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Microbiology Practical

Microbiology practical Shrikhand Django subsidies Bhosda Suicide hu uh no d Django roadways bus ? to nai ke hela ki cancel karbi hai kya aap ko nai w Djd HV jdoddloidhdbdudb jdidhdbiddb

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90 views30 pages

Microbiology Practical

Microbiology practical Shrikhand Django subsidies Bhosda Suicide hu uh no d Django roadways bus ? to nai ke hela ki cancel karbi hai kya aap ko nai w Djd HV jdoddloidhdbdudb jdidhdbiddb

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Bhupesh Sahu
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Experiment No, Date PREPARATI Aim > To PEP are tr frinephe > Nuwdint agac fu anott ; dp @ctart Moditunm Lor Aho Bneent purport. 9¢ fe the micteient ey Nios -fiedk ee Sho "GAA of aga hd agax iy thu otmodtiury Ath ps do antidipy & mdiun, Que *% WW anka peniuteney Gre method AGwer a geod deter fer 7 une UE wg of bactouum Or & poli aLutpoce » Requixemonts > ty Clodan amar pork @ Rutiiad, wate. % Me OAL a yu nose | on bataner 5 paper 6) Non- absexbant “9eD ® Atrurrfkinisim fou ) py ometir NS B sabe, OFM “Beth Reiect > foptona® + S7/- RutLued, wate X 100 mt Agax mei Sl - H—- $2 Natl—Segm 37, Procedure Sea H AL the hous w9dKes area wathedt cutth wotiled wolie and dhe foot atextlized by hot abe oven. The exart wet — tthe mucbdien Loner. Maser fra Se disetued ty diated Wate - The ka.. amount af agar ke Bien oo the medium and® dinatved et ef aac es mode uf | i thy mag erircech VALAMe - ii CENORENS TERRE OR Hionecien SG HENCE 8, GUNUPUR j ms OF. IND, » BIO ror ea ¥ Experiment Date nent D206 medi fa Kheckook bi the lay conieat flack, wu qe wthh Man — pabsobint Sie ae Coneud — UwiHL quteelouwe at urd Ram paratuns . GANDEIINSEEARQOP mere BIOLOGICAnS Gi GIENCES| Guy: ES, CONGR we: OPEL Es, IND, BIOTE BIOTEGHNOROGE Page No......A Experiment No... 3 g q 1 PREPARATION OF CULTURE Memra . CNoq RTENT BRoTH ) 3 him + To ~propare the oulturte media feirekphe > Nutbuint broth Gy che bacdu of mut omodiun used “in tthe atudy oh Mijpounk ype shomicxo-exganiuny - at] qupplrs the Guerttal nivbiient ushiich oe ree quixced pur tthe bo sterial Geren BH wortotu both aac and aise Pruibefinte = ashich axe meade te baetoctoll groueh ers | | AE , oun bth «Sune ot | tthe i poxctonte Mgptic amaduinny ( bo oth)! ede fer bocterioLegicaL pLucpoars - eqoeome nts S I) Ehren flauk, - P QuittLeol es age thats — Roll a Rott nods /G tes /1a/t x GaNDATIN oo STTUIBOP §), GUNUPUR S D} BIOTECHNOLOGY, Pegs Moe eersvecssreoe Lg) Nen abiercbent etten @ AlLuminiuem Pour 1s) pr em | Me ai ees eampayiti wal es) Beef Qx beet 237: | (2) foptone 4 CT. G) QUAL watve = leone - ay PH it 2 Proordivin a, wares ate Uathed webb Experiment Mc Date vmnldaicd. Br, pH of the mediun Ss ctheckod by , paper : Ie) LONLeoL FAQtk, dU abu bent — ettten alum tn tung fou Wy Madi Us Qtertined by autecdating at IS pet puns fay’e KLmp Cotte wth, Men - woth, ! Purcarction, = OTe moduuy ehowtd& ke adguuted To suctote ithe paver ee api , wote. and aix feqtaute > Nuwuent agar ore dininy , Oupplied Sateen 7 d “ wali. Qomple Wr Olt anmple oc aol a ‘ > as Aom ple 9» Nownol aatine aalucion 5 prio “ah, -plppettu , ditittid wate , conteat flak > ATRL AyUNdr » venthons “t-e V of ce > Nej- Abs@Xrkont , AlLumintim