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Bio204 Solved Midterm Papers

This document contains past paper questions and answers for the Principles of Biochemical Engineering course (Bio204) at the Virtual University of Pakistan. It includes 6 multiple choice and short answer questions about topics like the relationship between product formation rate and specific growth rate in fed-batch systems, the advantages of using glass in fermenters, the functions of agitation, the definition of quasi-steady state, and the differences between axial and radial flow impellers. The document is authored by Rania Ch and shared on the VU Biologists Facebook group page.

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Muhammad Zubair
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180 views

Bio204 Solved Midterm Papers

This document contains past paper questions and answers for the Principles of Biochemical Engineering course (Bio204) at the Virtual University of Pakistan. It includes 6 multiple choice and short answer questions about topics like the relationship between product formation rate and specific growth rate in fed-batch systems, the advantages of using glass in fermenters, the functions of agitation, the definition of quasi-steady state, and the differences between axial and radial flow impellers. The document is authored by Rania Ch and shared on the VU Biologists Facebook group page.

Uploaded by

Muhammad Zubair
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Virtual University of Pakistan

Bio204
Principles of Biochemical Engineering
Midterm past papers solved
Email address
bio204@vu.edu.pk

Created By ------- Rania Ch


Group of VU Biologists

www.facebook.com/vubiologists

www.facebook.com/groups/vubiologists

VU Biologists
www.facebook.com/groups/vubiologists
Bio 204
25 June 2018, 12:00 pm
Total questions 26
20 mcq's + 6 questions

1)Describe relationship between qp and u.


Ans: Product concentration in a fed batch system over the time of the fermentation will be
dependant on the relationship between qp and u. If qp is growth related than it will change as u
thus, the product concentration remains constant. If qp is constant and independent of uthan
product concentration will start of the cycle when Dp is greater q p x but will rise with time as D
decreases and q p x become greater than Dp.

2)Why use of glass is preferable in fermenter?


Ans: On a small scale (1 to 30 L) it is possible to use glass and/or stainless steel. Glass is useful
because it gives smooth surfaces, is non-toxic, corrosion proof and it is usually easy to examine
the interior of the vessel.

3)Write two functions of agitation.

Ans: Agitation plays an important mixing and shearing role in fermentation processes. It not
only improves mass and oxygen transfer between the different phases, but also maintains
homogeneous chemical and physical conditions in the medium by continuous mixing.

4)Define quasi steady state.


Ans: The total biomass in the culture increase with time, cell concentration remains virtually
constant that is ( dx dt ) ; 0 and therefore µ ; D it is called quasi steady state.

5)What is the major difference between quasi steady state and steady state of chemostat of
fed-batch culture also write effects of values of Y, Umax and Ks.
Ans: The major difference between the quasi steady state and steady state of chemostat of a fed
batch culture is that µ is constant in the chemostate but decreases in the fed batch culture.

The effect of dillution rate on the steady state biomass and residual substrate concentrations in a
chemostate of a microorganisms with a low ks value for the limiting substrate compared with the
initial concentration.

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With increasing dilution, the residual substrate concentration increases only slightly until D
approaches umax when s increases significantly. The dilution rate at which x equal zero is
termed the critical dilution rate ( Dcrit ) and is given by the equation:

Dcrit = µmax SR \ ( KS + SR )

The kinetic characteristics of the organism are discribed by the numerical values of the constants
y, umax and ks.
The value of Y affects the steady state biomass concentration, The value of umax affects the
maximum dilution rate that may be employed and the value of Ks affects the residual substrate
concentration and also the maximum dilution rate that may be used.
The continuous culture behavior of a hypothetical bacterium with high Ks for the limiting
substrate compared with the initial limiting substrate concentration. With increasing dilution rate,
the residual substrate concentration increases significantly to support the increased growth rate.
Thus, there is a gradual increase in s and a decrease in x as D approaches Dcrit. Thus, Dcrit is
affected by the constants, µmax and Ks, and the variable, SR; the larger SR the closer is µmax.
However, µmax cannot be achieved in a simple steady state chemostat because substrate limited
conditions must always prevail.

6)Differentiate between axial and radial flow.

Ans: With radial flow mixing, the liquid flow from the impeller is initially directed towards the
wall of the reactor; i.e. along the radius of the tank.

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q With axial flow mixing, the liquid flow from the impeller is directed downwards towards the
base of the reactor, i.e. in the direction of the axis of the tank.
qRadial flow impellers are primarily used for gas-liquid contacting (such as in the mixing of
sparged bioreactors) and blending processes.
q Axial flow impellers provide more gentle but efficient mixing and are used for reactions
involving shear sensitive cells and particles.
Radial flow mixing is not as efficient as axial flow mixing.
For radial flow impellers, a much higher input of energy is required to generate a given level of
flow.
Radial flow impellers do and are designed to, generate high shear conditions. This is achieved by
the formation of vortices in the wake of the impeller.

-----------------------------------------

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