‫ ص‬11:15 2023/‫‏‬4/‫‏‬15 500+ Chromatography MCQ and Answer with FREE PDF – Question and Answers

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500+ Chromatography MCQ and Answer with

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BY PULAKESH NATH on NOVEMBER 6, 2021

These Chromatography MCQ and Answer are most important for Biochemistry,
B.Sc/M.Sc/Phd Botany/Chemistry, Biology, Chemistry. These Chromatography MCQ
and Answer helps you to improve your conceptual insight on the phase system.
You can also buy the best book on Chromatography in cheap price.

Chromatography MCQ and Answer

These Chromatography MCQs are based on the following topics:

50+ MCQ On Paper Chromatography with FREE PDF

50+ Gas Chromatography MCQ with FREE PDF

50+ MCQ On Ion Exchange Chromatography with FREE PDF

50+ MCQ On Thin Layer Chromatography with FREE PDF

50+ MCQ On HPLC with Answer and FREE PDF

50+ MCQ On Column Chromatography with FREE PDF

50+ MCQ On Gel Filtration Chromatography with FREE PDF

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50+ MCQ On Flash Chromatography with FREE PDF

50+ MCQ On Size Exclusion Chromatography with FREE PDF

50+ Partition Chromatography MCQ with FREE PDF

50+ Affinity Chromatography MCQ with FREE PDF

Chromatography MCQ and Answer

1. In chromatography, which of the following can the mobile
phase be made of?

a) Solid or liquid

b) Liquid or gas

c) Gas only

d) Liquid only

Answer: b

2. Which of the following cannot be used as an adsorbent in

Column adsorption chromatography?

a) Magnesium oxide

b) Silica gel

c) Activated alumina

d) Potassium permanganate

Answer: d

3. Which of the following types of chromatography involves the

separation of substances in a mixture over a 0.2mm thick layer
of an adsorbent?
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a) Gas liquid

b) Column

c) Thin layer

d) Paper

Answer: c

4. In Column chromatography, the stationary phase is made of

_________ and the mobile phase is made of _________

a) Solid, liquid

b) Liquid, liquid

c) Liquid, gas

d) Solid, gas

Answer: a

5. In Thin layer chromatography, the stationary phase is made of

_________ and the mobile phase is made of _________

a) Solid, liquid

b) Liquid, liquid

c) Liquid, gas

d) Solid, gas

Answer: a

6. In which of the following type of paper, chromatography does

the mobile phase move horizontally over a circular sheet of
paper?

a) Ascending paper chromatography

b) Descending paper chromatography

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c) Radial paper chromatography

d) Ascending – descending chromatography

Answer: c

7. Liquid chromatography can be performed in which of the

following ways?

a) Only in columns

b) Only on plane surfaces

c) Either in columns or on plane surfaces

d) Neither in columns nor on plane surfaces

Answer: c

8. Gas chromatography can be performed in which of the

following ways?

a) Only in columns

b) Only on plane surfaces

c) Either in columns or on plane surfaces

d) Neither in columns nor on plane surfaces

Answer: a

9. In Gas-liquid phase chromatography, the stationary phase is

composed of _________ and the mobile phase is made of
_________

a) Solid, liquid

b) Liquid, liquid

c) Liquid, gas

d) Solid, gas

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Answer: c

10. Which of the following types of chromatography involves the

process, where the mobile phase moves through the stationary
phase by the influence of gravity or capillary action?

a) Column Chromatography

b) High Pressure Liquid Chromatography

c) Gas Chromatography

d) Planar Chromatography

Answer: d

11. Chromatography is a physical method that is used to separate

and analyse __________

a) Simple mixtures

b) Complex mixtures

c) Viscous mixtures

d) Metals

Answer: b

12. In which type of chromatography, the stationary phase held

in a narrow tube and the mobile phase is forced through it under
pressure?

a) Column chromatography

b) Planar chromatography

c) Liquid chromatography

d) Gas chromatography

Answer: a

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13. In chromatography, the stationary phase can be ___________

supported on a solid.

a) Solid or liquid

b) Liquid or gas

c) Solid only

d) Liquid only

Answer: a

14. Using Chromatogram as detector in Chromatography, a graph

is obtained between ____________ and time.

a) Quantity

b) Density

c) Concentration

d) Specific gravity

Answer: c

15. In older analytical methods, which of the following methods

were used to allow movement of the mobile phase?

a) Pumps

b) Pressure

c) Gravity

d) Blowing air into the column

Answer: c

16. Which of the following steps takes place after injection of

feed in Column chromatography?

a) Detection of components

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b) Separation in the column

c) Elution from the column

d) Collection of eluted component

Answer: b

17. What happens during the ‘elution from the column’ phase in
chromatography?

a) Components with greatest affinity elute first

b) Components with least affinity elute first

c) Components elute in a random manner

d) Components elute according to their concentration in the mixture

Answer: b

18. In chromatogram, the position of peaks on the time axis can

be used to determine which of the following?

a) Components of the sample

b) Amount of component in the sample

c) Column efficiency

d) Column resolution

Answer: a

19. In chromatogram, the area under the peak can be used to

determine which of the following?

a) Components of the sample

b) Amount of component in the sample

c) Column efficiency

d) Column resolution
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Answer: b

20. What are the uses of partition chromatography?

a) Separation of amino acids

b) Analysis of closely related aliphatic alcohols

c) Separation of sugar derivatives

d) All of the mentioned

Answer: d

21. Which type of chromatography is used for the structural

analysis?

a) Column chromatography

b) Paper chromatography

c) Partition chromatography

d) Affinity chromatography

Answer: b

22. Which of the following is not done using column

chromatography?

a) The identification of unknown compounds

b) The determination of homogeneity of chemical substances

c) Separation of inorganic cations or complexes

d) Separation of geometric isomers

Answer: c

23. Affinity chromatography is used for the analysis and isolation

of ____________

a) Insoluble starch substances

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b) Enzyme tyrosinase

c) Antibodies bound with a covalently-attached antigen on cellulose column

d) All of the mentioned

Answer: d

25. Purification of enzymes and proteins is done using

____________

a) Affinity chromatography

b) Liquid chromatography

c) Column chromatography

d) Thin layer chromatography

Answer: a

25. Which of the following is not an application of high

performance liquid chromatography?

a) Analysis of proteins, drugs and explosives

b) Separation of pharmaceutical drugs

c) Elimination of undesirable substances from blood

d) Separation of lipids, fatty acids and steroids

26. The analysis and separation of industrial products like soap

and synthetic detergents is done using ____________

a) Thin layer chromatography

b) Gas chromatography

c) Ion exchange chromatography

d) Partition chromatography

Answer: b
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27. Which of the following is the application of ion exchange

chromatography?

a) The softening of hard water

b) The demineralisation of water

c) The separation and determination of anions

d) All of the mentioned

Answer: d

28. The quantitative analysis is done using ____________

a) Ion exchange chromatography

b) Thin layer chromatography

c) Gas chromatography

d) Liquid chromatography

Answer: c

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FAQs on Chromatography
Chromatography are made of
What are chromatography techniques?
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What are chromatography columns?
What are chromatography resins?
What are chromatography used for?
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Can chromatography separate drugs from blood?
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