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430 A. N. Vaidya et al.
I. INTRODUCTION
Product identification
CAS no. 50-21-5, 79-33-4 (L), 10326-41-7 (D)
EINECS no. 200-018-0
Formula CH3 CH(OH)COOH
Mol. wt. 90.08
H.S. code 2918.11
Toxicity Oral rat LD50: 3543 mg/kg
Synonyms 2-hydroxypropanoic acid;
1-hydroxyethanecarboxylic acid;
ethylidenelactic acid;
alpha-hydroxypropionic acid
Physical and chemical properties (99%)
Physical State Colorless to slightly yellow, syrupy liquid
Melting point 17◦ C
Boiling point 122◦ C
Specific gravity 1.2
Solubility in water Miscible
NFPA ratings Health 3, Flammability 1, Reactivity 1
Flash point 112◦ C
Stability Stable under ordinary conditions
432 A. N. Vaidya et al.
Out of these, only the synthesis from lactic acid derivatives has been
commercialized.
Lactic acid can be liberated from most of its derivatives by suitable treat-
ment, however, there is no practical advantage, as lactic acid is the raw
material for these derivatives. Lactonitrile and lactic acid nitrate are the im-
portant exceptions, as these are not produced from lactic acid. Lactonitrile
is produced from acetaldehyde and hydrogen cyanide as an intermediate in
the production of acrylonitrile, and it is further hydrolyzed to lactic acid as
follows:
HCl
CH3 CHO + HCN −→ CH3 CHOH CN (1)
Acetaldehyde lactonitrile
HCl
CH3 CHOH CN + 2H2 O −→ CH3 CHOH COONH4 (2)
Ammonium lactate
→ CH3 CHOHCOOH
lactic acid
Raw materials for lactic acid production should meet certain purity require-
ments besides being available at reasonable prices, since these are critical
for the further purification of the produced lactic acid. The choice of raw
materials largely depends on the intended application and the respective
costs of product purification. Various readily available mono- and disaccha-
ride materials are traditional substrates for lactic acid manufacturing. These
are
acid. The targets are increased growth rates, improved pH resistance, and
increased yields of lactic acid. These targets are partially achieved by ef-
forts through genetic engineering, control of metabolic activity by appro-
priate culturing techniques, and development of acid resistant mutants. A
notable patent (U.S. Patent 6,660,515, 2003) in this regard provides meth-
ods of enhancing the growth rate and/or controlling the metabolic activity
of lactic acid bacteria, which are defective in their pyruvate metabolism.
There are also starter culture compositions comprising such defective lac-
tic acid bacteria as helper organisms and lactic acid bacterial starter culture
strains. Useful helper organisms are Lactococcus strains, which are defec-
tive with respect to pyruvate formate-lyase (Pfl) and/or lactate dehydroge-
nase (Ldh) activity. The helper organisms may overexpress a gene coding
for an NAD+ -regenerating enzyme such as NADH oxidase, encoded the by
nox gene. Accordingly, invention relates in a first aspect to a method of
enhancing the growth rate and/or controlling the metabolic activity of a
lactic acid bacterial strain, comprising cultivating the strain in association
with a lactic acid bacterial helper organism that is defective in its pyruvate
metabolism.
In another recent patent (U.S. Patent 6,645,754, 2003), mutants of lac-
tic acid bacteria including Lactococcus lactis that are defective in pyru-
vate formate-lyase production and/or in their lactate dehydrogenase (Ldh)
production and methods of isolating such mutants or variants are pro-
vided. The mutants are useful in the production of food products or in
the manufacturing of compounds such as diacetyl, acetoin, and acetalde-
hyde and as components of food starter cultures, in particular Lactococ-
cus lactis DN223 deposited under accession number DSM 11036. Accord-
ingly, the invention provides a method of isolating a pyruvate formate-
lyase (Pfl) defective lactic acid bacterium, with the method comprising the
steps of
1. Providing a wild-type lactic acid bacterial strain that under aerobic con-
ditions is not capable of growth in the absence of acetate in a medium
not containing lipoic acid, but that is capable of growth is such medium
under anaerobic conditions.
2. Selecting from said wild-type strain a mutant that under said conditions
essentially does not grow in the absence of acetate.
bacteria
−→ CH3 COCOOH −→ CH3 CHOHCOOH (5)
Pyruvic acid Latic acid
(8)
(9)
(10)
lactic acid and cation. This can be achieved below a pH of 3.86 as the pK a
value for lactic acid is 3.86 and above this pH lactate is virtually present as
a salt. If calcium is used as the cation for maintaining the pH during fermen-
tation, then acidification with sulfuric acid results in precipitation of calcium
sulfate. Likewise, a number of processes can be used to remove cations.
