The document summarizes several research papers that analyzed the sugar content of various honey samples using High Performance Liquid Chromatography (HPLC). Across the studies, different detectors, columns, mobile phases, and flow rates were used to separate and quantify sugars like trehalulose. The goal of the analyses was to better understand the sugar profiles and components of honeys from different regions and species of bees.
The document summarizes several research papers that analyzed the sugar content of various honey samples using High Performance Liquid Chromatography (HPLC). Across the studies, different detectors, columns, mobile phases, and flow rates were used to separate and quantify sugars like trehalulose. The goal of the analyses was to better understand the sugar profiles and components of honeys from different regions and species of bees.
The document summarizes several research papers that analyzed the sugar content of various honey samples using High Performance Liquid Chromatography (HPLC). Across the studies, different detectors, columns, mobile phases, and flow rates were used to separate and quantify sugars like trehalulose. The goal of the analyses was to better understand the sugar profiles and components of honeys from different regions and species of bees.
The document summarizes several research papers that analyzed the sugar content of various honey samples using High Performance Liquid Chromatography (HPLC). Across the studies, different detectors, columns, mobile phases, and flow rates were used to separate and quantify sugars like trehalulose. The goal of the analyses was to better understand the sugar profiles and components of honeys from different regions and species of bees.
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Nama Penulis Judul Tahun Detektor Kolom Fase Gerak Laju Alir
Zaidi et.al Analysis of 2023 evaporative light GL Sciences NH2 The HPLC mobile flow rate was fixed trehalulose in scattering column (150 mm × 4.6 phase used was a at 0.8 ml/min kelulut honey detection (ELSD) mm, oven temperature premixed 85% samples via of 40oC) acetonitrile in deionized HPLC-MS water
Bonta Victorita HIGH- 2008 refractive index analytical column acetonitrile: water The flow rate was et.al PERFORMANCE detector containing amino- (85:15, v/v) as mobile 1.3 ml/min. LIQUID modified silica gel phase CHROMATOGRA PHIC ANALYSIS OF SUGARS IN TRANSYLVANIA N HONEYDEW HONEY
ALI AGUS et al Honey sugars 2021
profile of stingless bee Tetragonula laeviceps (Hymenoptera: Meliponinae)
F. A. Vázquez‐ HPLC 2004 ED40 Micro Bio-Spin Water and 1 M NaOH flow rate of 0.4 Ortíz & E. M. Determination of electrochemical Chromatography were used as the mL/min at 88C Valenzuela‐Soto Trehalose in detector Column (Bio-Rad, eluent Selaginella Richmond, CA) with lepidophylla mixed-bed Plants
Gregory A. Hayne Quantification of 2023 RID Waters High mobile phase was flow rate of 1.0 et.al the Disaccharide Performance composed of 23: 77 mL/min Trehalose from Carbohydrate column water : acetonitrile with Biological (4.6 250 mm, 4 μm) an isocratic elution over Samples: A held at 35 o C 15 minutes Comparison of Analytical Methods (RID)
Quantification of 2023 Waters High mobile phase was flow rate of 1.4 the Disaccharide Performance composed of 16: 84 2 mL/min. Trehalose from Carbohydrate column mM ammonium acetate Biological (4.6 250 mm, 4 μm) in water : 2 mM Samples: A held at 35 o C ammonium acetate in Comparison of acetonitrile with an Analytical isocratic elution over 35 Methods minutes (LCMSMS)
Mary T. Fletcher Stingless bee 2020 Shimadzu 8045 CTO-20AC column Mobile Phase A: 70% a fowrate of 0.2 et.al honey, a novel MS/MS detector oven was operated at RO water/30% mL/min source of 35 o C acetonitrile with 0.1% trehalulose: a NH4OH biologically active disaccharide with Mobile Phase B: 20% health benefts RO water/80% acetonitrile with 0.1% NH4OH
Ivan Obreshkov Optimized 2013 ED40 detector CarboPac PA-100 mobile phase of 100 flow-rate of 0.9 et.al determination of column (4 × 250 mm) mM NaOH mL.min-1 honey carbohydrates by HPAEC-PAD
Lalita Tuksitha Antioxidant and 2018 RID Supercosil LC-NH2 mobile phase with a flow rate 1 et.al antibacterial column (25 cm × 4.6 HPLC acetonitrile/water ml/min, with an capacity of mm, 5 μm) (72,25) oven temperature stingless bee of 40 °C honey from Borneo (Sarawak)
catatan : jurnal 2, 5% madu (5mL) | Methanol 1 : 3 Aq (25 : 75 mL) jurnal 2 ada acuan malaysian standard
