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    Introduction

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    Anita Torres
    This document provides information about the Molecular Genetics and Bioinformatics course taught by Bjorn Johansson. It includes the instructor's contact information and office hours, as well as links to course materials hosted on Dropbox. The course literature covers 16 chapters on topics related to gene cloning and DNA analysis. Tests will evaluate both theoretical and practical bioinformatics concepts taught in classes. Students are required to have a laptop computer to complete technical practical assignments, which will involve in silico techniques like sequence analysis and molecular cloning simulations.

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    Introduction

    Uploaded by

    Anita Torres
    27 views13 pages
    This document provides information about the Molecular Genetics and Bioinformatics course taught by Bjorn Johansson. It includes the instructor's contact information and office hours, as well as links to course materials hosted on Dropbox. The course literature covers 16 chapters on topics related to gene cloning and DNA analysis. Tests will evaluate both theoretical and practical bioinformatics concepts taught in classes. Students are required to have a laptop computer to complete technical practical assignments, which will involve in silico techniques like sequence analysis and molecular cloning simulations.

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    This document provides information about the Molecular Genetics and Bioinformatics course taught by Bjorn Johansson. It includes the instructor's contact information and office hours, as well as links to course materials hosted on Dropbox. The course literature covers 16 chapters on topics related to gene cloning and DNA analysis. Tests will evaluate both theoretical and practical bioinformatics concepts taught in classes. Students are required to have a laptop computer to complete technical practical assignments, which will involve in silico techniques like sequence analysis and molecular cloning simulations.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    27 views13 pages

    Introduction

    Uploaded by

    Anita Torres
    This document provides information about the Molecular Genetics and Bioinformatics course taught by Bjorn Johansson. It includes the instructor's contact information and office hours, as well as links to course materials hosted on Dropbox. The course literature covers 16 chapters on topics related to gene cloning and DNA analysis. Tests will evaluate both theoretical and practical bioinformatics concepts taught in classes. Students are required to have a laptop computer to complete technical practical assignments, which will involve in silico techniques like sequence analysis and molecular cloning simulations.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 13

    Genética Molecular

    & Bioinformática
    2704N9
    Docente

    Hor. atend.: Sexta-feira 16:00 – 17:00


    bjorn_johansson@bio.uminho.pt
    How do we define Molecular genetics and how does it relate to
    bioinformatics?
    Dropbox with *all* course files
    http://bit.ly/GenMolBio20

    https://www.dropbox.com/sh/rxs957obgo8jb17/AABiklpcEWIUBGDT8GKaPN2Qa?dl=0
    https://qr-creator.com
    Schedule BA
    Schedule BQ
    Course literature

    1 Why Gene Cloning and DNA Analysis are Important


    2 Vectors for Gene Cloning: Plasmids and Bacteriophages
    3 Purification of DNA from Living Cells
    4 Manipulation of Purified DNA
    5 Introduction of DNA into Living Cells
    6 Cloning Vectors for E. coli
    7 Cloning Vectors for Eukaryotes (7.1.1 – 7.1.5)
    8 How to Obtain a Clone of a Specific Gene
    9 The Polymerase Chain Reaction
    10 Sequencing Genes and Genomes
    11 Studying Gene Expression and Function
    12 Studying Genomes (12.1.1-12.2.2)
    13 Production of Protein from Cloned Genes
    14 Gene Cloning and DNA Analysis in Medicine
    15 Gene Cloning and DNA Analysis in Agriculture
    16 Gene Cloning and DNA Analysis in Forensic Science
    and Archaeology (not incuded)

    16 chapters, ~320 pages ~ 20 pages per week

    https://www.wiley.com/en-pt/Gene+Cloning+and+DNA+Analysis%3A+An+Introduction%2C+7th+Edition-p-9781119072546
    TP classes (Mostly Bioinformatics)

    ● In silico sub-cloning, replicating a cloning


    strategy on your computer
    ● PCR primer design
    ● PCR simulation
    ● Gibson assembly and homologous recombination
    ● Design CRISPR Cas9 guide RNAs
    ● Restriction mapping
    ● Sequence search and comparison by BLAST
    Evaluation

    T and TP classes are not mandatory


    (but I will keep statistics on presence)
    Theoretical Test 1 & 3
    Test 2 (practical bioinformatics)
    Computers for TPs

    You *need* a laptop for all TPs.


    (Tablet will not work)

    Who has Windows?

    Anyone has Mac?

    Anyone has Linux?


    Live quiz!

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