Scribd
Upload
158 views

Lab Manual Toad Dissection

This document provides instructions for dissecting a toad in a high school laboratory setting. It begins with an introduction explaining that toads are commonly used for dissection due to similarities between toad and human anatomy. Section II lists the necessary materials for the dissection. Section III provides detailed step-by-step instructions for preparing the toad by paralyzing its limbs, making incisions in the skin and muscles, and identifying internal organs. Identification of organs is graded by the teacher. Finally, Section IV outlines a point system for evaluating students' lab work.

Uploaded by

Degorio, Jay Anthony G. 11 - Quartz
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
158 views

Lab Manual Toad Dissection

This document provides instructions for dissecting a toad in a high school laboratory setting. It begins with an introduction explaining that toads are commonly used for dissection due to similarities between toad and human anatomy. Section II lists the necessary materials for the dissection. Section III provides detailed step-by-step instructions for preparing the toad by paralyzing its limbs, making incisions in the skin and muscles, and identifying internal organs. Identification of organs is graded by the teacher. Finally, Section IV outlines a point system for evaluating students' lab work.

Uploaded by

Degorio, Jay Anthony G. 11 - Quartz
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
You are on page 1/ 5

Names: Section:

Laboratory Manual

I. Introduction

Toads are mostly used by students in high schools for


dissection in order to demonstrate the different organ systems
of the body. This is due to the fact that the presence and
positions of the said organ systems of toads are somewhat
similar to a human being. With this, students in high schools
are able to provide insights into the internal functioning of the
human body.

II. Materials Needed

1. Dishwashing liquid
2. Sponge
3. Hand soap
4. Paper towel
5. Dissecting set
6. Blade
7. Gloves
8. Lab gown
9. Face mask
10. Toad
11. Dissecting pan

III. Methods

A. General Instruction
The following students shall not be allowed to enter the
laboratory:
1. Those who are not in proper laboratory attire;
2. Those who did not bring their specimen; and/or,
3. Those who did not bring their dissecting set.

Page 1 of 5
B. Pre-laboratory
1. Pull out from your container the toad you brought.
2. Wash your toad using running water only. Do not apply
soap. Then, go back to your seat.
3. Pull out your dissecting set. Get the pithing needle.
4. At the dorsal part of the toad, locate the foramen
magnum.

Figure 1. Dorsal side of a toad. The pointer is pointing the location of the
foramen magnum.

5. Once located, insert the pithing needle towards the brain.


Move it up, down, and sidewards thereby destroying the
posterior part of the brain. As a result, this will paralyze
the upper limbs of the toad.
6. To paralyze the lower limbs, pull out the pithing needle
and insert again now, towards the spinal cord. Move the
pithing needle up, down, and sidewards to destroy the
spinal cord and paralyze the lower limbs.
7. Once the lower and upper limbs are paralyzed, pull out
the pins from your dissecting set.

Page 2 of 5
8. Pin the limbs of the toad to the dissecting pan facing you
the ventral side.

C. Dissection Proper
9. Using your forceps, pull the skin between the legs of the
toad.
10. Using your dissecting scissors or blade, cut the skin
sideways. Then, cut upward to the sternum and cut
sideways again to have a cut like a capital letter “I”.

Figure 2. Ventral side of the toad. The lines show where to cut.

11. Pull the skin away and pin it to the dissecting pan.
12. You may observe that the muscle surrounding the
abdomen of the toad is somehow transparent. You can
already see the internal organs.
13. Cut these transparent muscles by repeating the
process on how you cut the skin. However, your
longitudinal cut shall be 1mm from the linea alba.
14. Pin the muscles to the dissecting board.
15. Using your probe, locate the following organs:
a. Esophagus
b. Stomach
c. Small intestine
d. Large intestine

Page 3 of 5
e. Cloaca
f. Bronchial Tube
g. Lungs
h. Liver
i. Gall Bladder
j. Pancreas
k. Heart
l. Aorta
m. Ventricle
n. Atrium
o. Gastrocnemius
p. Spinal Cord
q. Spinal Column
r. Male/female?
s. Spleen
t. Linea alba
16. Your teacher will check each group of students to
determine whether or not the identification of internal
organs is correct.
17. Once the teacher is finished grading your work, start
washing with dishwashing soap, your dissecting set and
dissecting pan.
18. Put your specimen in the designated trash bin.
19. Use paper towel dry them.
20. Wait for your teacher’s further instructions.

IV. Points System

5 pts – Proper Attire


5 pts – Toad
5 pts – Proper pithing
5 pts – not over bleeding
5 pts – Specimen did not die
5 pts – Proper dissection
20 pts – Correct identification in #16
50 pts

Page 4 of 5
Prepared by:

Page 5 of 5

You might also like

pFad - Phonifier reborn

Pfad - The Proxy pFad of © 2024 Garber Painting. All rights reserved.

Note: This service is not intended for secure transactions such as banking, social media, email, or purchasing. Use at your own risk. We assume no liability whatsoever for broken pages.


Alternative Proxies:

Alternative Proxy

pFad Proxy

pFad v3 Proxy

pFad v4 Proxy