Histopathology Test Master List

SRI NATHELLA SAMPATHU CHETTY CLINICAL LABORATORY
(UNIT OF MEDICAL RESEARCH FOUNDATION) HISTOPATHOLOGY

TEST MASTER LIST
HISTOPATHOLOGY
Tests (Specimens) under Scope of NABL
Code No. Specimen Turn over Time Fixative Used/Acceptance Criteria

501A Eyeball 4 DAYS 10%Neutral buffered formalin
502A Exenterated specimen 4 DAYS 10%Neutral buffered formalin

503A Eviscerated specimen 4 DAYS 10%Neutral buffered formalin
504A Corneal button 4 DAYS 10%Neutral buffered formalin
505A Epiretinal membrane 4 DAYS 10%Neutral buffered formalin
506A Sub retinal membrane 4 DAYS 10%Neutral buffered formalin
507A Biopsy-lid 4 DAYS 10%Neutral buffered formalin
508A Biopsy -Conjunctiva 4 DAYS 10%Neutral buffered formalin
509A Biopsy -Cornea 4 DAYS 10%Neutral buffered formalin
510A Biopsy- Orbit 4 DAYS 10%Neutral buffered formalin
511A Iris tissue 4 DAYS 10%Neutral buffered formalin
550 Others-Tissue and cell 4 DAYS 10%Neutral buffered formalin
block of fluids
501B- Frozen section 30 MINUTES Fresh unfixed tissue
511B
524- Amyloid stain 1 DAY Paraffin block/Paraffin sections
525 Mucin stain 1 DAY Paraffin block/ Paraffin sections
526 Collagen stain 1 DAY Paraffin block/ Paraffin sections
528 GMS stain 1 DAY Paraffin block/ Paraffin sections
Issue No: 1 Issue Date: 11.12.12 Amend No: 0 Page 1 of 6

Prepared and Issued By: Reviewed And Approved By:
Head of the Department : Dr.S.Krishna Kumar Management Representative : Dr.S.B.Vasanthi
TEST MASTER LIST
529 Gram stain 1 DAY Paraffin block /Paraffin sections

530 Calcium stain 1 DAY Paraffin block/ Paraffin sections
531 AFB stain 1 DAY Paraffin block/ Paraffin sections
532 Oil O’red stain 1 DAY Paraffin block/ Paraffin sections
533 Perls stain 1 DAY Paraffin block/ Paraffin sections
537 Small sized specimen 4 DAYS 10%Neutral buffered formalin
(3mm-5mm)
538 Medium Sized specimens 4 DAYS 10%Neutral buffered formalin
(10mm)
539 Large sized specimens 4 DAYS 10%Neutral buffered formalin
(>20mm)

TEST MASTER LIST
CYTOPATHOLOGY
Tests (Specimens) under Scope of NABL

512 Aqueous aspirate 3 DAYS
513 Vitreous aspirate 3 DAYS Unfixed sample/smears
514 Sub retinal fluid 3 DAYS Unfixed sample/smears
515 Smear cytology-lid 3 DAYS Unfixed sample/smears
516 Smear cytology- 3 DAYS Unfixed sample/smears
Conjunctiva
517 Smear cytology-cornea 3 DAYS Unfixed sample/smears
IOL Cytology 3 DAYS 70%or 95% Alcohol
519 FNAC 3 DAYS Unfixed sample/smears
524- Amyloid stain 1 DAY Unfixed sample/smears

525 Mucin stain 1 DAY Unfixed sample/smears
526 Collagen stain 1 DAY Unfixed sample/smears
528 GMS stain 1 DAY Unfixed sample/smears
529 Gram stain 1 DAY Unfixed sample/smears
530 Calcium stain 1 DAY Unfixed sample/smears
531 AFB stain 1 DAY Unfixed sample/smears
532 Oil O’red stain 1 DAY Unfixed sample/smears
533 Perls stain 1 DAY Unfixed sample/smears

TEST MASTER LIST
Tests (Non Ocular Specimens) not under the scope of NABL
Code Specimen Turn over Time Fixative Used/Acceptance Criteria

