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    Onion Cell - LAB 5 REPORT

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    edaviddavis9
    This lab report details an experiment measuring the water potential of onion cells when exposed to different sucrose solutions. Onion epidermis was placed in sucrose solutions ranging from 0.2M to 1.0M, as well as distilled water. The number of plasmolyzed cells in each sample was counted after 20 minutes. Higher sucrose concentrations resulted in more plasmolysis, from 4% at 0.2M to 100% at 1.0M. Based on the relationship between sucrose concentration and plasmolysis, the water potential was calculated for each solution. The water potential became more negative with increasing sucrose concentration. The molarity at 50% plasmolysis, indicating incipient plasmolysis, was

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    Onion Cell - LAB 5 REPORT

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    edaviddavis9
    605 views8 pages
    This lab report details an experiment measuring the water potential of onion cells when exposed to different sucrose solutions. Onion epidermis was placed in sucrose solutions ranging from 0.2M to 1.0M, as well as distilled water. The number of plasmolyzed cells in each sample was counted after 20 minutes. Higher sucrose concentrations resulted in more plasmolysis, from 4% at 0.2M to 100% at 1.0M. Based on the relationship between sucrose concentration and plasmolysis, the water potential was calculated for each solution. The water potential became more negative with increasing sucrose concentration. The molarity at 50% plasmolysis, indicating incipient plasmolysis, was

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    Onion cell - LAB 5 REPORT

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    This lab report details an experiment measuring the water potential of onion cells when exposed to different sucrose solutions. Onion epidermis was placed in sucrose solutions ranging from 0.2M to 1.0M, as well as distilled water. The number of plasmolyzed cells in each sample was counted after 20 minutes. Higher sucrose concentrations resulted in more plasmolysis, from 4% at 0.2M to 100% at 1.0M. Based on the relationship between sucrose concentration and plasmolysis, the water potential was calculated for each solution. The water potential became more negative with increasing sucrose concentration. The molarity at 50% plasmolysis, indicating incipient plasmolysis, was

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    © All Rights Reserved
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    Download as pdf or txt
    605 views8 pages

    Onion Cell - LAB 5 REPORT

    Uploaded by

    edaviddavis9
    This lab report details an experiment measuring the water potential of onion cells when exposed to different sucrose solutions. Onion epidermis was placed in sucrose solutions ranging from 0.2M to 1.0M, as well as distilled water. The number of plasmolyzed cells in each sample was counted after 20 minutes. Higher sucrose concentrations resulted in more plasmolysis, from 4% at 0.2M to 100% at 1.0M. Based on the relationship between sucrose concentration and plasmolysis, the water potential was calculated for each solution. The water potential became more negative with increasing sucrose concentration. The molarity at 50% plasmolysis, indicating incipient plasmolysis, was

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    LAB NO: 5

    DATE: October 18th, 2023

    TITLE: Water Potential of an Onion Cell

    OBJECTIVE: To measure the water potential of onion cells

    MATERIALS:

    ● 6 microscope slides Forceps

    ● Onion epidermis Cover slips

    ● Range of sucrose solutions from 0.2M to 1.0M Mounted needle

    ● Distilled water

    ● Microscope

    PROCEDURES:

    1. The slides were labelled 0.2M, 0.4M, 0.6M, 0.8M, 1.0M, 0.0M (distilled water)

    2. A piece of onion scale leaf was collected and the epidermis was removed from it.

    3. The epidermis was cut into 6 equal pieces (about 1cm square).

    4. A piece of the epidermis was put onto each of the slides.

    5. 3 drops of the appropriate solution were added to each slide, a coverslip was added and the

    time was noted.

    6. The slides were allowed to stand for 20 minutes.

    7. A table was drawn for the results.

    8. For each slide, 50 cells were counted and the plasmolyzed cells were recorded.

    9. A graph of the percentage plasmolyzed was plotted.


    RESULTS:

    Sucrose Amount of Cells Percent Water Potential


    Concentration (M) Plasmolysed Plasmolysis (%)

    0.0 0/50 0 0

    0.2 2/50 4 -5

    0.4 4/50 8 -10

    0.6 27/50 54 -15

    0.8 38/50 76 -20

    1.0 50/50 100 -25

    THE TABLE SHOWING THE AMOUNT OF PLASMOLYSED CELLS, PERCENT

    PLASMOLYSIS AND WATER POTENTIAL FOR ONION CELLS WITH DIFFERENT

    SUCROSE CONCENTRATIONS

    PERCENT OF PLASMOLYSED CELLS VS MOLARITY SUCROSE SOLUTION


    Calculations:

    Percent of cells plasmolysed:

    1. 0.0

    0/50 x 100 = 0%

    2. 0.2

    2/50 x 100= 4%

    3. 0.4

    4/50 x 100 =8%

    4. 0.6

    27/50 x 100 =54%

    5. 0.8

    38/50 x 100 =76%

    6. 1.0

    50/50 x 100 =100%

    Calculate the water potential: =(-i)(c)(r)(f)

    1. 0.0

    (-1)(0)(0.0831)(300k) = 0

    2. 0.2

    (-1)(0.2)(0.0831)(300k) = -4.986

    3. 0.4

    (-1)(0.4)(0.0831)(300k) = -9.972

    4. 0.6
    (-1)(0.6)(0.0831)(300k) = -14.958

    5. 0.8

    (-1)(0.8)(0.0831)(300k) = -19.944

    6. 1.0

    (-1)(1)(0.0831)(300k) = -24.93

    Molarity at 50% plasmolysis:

    ● 0.6 m = 28 plasmolysed cells

    DISCUSSION:

    An onion cell's water potential was assessed in this lab. The statistic that depicts how

    likely it is for water to transfer from one place to another is called water potential. Only when

    there is the possibility for osmosis, which is the passage of water over a semipermeable

    membrane from a region of low water potential to a region of high water potential, is water

    potential often evaluated. The flow of water to the leaves to carry out photosynthesis requires the

    presence of water potential in plants. In the lab, it has been demonstrated that the relationship

    between water potential and solute concentration is causal: as the solute concentration rises, the

    water potential for the solution's water drops because it is less likely that the water would

    undergo osmosis. In this experiment, onion cells were exposed to sucrose solutions that had a

    greater negative water potential than the cells had, causing water to osmotically exit the cell.This

    resulted in the cell's cytoplasm moving inward and losing its ability to resemble the cell wall, a

    process known as plasmolysis. A cell becomes flaccid when a solution is hypotonic to it because

    the water inside the cell attempts to dilute the solution by osmosis. A cell will become turgid if it
    is hypertonic to a solution because the water in the solution will flow inside the cell in an effort

    to dilute the solvent there.

    Finding the solute potential was necessary before calculating the water potential. The

    osmotic potential, also known as the solute potential, measures the likelihood that osmosis will

    occur. The equation -iCRT, in which I is the ionization energy (1), C is the solution's

    concentration, R is the pressure constant (0.0831), and T is temperature, was used to compute the

    solute potential. Since the environment's temperature was 30 celsius, T was set to 300 K.

    The water potential was also found to be the same as the solute potential as the pressure

    potential was assumed to be 0. This led the water potential for 0.0M, 0.2M, 0.4M, 0.6M, 0.8M

    and 1.0M to be -0, 4.99, -9.97, -14.96, -19.94, and -24.93 respectively. It was also found that the

    plasmolysis point, which is the threshold for where plasmolysis begins, that was assumed to be

    50%, is 0.625M.

    LIMITATIONS:

    ● Onion wasn't in a good or fresh condition. (old onion)

    POSSIBLE SOURCE OF ERROR:

    ● A mix in different concentrations can cause the cell to not plasmolyze as would in the

    measured concentration. (Classmates not placing dropper in the original concentration)

    CONCLUSION:

    ● The water potential of onion cells were successfully measured and recorded, the water

    potential decreased as the sucrose solution increased and the percent plasmolysed usually

    tends to increase as the solution becomes more hypertonic. 50% of cells undergoing

    plasmolysis under an osmotic environment is referred to as incipient plasmolysis. At this


    time, the average osmotic potential inside the cell is equal to the medium's osmotic

    potential.

    REFERENCES:

    metergroup. (2022, November 17). Defining water potentialm. METER. Retrieved october

    17, 2023, from

    https://www.metergroup.com/en/meter-environment/measurement-insights/defining-water-

    potential

    precollege. (2020, October 5). Practice problems osmosis and water potential - New Jersey

    Institute of ... osmossis and Diffusion. Retrieved october 17, 2023, from

    https://www.njit.edu/precollege/sites/njit.edu.precollege/files/lcms/docs/RET_2012_Osmos

    is-Diffusion___SA_-_Water_Potential_Review__Instructor.pdf
    Lab 5 Mark Scheme

    Cells & Water Potential (Observation/Recording/Reporting)

    Report Format & Procedure (4 marks)

    - Correct spelling, grammar and punctuation [1]

    - Procedure: clear, logical sequence of steps [1]

    - Procedure: past tense/passive voice/3 rd person [1]

    - Acceptable scientific reporting, including units [1]

    Observations & Results (8 marks)

    - Describe effect of osmotic action on cells [2]

    - Accurately tabulate percentage plasmolysis [2]

    - Accurately plot percentage plasmolysis vs. molarity [2]

    - Accurately report molarity at 50% plasmolysis [1]

    - Accurately report water potential of cells [1]

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