For example, if sodium is used as the cation in fermentation, then it can
be removed by ion exchange. Removal of lactic acid can also be effected by
esterification of lactic acid with alcohols, by liquid–liquid extraction, or by ad-
sorption. Membrane processes, distillation, evaporation, crystallization, and
so on can achieve the concentration of the separated lactic acid. Separation
and purification can also be achieved by chromatography and ion exchange.
iron ions, the depolarization of the “crude” lactic acid thus obtained with
activated charcoal, and after the subsequent purification steps to remove all
the residual salts, concentration by evaporation, and hence crystallization of
the lactates. Here again, this process suffers due to a large number of purifi-
cation steps and the handling of toxic chemicals. The latest patented process
for the preparation of high-purity lactic acid from an aqueous solution con-
taining lactic acid in the form of salt(s) is characterized in that the aqueous
solution is treated with a strong acid in order to liberate lactic acid in the free
form, and to produce corresponding salts of the strong acid, the salts of the
strong acid are crystallized by evaporative crystallization and the lactic acid
is recovered in the free form in solution (U.S. Patent 6,384,276, 2002).
A general schematic for all such processes is shown in Figure 1.
It has been reported that distribution coefficients for the first type of
extractants are very low, while those for the second type extractants are not
high enough to extract lactic acid efficiently. Extractants from the third type,
high-molecular-weight amines, are the most effective ones. The high basic-
ity of amines results in reactive extraction of lactic acid, which increases the
extraction efficiency substantially. Any solvent that has a high enough capac-
ity for lactic acid to be economic, such as amyl alcohol or a tertiary amine,
will also coextract some water, salts, and other organic acids. Thus, extrac-
tion alone does not economically produce lactic acid of high enough pu-
rity. Several extractants such as isopropyl ether (U.S. Patent 1,906,068, 1931)
α, ω-diamino-oligoethers (Miesiac et al., 1992), isobutanol (German Patent
3415141, 1985), and trialkyl tertiary amines in an organic solvent (U.S. Patent
4698303, such as) di-n-octylamine and n-hexane (Hano et al., 1993), have
been described. As a novel improvement, the application of liquid mem-
branes should be mentioned (Chaudhari and Pyle, 1992a, 1992b; Scholler
et al., 1993; Lazarova and Peeva, 1994). Bar and Geiner (1987) studied the
feasibility of extracting lactic acid from aqueous solution by means of a long-
chain trialkyl amine of low basicity, such as tridodecylamine, using various
tridodecylamine solutions in n-dodecanol. It was found that extraction of lac-
tic acid with a long-chain trialkyl amine such as tridodecylamine was effective
only at a pH that is lower than the pK a of lactic acid, which is 3.86. At such
a low pH, however, the lactic acid-fermenting microorganisms such as, for
example, Lactobacillus delbrueckii or Lactobacillus acidophilus are severely
inhibited. In addition, the extraction of lactic acid with amines produces the
Production and Recovery of Lactic Acid for Polylactide 443
salt of lactic acid with amine, and not pure lactic acid. Obviously, lactic acid
and amine should be recovered back from the salt, and several methods
are suggested for this salt splitting. These methods include back-extraction
with water, back-extraction with another amine, thermal splitting, and inert
gas-induced splitting. Applying these methods, pure lactic acid is obtained
and the recovered amine is recycled back to extraction. In effect, extraction
of lactic acid with amines becomes at least two or many times a multistep
downstream process.