fot - V Prin orple > a Or nectucoU. anviocanmunt ae Lau | ond comp Len populocinn a anfAng ee nim are prusent - To attdy che Vhorncteoutie ef an dndtvtolral @peciu) Jinor @pects must ib AL Prater po| | UL Ane otha apactes CET oir: Je J mut te Gelatrd Tn pure eullline University Roll No. College Roll No. Candidate's ignature CHNOLOGY One prom vouthute ef on oxgorur Ge ebtatnad ,it con be Pruwured ond Pi motatred vin O veultrc, eolLietion - Th pure PAULI ae eontain wo aingle eet ot Antex ergo » Mqny Lif owantial mickebiol aperies naxrmally px pnt WW | asl sd and wotic Orpue ee P] istote vw Cingte aprciur of ogo rum prom notwab env beooment ce on 2 (4) Are ptioaLu 5 te nub ag ox plo. } oe prparcad . () Thon, the plotu ore Oxpored to chine p “Ratre aie fox 04 fou (8 The capped, agar ploctus axe Sy etibatiol el qt s4c hor aq fou | boreyation s- Aitberonk vole niy of. anilexo org anwar | M4 Appeared on Ary Qurefoce of agan | a Patyad axghcny C py odaies ca i Mlonoceanemeceee | DEPT, CABSGIENGES,GONUPER q , |: DEPT, OFM Sc; IND, BIOTECHNOLOGY Page No oe Experiment. No. Date nreDit 008 | plot - Fou pure ULL, aie gle. cantare Gs Dranohered wth the shetp of “wnocutotitha eer do ey Prepared Ago ploue d oe 4 Sh cubation at 37° ¢ | Taptation trom wot. parraner acee Ras Procodwer - aes . a () The extol ditrtiay and upto lo ° ef the aupplied, aample & preapaxad vat aay eerent det die - 2) The ailucted eam ples axe prepared ke f0', 10-2 -t0~F hu lOm4, 1055 act 1Onee Thr aAtotiled nuwbcuant plate axe Gnecutaten? woth 1 mi of a@omple teem | Loch AL LutLey ‘ University Roll Nowe 2 {Bc Pel Oc candidate’s nature | VAE Mh orMaAkont ahourd dutiinek colony a opecias ARiexo 6 0 b op 4 TRL MUON) arngte eoleny ds Bayabowo 34° for a4 tua Quo atten qe am AL Pascadiel” > fi) Quotved 4 Ym oh aupphied la) Then, sone appount “wat in the awtfpoce The agar plote qa plate of Righout Jute J Fox preou Autturce Mhalp akocite @peciss of vies acqarbi9 « “ue cuLLactin doop to to ALY, ae plore : Aa a &y vkotion at nv Ag Gnu leo mL ef Woiad solie + diturtion Ott prope moxmoat @alene Antutioy - 5) Then , ow the Amb Of ample & taken each, divution and Plaked evr a} Aub ant Agar medium. The plotas are Gnourbactool oF DILL. 6 nk Sh the pri paxad BFC for J ike f cu ain ’ indidate s Signature eae. GaNDATIN: i BIOLOGICARS DEPT, Pe OF it MSc, ffs BoTEOHNLOSY i Experiment No. Date Port 4 hawu dy wn Inertbotan . : ; Qburvotions > The oe phates of ip ae yond LULL | ahewe AL inate seolonis of Alppercent os @ mLexo = exgantumy . Fe p apse + “g' ener Aton “ AUER woe aknghe ; wth Be Katp of a phate OD CeCe to an fon) Pies Plots fotrowed by doer Uneikotisy or 37° for 24 hu. eo Candidate’s Signature University Rott Noni. dL »cllege Roll We 06 4.185 [2B 13 | CULTURE : PLATE METHop At : AUD > To cerry ob muse een ee a Perr plote motnod, - qd Materials ere (H) 2 ie —7ABbG cubduante bx Kth epee OF £- eat LOMixed LUI, of ALCO ExHOian, 8) Nucte? orct re Plots * a) Bnecilating ekgop - I'S) Bunarn buveriere lc) QH ax Be vets | Prnerdweor > ee (4) Pro posxed che nubaent an Alea. Psdyedascehin GaNDAT, a eee eee SEP: GF. IND, homer = NO wi Panne Date 22.1, Powe Proce Method > PR RES 1:0 mb bite ino, Fmbtt Heo FL 1.0 ami Aone Col giuten ‘18 dilution) Cio dulutior var) Ziro mk tt J worn 090% GANDA moneciea |: BEPE mn > OG co Qach jPebciplate + ) ALLoWwed tho modium to @nlidi a MB icibadad cthy Gnecutoctad phates oe loa - 48 hy at 376 tu Sup wesc dl pecction- Obawu ows Fan tee the Plots for Apprreance of fn die) clu secon ee ee baough -eut the oe omadtitum > | Rea 5) O+ WEL be ebserurd thot opel pourced plots gut Poe dado and Kee number _ Gf Gelontuy whieh wUb be dibeuibused 5 mow ox los Apaxnely ‘y due plat which may be trop apored (aus- wuturnd) to othe ane dio , Prucoution > DDD frurh etude pipette for cach GANDETINS Tue EIOBOGIERS Se Te mointes pgonums by abu plote MAthEd rLals Agen Say Ritu watre Cy ONS bolonce Wy) Rance Lok - Measuring oy lirdar 2 N Q . (vi) Giles aed [magnatic cotton Ai) Non - abs ewant (viti) Autoclave tix) Potuidinh (4) OnnecuLoting pep cxiy Bank las 20d (xii) Lamina ax flow (xtti) Qnerbotor are _0& College Roll No. : GAND MINS TTB | meme, one SGIENGES DEPT, OFM 6s; Np, imoTEGHNOROGy Experiment to, | Date ok by gee dxatation cond andr soci §AuLtuy a ; eg ae gad, plate mMotye By uiing dows Qn Lec g eon wolats , ff Actes Sears to the: ernie | ge methecds hath fe acc ieling ns Aaa i ° Tene D nl bealanies thot eure cho Savetay aingt, Calany @n.gkhe, petidis plotes)- OF the, diverdity of ids mere @ the Yrontt at amc awe dorge, dn this Lose che have haon wapeated co Sasalote ppectes of qeiier@ or gantry Pan mined, ors “FT "OPN oLrCOLLD the ergs Mia Cudture , che amceo PHAN OVEA to age plotes diephrcal ~ May cathy he KUb a an Sp necuboting flstes Jeep: Pox Uiu deh rgyre , Rath the dachniqy 9s GR OX methods are ep : Aba strane} safe. the orn ee Ona uthey aco Jpnocutated act approprrals ate unsice BOD fneurboler nature xa Fe eae pasoen ness GaNpar; OG)! wooden ‘ DEPT, OF ne CENGES GNI Ee sma At s IND, BIOTEGHNOBE i . Ho. : pi Experiment No.....LX. froceout Yb (Lprrod plots ott 4 1.2 fyi md Of Atoite volratined op pendory cube, ct token a of MUcKo- Oantiny to fas) Qhoked them and y QDWHOD = Than, tne agar platy axe propa ~xad by fouarseng the Oreaptic eonditions | iii) too mL of mintokial aatutien SG beans | eocod ho Agar .plocteGinwide cths ace foi flow os the holp eb» bend gla vied , the Gomple 4s eurny ilstuduted ever the agar pile ky ant acting the pebuidivh - a Ki) Then, the courte is Qn cubatrad of 37 0x a4 Rowe _— Ane ort of micro ergantums Prerautions * Ree EMS a & MO A akeps ahawta ke OX aur . Ue phy pouse cto ONPVUMLNT en Wgnt of eminase abe flew 0% S mina, bate tduchey Mn dnverted iD a tho prbudis o Arsh pee in cuboton - PeatLon candida, ow worter, Tin con dane Loophat Dhol do Khe micx bio! 2, Uitersty non no 22102... Solege Rott no..4 LGLBLALBLS g 5 manatee scien SSSR i DEPE GP Sa, EMS HO u experlment No, SPREAD PLATE METHOn Date 2g ra: Sept Palppetted a. AMO ample onto the cere #4 an anor medium plots ¢ = Sapa: Atnped 0 Gus aprcoodex Walp haoke of ethane ? \ Sctha nal: @sokid oot - Siro: Bdepied {Lamune Aprccador and atlowed tr to - : cee Atp4 2 aprended ne Qonple evenly ever He o apace ustth the prvi, oud. Ure Tuy Candidate's ‘Signature Ii) The genus St amniexo ergo ue Qhaked the omntrox 4 d mic wat + i) By au swing MLO — OXLY-AM tain aed provieruty Kit) The prota oh ht tokiig ey Atnotght Sine felroured. by” eus (9 The surces Hilal Hoe Fgh ds éanTinn > the atabs we a ob 2 put, met kowket ur valierked Hb - : ee PR euro 4) HED Gero. BX Prcesosatten Experiment Ho, Date vs [1.0 Preparation