No
537 Small sized-Skin biopsies, Lymph 4 DAYS 10%Neutral buffered formalin
Node biopsy, Any other tissue
measuring 3mm- 5mm
538 Medium sized Skin biopsies, 4 DAYS 10%Neutral buffered formalin
Lymph Node biopsy, Any other
tissue measuring 10mm
539 Large sized Skin biopsies, Lymph 4 DAYS 10%Neutral buffered formalin
Node biopsy, Any other tissue
measuring >20mm
522 Aspirates-CSF,Pleural fluid, 3 DAYS Unfixed sample/smears
Bronchial alveolar lavage and any
other body fluids
524- All special stains 1 DAY Paraffin block, Unfixed sample/smears
536
537B- Frozen section 30 MINUTES Unfixed sample
539B
Specimens for histopathology and cytopathology are collected by the surgeons at operation theater/out patient department
specimens are directly received in person or by mail at SNSC lab-Histopathology 3rd floor New Building

TEST MASTER LIST
THINGS TO BE DONE WHILE SENDING OCULAR PATHOLOGY SPECIMENS
Proper labelling of the specimen (i.e. it should contain the following information)
a. Name of the patient

b. Age of the patient
c. The type of material
d. The eye
e. Date and time of collection
f. Doctor’s name
2. Adequate clinical summary and clinical diagnosis
3. Proper fixation in proper preservative
PRESERVATION OF THE SPECIMEN
• Tissue should be preserved immediately in the proper fixative as drying of the tissue causes artifacts.
• For routine surgical specimens 10% neutral buffered formalin is used, 70% alcohol for IOL’s,
• Approximate volume of the fixative required for different kinds of specimens are
a. Cornea - 5 to 10 ml
b. Eyeball - 150 to 200 ml
c. Exenterated specimen - 500 ml
d. Other biopsy material - according to the size of the specimen

TEST MASTER LIST
MAILING OF SPECIMENS:
The specimen collected should be placed in suitable sized containers (preferably plastic container), with adequate fixative. The
container should bear the label with details like Name, Age/sex of the patient, specimen, etc. The lid of the container should be sealed
with help of adhesive tape to prevent leaking of the fixative.
The specimen should be mailed to Sri Nathella Sampathu Chetty Clinical Laboratory –Histopathology Laboratory,3rd Floor, New
Building, old no:18,New No:41,College Road, Nungambakkam,Chennai-600 006 with proper requisition from the surgeon providing
the required details mentioned above along with the address for communication. (E-mail/ telephone no. /Fax). The charges intimated
to be sent as Demand Draft payable to Medical Research Foundation.
Acceptance and rejection Criteria
S. No. Acceptance Criteria

Rejection Criteria
1. Sample fixed in Formalin will be Sample not fixed in formalin will be
accepted rejected
2. Paraffin block , stained No clinical information will be rejected

sections/smears will be accepted
3. Unstained sections /Smears will Sample less than 1 mm will be rejected

be accepted
Note: If the specimens are sent for both microbiological as well as histopathological investigation, the specimen would be received in
unfixed condition.


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SRI NATHELLA SAMPATHU CHETTY CLINICAL LABORATORY

(UNIT OF MEDICAL RESEARCH FOUNDATION) HISTOPATHOLOGY

Code No. Specimen Turn over Time Fixative Used/Acceptance Criteria

502A Exenterated specimen 4 DAYS 10%Neutral buffered formalin

Issue No: 1 Issue Date: 11.12.12 Amend No: 0 Page 1 of 6

Code No. Specimen Turn over Time Fixative Used/Acceptance Criteria

529 Gram stain 1 DAY Paraffin block /Paraffin sections

Issue No: 1 Issue Date: 11.12.12 Amend No: 0 Page 2 of 6

Code No. Specimen Turn over Time Fixative Used/Acceptance Criteria

524- Amyloid stain 1 DAY Unfixed sample/smears

Issue No: 1 Issue Date: 11.12.12 Amend No: 0 Page 3 of 6

Tests (Non Ocular Specimens) not under the scope of NABL

Code Specimen Turn over Time Fixative Used/Acceptance Criteria

Issue No: 1 Issue Date: 11.12.12 Amend No: 0 Page 4 of 6

THINGS TO BE DONE WHILE SENDING OCULAR PATHOLOGY SPECIMENS

a. Name of the patient

2. Adequate clinical summary and clinical diagnosis

3. Proper fixation in proper preservative

PRESERVATION OF THE SPECIMEN

Issue No: 1 Issue Date: 11.12.12 Amend No: 0 Page 5 of 6

Acceptance and rejection Criteria

S. No. Acceptance Criteria

2. Paraffin block , stained No clinical information will be rejected

3. Unstained sections /Smears will Sample less than 1 mm will be rejected

Issue No: 1 Issue Date: 11.12.12 Amend No: 0 Page 6 of 6

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