being used.

    Conclusion: It can be concluded that the baked potato, beans, cassava, whole wheat flour,

    and rice contained starch, since they produced a blue-black colour with the iodine solution

    while the carrot, milk powder, and onion did not, and as such did not contain starch.

    References:

     Vedantu. (2022, February 2). Difference Between Starch and Cellulose.

    https://www.vedantu.com/chemistry/difference-between-starch-and-cellulose

     Libretexts. (2020, August 11). Starch and Iodine. Chemistry LibreTexts.

    https://chem.libretexts.org/Bookshelves/Biological_Chemistry/Supplemental_Module

    s_(Biological_Chemistry)/Carbohydrates/Case_Studies/Starch_and_Iodine

     A. (2016, October 2). Iodine test for Starch- Its Principle, Reagents, Procedure etc.

    All Medical Tests. https://allmedtests.com/iodine-test-starch/

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