of Qount The SCY LLUCe of Volume ef Cts LUKE me diun P Aube at Gy the, plugged Coxcefecrty ond ~ Gicue I auteclave - : i ae ws AaRen un MANTEL tg + Abt tut Lixetion yrs “ibe carton ing Moditim axe page 5 Aeartin, ot 36 4 ate Tho the {Loe - @e, as “to ublLurd MaAxtMlLLy dament of Proittz ontot pox With aut a tno Rua "The cmodtium 4¢ the atlawed to cool and CELA} E Ab tox artidi}uation , the @lants are Wouretid and Aud Uy gadrt gerrarer foleng With dote eard and indicating tHe molibe ¢ the Level modu acoso Oo Tr ‘Candidate's Sfynature Universi z : IY Roll NowmadelPrzind ” Ci Oona dala cl ald Bee aiia. 06 LQ Experiment. No, Te. Date .WtA.2.1: 9. STAINING i Bacterta BY g —_—~_ METHop > . MM > To Atidy the pr ax im oF Loy of bactorta | = 8 ee hinerple + The scam GLOUN ING “uO wupporential atacr tng Method OLecLaped by Rr Hans Chl Soper Gram, Dartuh me ° Prytotan Jo the Veon Sie ges be DEST og ed tobe Baining mesbec foe ddan tying ara Roloc frying boctea, ca awe groups U) Goeam ve) - HGS Gyrcaen (ve) The bacteria, Whitey vitlain cthe prtmas Moin (appooxrs dork he ox wiolet) dee Met cdocelewixocd Uhan atoun hy Upon’ emathed axe cotteol wor gra re, whoroos here Upec eee YOU tat Woe eptour and Crue ator yy Anporarsy (appro sed or per) Experiment No, Date 10" [+0 Roquuuiter “pero lb pecaoke ia) Lay cto k Vivo tint Sotution 4 9 MOULCLOL Vue Ethanet Setution B : $ Loy —2..Atomenin oxalate Outtil, usoliy — 2 See MOU Ey DEL ety A+ Qe uctionn 1D Grardo Lediine (Modarant) — Ki { 9 got Di gi Lied waltx (300 me T Sedine (44m) ec) Exnanel, = 9s */. ethanet (a) Sajpscoinine as Safsconinre eotirtisn (0-2rgr) + Etpanel Come) + Mutrued water (40 mL) MY) Od veuttrrxre of backocia M4) Onn ac wbactin p Mii) Bunarn by Experiment. No, i; Date 00° [189 dy) QO peu ib y Glan Aldo Vi) Blotting Peper dure Sees Thin amor of hacks PUpoid Wy oGendhue auvtforce 4 stab ak 6 z s Muide Us ain dcred and ax are Acat tixed on tre abiae V bx The alide wi atninod w0th veut Violet ro tution feu 4 ann 2 J ae Vv The @lide ds washed by chap woke The usdine olution (modexant) v apphied v Zee Again , wahed woud top wat Tk odonahu' u r j (eounte atoning) fox 4 atin & Top Watin and ate Asrde i The attain amecce of QKarrinod — onder Artouning whieh ftve) wand ushicw du |} Cbasswatton : ee Tre atlide “Us the Kells Dee the AOfJHOMUENE aX aruneck oted Mera aerpe aca Hotained (efnk cote) University Rot College Roll No, Alide Us Deeoloue De sue cLdLing Udbuop wus oF ipa s: : ¢ e ) the tikes Bid Meu OL hy dna, pe vepke b k bern ston Oved (4o-c J > conds rd ws A totth tap — cwacter Zia Than, dk dy qfleedsd wit) aolfons qLamM undue at prot colour "xperiment No.2 Da | $ Reade : - ate Rw Aw tho AULS Lory Peet oksote okt cha d > hay ace ‘ *8d~ ahaped + Qo bac utuy LAS A0) xonin, OnxXe mening Ly ANG RLU, (corrto @toin) alt bea purROe 5 cthey Oy Gorm ()ve ty natine one LusLan g RR, Qo, the Guppled @ample contain Guam (-ve) ha cis, - Precaution : pee (cr) AL usa Use frrth cond young cute | (tes than 24 tou) to averd cu hading D bx o Raoting auouun bis aucenee Bei tans Pexoctian : | ® voc decetarvtixatiey of dhe mo hour be avetded: e be thy and uniform . eS a) BIOBOTICAL SCENES Coauem j a: DEPE, GFI Se; In, HIOTECHNOROGE No «1.4.2.4 Experiment le Date seml® 2 Unversity Roll No Oi : cia

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