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Datasheet 238544

This document provides information on the Comet Assay Kit (3-well slides), which is a kit used to measure cellular DNA damage through a single cell gel electrophoresis assay. The kit includes comet slides, reagents such as lysis solution and agarose, and a fluorescent DNA dye to visualize DNA damage under a microscope. Images show how the comet assay works and an example of DNA damage detected in Etoposide-treated cells.

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44 views3 pages

Datasheet 238544

This document provides information on the Comet Assay Kit (3-well slides), which is a kit used to measure cellular DNA damage through a single cell gel electrophoresis assay. The kit includes comet slides, reagents such as lysis solution and agarose, and a fluorescent DNA dye to visualize DNA damage under a microscope. Images show how the comet assay works and an example of DNA damage detected in Etoposide-treated cells.

Uploaded by

Camila Pinheiro
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Product datasheet

Comet Assay Kit (3-well slides) ab238544


2 Abreviews 29 References 3 Images

Overview

Product name Comet Assay Kit (3-well slides)


Detection method Fluorescent
Product overview Comet Assay Kit (3-well slides) (ab238544) is a fast and sensitive kit for the measurement of
cellular DNA damage.

The Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of
cellular DNA damage. It is a convenient way to screen for general DNA damage, regardless of the
source or nature of the damage. Kits include Comet Slides, reagents, and a fluorescent dye to
visualize cells under an epifluorescence microscope.

Notes Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of
products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH
Authorisation, and any other relevant authorisations, for their intended uses.

Properties

Storage instructions Please refer to protocols.

Components 15 tests 75 tests

10X Lysis Solution 1 x 20ml 1 x 100ml

3-Well Comet Slides 1 x 5 slides 1 x 25 slides

Comet Agarose 1 x 15ml 1 x 15ml

EDTA Solution 1 x 50ml 1 x 250ml

Vista Green DNA Dye, 10000X 1 x 5µl 1 x 5µl

Images

1
Comet Assay Principle

Comet Assay Principle

For Data Analysis please see Section 9 in the protocol booklet.

Epifluorescence Microscopy Visualization of DNA


Damage.

Jurkat cells were untreated (left) or treated (right) with 20 µM


Etoposide for 4 hrs before performing Comet Assay (alkaline
electrophoresis conditions, 33 V/300 mA for 15 mins).

Etoposide Treatment of Jurkat Cells.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"

2
Our Abpromise to you: Quality guaranteed and expert technical support

Replacement or refund for products not performing as stated on the datasheet


Valid for 12 months from date of delivery
Response to your inquiry within 24 hours

We provide support in Chinese, English, French, German, Japanese and Spanish


Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards

If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit https://www.abcam.com/abpromise or contact our technical team.

Terms and conditions

Guarantee only valid for products bought direct from Abcam or one of our authorized distributors

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