Tamarind Extract Antibacterial Study

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Abstract

The phytochemical analysis and antibacterial activity of aqueous pulp extract of


Tamarindus indica were studied. The aqueous pulp extract of this plant was
obtained using hot water extraction method. The antibacterial activity of aqueous
pulp extract of this plant was carried out against four bacteria; Escherichia coli,
Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi by disc
diffusion method. Phytochemical constituents present in the extract were found to
include saponins (2.2%), alkaloids (4.32%) and glycosides (1.59%). Aqueous pulp
extract of Tamarindus indica showed antibacterial activity against all the tested
bacteria in the order of sensitivity as Staphylococcus aureus>Escherichia coli
Pseudomonas aeruginosa with the exception salmonella typhi. The antibacterial
activity of aqueous pulp extract on Staphylococcus aureus was sensitive at 80, 120,
140, 160 and 180 mg mL of extract with 0.2, 0.3, 0.6, 0.8 and 10.0 mm zones of
inhibition while Escherichia coli revealed 0.2, 0.2, 0.4 and 0.6 mm zones of
inhibition at 120, 140, 160 and 180 mg mL of extract, respectively. Pseudomonas
aeruginosa was only sensitive at 140, 160 and 180 mg mL of the extract with 0.4,
0.6 and 0.8 mm zones of inhibition.

Review

The study investigated the phytochemical composition and antibacterial properties


of the aqueous pulp extract obtained from Tamarindus indica (tamarind) using a
hot water extraction method. The researchers identified three key phytochemical
constituents in the extract: saponins (2.2%), alkaloids (4.32%), and glycosides
(1.59%).To assess antibacterial activity, the researchers conducted tests against
four bacterial strains: Escherichia coli, Staphylococcus aureus, Pseudomonas
aeruginosa, and Salmonella typhi. The disc diffusion method was employed to
measure inhibition zones, a common approach in antibacterial studies.

Results indicated that the aqueous pulp extract exhibited antibacterial activity
against all tested bacteria, although the degree of sensitivity varied.
Staphylococcus aureus was the most sensitive, displaying inhibition zones ranging
from 0.2 to 10.0 mm at different extract concentrations. Escherichia coli and
Pseudomonas aeruginosa showed lower sensitivity, with smaller inhibition zones
(0.2 to 0.6 mm) at specific concentrations.
While the study noted antibacterial activity against Salmonella typhi, detailed
results for this bacterium were not provided. Overall, the findings suggest that
Tamarindus indica may contain antibacterial compounds that could be explored
further for their potential applications in combating bacterial infections. Further
research is needed to understand the mechanisms of action and to determine the
specific compounds responsible for the observed antibacterial effects.

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