U.S. Patent 5,132,456, 1992, describes a process for recovering carboxylic
acid from a carboxylic acid-containing aqueous feed stream having a pH close
to or above the pK a level of the acid. The recovery process involves what
may be described as a cascade-type acid withdrawal operation, in which the
basicity of the extractant is increased stepwise. In the second stage, carboxylic
acid is back-extracted either by ammonia or low-molecular-weight tertiary
amine, referred to as secondary extractant, which results in formation of
lactate salt with ammonia or tertiary amine. This salt is then thermally split to
yield back pure acid and secondary extractant. Applying this process to lactic
acid involves the formation of salts of lactic acid with strong bases having
a pK a value of about 9–11. Thus, the decomposition of these salts into free
lactic acid is energy-intensive. The patent also mentions the use of Alamine
336 (tricaprylylamine) for the extraction of, among others, lactic acid from an
aqueous solution, but no yields are mentioned. With the extraction of even
small quantities of lactic acid from a fermentation broth, the pH of the broth
rises rapidly to above 7, while the pK a of an extractant based on Alamine
336 is less than 6. As shown in the patent, the uptake of carboxylic acids
from aqueous solutions drops rapidly with an increase of pH. It is therefore
inherent that the lactic acid uptake, if any, is negligible. It is further noted that
upon heat treatment and concentration of an ammonium lactate, crystalline
lactic acid does not precipitate; instead, the viscosity of the solutions increases
steadily as a result of self-association of the acid. Baniel et al. (U.S. Patent
4,275,234, 1981), found that the extracted acid can be recovered from the
acid comprising extractant by back-extraction with water. They have also
found that if the back-extraction is conducted at a temperature higher than
that of the extraction, the concentration of the acid in the back-extract (the
aqueous product of the back-extraction) could be significantly higher than
that of the aqueous feed to the process. Yet if the concentration of the acid
in the feed is very low, the concentration of the back-extract could still be
too low. That is particularly true when the feed consists mainly of the salt
of the acid rather than the free acid. Acidulating neutral fermentation liquors
by the addition of acids for recovery via ester formation or other methods
results in the formation of by-product salts, such as gypsum in the case of
calcium lactate acidulation with sulfuric acid or sodium, or ammonium sulfate
in others. Reagents are consumed, and disposal of undesired by-products is
required. Efforts have recently been made to recover the lactic acid from
444 A. N. Vaidya et al.
said to yield lactic acid of optimum purity without any waste product to
be disposed of. Disadvantages of this method are the expensive equipment
and technical problems in handling a fluid with higher contents of inorganic
compounds.
446 A. N. Vaidya et al.
salts of the strong acid and an impure lactate ester of the alcohol; (2) re-
moving water from the mixture as a water/alcohol azeotrope, which can be
accomplished either sequentially or substantially simultaneously with step 1;
(3) removing the crystal precipitate from the mixture; (4) distilling the impure
lactate ester to remove the impurities; and (5) recovering the high-purity ester.
It can be seen that the processes of the esterification suffer in practice
from succession of numerous and cumbersome steps that make the purifica-
tion of lactic acid from an aqueous solution containing lactic acid in the form
of salt(s) long and tedious. A general flow diagram for lactic acid recovery
by esterification is shown in Figure 3.
(11)
(CH3 CHOHCOO)2 Ca + H2 SO4 → 2CH3 CHOHCOOH + CaSO4 (12)
Sulfuric acid Lactic acid gypsum
factors is very small. Often the costs of nutrients are more than the sugar feed-
stock. In addition, part of the nutrients not bound to the growing biomass
remain in the product, thus lowering its purity. It has been recently reported
that in the preparation of lactic acid, the actual fermentation reaction and
the culturing of producer organisms can be separated into discrete produc-
tion and refreshing cycles. In the culturing stage, that is, refreshing cycle, the
rich nutrient medium is passed through the bioreactor for a few hours. After
the culturing stage, pure feedstock solution that reacts into lactic acid can
be passed through the bioreactor. A carbohydrate, such as starch, or other
polysaccharide, such as polydextrose or inulin, or sucrose, lactose, or glu-
cose, or other mono-, di-, or oligosaccharides or a mixture of these may be
used as feedstock (U.S. Patent 5,932,455, 1999, U.S. Patent 4698303, 1987).
Researcher/institution/industry,
international status Year Salient development
limited due to the high costs associated with its production, primarily energy
costs, making it uncompetitive with similar nonbiodegradable petroleum-
based polymers and polyesters.
FIGURE 7. Block diagram for the production of polylactic acid (PLA), a green polymer.
cost of production and processing of PLA for commercial use. The com-
mercial items made from PLA include packaging materials, computer cases,
paper coatings, fibers, garbage bags, and automobile parts.
PLA is a green plastic that is produced from renewable resource such as plant
starch. It is biodegradable and is likely to minimize the disposal problems.
Thus it can be viewed as an environmentally friendly plastic. The life cycle
of PLA is shown in Figure 9. However, its real impact on the environment
should be assessed in detail with regard to:
Monsanto and Cargill Dow, USA, have formulated strategies for deriving
energy from biomass. Monsanto proposes to burn all the corm stover that
remains after extraction of plastic, to generate electricity. Thus, it seems that
utilization of biomass-derived electricity is possible and more than enough
to meet the power requirement of PLA extraction.
The conventional plastics are not biodegradable, but they have broader range
of material properties when compared to PLA. Biodegradability, helps to re-
lieve the problem of solid-waste disposal, but degradation gives off green-
house gases, thereby compromising air quality. Although PLA production
uses fewer fossil resources than its petrochemical counterparts, it still re-
quires more energy and emits more greenhouse gases during manufacture.
IX. DISCUSSION
X. RESEARCH NEEDS
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