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    Exp No 8 Controlled Pollination

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    Shadab Ahmad
    Controlled pollination is a plant breeding technique used to produce progeny with desired traits from known parent plants. It involves transferring pollen from the male parent to the receptive female organs of another plant while excluding all other pollen. The process includes emasculation to remove stamens, bagging the flower to isolate it, and tagging for identification. After controlled pollination, the plant is observed until seed setting to ensure the success of producing seeds with the desired characteristics from the selected parents.

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    Exp No 8 Controlled Pollination

    Uploaded by

    Shadab Ahmad
    7K views3 pages
    Controlled pollination is a plant breeding technique used to produce progeny with desired traits from known parent plants. It involves transferring pollen from the male parent to the receptive female organs of another plant while excluding all other pollen. The process includes emasculation to remove stamens, bagging the flower to isolate it, and tagging for identification. After controlled pollination, the plant is observed until seed setting to ensure the success of producing seeds with the desired characteristics from the selected parents.

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    Controlled pollination is a plant breeding technique used to produce progeny with desired traits from known parent plants. It involves transferring pollen from the male parent to the receptive female organs of another plant while excluding all other pollen. The process includes emasculation to remove stamens, bagging the flower to isolate it, and tagging for identification. After controlled pollination, the plant is observed until seed setting to ensure the success of producing seeds with the desired characteristics from the selected parents.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    7K views3 pages

    Exp No 8 Controlled Pollination

    Uploaded by

    Shadab Ahmad
    Controlled pollination is a plant breeding technique used to produce progeny with desired traits from known parent plants. It involves transferring pollen from the male parent to the receptive female organs of another plant while excluding all other pollen. The process includes emasculation to remove stamens, bagging the flower to isolate it, and tagging for identification. After controlled pollination, the plant is observed until seed setting to ensure the success of producing seeds with the desired characteristics from the selected parents.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 3

    AND TAGGING FOR CONTROLLED

    8.1 TO PERFORM EMASCULATION, BAGGING


    POLLINATION
    hat receives genes from each of
    to produce progeny
    Controlled pollination is a technique
    used in plant improvement
    pollen from
    one plant to the receptive female
    Controlled pollination consists of transferring
    two known parent plants.
    while excluding all other pollen.
    reproductive organs of another plant

    Aim
    emasculation, bagging and tagging for controlled pollination.
    To perform
    Materials Required
    flowers Magnifying lens
    Ornamental plants/ wild plants bearing large bisexual
    Brush
    Tweezers Small sharp scissors
    Alcohol Rubber bands Paper bags
    Paper clips Tags

    Theory/Principle that lead to better seed


    programmes involve
    under human control reproductive processes
    bringing
    Plant breeding female parents having desired
    is desirable using male and
    and better fruit formation. For this, controlled pollination the knowledge of flower
    under human control. For this
    traits. Emasculation is the process that can be easily brought
    essential. In emasculation technique,
    structure, mechanisnm of pollination,
    fertilisation and physiology of flowering is
    male parent is transferred
    female parent and pollen from the desired
    the stamens are removed before anthesis to obtain
    on to its stigma.

    Procedure
    Select a good variety of a plant.
    Now, select aflower in bud condition where antheses has not occurred.

    the bud carefully and remove the


    stamens (See fig.). Mark this as female parent plant.
    Open

    Scissor

    Stamens being removed

    Showing Emasculation of a flower

    BIoLOGY-12

    160 ACTICAL SKILLSI N


    Cover the emasculated tlower with a
    nollen (Bagging) (See fig.). Tie the
    transparent plastic bag with minute pores in it to sired
    bag in place with a paper pioc
    clip/string/thread.
    Plastic bag

    ..
    ..

    Female flower

    Bagging of an emasculated female flower

    Now, bring into physical contact anthers of a desired male plant containing mature pollen grains with the stigmatic
    surface of emasculated female flower (see fig.). Use tweezers/brush if necessary to dust the stigmatic surface
    with pollen.

    Stigma of female plant

    . **.
    . .

    Dusting of pollens of male plant

    pollination on an emasculated female flower


    cross
    Showing
    bag immediately, till seed setting is completed in the flower.
    the
    Again, cover the pollinated flower again with
    should be fixed below the flower.
    a tag (label)
    After controlled pollination,
    and
    seed parent, the letter A (to signify a cross), the name of the pollen parent,
    The tag contains the name of the
    effected.
    the date on which the cross was

    161
    PRACTICAL SKILS IN BIoLOGY-12
    Observations
    undisturbed and under observation
    till seed setting and harvestino Thiis
    the plant is kept
    After controlled pollination,
    leads to the success of
    the experiment.

    Result
    plant will produce seeds of the desired characteristics
    process of
    controlled pollination, the female parent
    By the

    APrecautions
    be selected.
    A good variety of parent plant should
    Care should be taken while doing emasculation.
    should be done till seed setting.
    A f t e r controlled pollination. proper care of experimental plant
    Suggested Experiment
    in the plant of your choice to get a hybrid plant.
    1. To perform the process of emasculation, bagging and tagging

    VIVA VoCE
    Q1. What is plant breeding?
    purposely crossed to produce a new hybrid
    Ans. It is the process in which two genetically dissimilar varieties are

    variety.
    Q2. Which is the oldest method of plant improvement?
    Ans. Selection
    Q3. What are the objectives of plant breeding?
    resistance to diseases, climatic adaptability.
    Ans. It aims to produce a new variety with the desired characteristics such as
    and better productivity.
    Q4. What is emasculation?
    anthers from bisexual flowers without affecting the female reproductive
    Ans. Emasculation is the process of removing
    part (pistil).
    Q5. Why is bagging done?
    emasculated flower.
    Ans. It is done to isolate the

    NCERT LAB MANUAL QUESTIONS F


    anthesis?
    Q1. Why is emasculation performed before
    Ans. During anthesis, pollen grains are released which lead to pollination ofthe flower. So, before anthesis emasculation
    must be done to get female flower ready for cross-pollination.

    Q2. What are the advantages of using a bag containing minute pores?
    Ans. A plastic bag with minute pores allows the gaseous exchange needed for the plant but protect it from undesired
    pollen.

    162 PRACHCAL SKLLs IN BiOLOGY-12

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