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    Praxib 2

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    Retro Girl
    This document describes the key steps in agarose gel electrophoresis of DNA, including: 1) Electrophoresis separates DNA fragments based on size and charge by applying an electric current through an agarose gel. 2) Loading gel samples adds density to track sample migration and identify/estimate protein and nucleic acid movement. 3) Agarose gel density is adjusted based on desired fragment size separation - lower density separates large fragments, higher density separates smaller fragments.

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    Praxib 2

    Uploaded by

    Retro Girl
    34 views5 pages
    This document describes the key steps in agarose gel electrophoresis of DNA, including: 1) Electrophoresis separates DNA fragments based on size and charge by applying an electric current through an agarose gel. 2) Loading gel samples adds density to track sample migration and identify/estimate protein and nucleic acid movement. 3) Agarose gel density is adjusted based on desired fragment size separation - lower density separates large fragments, higher density separates smaller fragments.

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    praxib 2

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    This document describes the key steps in agarose gel electrophoresis of DNA, including: 1) Electrophoresis separates DNA fragments based on size and charge by applying an electric current through an agarose gel. 2) Loading gel samples adds density to track sample migration and identify/estimate protein and nucleic acid movement. 3) Agarose gel density is adjusted based on desired fragment size separation - lower density separates large fragments, higher density separates smaller fragments.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    34 views5 pages

    Praxib 2

    Uploaded by

    Retro Girl
    This document describes the key steps in agarose gel electrophoresis of DNA, including: 1) Electrophoresis separates DNA fragments based on size and charge by applying an electric current through an agarose gel. 2) Loading gel samples adds density to track sample migration and identify/estimate protein and nucleic acid movement. 3) Agarose gel density is adjusted based on desired fragment size separation - lower density separates large fragments, higher density separates smaller fragments.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 5

    2.3.2.

    7 PraxiLab: Agarose Gel Electrophoresis of DNA

    Applications of method:

    Screenshots of at least 5 steps in the protocol, and why each step is


    important towards the achievement of the desired outcome(s) of this
    method/technique.
    Image Importance of
    step
    ELECTROPHORES
    IS

    This step
    involves a
    technique that
    uses an electric
    current to
    separate DNA,
    RNA, or proteins
    based on
    physical
    properties such
    as size and
    charge.
    Negatively
    charged
    DNA/RNA
    migrates through
    the pores of the
    agarose gel to
    the positively
    charged end of
    the gel when an
    electric current is
    applied, and
    smaller
    fragments
    migrate faster.
    The resulting
    bands can be
    visualized with
    ultraviolet (UV)
    light.
    LOADING GEL
    SAMPLES

    Loading gel
    samples into the
    gel wells adds
    density to the
    sample. Also, it
    help to track the
    running of
    samples in the
    gel
    electrophoresis.
    They help to
    identify and
    estimate the
    migration of
    proteins and
    nucleic acids.
    WEIGHING THE
    AGAROSE GEL

    Density plays a
    vital role in this
    step. A low
    density of
    agarose is used
    to separate large
    fragments,
    whereas a high
    density is used to
    separate smaller
    fragments.
    However, when
    two fragments
    are of similar
    size, adjusting
    the percentage
    of agarose will
    not help much to
    improve the
    separation
    PUTTING THE
    AGAROSE GEL
    INSIDE THE
    TRANSLLUMINAT
    OR

    UV
    transilluminators
    are used in
    molecular
    biology
    laboratories to
    display DNA or
    RNA separated
    by
    electrophoresis
    through agarose
    gels. When the
    stained gel is
    exposed to a UV
    light source, the
    DNA fluoresces
    and becomes
    visible. UV
    transilluminators
    work by emitting
    high levels of UV
    radiation from a
    viewing surface
    on which a wet
    agarose gel is
    placed. The gel is
    stained with a
    fluorescent dye
    that binds to
    nucleic acids,
    causing the dye
    to fluoresce
    when exposed to
    a UV light
    source.
    HEATING THE
    AGAROSE

    In this step, gels


    are prepared by
    heating agarose
    in appropriate
    buffers. Gels
    contain
    microscopic
    pores that act
    like molecular
    sieves. Certain
    molecules may
    also interact with
    agarose to
    varying degrees,
    affecting its
    mobility. The
    higher the
    agarose
    concentration,
    the higher the
    resolution.

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